Restriction map for
pF1KA0003
(Circular) (Six-base) MAPSORT of: pF1KA0003.seq Check: 6768 from: 1 to: 4607
pF1KA0003 4607 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 1645 1645
Size: 4607
Acc65I G'GTAC_C
Cuts at: 1623 1623
Size: 4607
AclI AA'CG_TT
Cuts at: 4447 4447
Size: 4607
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 2432 2864 3217 3879 832
Size: 1600 432 353 662 1560
Fragments arranged by size:
1600 1560 662 432 353
AflIII A'CryG_T
Cuts at: 324 369 1959 3759 4040 324
Size: 45 1590 1800 281 891
Fragments arranged by size:
1800 1590 891 281 45
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 332 332
Size: 4607
AlwNI CAG_nnn'CTG
Cuts at: 396 597 804 861 1912 3350 396
Size: 201 207 57 1051 1438 1653
Fragments arranged by size:
1653 1438 1051 207 201 57
ApaI G_GGCC'C
Cuts at: 1553 1553
Size: 4607
ApaLI G'TGCA_C
Cuts at: 3445 3445
Size: 4607
ApoI r'AATT_y
Cuts at: 603 1611 3960 4216 603
Size: 1008 2349 256 994
Fragments arranged by size:
2349 1008 994 256
AseI AT'TA_AT
Cuts at: 20 1019 20
Size: 999 3608
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4607
AvaI C'yCGr_G
Cuts at: 1627 1627
Size: 4607
BamHI G'GATC_C
Cuts at: 1632 1632
Size: 4607
BanI G'GyrC_C
Cuts at: 1623 2037 2285 2320 1623
Size: 414 248 35 3910
Fragments arranged by size:
3910 414 248 35
BanII G_rGCy'C
Cuts at: 1553 1621 2651 1553
Size: 68 1030 3509
Fragments arranged by size:
3509 1030 68
BbeI G_GCGC'C
Cuts at: 2041 2289 2041
Size: 248 4359
BciVI GTATCCnnnnn_n'
Cuts at: 2505 3561 4607 2505
Size: 1056 1046 2505
Fragments arranged by size:
2505 1056 1046
BclI T'GATC_A
Cuts at: 2127 2127
Size: 4607
BglII A'GATC_T
Cuts at: 2122 4159 2122
Size: 2037 2570
BlpI GC'TnA_GC
Cuts at: 1719 1719
Size: 4607
Bme1580I G_kGCm'C
Cuts at: 1428 1553 2232 2325 3449 1428
Size: 125 679 93 1124 2586
Fragments arranged by size:
2586 1124 679 125 93
BmrI ACTGGGnnnn_n'
Cuts at: 1254 1343 1547 1819 1900 2235 4379 1254
Size: 89 204 272 81 335 2144 1482
Fragments arranged by size:
2144 1482 335 272 204 89 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 335 2974 335
Size: 2639 1968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 619 883 511
Size: 108 264 4235
Fragments arranged by size:
4235 264 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 604 1783 2662 3376 3674 427
Size: 177 1179 879 714 298 1360
Fragments arranged by size:
1360 1179 879 714 298 177
BsaI GGTCTCn'nnnn_
Cuts at: 547 4311 547
Size: 3764 843
BsaAI yAC'GTr
Cuts at: 327 1960 327
Size: 1633 2974
BsaBI GATnn'nnATC
Cuts at: 6 974 2121 4158 6
Size: 968 1147 2037 455
Fragments arranged by size:
2037 1147 968 455
BsaHI Gr'CG_yC
Cuts at: 2038 2286 2988 4484 2038
Size: 248 702 1496 2161
Fragments arranged by size:
2161 1496 702 248
BsaWI w'CCGG_w
Cuts at: 209 333 1162 1796 2317 2995 3406 3553 3827
Size: 124 829 634 521 678 411 147 274
Cuts at: 3827 209
Size: 989
Fragments arranged by size:
989 829 678 634 521 411 274 147
124
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 499 499
Size: 4607
BseYI C'CCAG_C
Cuts at: 1176 2032 2790 3455 3863 1176
Size: 856 758 665 408 1920
Fragments arranged by size:
1920 856 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 986 183
Size: 803 3804
BsiEI CG_ry'CG
Cuts at: 104 2149 2195 3425 104
Size: 2045 46 1230 1286
Fragments arranged by size:
2045 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1621 2399 2589 3449 1621
Size: 778 190 860 2779
Fragments arranged by size:
2779 860 778 190
BsmI GAATG_Cn'
Cuts at: 465 465
Size: 4607
Bsp1286I G_dGCh'C
Cuts at: 1428 1553 1621 2232 2325 2399 2589 2651 3449
Size: 125 68 611 93 74 190 62 798
Cuts at: 3449 1428
Size: 2586
Fragments arranged by size:
2586 798 611 190 125 93 74 68
62
BspHI T'CATG_A
Cuts at: 3039 3039
Size: 4607
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1657 1871 2173 2554 1657
Size: 214 302 381 3710
Fragments arranged by size:
3710 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2899 2953 4437 4602 50
Size: 2849 54 1484 165 55
Fragments arranged by size:
2849 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 232 2519 232
Size: 2287 2320
BsrFI r'CCGG_y
Cuts at: 2605 2786 2605
Size: 181 4426
BssSI C'ACGA_G
Cuts at: 2878 3586 2878
Size: 708 3899
BstAPI GCAn_nnn'nTGC
Cuts at: 1221 1984 1221
Size: 763 3844
BstBI TT'CG_AA
Cuts at: 2968 2968
Size: 4607
BstYI r'GATC_y
Cuts at: 1 665 1632 2099 2122 2457 2703 3009 3021
Size: 664 967 467 23 335 246 306 12
Cuts at: 3021 3107 4159 1
Size: 86 1052 449
Fragments arranged by size:
1052 967 664 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 1509 1509
Size: 4607
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2557 2738 2901 2557
Size: 181 163 4263
Fragments arranged by size:
4263 181 163
BtsI GCAGTG_nn'
Cuts at: 1850 1850
Size: 4607
DraI TTT'AAA
Cuts at: 1322 1606 1322
Size: 284 4323
DrdI GACnn_nn'nnGTC
Cuts at: 2313 3657 2313
Size: 1344 3263
EaeI y'GGCC_r
Cuts at: 2192 2366 2757 2784 4129 2192
Size: 174 391 27 1345 2670
Fragments arranged by size:
2670 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2192 2192
Size: 4607
EarI CTCTTCn'nnn_
Cuts at: 348 722 743 938 2630 2840 348
Size: 374 21 195 1692 210 2115
Fragments arranged by size:
2115 1692 374 210 195 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2815 3557 3703 4417 2815
Size: 742 146 714 3005
Fragments arranged by size:
3005 742 714 146
EcoICRI GAG'CTC
Cuts at: 1619 1619
Size: 4607
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 619 832 883 2432 2864 3217 3879 511
Size: 108 213 51 1549 432 353 662 1239
Fragments arranged by size:
1549 1239 662 432 353 213 108 51
EcoO109I rG'GnC_Cy
Cuts at: 1549 1746 1549
Size: 197 4410
EcoRI G'AATT_C
Cuts at: 603 1611 603
Size: 1008 3599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 778 2626 778
Size: 1848 2759
FspI TGC'GCA
Cuts at: 2388 2388
Size: 4607
HaeII r_GCGC'y
Cuts at: 177 2041 2289 3519 4290 177
Size: 1864 248 1230 771 494
Fragments arranged by size:
1864 1230 771 494 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2449 2479 2481 2511 2449
Size: 30 2 30 4545
Fragments arranged by size:
4545 30 30 2
HincII GTy'rAC
Cuts at: 1436 1646 1436
Size: 210 4397
HpaI GTT'AAC
Cuts at: 1436 1436
Size: 4607
KasI G'GCGC_C
Cuts at: 2037 2285 2037
Size: 248 4359
KpnI G_GTAC'C
Cuts at: 1627 1627
Size: 4607
MluI A'CGCG_T
Cuts at: 4040 4040
Size: 4607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 416 713 1057 3366 3550 416
Size: 297 344 2309 184 1473
Fragments arranged by size:
2309 1473 344 297 184
MscI TGG'CCA
Cuts at: 2368 2368
Size: 4607
MslI CAynn'nnrTG
Cuts at: 1576 2596 2723 1576
Size: 1020 127 3460
Fragments arranged by size:
3460 1020 127
MspA1I CmG'CkG
Cuts at: 594 1718 2032 2392 3176 3421 594
Size: 1124 314 360 784 245 1780
Fragments arranged by size:
1780 1124 784 360 314 245
NaeI GCC'GGC
Cuts at: 2788 2788
Size: 4607
NarI GG'CG_CC
Cuts at: 2038 2286 2038
Size: 248 4359
NcoI C'CATG_G
Cuts at: 1509 1509
Size: 4607
NgoMIV G'CCGG_C
Cuts at: 2786 2786
Size: 4607
NspI r_CATG'y
Cuts at: 373 1224 1660 2160 3763 373
Size: 851 436 500 1603 1217
Fragments arranged by size:
1603 1217 851 500 436
PciI A'CATG_T
Cuts at: 369 3759 369
Size: 3390 1217
PflMI CCAn_nnn'nTGG
Cuts at: 804 804
Size: 4607
PfoI T'CCnGG_A
Cuts at: 797 797
Size: 4607
PmeI GTTT'AAAC
Cuts at: 1606 1606
Size: 4607
PmlI CAC'GTG
Cuts at: 327 327
Size: 4607
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3053 3085 3053
Size: 32 4575
PspOMI G'GGCC_C
Cuts at: 1549 1549
Size: 4607
PstI C_TGCA'G
Cuts at: 311 525 1654 2339 311
Size: 214 1129 685 2579
Fragments arranged by size:
2579 1129 685 214
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4607
PvuII CAG'CTG
Cuts at: 594 2032 2392 594
Size: 1438 360 2809
Fragments arranged by size:
2809 1438 360
RsrII CG'GwC_CG
Cuts at: 2802 2802
Size: 4607
SacI G_AGCT'C
Cuts at: 1621 1621
Size: 4607
SalI G'TCGA_C
Cuts at: 1644 1644
Size: 4607
SapI GCTCTTCn'nnn_
Cuts at: 2630 2840 2630
Size: 210 4397
SbfI CC_TGCA'GG
Cuts at: 1654 1654
Size: 4607
ScaI AGT'ACT
Cuts at: 4124 4124
Size: 4607
SfcI C'TryA_G
Cuts at: 33 307 521 1308 1650 2335 3303 3494 33
Size: 274 214 787 342 685 968 191 1146
Fragments arranged by size:
1146 968 787 685 342 274 214 191
SfoI GGC'GCC
Cuts at: 2039 2287 2039
Size: 248 4359
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4607
SmaI CCC'GGG
Cuts at: 1629 1629
Size: 4607
SmlI C'TyrA_G
Cuts at: 406 583 1762 2677 3391 3653 406
Size: 177 1179 915 714 262 1360
Fragments arranged by size:
1360 1179 915 714 262 177
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4607
SphI G_CATG'C
Cuts at: 1660 2160 1660
Size: 500 4107
SspI AAT'ATT
Cuts at: 1171 1171
Size: 4607
StyI C'CwwG_G
Cuts at: 1509 1741 2095 1509
Size: 232 354 4021
Fragments arranged by size:
4021 354 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2190 2323 2782 2991 2190
Size: 133 459 209 3806
Fragments arranged by size:
3806 459 209 133
TatI w'GTAC_w
Cuts at: 290 1217 4122 290
Size: 927 2905 775
Fragments arranged by size:
2905 927 775
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4124 4156 4124
Size: 32 4575
Tth111I GACn'n_nGTC
Cuts at: 877 2404 877
Size: 1527 3080
XbaI T'CTAG_A
Cuts at: 1110 1638 1110
Size: 528 4079
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2102 2102
Size: 4607
XmaI C'CCGG_G
Cuts at: 1627 1627
Size: 4607
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlfI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmBI BspEI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII MfeI NdeI NheI
NotI NruI NsiI PacI PasI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI