Restriction map for
pF1KA0058
(Circular) (Six-base) MAPSORT of: pF1KA0058.seq Check: 4615 from: 1 to: 3617
pF1KA0058 3617 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 234 433 234
Size: 199 3418
AccI GT'mk_AC
Cuts at: 173 655 173
Size: 482 3135
Acc65I G'GTAC_C
Cuts at: 633 633
Size: 3617
AclI AA'CG_TT
Cuts at: 3457 3457
Size: 3617
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1442 1874 2621 2889 1442
Size: 432 747 268 2170
Fragments arranged by size:
2170 747 432 268
AflIII A'CryG_T
Cuts at: 969 2073 3050 969
Size: 1104 977 1536
Fragments arranged by size:
1536 1104 977
AgeI A'CCGG_T
Cuts at: 2005 2005
Size: 3617
AlwNI CAG_nnn'CTG
Cuts at: 271 922 2489 271
Size: 651 1567 1399
Fragments arranged by size:
1567 1399 651
ApaLI G'TGCA_C
Cuts at: 259 2387 259
Size: 2128 1489
ApoI r'AATT_y
Cuts at: 621 2970 3226 621
Size: 2349 256 1012
Fragments arranged by size:
2349 1012 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3617
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3617
AvaI C'yCGr_G
Cuts at: 637 637
Size: 3617
BamHI G'GATC_C
Cuts at: 642 642
Size: 3617
BanI G'GyrC_C
Cuts at: 445 633 1047 1295 1330 445
Size: 188 414 248 35 2732
Fragments arranged by size:
2732 414 248 188 35
BanII G_rGCy'C
Cuts at: 242 631 1661 242
Size: 389 1030 2198
Fragments arranged by size:
2198 1030 389
BbeI G_GCGC'C
Cuts at: 1051 1299 1051
Size: 248 3369
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 240 274 240
Size: 34 3583
BciVI GTATCCnnnnn_n'
Cuts at: 1515 2276 3617 1515
Size: 761 1341 1515
Fragments arranged by size:
1515 1341 761
BclI T'GATC_A
Cuts at: 1137 1137
Size: 3617
BglII A'GATC_T
Cuts at: 1132 3169 1132
Size: 2037 1580
BlpI GC'TnA_GC
Cuts at: 518 729 518
Size: 211 3406
Bme1580I G_kGCm'C
Cuts at: 263 1242 1335 2391 263
Size: 979 93 1056 1489
Fragments arranged by size:
1489 1056 979 93
BmrI ACTGGGnnnn_n'
Cuts at: 327 829 910 1245 3389 327
Size: 502 81 335 2144 555
Fragments arranged by size:
2144 555 502 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1984 1984
Size: 3617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 326
Size: 3617
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 1672 2164 2462 793
Size: 879 492 298 1948
Fragments arranged by size:
1948 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3321 3321
Size: 3617
BsaAI yAC'GTr
Cuts at: 970 970
Size: 3617
BsaBI GATnn'nnATC
Cuts at: 6 1131 3168 6
Size: 1125 2037 455
Fragments arranged by size:
2037 1125 455
BsaHI Gr'CG_yC
Cuts at: 1048 1296 1998 3494 1048
Size: 248 702 1496 1171
Fragments arranged by size:
1496 1171 702 248
BsaWI w'CCGG_w
Cuts at: 806 1327 2005 2279 2426 2837 806
Size: 521 678 274 147 411 1586
Fragments arranged by size:
1586 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 204 234 530 560 204
Size: 30 296 30 3261
Fragments arranged by size:
3261 296 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 476 476
Size: 3617
BseYI C'CCAG_C
Cuts at: 460 1042 1800 2377 2873 460
Size: 582 758 577 496 1204
Fragments arranged by size:
1204 758 582 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 172
Size: 3617
BsiEI CG_ry'CG
Cuts at: 104 1159 1205 2413 104
Size: 1055 46 1208 1308
Fragments arranged by size:
1308 1208 1055 46
BsiHKAI G_wGCw'C
Cuts at: 242 263 631 1409 1599 2391 242
Size: 21 368 778 190 792 1468
Fragments arranged by size:
1468 792 778 368 190 21
Bsp1286I G_dGCh'C
Cuts at: 242 263 631 1242 1335 1409 1599 1661 2391
Size: 21 368 611 93 74 190 62 730
Cuts at: 2391 242
Size: 1468
Fragments arranged by size:
1468 730 611 368 190 93 74 62
21
BspEI T'CCGG_A
Cuts at: 2837 2837
Size: 3617
BspHI T'CATG_A
Cuts at: 2793 2793
Size: 3617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 234 433 667 881 1183 1564 234
Size: 199 234 214 302 381 2287
Fragments arranged by size:
2287 381 302 234 214 199
BsrBI CCG'CTC
Cuts at: 50 1909 1963 3447 3612 50
Size: 1859 54 1484 165 55
Fragments arranged by size:
1859 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1529 1529
Size: 3617
BsrFI r'CCGG_y
Cuts at: 1615 1796 2005 1615
Size: 181 209 3227
Fragments arranged by size:
3227 209 181
BssSI C'ACGA_G
Cuts at: 1888 2246 1888
Size: 358 3259
BstAPI GCAn_nnn'nTGC
Cuts at: 994 994
Size: 3617
BstBI TT'CG_AA
Cuts at: 1978 1978
Size: 3617
BstXI CCAn_nnnn'nTGG
Cuts at: 420 420
Size: 3617
BstYI r'GATC_y
Cuts at: 1 642 1109 1132 1467 1713 2725 2811 2823
Size: 641 467 23 335 246 1012 86 12
Cuts at: 2823 3169 1
Size: 346 449
Fragments arranged by size:
1012 641 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 174 174
Size: 3617
Bsu36I CC'TnA_GG
Cuts at: 197 197
Size: 3617
BtgI C'CryG_G
Cuts at: 326 371 326
Size: 45 3572
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1567 1748 1911 1567
Size: 181 163 3273
Fragments arranged by size:
3273 181 163
BtsI GCAGTG_nn'
Cuts at: 860 860
Size: 3617
DraI TTT'AAA
Cuts at: 616 616
Size: 3617
DrdI GACnn_nn'nnGTC
Cuts at: 1323 2181 1323
Size: 858 2759
EaeI y'GGCC_r
Cuts at: 1202 1376 1767 1794 3139 1202
Size: 174 391 27 1345 1680
Fragments arranged by size:
1680 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1202 1202
Size: 3617
EarI CTCTTCn'nnn_
Cuts at: 1640 1850 1640
Size: 210 3407
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1825 2135 2281 3427 1825
Size: 310 146 1146 2015
Fragments arranged by size:
2015 1146 310 146
EcoICRI GAG'CTC
Cuts at: 240 629 240
Size: 389 3228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 326 1442 1874 2621 2889 326
Size: 1116 432 747 268 1054
Fragments arranged by size:
1116 1054 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 756 756
Size: 3617
EcoRI G'AATT_C
Cuts at: 621 621
Size: 3617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 588 1636 588
Size: 1048 2569
FspI TGC'GCA
Cuts at: 1398 1398
Size: 3617
HaeII r_GCGC'y
Cuts at: 1051 1299 2321 3300 1051
Size: 248 1022 979 1368
Fragments arranged by size:
1368 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 174 206 530 562 1459 1489 1491 1521 174
Size: 32 324 32 897 30 2 30 2270
Fragments arranged by size:
2270 897 324 32 32 30 30 2
HincII GTy'rAC
Cuts at: 656 656
Size: 3617
KasI G'GCGC_C
Cuts at: 1047 1295 1047
Size: 248 3369
KpnI G_GTAC'C
Cuts at: 637 637
Size: 3617
MluI A'CGCG_T
Cuts at: 3050 3050
Size: 3617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 154 2288 2472 154
Size: 2134 184 1299
Fragments arranged by size:
2134 1299 184
MscI TGG'CCA
Cuts at: 1378 1378
Size: 3617
MslI CAynn'nnrTG
Cuts at: 1606 1733 1606
Size: 127 3490
MspA1I CmG'CkG
Cuts at: 561 728 1042 1402 2415 2660 561
Size: 167 314 360 1013 245 1518
Fragments arranged by size:
1518 1013 360 314 245 167
NaeI GCC'GGC
Cuts at: 1798 1798
Size: 3617
NarI GG'CG_CC
Cuts at: 1048 1296 1048
Size: 248 3369
NcoI C'CATG_G
Cuts at: 326 371 326
Size: 45 3572
NgoMIV G'CCGG_C
Cuts at: 1796 1796
Size: 3617
NspI r_CATG'y
Cuts at: 670 1170 2077 670
Size: 500 907 2210
Fragments arranged by size:
2210 907 500
PasI CC'CwG_GG
Cuts at: 265 265
Size: 3617
PciI A'CATG_T
Cuts at: 2073 2073
Size: 3617
PflMI CCAn_nnn'nTGG
Cuts at: 339 496 339
Size: 157 3460
PfoI T'CCnGG_A
Cuts at: 303 495 303
Size: 192 3425
PmeI GTTT'AAAC
Cuts at: 616 616
Size: 3617
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2756 2788 2756
Size: 32 3585
PstI C_TGCA'G
Cuts at: 664 1349 664
Size: 685 2932
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3617
PvuII CAG'CTG
Cuts at: 1042 1402 1042
Size: 360 3257
RsrII CG'GwC_CG
Cuts at: 1812 1812
Size: 3617
SacI G_AGCT'C
Cuts at: 242 631 242
Size: 389 3228
SalI G'TCGA_C
Cuts at: 654 654
Size: 3617
SapI GCTCTTCn'nnn_
Cuts at: 1640 1850 1640
Size: 210 3407
SbfI CC_TGCA'GG
Cuts at: 664 664
Size: 3617
ScaI AGT'ACT
Cuts at: 3134 3134
Size: 3617
SfcI C'TryA_G
Cuts at: 33 660 1345 2338 2529 33
Size: 627 685 993 191 1121
Fragments arranged by size:
1121 993 685 627 191
SfoI GGC'GCC
Cuts at: 1049 1297 1049
Size: 248 3369
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3617
SmaI CCC'GGG
Cuts at: 639 639
Size: 3617
SmlI C'TyrA_G
Cuts at: 772 1687 2179 2441 772
Size: 915 492 262 1948
Fragments arranged by size:
1948 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3617
SphI G_CATG'C
Cuts at: 670 1170 670
Size: 500 3117
StyI C'CwwG_G
Cuts at: 326 371 751 1105 326
Size: 45 380 354 2838
Fragments arranged by size:
2838 380 354 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 278 379 571 1200 1333 1792 2001 278
Size: 101 192 629 133 459 209 1894
Fragments arranged by size:
1894 629 459 209 192 133 101
TatI w'GTAC_w
Cuts at: 3132 3132
Size: 3617
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3134 3166 3134
Size: 32 3585
Tth111I GACn'n_nGTC
Cuts at: 1414 1414
Size: 3617
XbaI T'CTAG_A
Cuts at: 648 648
Size: 3617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 369 420 1112 369
Size: 51 692 2874
Fragments arranged by size:
2874 692 51
XmaI C'CCGG_G
Cuts at: 637 637
Size: 3617
XmnI GAAnn'nnTTC
Cuts at: 574 574
Size: 3617
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI