Restriction map for
pF1KA0042
(Circular) (Six-base) MAPSORT of: pF1KA0042.seq Check: 9071 from: 1 to: 8057
pF1KA0042 8057 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AccI GT'mk_AC
Cuts at: 5095 5095
Size: 8057
Acc65I G'GTAC_C
Cuts at: 5073 5073
Size: 8057
AclI AA'CG_TT
Cuts at: 7897 7897
Size: 8057
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 809 1039 3635 4408 4737 4880 5882 6314
Size: 448 230 2596 773 329 143 1002 432
Cuts at: 6314 7061 7329 361
Size: 747 268 1089
Fragments arranged by size:
2596 1089 1002 773 747 448 432 329
268 230 143
AfeI AGC'GCT
Cuts at: 1933 1933
Size: 8057
AflII C'TTAA_G
Cuts at: 214 742 994 2358 214
Size: 528 252 1364 5913
Fragments arranged by size:
5913 1364 528 252
AflIII A'CryG_T
Cuts at: 457 1197 5409 6513 7490 457
Size: 740 4212 1104 977 1024
Fragments arranged by size:
4212 1104 1024 977 740
AgeI A'CCGG_T
Cuts at: 6445 6445
Size: 8057
AleI CACnn'nnGTG
Cuts at: 1834 1834
Size: 8057
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3305 3305
Size: 8057
AlwNI CAG_nnn'CTG
Cuts at: 493 2336 5362 6929 493
Size: 1843 3026 1567 1621
Fragments arranged by size:
3026 1843 1621 1567
ApaLI G'TGCA_C
Cuts at: 6827 6827
Size: 8057
ApoI r'AATT_y
Cuts at: 913 1610 1858 2112 2667 2881 3254 3305 3701
Size: 697 248 254 555 214 373 51 396
Cuts at: 3701 3744 3801 3827 3926 4342 4612 5061 7410
Size: 43 57 26 99 416 270 449 2349
Cuts at: 7410 7666 913
Size: 256 1304
Fragments arranged by size:
2349 1304 697 555 449 416 396 373
270 256 254 248 214 99 57 51
43 26
AseI AT'TA_AT
Cuts at: 20 1985 3911 20
Size: 1965 1926 4166
Fragments arranged by size:
4166 1965 1926
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8057
AvaI C'yCGr_G
Cuts at: 5077 5077
Size: 8057
BamHI G'GATC_C
Cuts at: 5082 5082
Size: 8057
BanI G'GyrC_C
Cuts at: 5073 5487 5735 5770 5073
Size: 414 248 35 7360
Fragments arranged by size:
7360 414 248 35
BanII G_rGCy'C
Cuts at: 1003 3367 4991 5017 5071 6101 1003
Size: 2364 1624 26 54 1030 2959
Fragments arranged by size:
2959 2364 1624 1030 54 26
BbeI G_GCGC'C
Cuts at: 5491 5739 5491
Size: 248 7809
BbsI GAAGACnn'nnnn_
Cuts at: 437 1242 3236 437
Size: 805 1994 5258
Fragments arranged by size:
5258 1994 805
BbvCI CC'TCA_GC
Cuts at: 989 989
Size: 8057
BciVI GTATCCnnnnn_n'
Cuts at: 5955 6716 8057 5955
Size: 761 1341 5955
Fragments arranged by size:
5955 1341 761
BclI T'GATC_A
Cuts at: 2649 5577 2649
Size: 2928 5129
BglII A'GATC_T
Cuts at: 271 1524 1914 4501 5572 7609 271
Size: 1253 390 2587 1071 2037 719
Fragments arranged by size:
2587 2037 1253 1071 719 390
BlpI GC'TnA_GC
Cuts at: 3002 5169 3002
Size: 2167 5890
Bme1580I G_kGCm'C
Cuts at: 5682 5775 6831 5682
Size: 93 1056 6908
Fragments arranged by size:
6908 1056 93
BmgBI CAC'GTC
Cuts at: 460 460
Size: 8057
BmrI ACTGGGnnnn_n'
Cuts at: 710 2689 4159 5027 5269 5350 5685 7829 710
Size: 1979 1470 868 242 81 335 2144 938
Fragments arranged by size:
2144 1979 1470 938 868 335 242 81
BmtI G_CTAG'C
Cuts at: 1364 1390 1364
Size: 26 8031
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1022 1953 6424 1022
Size: 931 4471 2655
Fragments arranged by size:
4471 2655 931
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3540 4592 4684 3540
Size: 1052 92 6913
Fragments arranged by size:
6913 1052 92
Bpu10I CC'TnA_GC
Cuts at: 989 4374 989
Size: 3385 4672
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 735 1520 2592 4138 5233 6112 6604 6902 735
Size: 785 1072 1546 1095 879 492 298 1890
Fragments arranged by size:
1890 1546 1095 1072 879 785 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 7761 7761
Size: 8057
BsaAI yAC'GTr
Cuts at: 5410 5410
Size: 8057
BsaBI GATnn'nnATC
Cuts at: 6 656 4691 5571 7608 6
Size: 650 4035 880 2037 455
Fragments arranged by size:
4035 2037 880 650 455
BsaHI Gr'CG_yC
Cuts at: 5488 5736 6438 7934 5488
Size: 248 702 1496 5611
Fragments arranged by size:
5611 1496 702 248
BsaWI w'CCGG_w
Cuts at: 5246 5767 6445 6719 6866 7277 5246
Size: 521 678 274 147 411 6026
Fragments arranged by size:
6026 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3666 3696 3666
Size: 30 8027
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 396 3687 4831 396
Size: 3291 1144 3622
Fragments arranged by size:
3622 3291 1144
BseYI C'CCAG_C
Cuts at: 1002 5004 5482 6240 6817 7313 1002
Size: 4002 478 758 577 496 1746
Fragments arranged by size:
4002 1746 758 577 496 478
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 999 2006 3370 4649 999
Size: 1007 1364 1279 4407
Fragments arranged by size:
4407 1364 1279 1007
BsiEI CG_ry'CG
Cuts at: 104 5599 5645 6853 104
Size: 5495 46 1208 1308
Fragments arranged by size:
5495 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3367 4608 4991 5017 5071 5849 6039 6831 3367
Size: 1241 383 26 54 778 190 792 4593
Fragments arranged by size:
4593 1241 792 778 383 190 54 26
BsmI GAATG_Cn'
Cuts at: 195 3750 4230 5029 195
Size: 3555 480 799 3223
Fragments arranged by size:
3555 3223 799 480
Bsp1286I G_dGCh'C
Cuts at: 1003 3367 4608 4991 5017 5071 5682 5775 5849
Size: 2364 1241 383 26 54 611 93 74
Cuts at: 5849 6039 6101 6831 1003
Size: 190 62 730 2229
Fragments arranged by size:
2364 2229 1241 730 611 383 190 93
74 62 54 26
BspEI T'CCGG_A
Cuts at: 7277 7277
Size: 8057
BspHI T'CATG_A
Cuts at: 1253 3960 7233 1253
Size: 2707 3273 2077
Fragments arranged by size:
3273 2707 2077
BspMI ACCTGCnnnn'nnnn_
Cuts at: 268 5107 5321 5623 6004 268
Size: 4839 214 302 381 2321
Fragments arranged by size:
4839 2321 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6349 6403 7887 8052 50
Size: 6299 54 1484 165 55
Fragments arranged by size:
6299 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 767 2132 2216 3560 4629 5969 767
Size: 1365 84 1344 1069 1340 2855
Fragments arranged by size:
2855 1365 1344 1340 1069 84
BsrFI r'CCGG_y
Cuts at: 6055 6236 6445 6055
Size: 181 209 7667
Fragments arranged by size:
7667 209 181
BsrGI T'GTAC_A
Cuts at: 949 4239 4447 949
Size: 3290 208 4559
Fragments arranged by size:
4559 3290 208
BssSI C'ACGA_G
Cuts at: 6328 6686 6328
Size: 358 7699
BstAPI GCAn_nnn'nTGC
Cuts at: 2131 2275 5434 2131
Size: 144 3159 4754
Fragments arranged by size:
4754 3159 144
BstBI TT'CG_AA
Cuts at: 1415 6418 1415
Size: 5003 3054
BstEII G'GTnAC_C
Cuts at: 4461 4461
Size: 8057
BstYI r'GATC_y
Cuts at: 1 271 657 1090 1524 1914 2532 4501 5082
Size: 270 386 433 434 390 618 1969 581
Cuts at: 5082 5549 5572 5907 6153 7165 7251 7263 7609
Size: 467 23 335 246 1012 86 12 346
Cuts at: 7609 1
Size: 449
Fragments arranged by size:
1969 1012 618 581 467 449 434 433
390 386 346 335 270 246 86 23
12
BtgI C'CryG_G
Cuts at: 4487 4992 4487
Size: 505 7552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6007 6188 6351 6007
Size: 181 163 7713
Fragments arranged by size:
7713 181 163
BtsI GCAGTG_nn'
Cuts at: 1932 4450 5015 5300 1932
Size: 2518 565 285 4689
Fragments arranged by size:
4689 2518 565 285
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2392 2427 2392
Size: 35 8022
DraI TTT'AAA
Cuts at: 5056 5056
Size: 8057
DrdI GACnn_nn'nnGTC
Cuts at: 3727 5763 6621 3727
Size: 2036 858 5163
Fragments arranged by size:
5163 2036 858
EaeI y'GGCC_r
Cuts at: 4730 5642 5816 6207 6234 7579 4730
Size: 912 174 391 27 1345 5208
Fragments arranged by size:
5208 1345 912 391 174 27
EagI C'GGCC_G
Cuts at: 5642 5642
Size: 8057
EarI CTCTTCn'nnn_
Cuts at: 642 2838 3068 6080 6290 642
Size: 2196 230 3012 210 2409
Fragments arranged by size:
3012 2409 2196 230 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3232 6265 6575 6721 7867 3232
Size: 3033 310 146 1146 3422
Fragments arranged by size:
3422 3033 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3365 4989 5015 5069 3365
Size: 1624 26 54 6353
Fragments arranged by size:
6353 1624 54 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 809 1039 3540 3635 4408 4592 4684 4737
Size: 448 230 2501 95 773 184 92 53
Cuts at: 4737 4880 5882 6314 7061 7329 361
Size: 143 1002 432 747 268 1089
Fragments arranged by size:
2501 1089 1002 773 747 448 432 268
230 184 143 95 92 53
EcoO109I rG'GnC_Cy
Cuts at: 5196 5196
Size: 8057
EcoRI G'AATT_C
Cuts at: 3254 4342 4612 5061 3254
Size: 1088 270 449 6250
Fragments arranged by size:
6250 1088 449 270
EcoRV GAT'ATC
Cuts at: 843 1740 843
Size: 897 7160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6076 6076
Size: 8057
FspI TGC'GCA
Cuts at: 5838 5838
Size: 8057
HaeII r_GCGC'y
Cuts at: 1935 5491 5739 6761 7740 1935
Size: 3556 248 1022 979 2252
Fragments arranged by size:
3556 2252 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1662 1694 2289 2318 2321 2350 4183 4215 4488
Size: 32 595 29 3 29 1833 32 273
Cuts at: 4488 4520 4584 4616 5899 5929 5931 5961 1662
Size: 32 64 32 1283 30 2 30 3758
Fragments arranged by size:
3758 1833 1283 595 273 64 32 32
32 32 30 30 29 29 3 2
HincII GTy'rAC
Cuts at: 2214 3180 5096 2214
Size: 966 1916 5175
Fragments arranged by size:
5175 1916 966
HindIII A'AGCT_T
Cuts at: 3263 3263
Size: 8057
KasI G'GCGC_C
Cuts at: 5487 5735 5487
Size: 248 7809
KpnI G_GTAC'C
Cuts at: 5077 5077
Size: 8057
MluI A'CGCG_T
Cuts at: 1197 7490 1197
Size: 6293 1764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 212 407 818 2287 2568 6728 6912 212
Size: 195 411 1469 281 4160 184 1357
Fragments arranged by size:
4160 1469 1357 411 281 195 184
MscI TGG'CCA
Cuts at: 4732 5818 4732
Size: 1086 6971
MslI CAynn'nnrTG
Cuts at: 673 1579 1834 1952 6046 6173 673
Size: 906 255 118 4094 127 2557
Fragments arranged by size:
4094 2557 906 255 127 118
MspA1I CmG'CkG
Cuts at: 143 514 2149 5168 5482 5842 6855 7100 143
Size: 371 1635 3019 314 360 1013 245 1100
Fragments arranged by size:
3019 1635 1100 1013 371 360 314 245
NaeI GCC'GGC
Cuts at: 6238 6238
Size: 8057
NarI GG'CG_CC
Cuts at: 5488 5736 5488
Size: 248 7809
NcoI C'CATG_G
Cuts at: 4487 4992 4487
Size: 505 7552
NdeI CA'TA_TG
Cuts at: 1695 2715 3033 1695
Size: 1020 318 6719
Fragments arranged by size:
6719 1020 318
NgoMIV G'CCGG_C
Cuts at: 6236 6236
Size: 8057
NheI G'CTAG_C
Cuts at: 1360 1386 1360
Size: 26 8031
NspI r_CATG'y
Cuts at: 342 4043 5110 5610 6517 342
Size: 3701 1067 500 907 1882
Fragments arranged by size:
3701 1882 1067 907 500
PasI CC'CwG_GG
Cuts at: 4886 4886
Size: 8057
PciI A'CATG_T
Cuts at: 6513 6513
Size: 8057
PfoI T'CCnGG_A
Cuts at: 3822 4334 3822
Size: 512 7545
PmeI GTTT'AAAC
Cuts at: 5056 5056
Size: 8057
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7196 7228 7196
Size: 32 8025
PshAI GACnn'nnGTC
Cuts at: 1372 1372
Size: 8057
PsiI TTA'TAA
Cuts at: 1304 3932 4898 1304
Size: 2628 966 4463
Fragments arranged by size:
4463 2628 966
PstI C_TGCA'G
Cuts at: 279 498 1149 5104 5789 279
Size: 219 651 3955 685 2547
Fragments arranged by size:
3955 2547 685 651 219
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8057
PvuII CAG'CTG
Cuts at: 514 5482 5842 514
Size: 4968 360 2729
Fragments arranged by size:
4968 2729 360
RsrII CG'GwC_CG
Cuts at: 6252 6252
Size: 8057
SacI G_AGCT'C
Cuts at: 3367 4991 5017 5071 3367
Size: 1624 26 54 6353
Fragments arranged by size:
6353 1624 54 26
SalI G'TCGA_C
Cuts at: 5094 5094
Size: 8057
SapI GCTCTTCn'nnn_
Cuts at: 6080 6290 6080
Size: 210 7847
SbfI CC_TGCA'GG
Cuts at: 5104 5104
Size: 8057
ScaI AGT'ACT
Cuts at: 123 748 7574 123
Size: 625 6826 606
Fragments arranged by size:
6826 625 606
SfcI C'TryA_G
Cuts at: 33 275 359 494 1127 1145 2582 3310 3893
Size: 242 84 135 633 18 1437 728 583
Cuts at: 3893 5100 5785 6778 6969 33
Size: 1207 685 993 191 1121
Fragments arranged by size:
1437 1207 1121 993 728 685 633 583
242 191 135 84 18
SfoI GGC'GCC
Cuts at: 5489 5737 5489
Size: 248 7809
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8057
SmaI CCC'GGG
Cuts at: 5079 5079
Size: 8057
SmlI C'TyrA_G
Cuts at: 214 742 750 994 1535 2358 2607 4117 5212
Size: 528 8 244 541 823 249 1510 1095
Cuts at: 5212 6127 6619 6881 214
Size: 915 492 262 1390
Fragments arranged by size:
1510 1390 1095 915 823 541 528 492
262 249 244 8
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8057
SphI G_CATG'C
Cuts at: 5110 5610 5110
Size: 500 7557
SspI AAT'ATT
Cuts at: 2313 2313
Size: 8057
StyI C'CwwG_G
Cuts at: 3953 4487 4992 5191 5545 3953
Size: 534 505 199 354 6465
Fragments arranged by size:
6465 534 505 354 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 214 780 2095 5037 5046 5640 5773 6232 6441
Size: 566 1315 2942 9 594 133 459 209
Cuts at: 6441 214
Size: 1830
Fragments arranged by size:
2942 1830 1315 594 566 459 209 133
9
TatI w'GTAC_w
Cuts at: 121 746 949 2656 3300 4239 4447 4554 7572
Size: 625 203 1707 644 939 208 107 3018
Cuts at: 7572 121
Size: 606
Fragments arranged by size:
3018 1707 939 644 625 606 208 203
107
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7574 7606 7574
Size: 32 8025
Tth111I GACn'n_nGTC
Cuts at: 5854 5854
Size: 8057
XbaI T'CTAG_A
Cuts at: 3664 4302 5088 3664
Size: 638 786 6633
Fragments arranged by size:
6633 786 638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5552 5552
Size: 8057
XmaI C'CCGG_G
Cuts at: 5077 5077
Size: 8057
XmnI GAAnn'nnTTC
Cuts at: 1610 2075 3702 4718 1610
Size: 465 1627 1016 4949
Fragments arranged by size:
4949 1627 1016 465
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NspI PasI PciI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AloI ApaI AscI AvrII BaeI
BcgI BfrBI BglI BsiWI BsmBI BssHII BstXI BstZ17I
Bsu36I ClaI DraIII EcoNI FseI FspAI HpaI MfeI
NotI NruI NsiI PacI PflMI PmlI PpuMI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI