Restriction map for
pF1KA0044
(Circular) (Six-base) MAPSORT of: pF1KA0044.seq Check: 4552 from: 1 to: 4538
pF1KA0044 4538 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 863 1576 863
Size: 713 3825
Acc65I G'GTAC_C
Cuts at: 1554 1554
Size: 4538
AclI AA'CG_TT
Cuts at: 454 4378 454
Size: 3924 614
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1279 2363 2795 3148 3810 1279
Size: 1084 432 353 662 2007
Fragments arranged by size:
2007 1084 662 432 353
AflII C'TTAA_G
Cuts at: 501 501
Size: 4538
AflIII A'CryG_T
Cuts at: 383 1890 3690 3971 383
Size: 1507 1800 281 950
Fragments arranged by size:
1800 1507 950 281
AhdI GACnn_n'nnGTC
Cuts at: 968 968
Size: 4538
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1043 1075 1043
Size: 32 4506
AlwNI CAG_nnn'CTG
Cuts at: 1843 3281 1843
Size: 1438 3100
ApaLI G'TGCA_C
Cuts at: 3376 3376
Size: 4538
ApoI r'AATT_y
Cuts at: 427 493 652 1033 1542 3891 4147 427
Size: 66 159 381 509 2349 256 818
Fragments arranged by size:
2349 818 509 381 256 159 66
AseI AT'TA_AT
Cuts at: 20 773 20
Size: 753 3785
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
AvaI C'yCGr_G
Cuts at: 605 1558 605
Size: 953 3585
AvrII C'CTAG_G
Cuts at: 657 657
Size: 4538
BamHI G'GATC_C
Cuts at: 1563 1563
Size: 4538
BanI G'GyrC_C
Cuts at: 1554 1968 2216 2251 1554
Size: 414 248 35 3841
Fragments arranged by size:
3841 414 248 35
BanII G_rGCy'C
Cuts at: 587 1116 1552 2582 587
Size: 529 436 1030 2543
Fragments arranged by size:
2543 1030 529 436
BbeI G_GCGC'C
Cuts at: 1972 2220 1972
Size: 248 4290
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1158 1192 1158
Size: 34 4504
BciVI GTATCCnnnnn_n'
Cuts at: 2436 3492 4538 2436
Size: 1056 1046 2436
Fragments arranged by size:
2436 1056 1046
BclI T'GATC_A
Cuts at: 186 335 555 2058 186
Size: 149 220 1503 2666
Fragments arranged by size:
2666 1503 220 149
BglII A'GATC_T
Cuts at: 2053 4090 2053
Size: 2037 2501
BlpI GC'TnA_GC
Cuts at: 1650 1650
Size: 4538
Bme1580I G_kGCm'C
Cuts at: 2163 2256 3380 2163
Size: 93 1124 3321
Fragments arranged by size:
3321 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1750 1831 2166 4310 1750
Size: 81 335 2144 1978
Fragments arranged by size:
2144 1978 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1533 2905 1533
Size: 1372 3166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 1068 503
Size: 565 3973
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 684 1714 2593 3307 3605 684
Size: 1030 879 714 298 1617
Fragments arranged by size:
1617 1030 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4242 4242
Size: 4538
BsaAI yAC'GTr
Cuts at: 1891 1891
Size: 4538
BsaBI GATnn'nnATC
Cuts at: 6 2052 4089 6
Size: 2046 2037 455
Fragments arranged by size:
2046 2037 455
BsaHI Gr'CG_yC
Cuts at: 1969 2217 2919 4415 1969
Size: 248 702 1496 2092
Fragments arranged by size:
2092 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1727 2248 2926 3337 3484 3758 1727
Size: 521 678 411 147 274 2507
Fragments arranged by size:
2507 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 872 1963 2721 3386 3794 872
Size: 1091 758 665 408 1616
Fragments arranged by size:
1616 1091 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 913 913
Size: 4538
BsiEI CG_ry'CG
Cuts at: 104 1530 2080 2126 3356 104
Size: 1426 550 46 1230 1286
Fragments arranged by size:
1426 1286 1230 550 46
BsiHKAI G_wGCw'C
Cuts at: 587 806 1116 1552 2330 2520 3380 587
Size: 219 310 436 778 190 860 1745
Fragments arranged by size:
1745 860 778 436 310 219 190
Bsp1286I G_dGCh'C
Cuts at: 587 806 1116 1552 2163 2256 2330 2520 2582
Size: 219 310 436 611 93 74 190 62
Cuts at: 2582 3380 587
Size: 798 1745
Fragments arranged by size:
1745 798 611 436 310 219 190 93
74 62
BspHI T'CATG_A
Cuts at: 1142 2970 1142
Size: 1828 2710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1588 1802 2104 2485 1588
Size: 214 302 381 3641
Fragments arranged by size:
3641 381 302 214
BsrBI CCG'CTC
Cuts at: 50 424 2830 2884 4368 4533 50
Size: 374 2406 54 1484 165 55
Fragments arranged by size:
2406 1484 374 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2450 2450
Size: 4538
BsrFI r'CCGG_y
Cuts at: 2536 2717 2536
Size: 181 4357
BsrGI T'GTAC_A
Cuts at: 1300 1300
Size: 4538
BssSI C'ACGA_G
Cuts at: 2809 3517 2809
Size: 708 3830
BstAPI GCAn_nnn'nTGC
Cuts at: 1915 1915
Size: 4538
BstBI TT'CG_AA
Cuts at: 390 2899 390
Size: 2509 2029
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4538
BstYI r'GATC_y
Cuts at: 1 600 1413 1563 2030 2053 2388 2634 2940
Size: 599 813 150 467 23 335 246 306
Cuts at: 2940 2952 3038 4090 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 813 599 467 449 335 306 246
150 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1460 1460
Size: 4538
BtgI C'CryG_G
Cuts at: 107 149 107
Size: 42 4496
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2488 2669 2832 2488
Size: 181 163 4194
Fragments arranged by size:
4194 181 163
BtsI GCAGTG_nn'
Cuts at: 1494 1781 1494
Size: 287 4251
DraI TTT'AAA
Cuts at: 1537 1537
Size: 4538
DraIII CAC_nnn'GTG
Cuts at: 259 846 259
Size: 587 3951
DrdI GACnn_nn'nnGTC
Cuts at: 2244 3588 2244
Size: 1344 3194
EaeI y'GGCC_r
Cuts at: 122 955 1070 1527 2123 2297 2688 2715 4060
Size: 833 115 457 596 174 391 27 1345
Cuts at: 4060 122
Size: 600
Fragments arranged by size:
1345 833 600 596 457 391 174 115
27
EagI C'GGCC_G
Cuts at: 1527 2123 1527
Size: 596 3942
EarI CTCTTCn'nnn_
Cuts at: 794 998 1063 1270 2561 2771 794
Size: 204 65 207 1291 210 2561
Fragments arranged by size:
2561 1291 210 207 204 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2746 3488 3634 4348 2746
Size: 742 146 714 2936
Fragments arranged by size:
2936 742 714 146
EcoICRI GAG'CTC
Cuts at: 585 1114 1550 585
Size: 529 436 3573
Fragments arranged by size:
3573 529 436
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 1068 1279 2363 2795 3148 3810 503
Size: 565 211 1084 432 353 662 1231
Fragments arranged by size:
1231 1084 662 565 432 353 211
EcoO109I rG'GnC_Cy
Cuts at: 1464 1677 1464
Size: 213 4325
EcoRI G'AATT_C
Cuts at: 1542 1542
Size: 4538
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 214 831 1500 2557 214
Size: 617 669 1057 2195
Fragments arranged by size:
2195 1057 669 617
FspI TGC'GCA
Cuts at: 2319 2319
Size: 4538
HaeII r_GCGC'y
Cuts at: 1972 2220 3450 4221 1972
Size: 248 1230 771 2289
Fragments arranged by size:
2289 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 659 691 954 986 2380 2410 2412 2442 659
Size: 32 263 32 1394 30 2 30 2755
Fragments arranged by size:
2755 1394 263 32 32 30 30 2
HincII GTy'rAC
Cuts at: 381 1577 381
Size: 1196 3342
HindIII A'AGCT_T
Cuts at: 196 196
Size: 4538
HpaI GTT'AAC
Cuts at: 381 381
Size: 4538
KasI G'GCGC_C
Cuts at: 1968 2216 1968
Size: 248 4290
KpnI G_GTAC'C
Cuts at: 1558 1558
Size: 4538
MluI A'CGCG_T
Cuts at: 3971 3971
Size: 4538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 552 3297 3481 552
Size: 2745 184 1609
Fragments arranged by size:
2745 1609 184
MscI TGG'CCA
Cuts at: 957 1072 2299 957
Size: 115 1227 3196
Fragments arranged by size:
3196 1227 115
MslI CAynn'nnrTG
Cuts at: 112 731 1273 2527 2654 112
Size: 619 542 1254 127 1996
Fragments arranged by size:
1996 1254 619 542 127
MspA1I CmG'CkG
Cuts at: 876 1649 1963 2323 3107 3352 876
Size: 773 314 360 784 245 2062
Fragments arranged by size:
2062 784 773 360 314 245
NaeI GCC'GGC
Cuts at: 2719 2719
Size: 4538
NarI GG'CG_CC
Cuts at: 1969 2217 1969
Size: 248 4290
NcoI C'CATG_G
Cuts at: 107 107
Size: 4538
NdeI CA'TA_TG
Cuts at: 368 368
Size: 4538
NgoMIV G'CCGG_C
Cuts at: 2717 2717
Size: 4538
NspI r_CATG'y
Cuts at: 387 1591 2091 3694 387
Size: 1204 500 1603 1231
Fragments arranged by size:
1603 1231 1204 500
PciI A'CATG_T
Cuts at: 383 3690 383
Size: 3307 1231
PflMI CCAn_nnn'nTGG
Cuts at: 150 150
Size: 4538
PmeI GTTT'AAAC
Cuts at: 1537 1537
Size: 4538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2984 3016 2984
Size: 32 4506
PpuMI rG'GwC_Cy
Cuts at: 1464 1464
Size: 4538
PstI C_TGCA'G
Cuts at: 1501 1585 2270 1501
Size: 84 685 3769
Fragments arranged by size:
3769 685 84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4538
PvuII CAG'CTG
Cuts at: 876 1963 2323 876
Size: 1087 360 3091
Fragments arranged by size:
3091 1087 360
RsrII CG'GwC_CG
Cuts at: 2733 2733
Size: 4538
SacI G_AGCT'C
Cuts at: 587 1116 1552 587
Size: 529 436 3573
Fragments arranged by size:
3573 529 436
SalI G'TCGA_C
Cuts at: 1575 1575
Size: 4538
SapI GCTCTTCn'nnn_
Cuts at: 794 1270 2561 2771 794
Size: 476 1291 210 2561
Fragments arranged by size:
2561 1291 476 210
SbfI CC_TGCA'GG
Cuts at: 1585 1585
Size: 4538
ScaI AGT'ACT
Cuts at: 4055 4055
Size: 4538
SfcI C'TryA_G
Cuts at: 33 478 1497 1581 2266 3234 3425 33
Size: 445 1019 84 685 968 191 1146
Fragments arranged by size:
1146 1019 968 685 445 191 84
SfoI GGC'GCC
Cuts at: 1970 2218 1970
Size: 248 4290
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
SmaI CCC'GGG
Cuts at: 1560 1560
Size: 4538
SmlI C'TyrA_G
Cuts at: 501 663 1693 2608 3322 3584 501
Size: 162 1030 915 714 262 1455
Fragments arranged by size:
1455 1030 915 714 262 162
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4538
SphI G_CATG'C
Cuts at: 1591 2091 1591
Size: 500 4038
SspI AAT'ATT
Cuts at: 758 758
Size: 4538
StyI C'CwwG_G
Cuts at: 107 657 1484 1672 2026 107
Size: 550 827 188 354 2619
Fragments arranged by size:
2619 827 550 354 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 334 2121 2254 2713 2922 334
Size: 1787 133 459 209 1950
Fragments arranged by size:
1950 1787 459 209 133
TatI w'GTAC_w
Cuts at: 1300 4053 1300
Size: 2753 1785
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4055 4087 4055
Size: 32 4506
Tth111I GACn'n_nGTC
Cuts at: 2335 2335
Size: 4538
XbaI T'CTAG_A
Cuts at: 1569 1569
Size: 4538
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1482 2033 1482
Size: 551 3987
XmaI C'CCGG_G
Cuts at: 1558 1558
Size: 4538
XmnI GAAnn'nnTTC
Cuts at: 1264 1264
Size: 4538
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AleI AlfI ApaI AscI
BaeI BbsI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BseRI BsiWI BsmI BsmBI BspEI BssHII BstEII
BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI MfeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI