Restriction map for
pF1KA0013
(Circular) (Six-base) MAPSORT of: pF1KA0013.seq Check: 4368 from: 1 to: 6182
pF1KA0013 6182 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1285 1794 1285
Size: 509 5673
AccI GT'mk_AC
Cuts at: 2938 3220 2938
Size: 282 5900
Acc65I G'GTAC_C
Cuts at: 3198 3198
Size: 6182
AclI AA'CG_TT
Cuts at: 6022 6022
Size: 6182
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 4007 4439 4792 5454 703
Size: 3304 432 353 662 1431
Fragments arranged by size:
3304 1431 662 432 353
AflII C'TTAA_G
Cuts at: 469 2599 469
Size: 2130 4052
AflIII A'CryG_T
Cuts at: 2410 2476 3534 5334 5615 2410
Size: 66 1058 1800 281 2977
Fragments arranged by size:
2977 1800 1058 281 66
AgeI A'CCGG_T
Cuts at: 1203 1203
Size: 6182
AlwNI CAG_nnn'CTG
Cuts at: 123 150 379 2494 3487 4925 123
Size: 27 229 2115 993 1438 1380
Fragments arranged by size:
2115 1438 1380 993 229 27
ApaLI G'TGCA_C
Cuts at: 5020 5020
Size: 6182
ApoI r'AATT_y
Cuts at: 994 1420 2254 3186 5535 5791 994
Size: 426 834 932 2349 256 1385
Fragments arranged by size:
2349 1385 932 834 426 256
AseI AT'TA_AT
Cuts at: 20 2642 20
Size: 2622 3560
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6182
AvaI C'yCGr_G
Cuts at: 3202 3202
Size: 6182
AvrII C'CTAG_G
Cuts at: 2437 2437
Size: 6182
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1190 1223 3099 3132 1190
Size: 33 1876 33 4240
Fragments arranged by size:
4240 1876 33 33
BamHI G'GATC_C
Cuts at: 3207 3207
Size: 6182
BanI G'GyrC_C
Cuts at: 3198 3612 3860 3895 3198
Size: 414 248 35 5485
Fragments arranged by size:
5485 414 248 35
BanII G_rGCy'C
Cuts at: 500 3196 4226 500
Size: 2696 1030 2456
Fragments arranged by size:
2696 2456 1030
BbeI G_GCGC'C
Cuts at: 3616 3864 3616
Size: 248 5934
BbsI GAAGACnn'nnnn_
Cuts at: 344 583 740 960 344
Size: 239 157 220 5566
Fragments arranged by size:
5566 239 220 157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 181 215 181
Size: 34 6148
BciVI GTATCCnnnnn_n'
Cuts at: 4080 5136 6182 4080
Size: 1056 1046 4080
Fragments arranged by size:
4080 1056 1046
BclI T'GATC_A
Cuts at: 1286 2532 3702 1286
Size: 1246 1170 3766
Fragments arranged by size:
3766 1246 1170
BfrBI ATG'CAT
Cuts at: 811 811
Size: 6182
BglII A'GATC_T
Cuts at: 1405 2960 3697 5734 1405
Size: 1555 737 2037 1853
Fragments arranged by size:
2037 1853 1555 737
BlpI GC'TnA_GC
Cuts at: 1085 3294 1085
Size: 2209 3973
Bme1580I G_kGCm'C
Cuts at: 3807 3900 5024 3807
Size: 93 1124 4965
Fragments arranged by size:
4965 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 3394 3475 3810 5954 3394
Size: 81 335 2144 3622
Fragments arranged by size:
3622 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2895 4549 2895
Size: 1654 4528
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1616 2244 1616
Size: 628 5554
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1013 1107 1759 3358 4237 4951 5249 1013
Size: 94 652 1599 879 714 298 1946
Fragments arranged by size:
1946 1599 879 714 652 298 94
BsaI GGTCTCn'nnnn_
Cuts at: 883 3075 5886 883
Size: 2192 2811 1179
Fragments arranged by size:
2811 2192 1179
BsaAI yAC'GTr
Cuts at: 3535 3535
Size: 6182
BsaBI GATnn'nnATC
Cuts at: 6 3696 5733 6
Size: 3690 2037 455
Fragments arranged by size:
3690 2037 455
BsaHI Gr'CG_yC
Cuts at: 3613 3861 4563 6059 3613
Size: 248 702 1496 3736
Fragments arranged by size:
3736 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1203 1658 3371 3892 4570 4981 5128 5402 1203
Size: 455 1713 521 678 411 147 274 1983
Fragments arranged by size:
1983 1713 678 521 455 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 959 989 2694 2724 959
Size: 30 1705 30 4417
Fragments arranged by size:
4417 1705 30 30
BseYI C'CCAG_C
Cuts at: 507 1046 3607 4365 5030 5438 507
Size: 539 2561 758 665 408 1251
Fragments arranged by size:
2561 1251 758 665 539 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 2381 425
Size: 1956 4226
BsiEI CG_ry'CG
Cuts at: 104 201 3724 3770 5000 104
Size: 97 3523 46 1230 1286
Fragments arranged by size:
3523 1286 1230 97 46
BsiHKAI G_wGCw'C
Cuts at: 987 2745 3196 3974 4164 5024 987
Size: 1758 451 778 190 860 2145
Fragments arranged by size:
2145 1758 860 778 451 190
BsmI GAATG_Cn'
Cuts at: 2928 2928
Size: 6182
Bsp1286I G_dGCh'C
Cuts at: 500 987 2745 3196 3807 3900 3974 4164 4226
Size: 487 1758 451 611 93 74 190 62
Cuts at: 4226 5024 500
Size: 798 1658
Fragments arranged by size:
1758 1658 798 611 487 451 190 93
74 62
BspHI T'CATG_A
Cuts at: 3009 4614 3009
Size: 1605 4577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1285 1794 3232 3446 3748 4129 1285
Size: 509 1438 214 302 381 3338
Fragments arranged by size:
3338 1438 509 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4474 4528 6012 6177 50
Size: 4424 54 1484 165 55
Fragments arranged by size:
4424 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1093 4094 1093
Size: 3001 3181
BsrFI r'CCGG_y
Cuts at: 1203 4180 4361 1203
Size: 2977 181 3024
Fragments arranged by size:
3024 2977 181
BssSI C'ACGA_G
Cuts at: 4453 5161 4453
Size: 708 5474
BstAPI GCAn_nnn'nTGC
Cuts at: 150 3559 150
Size: 3409 2773
BstBI TT'CG_AA
Cuts at: 1401 4543 1401
Size: 3142 3040
BstYI r'GATC_y
Cuts at: 1 376 658 1405 2386 2960 3207 3674 3697
Size: 375 282 747 981 574 247 467 23
Cuts at: 3697 4032 4278 4584 4596 4682 5734 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 981 747 574 467 449 375 335
306 282 247 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3076 3076
Size: 6182
BtgI C'CryG_G
Cuts at: 186 224 186
Size: 38 6144
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4132 4313 4476 4132
Size: 181 163 5838
Fragments arranged by size:
5838 181 163
BtsI GCAGTG_nn'
Cuts at: 199 265 1289 3425 199
Size: 66 1024 2136 2956
Fragments arranged by size:
2956 2136 1024 66
ClaI AT'CG_AT
Cuts at: 803 1220 803
Size: 417 5765
DraI TTT'AAA
Cuts at: 999 1517 1820 2564 3181 999
Size: 518 303 744 617 4000
Fragments arranged by size:
4000 744 617 518 303
DrdI GACnn_nn'nnGTC
Cuts at: 3888 5232 3888
Size: 1344 4838
EaeI y'GGCC_r
Cuts at: 2973 3767 3941 4332 4359 5704 2973
Size: 794 174 391 27 1345 3451
Fragments arranged by size:
3451 1345 794 391 174 27
EagI C'GGCC_G
Cuts at: 3767 3767
Size: 6182
EarI CTCTTCn'nnn_
Cuts at: 1189 2828 4205 4415 1189
Size: 1639 1377 210 2956
Fragments arranged by size:
2956 1639 1377 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4390 5132 5278 5992 4390
Size: 742 146 714 4580
Fragments arranged by size:
4580 742 714 146
EcoICRI GAG'CTC
Cuts at: 3194 3194
Size: 6182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 703 1616 2244 4007 4439 4792 5454 703
Size: 913 628 1763 432 353 662 1431
Fragments arranged by size:
1763 1431 913 662 628 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1675 2803 3072 3101 3321 1675
Size: 1128 269 29 220 4536
Fragments arranged by size:
4536 1128 269 220 29
EcoRI G'AATT_C
Cuts at: 2254 3186 2254
Size: 932 5250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3006 4201 3006
Size: 1195 4987
FspI TGC'GCA
Cuts at: 3963 3963
Size: 6182
HaeII r_GCGC'y
Cuts at: 3616 3864 5094 5865 3616
Size: 248 1230 771 3933
Fragments arranged by size:
3933 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 796 828 1213 1245 2371 2403 4024 4054 4056
Size: 32 385 32 1126 32 1621 30 2
Cuts at: 4056 4086 796
Size: 30 2892
Fragments arranged by size:
2892 1621 1126 385 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3221 3221
Size: 6182
HindIII A'AGCT_T
Cuts at: 306 1715 2554 306
Size: 1409 839 3934
Fragments arranged by size:
3934 1409 839
KasI G'GCGC_C
Cuts at: 3612 3860 3612
Size: 248 5934
KpnI G_GTAC'C
Cuts at: 3202 3202
Size: 6182
MluI A'CGCG_T
Cuts at: 5615 5615
Size: 6182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1626 2633 4941 5125 1626
Size: 1007 2308 184 2683
Fragments arranged by size:
2683 2308 1007 184
MscI TGG'CCA
Cuts at: 2975 3943 2975
Size: 968 5214
MslI CAynn'nnrTG
Cuts at: 4171 4298 4171
Size: 127 6055
MspA1I CmG'CkG
Cuts at: 511 3293 3607 3967 4751 4996 511
Size: 2782 314 360 784 245 1697
Fragments arranged by size:
2782 1697 784 360 314 245
NaeI GCC'GGC
Cuts at: 4363 4363
Size: 6182
NarI GG'CG_CC
Cuts at: 3613 3861 3613
Size: 248 5934
NgoMIV G'CCGG_C
Cuts at: 4361 4361
Size: 6182
NsiI A_TGCA'T
Cuts at: 813 813
Size: 6182
NspI r_CATG'y
Cuts at: 2414 2480 3235 3735 5338 2414
Size: 66 755 500 1603 3258
Fragments arranged by size:
3258 1603 755 500 66
PciI A'CATG_T
Cuts at: 2410 2476 5334 2410
Size: 66 2858 3258
Fragments arranged by size:
3258 2858 66
PflMI CCAn_nnn'nTGG
Cuts at: 292 292
Size: 6182
PfoI T'CCnGG_A
Cuts at: 842 842
Size: 6182
PmeI GTTT'AAAC
Cuts at: 3181 3181
Size: 6182
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4628 4660 4628
Size: 32 6150
PpuMI rG'GwC_Cy
Cuts at: 1675 2803 3072 1675
Size: 1128 269 4785
Fragments arranged by size:
4785 1128 269
PsiI TTA'TAA
Cuts at: 2951 2951
Size: 6182
PstI C_TGCA'G
Cuts at: 786 1296 3229 3914 786
Size: 510 1933 685 3054
Fragments arranged by size:
3054 1933 685 510
PvuI CG_AT'CG
Cuts at: 104 201 104
Size: 97 6085
PvuII CAG'CTG
Cuts at: 511 3607 3967 511
Size: 3096 360 2726
Fragments arranged by size:
3096 2726 360
RsrII CG'GwC_CG
Cuts at: 4377 4377
Size: 6182
SacI G_AGCT'C
Cuts at: 3196 3196
Size: 6182
SalI G'TCGA_C
Cuts at: 3219 3219
Size: 6182
SapI GCTCTTCn'nnn_
Cuts at: 4205 4415 4205
Size: 210 5972
SbfI CC_TGCA'GG
Cuts at: 3229 3229
Size: 6182
ScaI AGT'ACT
Cuts at: 5699 5699
Size: 6182
SfcI C'TryA_G
Cuts at: 33 782 1292 2057 2672 2942 3167 3225 3910
Size: 749 510 765 615 270 225 58 685
Cuts at: 3910 4878 5069 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 765 749 685 615 510 270
225 191 58
SfoI GGC'GCC
Cuts at: 3614 3862 3614
Size: 248 5934
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6182
SmaI CCC'GGG
Cuts at: 3204 3204
Size: 6182
SmlI C'TyrA_G
Cuts at: 469 1028 1122 1738 2599 3337 4252 4966 5228
Size: 559 94 616 861 738 915 714 262
Cuts at: 5228 469
Size: 1423
Fragments arranged by size:
1423 915 861 738 714 616 559 262
94
SpeI A'CTAG_T
Cuts at: 67 1641 67
Size: 1574 4608
SphI G_CATG'C
Cuts at: 3235 3735 3235
Size: 500 5682
SspI AAT'ATT
Cuts at: 245 701 2844 245
Size: 456 2143 3583
Fragments arranged by size:
3583 2143 456
StuI AGG'CCT
Cuts at: 2442 2442
Size: 6182
StyI C'CwwG_G
Cuts at: 1303 2437 2990 3316 3670 1303
Size: 1134 553 326 354 3815
Fragments arranged by size:
3815 1134 553 354 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2924 3765 3898 4357 4566 2924
Size: 841 133 459 209 4540
Fragments arranged by size:
4540 841 459 209 133
TatI w'GTAC_w
Cuts at: 789 1263 2407 5697 789
Size: 474 1144 3290 1274
Fragments arranged by size:
3290 1274 1144 474
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5699 5731 5699
Size: 32 6150
Tth111I GACn'n_nGTC
Cuts at: 3979 3979
Size: 6182
XbaI T'CTAG_A
Cuts at: 3213 3213
Size: 6182
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1890 3677 1890
Size: 1787 4395
XmaI C'CCGG_G
Cuts at: 3202 3202
Size: 6182
XmnI GAAnn'nnTTC
Cuts at: 1014 1397 2526 2643 2887 1014
Size: 383 1129 117 244 4309
Fragments arranged by size:
4309 1129 383 244 117
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI ApaI AscI
BbvCI BglI BmgBI BmtI Bpu10I BseRI BsiWI BsmBI
BspEI BsrGI BssHII BstEII BstXI BstZ17I CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PmlI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI