Restriction map for
pF1KA0029
(Circular) (Six-base) MAPSORT of: pF1KA0029.seq Check: 4094 from: 1 to: 6026
pF1KA0029 6026 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 2339 3064 2339
Size: 725 5301
Acc65I G'GTAC_C
Cuts at: 3042 3042
Size: 6026
AclI AA'CG_TT
Cuts at: 732 5866 732
Size: 5134 892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 968 1104 1432 1892 2066 2950 3851 4283 5030
Size: 136 328 460 174 884 901 432 747
Cuts at: 5030 5298 968
Size: 268 1696
Fragments arranged by size:
1696 901 884 747 460 432 328 268
174 136
AflIII A'CryG_T
Cuts at: 3378 4482 5459 3378
Size: 1104 977 3945
Fragments arranged by size:
3945 1104 977
AgeI A'CCGG_T
Cuts at: 4414 4414
Size: 6026
AhdI GACnn_n'nnGTC
Cuts at: 2485 2787 2485
Size: 302 5724
AlwNI CAG_nnn'CTG
Cuts at: 2532 3331 4898 2532
Size: 799 1567 3660
Fragments arranged by size:
3660 1567 799
ApaLI G'TGCA_C
Cuts at: 4796 4796
Size: 6026
ApoI r'AATT_y
Cuts at: 284 439 506 547 685 1952 2326 3030 5379
Size: 155 67 41 138 1267 374 704 2349
Cuts at: 5379 5635 284
Size: 256 675
Fragments arranged by size:
2349 1267 704 675 374 256 155 138
67 41
AseI AT'TA_AT
Cuts at: 20 20
Size: 6026
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6026
AvaI C'yCGr_G
Cuts at: 2491 3046 2491
Size: 555 5471
AvrII C'CTAG_G
Cuts at: 1353 1353
Size: 6026
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1681 1714 1681
Size: 33 5993
BamHI G'GATC_C
Cuts at: 3051 3051
Size: 6026
BanI G'GyrC_C
Cuts at: 953 2758 3042 3456 3704 3739 953
Size: 1805 284 414 248 35 3240
Fragments arranged by size:
3240 1805 414 284 248 35
BanII G_rGCy'C
Cuts at: 3040 4070 3040
Size: 1030 4996
BbeI G_GCGC'C
Cuts at: 957 2762 3460 3708 957
Size: 1805 698 248 3275
Fragments arranged by size:
3275 1805 698 248
BbsI GAAGACnn'nnnn_
Cuts at: 725 725
Size: 6026
BciVI GTATCCnnnnn_n'
Cuts at: 2522 3924 4685 6026 2522
Size: 1402 761 1341 2522
Fragments arranged by size:
2522 1402 1341 761
BclI T'GATC_A
Cuts at: 624 678 2298 3546 624
Size: 54 1620 1248 3104
Fragments arranged by size:
3104 1620 1248 54
BglII A'GATC_T
Cuts at: 429 991 3541 5578 429
Size: 562 2550 2037 877
Fragments arranged by size:
2550 2037 877 562
BlpI GC'TnA_GC
Cuts at: 2411 3138 2411
Size: 727 5299
Bme1580I G_kGCm'C
Cuts at: 2259 3651 3744 4800 2259
Size: 1392 93 1056 3485
Fragments arranged by size:
3485 1392 1056 93
BmgBI CAC'GTC
Cuts at: 2800 2800
Size: 6026
BmrI ACTGGGnnnn_n'
Cuts at: 1699 2781 2855 3238 3319 3654 5798 1699
Size: 1082 74 383 81 335 2144 1927
Fragments arranged by size:
2144 1927 1082 383 335 81 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 345 1400 4393 345
Size: 1055 2993 1978
Fragments arranged by size:
2993 1978 1055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1074 1594 1652 1668 1074
Size: 520 58 16 5432
Fragments arranged by size:
5432 520 58 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 727 1922 1931 3202 4081 4573 4871 727
Size: 1195 9 1271 879 492 298 1882
Fragments arranged by size:
1882 1271 1195 879 492 298 9
BsaI GGTCTCn'nnnn_
Cuts at: 851 1364 5730 851
Size: 513 4366 1147
Fragments arranged by size:
4366 1147 513
BsaAI yAC'GTr
Cuts at: 541 3379 541
Size: 2838 3188
BsaBI GATnn'nnATC
Cuts at: 6 3540 5577 6
Size: 3534 2037 455
Fragments arranged by size:
3534 2037 455
BsaHI Gr'CG_yC
Cuts at: 954 2759 3457 3705 4407 5903 954
Size: 1805 698 248 702 1496 1077
Fragments arranged by size:
1805 1496 1077 702 698 248
BsaWI w'CCGG_w
Cuts at: 2768 3215 3736 4414 4688 4835 5246 2768
Size: 447 521 678 274 147 411 3548
Fragments arranged by size:
3548 678 521 447 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1666 1696 2651 2681 2857 2887 1666
Size: 30 955 30 176 30 4805
Fragments arranged by size:
4805 955 176 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1493 1517 1520 1523 1493
Size: 24 3 3 5996
Fragments arranged by size:
5996 24 3 3
BseYI C'CCAG_C
Cuts at: 944 2153 3451 4209 4786 5282 944
Size: 1209 1298 758 577 496 1688
Fragments arranged by size:
1688 1298 1209 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2542 2900 2542
Size: 358 5668
BsiEI CG_ry'CG
Cuts at: 104 3568 3614 4822 104
Size: 3464 46 1208 1308
Fragments arranged by size:
3464 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1610 3040 3818 4008 4800 1610
Size: 1430 778 190 792 2836
Fragments arranged by size:
2836 1430 792 778 190
BsmI GAATG_Cn'
Cuts at: 2033 2928 2033
Size: 895 5131
Bsp1286I G_dGCh'C
Cuts at: 1610 2259 3040 3651 3744 3818 4008 4070 4800
Size: 649 781 611 93 74 190 62 730
Cuts at: 4800 1610
Size: 2836
Fragments arranged by size:
2836 781 730 649 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 5246 5246
Size: 6026
BspHI T'CATG_A
Cuts at: 1475 5202 1475
Size: 3727 2299
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1104 1764 2102 3076 3290 3592 3973 1104
Size: 660 338 974 214 302 381 3157
Fragments arranged by size:
3157 974 660 381 338 302 214
BsrBI CCG'CTC
Cuts at: 50 4318 4372 5856 6021 50
Size: 4268 54 1484 165 55
Fragments arranged by size:
4268 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3938 3938
Size: 6026
BsrFI r'CCGG_y
Cuts at: 4024 4205 4414 4024
Size: 181 209 5636
Fragments arranged by size:
5636 209 181
BsrGI T'GTAC_A
Cuts at: 2176 2176
Size: 6026
BssSI C'ACGA_G
Cuts at: 4297 4655 4297
Size: 358 5668
BstAPI GCAn_nnn'nTGC
Cuts at: 2923 3403 2923
Size: 480 5546
BstBI TT'CG_AA
Cuts at: 1083 4387 1083
Size: 3304 2722
BstEII G'GTnAC_C
Cuts at: 2089 2089
Size: 6026
BstXI CCAn_nnnn'nTGG
Cuts at: 1052 1276 2868 1052
Size: 224 1592 4210
Fragments arranged by size:
4210 1592 224
BstYI r'GATC_y
Cuts at: 1 290 429 991 1264 2008 2765 3051 3518
Size: 289 139 562 273 744 757 286 467
Cuts at: 3518 3541 3876 4122 5134 5220 5232 5578 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 757 744 562 467 449 346 335
289 286 273 246 139 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3976 4157 4320 3976
Size: 181 163 5682
Fragments arranged by size:
5682 181 163
BtsI GCAGTG_nn'
Cuts at: 1744 1930 2278 2340 2530 2829 2834 3269 1744
Size: 186 348 62 190 299 5 435 4501
Fragments arranged by size:
4501 435 348 299 190 186 62 5
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2631 2666 2631
Size: 35 5991
DraI TTT'AAA
Cuts at: 2751 3025 2751
Size: 274 5752
DraIII CAC_nnn'GTG
Cuts at: 2203 2203
Size: 6026
DrdI GACnn_nn'nnGTC
Cuts at: 2347 3732 4590 2347
Size: 1385 858 3783
Fragments arranged by size:
3783 1385 858
EaeI y'GGCC_r
Cuts at: 1854 2523 3611 3785 4176 4203 5548 1854
Size: 669 1088 174 391 27 1345 2332
Fragments arranged by size:
2332 1345 1088 669 391 174 27
EagI C'GGCC_G
Cuts at: 3611 3611
Size: 6026
EarI CTCTTCn'nnn_
Cuts at: 314 833 1085 2085 4049 4259 314
Size: 519 252 1000 1964 210 2081
Fragments arranged by size:
2081 1964 1000 519 252 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4234 4544 4690 5836 4234
Size: 310 146 1146 4424
Fragments arranged by size:
4424 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3038 3038
Size: 6026
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 968 1074 1104 1432 1594 1652 1668 1892 2066
Size: 106 30 328 162 58 16 224 174
Cuts at: 2066 2950 3851 4283 5030 5298 968
Size: 884 901 432 747 268 1696
Fragments arranged by size:
1696 901 884 747 432 328 268 224
174 162 106 58 30 16
EcoO109I rG'GnC_Cy
Cuts at: 2781 3165 2781
Size: 384 5642
EcoRI G'AATT_C
Cuts at: 1952 3030 1952
Size: 1078 4948
EcoRV GAT'ATC
Cuts at: 1573 1573
Size: 6026
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4045 4045
Size: 6026
FspI TGC'GCA
Cuts at: 1760 3807 1760
Size: 2047 3979
HaeII r_GCGC'y
Cuts at: 957 2762 3460 3708 4730 5709 957
Size: 1805 698 248 1022 979 1274
Fragments arranged by size:
1805 1274 1022 979 698 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1758 1790 1880 1912 2471 2503 2773 2805 2857
Size: 32 90 32 559 32 270 32 52
Cuts at: 2857 2889 3868 3898 3900 3930 1758
Size: 32 979 30 2 30 3854
Fragments arranged by size:
3854 979 559 270 90 52 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 299 2062 2952 3065 299
Size: 1763 890 113 3260
Fragments arranged by size:
3260 1763 890 113
HindIII A'AGCT_T
Cuts at: 2731 2731
Size: 6026
HpaI GTT'AAC
Cuts at: 299 2952 299
Size: 2653 3373
KasI G'GCGC_C
Cuts at: 953 2758 3456 3704 953
Size: 1805 698 248 3275
Fragments arranged by size:
3275 1805 698 248
KpnI G_GTAC'C
Cuts at: 3046 3046
Size: 6026
MluI A'CGCG_T
Cuts at: 5459 5459
Size: 6026
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1797 4697 4881 1797
Size: 2900 184 2942
Fragments arranged by size:
2942 2900 184
MscI TGG'CCA
Cuts at: 1856 2525 3787 1856
Size: 669 1262 4095
Fragments arranged by size:
4095 1262 669
MslI CAynn'nnrTG
Cuts at: 112 4015 4142 112
Size: 3903 127 1996
Fragments arranged by size:
3903 1996 127
MspA1I CmG'CkG
Cuts at: 2182 2415 2914 3137 3451 3811 4824 5069 2182
Size: 233 499 223 314 360 1013 245 3139
Fragments arranged by size:
3139 1013 499 360 314 245 233 223
NaeI GCC'GGC
Cuts at: 4207 4207
Size: 6026
NarI GG'CG_CC
Cuts at: 954 2759 3457 3705 954
Size: 1805 698 248 3275
Fragments arranged by size:
3275 1805 698 248
NgoMIV G'CCGG_C
Cuts at: 4205 4205
Size: 6026
NspI r_CATG'y
Cuts at: 3079 3579 4486 3079
Size: 500 907 4619
Fragments arranged by size:
4619 907 500
PasI CC'CwG_GG
Cuts at: 470 470
Size: 6026
PciI A'CATG_T
Cuts at: 4482 4482
Size: 6026
PflMI CCAn_nnn'nTGG
Cuts at: 2203 2521 2203
Size: 318 5708
PfoI T'CCnGG_A
Cuts at: 2103 2777 2103
Size: 674 5352
PmeI GTTT'AAAC
Cuts at: 3025 3025
Size: 6026
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5165 5197 5165
Size: 32 5994
PpuMI rG'GwC_Cy
Cuts at: 2781 2781
Size: 6026
PstI C_TGCA'G
Cuts at: 2537 3073 3758 2537
Size: 536 685 4805
Fragments arranged by size:
4805 685 536
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6026
PvuII CAG'CTG
Cuts at: 2182 2415 2914 3451 3811 2182
Size: 233 499 537 360 4397
Fragments arranged by size:
4397 537 499 360 233
RsrII CG'GwC_CG
Cuts at: 4221 4221
Size: 6026
SacI G_AGCT'C
Cuts at: 3040 3040
Size: 6026
SalI G'TCGA_C
Cuts at: 3063 3063
Size: 6026
SanDI GG'GwC_CC
Cuts at: 2781 2781
Size: 6026
SapI GCTCTTCn'nnn_
Cuts at: 2085 4049 4259 2085
Size: 1964 210 3852
Fragments arranged by size:
3852 1964 210
SbfI CC_TGCA'GG
Cuts at: 3073 3073
Size: 6026
ScaI AGT'ACT
Cuts at: 1036 5543 1036
Size: 4507 1519
SexAI A'CCwGG_T
Cuts at: 779 2055 2199 779
Size: 1276 144 4606
Fragments arranged by size:
4606 1276 144
SfcI C'TryA_G
Cuts at: 33 821 2040 2533 3069 3754 4747 4938 33
Size: 788 1219 493 536 685 993 191 1121
Fragments arranged by size:
1219 1121 993 788 685 536 493 191
SfoI GGC'GCC
Cuts at: 955 2760 3458 3706 955
Size: 1805 698 248 3275
Fragments arranged by size:
3275 1805 698 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6026
SmaI CCC'GGG
Cuts at: 2493 3048 2493
Size: 555 5471
SmlI C'TyrA_G
Cuts at: 742 1937 1946 3181 4096 4588 4850 742
Size: 1195 9 1235 915 492 262 1918
Fragments arranged by size:
1918 1235 1195 915 492 262 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6026
SphI G_CATG'C
Cuts at: 3079 3579 3079
Size: 500 5526
SspI AAT'ATT
Cuts at: 866 2290 2346 866
Size: 1424 56 4546
Fragments arranged by size:
4546 1424 56
StyI C'CwwG_G
Cuts at: 1353 1806 2649 3160 3514 1353
Size: 453 843 511 354 3865
Fragments arranged by size:
3865 843 511 453 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 875 3609 3742 4201 4410 875
Size: 2734 133 459 209 2491
Fragments arranged by size:
2734 2491 459 209 133
TatI w'GTAC_w
Cuts at: 1034 2118 2176 2537 5541 1034
Size: 1084 58 361 3004 1519
Fragments arranged by size:
3004 1519 1084 361 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1666 1698 5543 5575 1666
Size: 32 3845 32 2117
Fragments arranged by size:
3845 2117 32 32
Tth111I GACn'n_nGTC
Cuts at: 3823 3823
Size: 6026
XbaI T'CTAG_A
Cuts at: 2476 3057 2476
Size: 581 5445
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1813 2179 3521 1813
Size: 366 1342 4318
Fragments arranged by size:
4318 1342 366
XmaI C'CCGG_G
Cuts at: 2491 3046 2491
Size: 555 5471
XmnI GAAnn'nnTTC
Cuts at: 722 867 2136 722
Size: 145 1269 4612
Fragments arranged by size:
4612 1269 145
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI
BsmBI BssHII BstZ17I Bsu36I BtgI ClaI EcoNI FseI
FspAI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI