Restriction map for
pF1KA0010
(Circular) (Six-base) MAPSORT of: pF1KA0010.seq Check: 8894 from: 1 to: 6362
pF1KA0010 6362 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3265 3265
Size: 6362
AccI GT'mk_AC
Cuts at: 3400 3400
Size: 6362
Acc65I G'GTAC_C
Cuts at: 1425 1533 3378 1425
Size: 108 1845 4409
Fragments arranged by size:
4409 1845 108
AclI AA'CG_TT
Cuts at: 444 6202 444
Size: 5758 604
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1315 1528 2470 2746 3163 3292 4187 4619 5366
Size: 213 942 276 417 129 895 432 747
Cuts at: 5366 5634 1315
Size: 268 2043
Fragments arranged by size:
2043 942 895 747 432 417 276 268
213 129
AflIII A'CryG_T
Cuts at: 2052 3714 4818 5795 2052
Size: 1662 1104 977 2619
Fragments arranged by size:
2619 1662 1104 977
AgeI A'CCGG_T
Cuts at: 4750 4750
Size: 6362
AleI CACnn'nnGTG
Cuts at: 2773 2773
Size: 6362
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 586 3283 586
Size: 2697 3665
AlwNI CAG_nnn'CTG
Cuts at: 411 3257 3667 5234 411
Size: 2846 410 1567 1539
Fragments arranged by size:
2846 1567 1539 410
ApaI G_GGCC'C
Cuts at: 1104 1104
Size: 6362
ApaLI G'TGCA_C
Cuts at: 2368 3005 5132 2368
Size: 637 2127 3598
Fragments arranged by size:
3598 2127 637
ApoI r'AATT_y
Cuts at: 698 812 1660 1965 2712 2815 2984 3366 5715
Size: 114 848 305 747 103 169 382 2349
Cuts at: 5715 5971 698
Size: 256 1089
Fragments arranged by size:
2349 1089 848 747 382 305 256 169
114 103
AseI AT'TA_AT
Cuts at: 20 2276 2995 20
Size: 2256 719 3387
Fragments arranged by size:
3387 2256 719
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6362
AvaI C'yCGr_G
Cuts at: 794 2953 3280 3382 794
Size: 2159 327 102 3774
Fragments arranged by size:
3774 2159 327 102
BamHI G'GATC_C
Cuts at: 2360 3387 2360
Size: 1027 5335
BanI G'GyrC_C
Cuts at: 1425 1533 3378 3792 4040 4075 1425
Size: 108 1845 414 248 35 3712
Fragments arranged by size:
3712 1845 414 248 108 35
BanII G_rGCy'C
Cuts at: 995 1104 3376 4406 995
Size: 109 2272 1030 2951
Fragments arranged by size:
2951 2272 1030 109
BbeI G_GCGC'C
Cuts at: 3796 4044 3796
Size: 248 6114
BbsI GAAGACnn'nnnn_
Cuts at: 440 1362 2744 3084 440
Size: 922 1382 340 3718
Fragments arranged by size:
3718 1382 922 340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1745 1779 1745
Size: 34 6328
BciVI GTATCCnnnnn_n'
Cuts at: 3206 4260 5021 6362 3206
Size: 1054 761 1341 3206
Fragments arranged by size:
3206 1341 1054 761
BclI T'GATC_A
Cuts at: 1517 2973 3882 1517
Size: 1456 909 3997
Fragments arranged by size:
3997 1456 909
BfrBI ATG'CAT
Cuts at: 1762 1877 1762
Size: 115 6247
BglII A'GATC_T
Cuts at: 2207 3877 5914 2207
Size: 1670 2037 2655
Fragments arranged by size:
2655 2037 1670
BlpI GC'TnA_GC
Cuts at: 3352 3474 3352
Size: 122 6240
Bme1580I G_kGCm'C
Cuts at: 1104 2372 3009 3987 4080 5136 1104
Size: 1268 637 978 93 1056 2330
Fragments arranged by size:
2330 1268 1056 978 637 93
BmgBI CAC'GTC
Cuts at: 2109 2667 2109
Size: 558 5804
BmrI ACTGGGnnnn_n'
Cuts at: 3574 3655 3990 6134 3574
Size: 81 335 2144 3802
Fragments arranged by size:
3802 2144 335 81
BmtI G_CTAG'C
Cuts at: 674 674
Size: 6362
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4729 4729
Size: 6362
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2315 3117 2315
Size: 802 5560
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1227 3253 3538 4417 4909 5207 1227
Size: 2026 285 879 492 298 2382
Fragments arranged by size:
2382 2026 879 492 298 285
BsaI GGTCTCn'nnnn_
Cuts at: 740 6066 740
Size: 5326 1036
BsaAI yAC'GTr
Cuts at: 3715 3715
Size: 6362
BsaBI GATnn'nnATC
Cuts at: 6 3876 5913 6
Size: 3870 2037 455
Fragments arranged by size:
3870 2037 455
BsaHI Gr'CG_yC
Cuts at: 3793 4041 4743 6239 3793
Size: 248 702 1496 3916
Fragments arranged by size:
3916 1496 702 248
BsaWI w'CCGG_w
Cuts at: 864 2852 3551 4072 4750 5024 5171 5582 864
Size: 1988 699 521 678 274 147 411 1644
Fragments arranged by size:
1988 1644 699 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2671 2701 3208 3238 2671
Size: 30 507 30 5795
Fragments arranged by size:
5795 507 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 919 1228 1231 1378 1974 2764 919
Size: 309 3 147 596 790 4517
Fragments arranged by size:
4517 790 596 309 147 3
BseYI C'CCAG_C
Cuts at: 3787 4545 5122 5618 3787
Size: 758 577 496 4531
Fragments arranged by size:
4531 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1429 1429
Size: 6362
BsiEI CG_ry'CG
Cuts at: 104 3904 3950 5158 104
Size: 3800 46 1208 1308
Fragments arranged by size:
3800 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1053 2372 3009 3376 4154 4344 5136 1053
Size: 1319 637 367 778 190 792 2279
Fragments arranged by size:
2279 1319 792 778 637 367 190
BsmI GAATG_Cn'
Cuts at: 623 1008 1294 2582 623
Size: 385 286 1288 4403
Fragments arranged by size:
4403 1288 385 286
Bsp1286I G_dGCh'C
Cuts at: 995 1053 1104 2372 3009 3376 3987 4080 4154
Size: 58 51 1268 637 367 611 93 74
Cuts at: 4154 4344 4406 5136 995
Size: 190 62 730 2221
Fragments arranged by size:
2221 1268 730 637 611 367 190 93
74 62 58 51
BspEI T'CCGG_A
Cuts at: 864 5582 864
Size: 4718 1644
BspHI T'CATG_A
Cuts at: 5538 5538
Size: 6362
BspMI ACCTGCnnnn'nnnn_
Cuts at: 554 2790 3265 3275 3412 3626 3928 4309 554
Size: 2236 475 10 137 214 302 381 2607
Fragments arranged by size:
2607 2236 475 381 302 214 137 10
BsrBI CCG'CTC
Cuts at: 50 3237 4654 4708 6192 6357 50
Size: 3187 1417 54 1484 165 55
Fragments arranged by size:
3187 1484 1417 165 55 54
BsrDI GCAATG_nn'
Cuts at: 368 838 4274 368
Size: 470 3436 2456
Fragments arranged by size:
3436 2456 470
BsrFI r'CCGG_y
Cuts at: 4360 4541 4750 4360
Size: 181 209 5972
Fragments arranged by size:
5972 209 181
BssSI C'ACGA_G
Cuts at: 1956 4633 4991 1956
Size: 2677 358 3327
Fragments arranged by size:
3327 2677 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3739 3739
Size: 6362
BstBI TT'CG_AA
Cuts at: 119 4723 119
Size: 4604 1758
BstXI CCAn_nnnn'nTGG
Cuts at: 1985 1985
Size: 6362
BstYI r'GATC_y
Cuts at: 1 508 2207 2360 2900 3387 3854 3877 4212
Size: 507 1699 153 540 487 467 23 335
Cuts at: 4212 4458 5470 5556 5568 5914 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1699 1012 540 507 487 467 449 346
335 246 153 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2699 2699
Size: 6362
BtgI C'CryG_G
Cuts at: 1379 2385 1379
Size: 1006 5356
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4312 4493 4656 4312
Size: 181 163 6018
Fragments arranged by size:
6018 181 163
BtsI GCAGTG_nn'
Cuts at: 2910 3605 2910
Size: 695 5667
DraI TTT'AAA
Cuts at: 2438 3361 2438
Size: 923 5439
DraIII CAC_nnn'GTG
Cuts at: 2874 2874
Size: 6362
DrdI GACnn_nn'nnGTC
Cuts at: 2836 4068 4926 2836
Size: 1232 858 4272
Fragments arranged by size:
4272 1232 858
EaeI y'GGCC_r
Cuts at: 3947 4121 4512 4539 5884 3947
Size: 174 391 27 1345 4425
Fragments arranged by size:
4425 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3947 3947
Size: 6362
EarI CTCTTCn'nnn_
Cuts at: 1721 2722 4385 4595 1721
Size: 1001 1663 210 3488
Fragments arranged by size:
3488 1663 1001 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2085 2893 2914 4570 4880 5026 6172 2085
Size: 808 21 1656 310 146 1146 2275
Fragments arranged by size:
2275 1656 1146 808 310 146 21
EcoICRI GAG'CTC
Cuts at: 3374 3374
Size: 6362
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1315 1528 2315 2470 2746 3117 3163 3292 4187
Size: 213 787 155 276 371 46 129 895
Cuts at: 4187 4619 5366 5634 1315
Size: 432 747 268 2043
Fragments arranged by size:
2043 895 787 747 432 371 276 268
213 155 129 46
EcoNI CCTnn'n_nnAGG
Cuts at: 1108 2791 1108
Size: 1683 4679
EcoO109I rG'GnC_Cy
Cuts at: 1100 1101 2931 3030 3501 1100
Size: 1 1830 99 471 3961
Fragments arranged by size:
3961 1830 471 99 1
EcoRI G'AATT_C
Cuts at: 1660 3366 1660
Size: 1706 4656
EcoRV GAT'ATC
Cuts at: 1553 1553
Size: 6362
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1456 4381 1456
Size: 2925 3437
FspI TGC'GCA
Cuts at: 971 4143 971
Size: 3172 3190
HaeII r_GCGC'y
Cuts at: 362 961 2849 3796 4044 5066 6045 362
Size: 599 1888 947 248 1022 979 679
Fragments arranged by size:
1888 1022 979 947 679 599 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1225 1257 2192 2224 3022 3054 3158 3190 4204
Size: 32 935 32 798 32 104 32 1014
Cuts at: 4204 4234 4236 4266 1225
Size: 30 2 30 3321
Fragments arranged by size:
3321 1014 935 798 104 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1508 3401 1508
Size: 1893 4469
HindIII A'AGCT_T
Cuts at: 760 760
Size: 6362
KasI G'GCGC_C
Cuts at: 3792 4040 3792
Size: 248 6114
KpnI G_GTAC'C
Cuts at: 1429 1537 3382 1429
Size: 108 1845 4409
Fragments arranged by size:
4409 1845 108
MluI A'CGCG_T
Cuts at: 5795 5795
Size: 6362
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 482 3251 5033 5217 482
Size: 2769 1782 184 1627
Fragments arranged by size:
2769 1782 1627 184
MscI TGG'CCA
Cuts at: 4123 4123
Size: 6362
MslI CAynn'nnrTG
Cuts at: 1378 1402 1882 2608 2773 3259 4351 4478 1378
Size: 24 480 726 165 486 1092 127 3262
Fragments arranged by size:
3262 1092 726 486 480 165 127 24
MspA1I CmG'CkG
Cuts at: 2091 2130 3340 3473 3787 4147 5160 5405 2091
Size: 39 1210 133 314 360 1013 245 3048
Fragments arranged by size:
3048 1210 1013 360 314 245 133 39
NaeI GCC'GGC
Cuts at: 4543 4543
Size: 6362
NarI GG'CG_CC
Cuts at: 3793 4041 3793
Size: 248 6114
NcoI C'CATG_G
Cuts at: 1379 2385 1379
Size: 1006 5356
NgoMIV G'CCGG_C
Cuts at: 4541 4541
Size: 6362
NheI G'CTAG_C
Cuts at: 670 670
Size: 6362
NsiI A_TGCA'T
Cuts at: 1764 1879 1764
Size: 115 6247
NspI r_CATG'y
Cuts at: 527 1766 2056 2607 3415 3915 4822 527
Size: 1239 290 551 808 500 907 2067
Fragments arranged by size:
2067 1239 907 808 551 500 290
PacI TTA_AT'TAA
Cuts at: 473 473
Size: 6362
PciI A'CATG_T
Cuts at: 2052 4818 2052
Size: 2766 3596
PflMI CCAn_nnn'nTGG
Cuts at: 2391 2874 2391
Size: 483 5879
PmeI GTTT'AAAC
Cuts at: 3361 3361
Size: 6362
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1014 1046 5501 5533 1014
Size: 32 4455 32 1843
Fragments arranged by size:
4455 1843 32 32
PpuMI rG'GwC_Cy
Cuts at: 3030 3030
Size: 6362
PspOMI G'GGCC_C
Cuts at: 1100 1100
Size: 6362
PspXI vC'TCGA_Gb
Cuts at: 2953 2953
Size: 6362
PstI C_TGCA'G
Cuts at: 1145 2096 3072 3409 4094 1145
Size: 951 976 337 685 3413
Fragments arranged by size:
3413 976 951 685 337
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6362
PvuII CAG'CTG
Cuts at: 3787 4147 3787
Size: 360 6002
RsrII CG'GwC_CG
Cuts at: 4557 4557
Size: 6362
SacI G_AGCT'C
Cuts at: 3376 3376
Size: 6362
SalI G'TCGA_C
Cuts at: 3399 3399
Size: 6362
SapI GCTCTTCn'nnn_
Cuts at: 1721 2722 4385 4595 1721
Size: 1001 1663 210 3488
Fragments arranged by size:
3488 1663 1001 210
SbfI CC_TGCA'GG
Cuts at: 3409 3409
Size: 6362
ScaI AGT'ACT
Cuts at: 5879 5879
Size: 6362
SexAI A'CCwGG_T
Cuts at: 1337 1337
Size: 6362
SfcI C'TryA_G
Cuts at: 33 282 803 1141 1446 2092 2883 3068 3405
Size: 249 521 338 305 646 791 185 337
Cuts at: 3405 4090 5083 5274 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 791 685 646 521 338 337
305 249 191 185
SfoI GGC'GCC
Cuts at: 3794 4042 3794
Size: 248 6114
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6362
SmaI CCC'GGG
Cuts at: 3384 3384
Size: 6362
SmlI C'TyrA_G
Cuts at: 794 1242 2953 3232 3517 4432 4924 5186 794
Size: 448 1711 279 285 915 492 262 1970
Fragments arranged by size:
1970 1711 915 492 448 285 279 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6362
SphI G_CATG'C
Cuts at: 1766 3415 3915 1766
Size: 1649 500 4213
Fragments arranged by size:
4213 1649 500
SspI AAT'ATT
Cuts at: 304 781 304
Size: 477 5885
StyI C'CwwG_G
Cuts at: 143 153 1379 2385 3496 3850 143
Size: 10 1226 1006 1111 354 2655
Fragments arranged by size:
2655 1226 1111 1006 354 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3945 4078 4537 4746 3945
Size: 133 459 209 5561
Fragments arranged by size:
5561 459 209 133
TatI w'GTAC_w
Cuts at: 5877 5877
Size: 6362
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2671 2703 5879 5911 2671
Size: 32 3176 32 3122
Fragments arranged by size:
3176 3122 32 32
Tth111I GACn'n_nGTC
Cuts at: 4159 4159
Size: 6362
XbaI T'CTAG_A
Cuts at: 3393 3393
Size: 6362
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2106 2664 3857 2106
Size: 558 1193 4611
Fragments arranged by size:
4611 1193 558
XhoI C'TCGA_G
Cuts at: 794 2953 794
Size: 2159 4203
XmaI C'CCGG_G
Cuts at: 3382 3382
Size: 6362
XmnI GAAnn'nnTTC
Cuts at: 2501 2501
Size: 6362
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PacI
PciI PflMI PmeI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BglI Bpu10I BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI CspCI FseI FspAI HpaI MfeI NdeI
NotI NruI PasI PfoI PmlI PshAI PsiI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
ZraI