Restriction map for
pF1KA0011
(Circular) (Six-base) MAPSORT of: pF1KA0011.seq Check: 3066 from: 1 to: 5846
pF1KA0011 5846 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1722 1722
Size: 5846
AatII G_ACGT'C
Cuts at: 2043 2043
Size: 5846
AccI GT'mk_AC
Cuts at: 176 1644 2884 176
Size: 1468 1240 3138
Fragments arranged by size:
3138 1468 1240
Acc65I G'GTAC_C
Cuts at: 2862 2862
Size: 5846
AclI AA'CG_TT
Cuts at: 5686 5686
Size: 5846
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 347 589 676 725 890 941 1226 2426
Size: 138 242 87 49 165 51 285 1200
Cuts at: 2426 3671 4103 4456 5118 209
Size: 1245 432 353 662 937
Fragments arranged by size:
1245 1200 937 662 432 353 285 242
165 138 87 51 49
AfeI AGC'GCT
Cuts at: 1264 2071 1264
Size: 807 5039
AflIII A'CryG_T
Cuts at: 3198 4998 5279 3198
Size: 1800 281 3765
Fragments arranged by size:
3765 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1513 1545 1513
Size: 32 5814
AlwNI CAG_nnn'CTG
Cuts at: 699 3151 4589 699
Size: 2452 1438 1956
Fragments arranged by size:
2452 1956 1438
ApaI G_GGCC'C
Cuts at: 754 895 1479 754
Size: 141 584 5121
Fragments arranged by size:
5121 584 141
ApaLI G'TGCA_C
Cuts at: 4684 4684
Size: 5846
ApoI r'AATT_y
Cuts at: 2023 2850 5199 5455 2023
Size: 827 2349 256 2414
Fragments arranged by size:
2414 2349 827 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5846
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5846
AvaI C'yCGr_G
Cuts at: 2866 2866
Size: 5846
AvrII C'CTAG_G
Cuts at: 388 388
Size: 5846
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 670 703 670
Size: 33 5813
BamHI G'GATC_C
Cuts at: 2245 2871 2245
Size: 626 5220
BanI G'GyrC_C
Cuts at: 1213 1313 1561 2224 2862 3276 3524 3559 1213
Size: 100 248 663 638 414 248 35 3500
Fragments arranged by size:
3500 663 638 414 248 248 100 35
BanII G_rGCy'C
Cuts at: 713 754 895 907 1305 1479 2294 2757 2860
Size: 41 141 12 398 174 815 463 103
Cuts at: 2860 3890 713
Size: 1030 2669
Fragments arranged by size:
2669 1030 815 463 398 174 141 103
41 12
BbeI G_GCGC'C
Cuts at: 3280 3528 3280
Size: 248 5598
BbsI GAAGACnn'nnnn_
Cuts at: 851 851
Size: 5846
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1322 1356 1322
Size: 34 5812
BciVI GTATCCnnnnn_n'
Cuts at: 105 1880 3744 4800 5846 105
Size: 1775 1864 1056 1046 105
Fragments arranged by size:
1864 1775 1056 1046 105
BclI T'GATC_A
Cuts at: 300 3366 300
Size: 3066 2780
BglI GCCn_nnn'nGGC
Cuts at: 676 1666 1803 676
Size: 990 137 4719
Fragments arranged by size:
4719 990 137
BglII A'GATC_T
Cuts at: 354 2379 3361 5398 354
Size: 2025 982 2037 802
Fragments arranged by size:
2037 2025 982 802
BlpI GC'TnA_GC
Cuts at: 1670 2958 1670
Size: 1288 4558
Bme1580I G_kGCm'C
Cuts at: 754 895 932 1318 1479 3471 3564 4688 754
Size: 141 37 386 161 1992 93 1124 1912
Fragments arranged by size:
1992 1912 1124 386 161 141 93 37
BmrI ACTGGGnnnn_n'
Cuts at: 1089 1312 1532 2734 3058 3139 3474 5618 1089
Size: 223 220 1202 324 81 335 2144 1317
Fragments arranged by size:
2144 1317 1202 335 324 223 220 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 332 4213 332
Size: 3881 1965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 321 841 1347 1408 2629 302
Size: 19 520 506 61 1221 3519
Fragments arranged by size:
3519 1221 520 506 61 19
Bpu10I CC'TnA_GC
Cuts at: 917 1780 2126 917
Size: 863 346 4637
Fragments arranged by size:
4637 863 346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1156 1510 2097 3022 3901 4615 4913 1156
Size: 354 587 925 879 714 298 2089
Fragments arranged by size:
2089 925 879 714 587 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 384 493 628 1603 5550 384
Size: 109 135 975 3947 680
Fragments arranged by size:
3947 975 680 135 109
BsaAI yAC'GTr
Cuts at: 3199 3199
Size: 5846
BsaBI GATnn'nnATC
Cuts at: 6 3360 5397 6
Size: 3354 2037 455
Fragments arranged by size:
3354 2037 455
BsaHI Gr'CG_yC
Cuts at: 2040 3277 3525 4227 5723 2040
Size: 1237 248 702 1496 2163
Fragments arranged by size:
2163 1496 1237 702 248
BsaWI w'CCGG_w
Cuts at: 1653 2012 3035 3556 4234 4645 4792 5066 1653
Size: 359 1023 521 678 411 147 274 2433
Fragments arranged by size:
2433 1023 678 521 411 359 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 806 836 1153 1183 2632 2662 806
Size: 30 317 30 1449 30 3990
Fragments arranged by size:
3990 1449 317 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 321 1177 1568 2204 321
Size: 856 391 636 3963
Fragments arranged by size:
3963 856 636 391
BseYI C'CCAG_C
Cuts at: 1265 3271 4029 4694 5102 1265
Size: 2006 758 665 408 2009
Fragments arranged by size:
2009 2006 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 260 260
Size: 5846
BsiEI CG_ry'CG
Cuts at: 104 3388 3434 4664 104
Size: 3284 46 1230 1286
Fragments arranged by size:
3284 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 713 834 907 2294 2860 3638 3828 4688 713
Size: 121 73 1387 566 778 190 860 1871
Fragments arranged by size:
1871 1387 860 778 566 190 121 73
BsmI GAATG_Cn'
Cuts at: 2577 2577
Size: 5846
Bsp1286I G_dGCh'C
Cuts at: 713 754 834 895 907 932 1305 1318 1479
Size: 41 80 61 12 25 373 13 161
Cuts at: 1479 2294 2757 2860 3471 3564 3638 3828 3890
Size: 815 463 103 611 93 74 190 62
Cuts at: 3890 4688 713
Size: 798 1871
Fragments arranged by size:
1871 815 798 611 463 373 190 161
103 93 80 74 62 61 41 25
13 12
BspEI T'CCGG_A
Cuts at: 1653 1653
Size: 5846
BspHI T'CATG_A
Cuts at: 4278 4278
Size: 5846
BspMI ACCTGCnnnn'nnnn_
Cuts at: 124 1160 1722 2896 3110 3412 3793 124
Size: 1036 562 1174 214 302 381 2177
Fragments arranged by size:
2177 1174 1036 562 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1144 1296 4138 4192 5676 5841 50
Size: 1094 152 2842 54 1484 165 55
Fragments arranged by size:
2842 1484 1094 165 152 55 54
BsrDI GCAATG_nn'
Cuts at: 2294 3758 2294
Size: 1464 4382
BsrFI r'CCGG_y
Cuts at: 148 3844 4025 148
Size: 3696 181 1969
Fragments arranged by size:
3696 1969 181
BsrGI T'GTAC_A
Cuts at: 1185 1185
Size: 5846
BssSI C'ACGA_G
Cuts at: 944 4117 4825 944
Size: 3173 708 1965
Fragments arranged by size:
3173 1965 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3223 3223
Size: 5846
BstBI TT'CG_AA
Cuts at: 2691 4207 2691
Size: 1516 4330
BstEII G'GTnAC_C
Cuts at: 2182 2182
Size: 5846
BstXI CCAn_nnnn'nTGG
Cuts at: 1265 1265
Size: 5846
BstYI r'GATC_y
Cuts at: 1 354 785 949 2245 2379 2871 3338 3361
Size: 353 431 164 1296 134 492 467 23
Cuts at: 3361 3696 3942 4248 4260 4346 5398 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1296 1052 492 467 449 431 353 335
306 246 164 134 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 623 746 623
Size: 123 5723
BtgI C'CryG_G
Cuts at: 107 155 835 2706 107
Size: 48 680 1871 3247
Fragments arranged by size:
3247 1871 680 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2806 3796 3977 4140 2806
Size: 990 181 163 4512
Fragments arranged by size:
4512 990 181 163
BtsI GCAGTG_nn'
Cuts at: 975 1694 2207 3089 975
Size: 719 513 882 3732
Fragments arranged by size:
3732 882 719 513
DraI TTT'AAA
Cuts at: 2845 2845
Size: 5846
DrdI GACnn_nn'nnGTC
Cuts at: 3552 4896 3552
Size: 1344 4502
EaeI y'GGCC_r
Cuts at: 405 592 3431 3605 3996 4023 5368 405
Size: 187 2839 174 391 27 1345 883
Fragments arranged by size:
2839 1345 883 391 187 174 27
EagI C'GGCC_G
Cuts at: 3431 3431
Size: 5846
EarI CTCTTCn'nnn_
Cuts at: 228 406 701 812 3869 4079 228
Size: 178 295 111 3057 210 1995
Fragments arranged by size:
3057 1995 295 210 178 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4054 4796 4942 5656 4054
Size: 742 146 714 4244
Fragments arranged by size:
4244 742 714 146
EcoICRI GAG'CTC
Cuts at: 711 905 2292 2858 711
Size: 194 1387 566 3699
Fragments arranged by size:
3699 1387 566 194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 209 302 321 347 589 676 725 841 890
Size: 93 19 26 242 87 49 116 49
Cuts at: 890 941 1226 1347 1408 2426 2629 3671 4103
Size: 51 285 121 61 1018 203 1042 432
Cuts at: 4103 4456 5118 209
Size: 353 662 937
Fragments arranged by size:
1042 1018 937 662 432 353 285 242
203 121 116 93 87 61 51 49
49 26 19
EcoNI CCTnn'n_nnAGG
Cuts at: 142 1156 2219 142
Size: 1014 1063 3769
Fragments arranged by size:
3769 1063 1014
EcoO109I rG'GnC_Cy
Cuts at: 392 436 446 617 750 751 891 1411 1434
Size: 44 10 171 133 1 140 520 23
Cuts at: 1434 1442 1475 1521 1659 1805 2031 2439 2985
Size: 8 33 46 138 146 226 408 546
Cuts at: 2985 392
Size: 3253
Fragments arranged by size:
3253 546 520 408 226 171 146 140
138 133 46 44 33 23 10 8
1
EcoRI G'AATT_C
Cuts at: 2850 2850
Size: 5846
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 697 1137 2091 2220 3865 697
Size: 440 954 129 1645 2678
Fragments arranged by size:
2678 1645 954 440 129
FseI GG_CCGG'CC
Cuts at: 152 152
Size: 5846
FspI TGC'GCA
Cuts at: 2557 3627 2557
Size: 1070 4776
HaeII r_GCGC'y
Cuts at: 1266 2073 3280 3528 4758 5529 1266
Size: 807 1207 248 1230 771 1583
Fragments arranged by size:
1583 1230 1207 807 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 892 924 1153 1185 2376 2408 3688 3718 3720
Size: 32 229 32 1191 32 1280 30 2
Cuts at: 3720 3750 892
Size: 30 2988
Fragments arranged by size:
2988 1280 1191 229 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2481 2885 2481
Size: 404 5442
KasI G'GCGC_C
Cuts at: 3276 3524 3276
Size: 248 5598
KpnI G_GTAC'C
Cuts at: 2866 2866
Size: 5846
MluI A'CGCG_T
Cuts at: 5279 5279
Size: 5846
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 527 2273 2799 2836 4605 4789 527
Size: 1746 526 37 1769 184 1584
Fragments arranged by size:
1769 1746 1584 526 184 37
MscI TGG'CCA
Cuts at: 3607 3607
Size: 5846
MslI CAynn'nnrTG
Cuts at: 169 520 2762 3835 3962 169
Size: 351 2242 1073 127 2053
Fragments arranged by size:
2242 2053 1073 351 127
MspA1I CmG'CkG
Cuts at: 1884 2607 2957 3271 3631 4415 4660 1884
Size: 723 350 314 360 784 245 3070
Fragments arranged by size:
3070 784 723 360 350 314 245
NaeI GCC'GGC
Cuts at: 150 4027 150
Size: 3877 1969
NarI GG'CG_CC
Cuts at: 3277 3525 3277
Size: 248 5598
NcoI C'CATG_G
Cuts at: 107 107
Size: 5846
NgoMIV G'CCGG_C
Cuts at: 148 4025 148
Size: 3877 1969
NspI r_CATG'y
Cuts at: 2562 2899 3399 5002 2562
Size: 337 500 1603 3406
Fragments arranged by size:
3406 1603 500 337
PasI CC'CwG_GG
Cuts at: 138 138
Size: 5846
PciI A'CATG_T
Cuts at: 4998 4998
Size: 5846
PfoI T'CCnGG_A
Cuts at: 183 183
Size: 5846
PmeI GTTT'AAAC
Cuts at: 2845 2845
Size: 5846
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4292 4324 4292
Size: 32 5814
PpuMI rG'GwC_Cy
Cuts at: 1411 1442 1521 2031 2439 1411
Size: 31 79 510 408 4818
Fragments arranged by size:
4818 510 408 79 31
PsiI TTA'TAA
Cuts at: 1835 1835
Size: 5846
PspOMI G'GGCC_C
Cuts at: 750 891 1475 750
Size: 141 584 5121
Fragments arranged by size:
5121 584 141
PstI C_TGCA'G
Cuts at: 1733 1883 2274 2606 2893 3578 1733
Size: 150 391 332 287 685 4001
Fragments arranged by size:
4001 685 391 332 287 150
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5846
PvuII CAG'CTG
Cuts at: 2607 3271 3631 2607
Size: 664 360 4822
Fragments arranged by size:
4822 664 360
RsrII CG'GwC_CG
Cuts at: 4041 4041
Size: 5846
SacI G_AGCT'C
Cuts at: 713 907 2294 2860 713
Size: 194 1387 566 3699
Fragments arranged by size:
3699 1387 566 194
SalI G'TCGA_C
Cuts at: 2883 2883
Size: 5846
SanDI GG'GwC_CC
Cuts at: 2439 2439
Size: 5846
SapI GCTCTTCn'nnn_
Cuts at: 701 3869 4079 701
Size: 3168 210 2468
Fragments arranged by size:
3168 2468 210
SbfI CC_TGCA'GG
Cuts at: 2893 2893
Size: 5846
ScaI AGT'ACT
Cuts at: 1631 5363 1631
Size: 3732 2114
SfcI C'TryA_G
Cuts at: 33 923 1729 1751 1879 2270 2348 2602 2889
Size: 890 806 22 128 391 78 254 287
Cuts at: 2889 3574 4542 4733 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 890 806 685 391 287 254
191 128 78 22
SfoI GGC'GCC
Cuts at: 3278 3526 3278
Size: 248 5598
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5846
SmaI CCC'GGG
Cuts at: 2868 2868
Size: 5846
SmlI C'TyrA_G
Cuts at: 1135 1525 2076 3001 3916 4630 4892 1135
Size: 390 551 925 915 714 262 2089
Fragments arranged by size:
2089 925 915 714 551 390 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5846
SphI G_CATG'C
Cuts at: 2562 2899 3399 2562
Size: 337 500 5009
Fragments arranged by size:
5009 500 337
StuI AGG'CCT
Cuts at: 2200 2200
Size: 5846
StyI C'CwwG_G
Cuts at: 107 388 548 755 1049 1437 2187 2453 2980
Size: 281 160 207 294 388 750 266 527
Cuts at: 2980 3334 107
Size: 354 2619
Fragments arranged by size:
2619 750 527 388 354 294 281 266
207 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1403 3429 3562 4021 4230 1403
Size: 2026 133 459 209 3019
Fragments arranged by size:
3019 2026 459 209 133
TatI w'GTAC_w
Cuts at: 1185 1629 2080 5361 1185
Size: 444 451 3281 1670
Fragments arranged by size:
3281 1670 451 444
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 179 211 402 434 806 838 1239 1271 1485
Size: 32 191 32 372 32 401 32 214
Cuts at: 1485 1517 5363 5395 179
Size: 32 3846 32 630
Fragments arranged by size:
3846 630 401 372 214 191 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3643 3643
Size: 5846
XbaI T'CTAG_A
Cuts at: 2877 2877
Size: 5846
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1486 2704 3341 1486
Size: 1218 637 3991
Fragments arranged by size:
3991 1218 637
XmaI C'CCGG_G
Cuts at: 2866 2866
Size: 5846
ZraI GAC'GTC
Cuts at: 2041 2041
Size: 5846
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AflII AgeI AhdI AleI AlfI AscI BbvCI BfrBI
BmgBI BmtI BsiWI BsmBI BssHII BstZ17I ClaI CspCI
DraIII EcoRV FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI