Restriction map for
pF1KA0030
(Circular) (Six-base) MAPSORT of: pF1KA0030.seq Check: 5012 from: 1 to: 5825
pF1KA0030 5825 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 977 2625 977
Size: 1648 4177
AatII G_ACGT'C
Cuts at: 2480 2480
Size: 5825
AccI GT'mk_AC
Cuts at: 728 2863 728
Size: 2135 3690
Acc65I G'GTAC_C
Cuts at: 2841 2841
Size: 5825
AclI AA'CG_TT
Cuts at: 5665 5665
Size: 5825
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2287 2635 2748 3650 4082 4435 5097 2287
Size: 348 113 902 432 353 662 3015
Fragments arranged by size:
3015 902 662 432 353 348 113
AfeI AGC'GCT
Cuts at: 2208 2208
Size: 5825
AflIII A'CryG_T
Cuts at: 770 2231 3177 4977 5258 770
Size: 1461 946 1800 281 1337
Fragments arranged by size:
1800 1461 1337 946 281
AhdI GACnn_n'nnGTC
Cuts at: 428 428
Size: 5825
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2467 2467
Size: 5825
AlwNI CAG_nnn'CTG
Cuts at: 3130 4568 3130
Size: 1438 4387
ApaI G_GGCC'C
Cuts at: 293 679 1833 2667 293
Size: 386 1154 834 3451
Fragments arranged by size:
3451 1154 834 386
ApaLI G'TGCA_C
Cuts at: 4663 4663
Size: 5825
ApoI r'AATT_y
Cuts at: 1870 2829 5178 5434 1870
Size: 959 2349 256 2261
Fragments arranged by size:
2349 2261 959 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5825
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5825
AvaI C'yCGr_G
Cuts at: 2845 2845
Size: 5825
AvrII C'CTAG_G
Cuts at: 276 276
Size: 5825
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1215 1248 1215
Size: 33 5792
BamHI G'GATC_C
Cuts at: 2850 2850
Size: 5825
BanI G'GyrC_C
Cuts at: 858 1793 2841 3255 3503 3538 858
Size: 935 1048 414 248 35 3145
Fragments arranged by size:
3145 1048 935 414 248 35
BanII G_rGCy'C
Cuts at: 275 293 323 512 679 1619 1833 2063 2219
Size: 18 30 189 167 940 214 230 156
Cuts at: 2219 2249 2667 2762 2839 3869 275
Size: 30 418 95 77 1030 2231
Fragments arranged by size:
2231 1030 940 418 230 214 189 167
156 95 77 30 30 18
BbeI G_GCGC'C
Cuts at: 3259 3507 3259
Size: 248 5577
BbsI GAAGACnn'nnnn_
Cuts at: 741 1580 2477 741
Size: 839 897 4089
Fragments arranged by size:
4089 897 839
BbvCI CC'TCA_GC
Cuts at: 997 997
Size: 5825
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 708 742 708
Size: 34 5791
BciVI GTATCCnnnnn_n'
Cuts at: 1243 3723 4779 5825 1243
Size: 2480 1056 1046 1243
Fragments arranged by size:
2480 1243 1056 1046
BclI T'GATC_A
Cuts at: 1496 3345 1496
Size: 1849 3976
BglI GCCn_nnn'nGGC
Cuts at: 379 379
Size: 5825
BglII A'GATC_T
Cuts at: 878 1308 1535 3340 5377 878
Size: 430 227 1805 2037 1326
Fragments arranged by size:
2037 1805 1326 430 227
BlpI GC'TnA_GC
Cuts at: 1072 2213 2937 1072
Size: 1141 724 3960
Fragments arranged by size:
3960 1141 724
Bme1580I G_kGCm'C
Cuts at: 293 679 1833 2667 3450 3543 4667 293
Size: 386 1154 834 783 93 1124 1451
Fragments arranged by size:
1451 1154 1124 834 783 386 93
BmgBI CAC'GTC
Cuts at: 726 2157 726
Size: 1431 4394
BmrI ACTGGGnnnn_n'
Cuts at: 2107 3037 3118 3453 5597 2107
Size: 930 81 335 2144 2335
Fragments arranged by size:
2335 2144 930 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 337 1008 1876 4192 337
Size: 671 868 2316 1970
Fragments arranged by size:
2316 1970 868 671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 210 316 649 706 919 2524 168
Size: 42 106 333 57 213 1605 3469
Fragments arranged by size:
3469 1605 333 213 106 57 42
Bpu10I CC'TnA_GC
Cuts at: 997 997
Size: 5825
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1690 3001 3880 4594 4892 1690
Size: 1311 879 714 298 2623
Fragments arranged by size:
2623 1311 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1201 1670 5529 1201
Size: 469 3859 1497
Fragments arranged by size:
3859 1497 469
BsaAI yAC'GTr
Cuts at: 834 1437 1648 2234 3178 834
Size: 603 211 586 944 3481
Fragments arranged by size:
3481 944 603 586 211
BsaBI GATnn'nnATC
Cuts at: 6 2725 3339 5376 6
Size: 2719 614 2037 455
Fragments arranged by size:
2719 2037 614 455
BsaHI Gr'CG_yC
Cuts at: 371 1058 1292 1759 2477 3256 3504 4206 5702
Size: 687 234 467 718 779 248 702 1496
Cuts at: 5702 371
Size: 494
Fragments arranged by size:
1496 779 718 702 687 494 467 248
234
BsaWI w'CCGG_w
Cuts at: 3014 3535 4213 4624 4771 5045 3014
Size: 521 678 411 147 274 3794
Fragments arranged by size:
3794 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 331 430 544 553 607 616 997 1219 1291
Size: 99 114 9 54 9 381 222 72
Cuts at: 1291 1865 2197 2200 331
Size: 574 332 3 3956
Fragments arranged by size:
3956 574 381 332 222 114 99 72
54 9 9 3
BseYI C'CCAG_C
Cuts at: 152 1067 1825 2170 2666 3250 4008 4673 5081
Size: 915 758 345 496 584 758 665 408
Cuts at: 5081 152
Size: 896
Fragments arranged by size:
915 896 758 758 665 584 496 408
345
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1035 1962 1035
Size: 927 4898
BsiEI CG_ry'CG
Cuts at: 104 933 1181 3367 3413 4643 104
Size: 829 248 2186 46 1230 1286
Fragments arranged by size:
2186 1286 1230 829 248 46
BsiHKAI G_wGCw'C
Cuts at: 323 833 1667 2762 2839 3617 3807 4667 323
Size: 510 834 1095 77 778 190 860 1481
Fragments arranged by size:
1481 1095 860 834 778 510 190 77
BsmBI CGTCTCn'nnnn_
Cuts at: 1328 1328
Size: 5825
Bsp1286I G_dGCh'C
Cuts at: 275 293 323 512 679 833 1619 1667 1833
Size: 18 30 189 167 154 786 48 166
Cuts at: 1833 2063 2219 2249 2667 2762 2839 3450 3543
Size: 230 156 30 418 95 77 611 93
Cuts at: 3543 3617 3807 3869 4667 275
Size: 74 190 62 798 1433
Fragments arranged by size:
1433 798 786 611 418 230 190 189
167 166 156 154 95 93 77 74
62 48 30 30 18
BspHI T'CATG_A
Cuts at: 1569 4257 1569
Size: 2688 3137
BspMI ACCTGCnnnn'nnnn_
Cuts at: 977 2625 2875 3089 3391 3772 977
Size: 1648 250 214 302 381 3030
Fragments arranged by size:
3030 1648 381 302 250 214
BsrBI CCG'CTC
Cuts at: 50 450 573 1283 4117 4171 5655 5820 50
Size: 400 123 710 2834 54 1484 165 55
Fragments arranged by size:
2834 1484 710 400 165 123 55 54
BsrDI GCAATG_nn'
Cuts at: 174 1547 1985 3737 174
Size: 1373 438 1752 2262
Fragments arranged by size:
2262 1752 1373 438
BsrFI r'CCGG_y
Cuts at: 861 1180 1271 3823 4004 861
Size: 319 91 2552 181 2682
Fragments arranged by size:
2682 2552 319 181 91
BsrGI T'GTAC_A
Cuts at: 2345 2345
Size: 5825
BssHII G'CGCG_C
Cuts at: 2440 2440
Size: 5825
BssSI C'ACGA_G
Cuts at: 4096 4804 4096
Size: 708 5117
BstAPI GCAn_nnn'nTGC
Cuts at: 1100 3202 1100
Size: 2102 3723
BstBI TT'CG_AA
Cuts at: 4186 4186
Size: 5825
BstEII G'GTnAC_C
Cuts at: 1041 1041
Size: 5825
BstXI CCAn_nnnn'nTGG
Cuts at: 1038 1437 1038
Size: 399 5426
BstYI r'GATC_y
Cuts at: 1 633 689 878 1308 1535 2850 3317 3340
Size: 632 56 189 430 227 1315 467 23
Cuts at: 3340 3675 3921 4227 4239 4325 5377 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1315 1052 632 467 449 430 335 306
246 227 189 86 56 23 12
Bsu36I CC'TnA_GG
Cuts at: 854 2356 854
Size: 1502 4323
BtgI C'CryG_G
Cuts at: 107 134 504 578 735 1916 2105 107
Size: 27 370 74 157 1181 189 3827
Fragments arranged by size:
3827 1181 370 189 157 74 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 539 3775 3956 4119 539
Size: 3236 181 163 2245
Fragments arranged by size:
3236 2245 181 163
BtsI GCAGTG_nn'
Cuts at: 1388 3068 1388
Size: 1680 4145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1629 1664 1629
Size: 35 5790
DraI TTT'AAA
Cuts at: 2824 2824
Size: 5825
DraIII CAC_nnn'GTG
Cuts at: 651 651
Size: 5825
DrdI GACnn_nn'nnGTC
Cuts at: 3531 4875 3531
Size: 1344 4481
EaeI y'GGCC_r
Cuts at: 192 234 425 480 738 911 1178 1269 1439
Size: 42 191 55 258 173 267 91 170
Cuts at: 1439 1749 2195 2336 2486 3410 3584 3975 4002
Size: 310 446 141 150 924 174 391 27
Cuts at: 4002 5347 192
Size: 1345 670
Fragments arranged by size:
1345 924 670 446 391 310 267 258
191 174 173 170 150 141 91 55
42 27
EagI C'GGCC_G
Cuts at: 1178 3410 1178
Size: 2232 3593
EarI CTCTTCn'nnn_
Cuts at: 299 2611 2767 3848 4058 299
Size: 2312 156 1081 210 2066
Fragments arranged by size:
2312 2066 1081 210 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 880 2443 4033 4775 4921 5635 127
Size: 753 1563 1590 742 146 714 317
Fragments arranged by size:
1590 1563 753 742 714 317 146
EcoICRI GAG'CTC
Cuts at: 321 2760 2837 321
Size: 2439 77 3309
Fragments arranged by size:
3309 2439 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 210 316 649 706 919 2287 2524 2635
Size: 42 106 333 57 213 1368 237 111
Cuts at: 2635 2748 3650 4082 4435 5097 168
Size: 113 902 432 353 662 896
Fragments arranged by size:
1368 902 896 662 432 353 333 237
213 113 111 106 57 42
EcoNI CCTnn'n_nnAGG
Cuts at: 853 1510 2254 853
Size: 657 744 4424
Fragments arranged by size:
4424 744 657
EcoO109I rG'GnC_Cy
Cuts at: 289 290 486 676 1117 1829 1830 2262 2663
Size: 1 196 190 441 712 1 432 401
Cuts at: 2663 2685 2964 289
Size: 22 279 3150
Fragments arranged by size:
3150 712 441 432 401 279 196 190
22 1 1
EcoRI G'AATT_C
Cuts at: 2829 2829
Size: 5825
EcoRV GAT'ATC
Cuts at: 1656 1656
Size: 5825
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3844 3844
Size: 5825
FseI GG_CCGG'CC
Cuts at: 1184 1184
Size: 5825
FspI TGC'GCA
Cuts at: 718 2542 2551 3606 718
Size: 1824 9 1055 2937
Fragments arranged by size:
2937 1824 1055 9
HaeII r_GCGC'y
Cuts at: 546 561 2016 2210 3259 3507 4737 5508 546
Size: 15 1455 194 1049 248 1230 771 863
Fragments arranged by size:
1455 1230 1049 863 771 248 194 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 247 279 414 446 1159 1191 1200 1232 1520
Size: 32 135 32 713 32 9 32 288
Cuts at: 1520 1552 1708 1740 3667 3697 3699 3729 247
Size: 32 156 32 1927 30 2 30 2343
Fragments arranged by size:
2343 1927 713 288 156 135 32 32
32 32 32 32 30 30 9 2
HincII GTy'rAC
Cuts at: 729 1197 2481 2864 729
Size: 468 1284 383 3690
Fragments arranged by size:
3690 1284 468 383
KasI G'GCGC_C
Cuts at: 3255 3503 3255
Size: 248 5577
KpnI G_GTAC'C
Cuts at: 2845 2845
Size: 5825
MluI A'CGCG_T
Cuts at: 5258 5258
Size: 5825
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4584 4768 4584
Size: 184 5641
MscI TGG'CCA
Cuts at: 913 1441 1751 2197 2338 2488 3586 913
Size: 528 310 446 141 150 1098 3152
Fragments arranged by size:
3152 1098 528 446 310 150 141
MslI CAynn'nnrTG
Cuts at: 950 2395 2494 3814 3941 950
Size: 1445 99 1320 127 2834
Fragments arranged by size:
2834 1445 1320 127 99
MspA1I CmG'CkG
Cuts at: 1005 1020 1049 1322 1791 2936 3250 3610 4394
Size: 15 29 273 469 1145 314 360 784
Cuts at: 4394 4639 1005
Size: 245 2191
Fragments arranged by size:
2191 1145 784 469 360 314 273 245
29 15
NaeI GCC'GGC
Cuts at: 1182 1273 4006 1182
Size: 91 2733 3001
Fragments arranged by size:
3001 2733 91
NarI GG'CG_CC
Cuts at: 3256 3504 3256
Size: 248 5577
NcoI C'CATG_G
Cuts at: 107 134 1916 107
Size: 27 1782 4016
Fragments arranged by size:
4016 1782 27
NgoMIV G'CCGG_C
Cuts at: 1180 1271 4004 1180
Size: 91 2733 3001
Fragments arranged by size:
3001 2733 91
NspI r_CATG'y
Cuts at: 501 774 2550 2878 3378 4981 501
Size: 273 1776 328 500 1603 1345
Fragments arranged by size:
1776 1603 1345 500 328 273
PciI A'CATG_T
Cuts at: 770 4977 770
Size: 4207 1618
PfoI T'CCnGG_A
Cuts at: 1241 2117 1241
Size: 876 4949
PmeI GTTT'AAAC
Cuts at: 2824 2824
Size: 5825
PmlI CAC'GTG
Cuts at: 834 1437 834
Size: 603 5222
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4271 4303 4271
Size: 32 5793
PpuMI rG'GwC_Cy
Cuts at: 1117 2262 2685 1117
Size: 1145 423 4257
Fragments arranged by size:
4257 1145 423
PspOMI G'GGCC_C
Cuts at: 289 675 1829 2663 289
Size: 386 1154 834 3451
Fragments arranged by size:
3451 1154 834 386
PstI C_TGCA'G
Cuts at: 878 1970 2813 2872 3557 878
Size: 1092 843 59 685 3146
Fragments arranged by size:
3146 1092 843 685 59
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5825
PvuII CAG'CTG
Cuts at: 1005 1020 1049 1322 3250 3610 1005
Size: 15 29 273 1928 360 3220
Fragments arranged by size:
3220 1928 360 273 29 15
RsrII CG'GwC_CG
Cuts at: 4020 4020
Size: 5825
SacI G_AGCT'C
Cuts at: 323 2762 2839 323
Size: 2439 77 3309
Fragments arranged by size:
3309 2439 77
SalI G'TCGA_C
Cuts at: 727 2862 727
Size: 2135 3690
SapI GCTCTTCn'nnn_
Cuts at: 2611 2767 3848 4058 2611
Size: 156 1081 210 4378
Fragments arranged by size:
4378 1081 210 156
SbfI CC_TGCA'GG
Cuts at: 1970 2872 1970
Size: 902 4923
ScaI AGT'ACT
Cuts at: 5342 5342
Size: 5825
SfcI C'TryA_G
Cuts at: 33 874 1463 1966 2809 2868 3553 4521 4712
Size: 841 589 503 843 59 685 968 191
Cuts at: 4712 33
Size: 1146
Fragments arranged by size:
1146 968 843 841 685 589 503 191
59
SfoI GGC'GCC
Cuts at: 3257 3505 3257
Size: 248 5577
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5825
SgrAI Cr'CCGG_yG
Cuts at: 861 861
Size: 5825
SmaI CCC'GGG
Cuts at: 2847 2847
Size: 5825
SmlI C'TyrA_G
Cuts at: 1705 2980 3895 4609 4871 1705
Size: 1275 915 714 262 2659
Fragments arranged by size:
2659 1275 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5825
SphI G_CATG'C
Cuts at: 501 2550 2878 3378 501
Size: 2049 328 500 2948
Fragments arranged by size:
2948 2049 500 328
StuI AGG'CCT
Cuts at: 1589 1589
Size: 5825
StyI C'CwwG_G
Cuts at: 107 134 276 1286 1511 1817 1916 2288 2959
Size: 27 142 1010 225 306 99 372 671
Cuts at: 2959 3313 107
Size: 354 2619
Fragments arranged by size:
2619 1010 671 372 354 306 225 142
99 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 651 1492 1862 3408 3541 4000 4209 651
Size: 841 370 1546 133 459 209 2267
Fragments arranged by size:
2267 1546 841 459 370 209 133
TatI w'GTAC_w
Cuts at: 1085 2273 2345 5340 1085
Size: 1188 72 2995 1570
Fragments arranged by size:
2995 1570 1188 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2395 2427 5342 5374 2395
Size: 32 2915 32 2846
Fragments arranged by size:
2915 2846 32 32
Tth111I GACn'n_nGTC
Cuts at: 2113 2476 3622 2113
Size: 363 1146 4316
Fragments arranged by size:
4316 1146 363
XbaI T'CTAG_A
Cuts at: 2856 2856
Size: 5825
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 363 2421 3320 363
Size: 2058 899 2868
Fragments arranged by size:
2868 2058 899
XmaI C'CCGG_G
Cuts at: 2845 2845
Size: 5825
XmnI GAAnn'nnTTC
Cuts at: 2776 2776
Size: 5825
ZraI GAC'GTC
Cuts at: 2478 2478
Size: 5825
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AleI AloI AscI BfrBI BmtI BsaXI
BsiWI BsmI BspEI BstZ17I ClaI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SnaBI SrfI SspI SwaI XhoI