Restriction map for
pF1KE4446
(Circular) (Six-base) MAPSORT of: pF1K.seq Check: 1693 from: 1 to: 3450
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AatII G_ACGT'C
Cuts at: 245 245
Size: 3450
AccI GT'mk_AC
Cuts at: 488 488
Size: 3450
Acc65I G'GTAC_C
Cuts at: 466 466
Size: 3450
AclI AA'CG_TT
Cuts at: 3290 3290
Size: 3450
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 339 1275 1707 2454 2722 136
Size: 203 936 432 747 268 864
Fragments arranged by size:
936 864 747 432 268 203
AflIII A'CryG_T
Cuts at: 125 802 1906 2883 125
Size: 677 1104 977 692
Fragments arranged by size:
1104 977 692 677
AgeI A'CCGG_T
Cuts at: 1838 1838
Size: 3450
AlwNI CAG_nnn'CTG
Cuts at: 755 2322 755
Size: 1567 1883
ApaLI G'TGCA_C
Cuts at: 2220 2220
Size: 3450
ApoI r'AATT_y
Cuts at: 360 454 2803 3059 360
Size: 94 2349 256 751
Fragments arranged by size:
2349 751 256 94
AseI AT'TA_AT
Cuts at: 20 20
Size: 3450
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3450
AvaI C'yCGr_G
Cuts at: 470 470
Size: 3450
BamHI G'GATC_C
Cuts at: 475 475
Size: 3450
BanI G'GyrC_C
Cuts at: 466 880 1128 1163 466
Size: 414 248 35 2753
Fragments arranged by size:
2753 414 248 35
BanII G_rGCy'C
Cuts at: 464 1494 464
Size: 1030 2420
BbeI G_GCGC'C
Cuts at: 884 1132 884
Size: 248 3202
BciVI GTATCCnnnnn_n'
Cuts at: 1348 2109 3450 1348
Size: 761 1341 1348
Fragments arranged by size:
1348 1341 761
BclI T'GATC_A
Cuts at: 970 970
Size: 3450
BglII A'GATC_T
Cuts at: 965 3002 965
Size: 2037 1413
BlpI GC'TnA_GC
Cuts at: 562 562
Size: 3450
Bme1580I G_kGCm'C
Cuts at: 1075 1168 2224 1075
Size: 93 1056 2301
Fragments arranged by size:
2301 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 662 743 1078 3222 662
Size: 81 335 2144 890
Fragments arranged by size:
2144 890 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1817 1817
Size: 3450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 283
Size: 3450
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 366 626 1505 1997 2295 366
Size: 260 879 492 298 1521
Fragments arranged by size:
1521 879 492 298 260
BsaI GGTCTCn'nnnn_
Cuts at: 3154 3154
Size: 3450
BsaAI yAC'GTr
Cuts at: 803 803
Size: 3450
BsaBI GATnn'nnATC
Cuts at: 6 964 3001 6
Size: 958 2037 455
Fragments arranged by size:
2037 958 455
BsaHI Gr'CG_yC
Cuts at: 242 881 1129 1831 3327 242
Size: 639 248 702 1496 365
Fragments arranged by size:
1496 702 639 365 248
BsaWI w'CCGG_w
Cuts at: 368 639 1160 1838 2112 2259 2670 368
Size: 271 521 678 274 147 411 1148
Fragments arranged by size:
1148 678 521 411 274 271 147
BseYI C'CCAG_C
Cuts at: 212 875 1633 2210 2706 212
Size: 663 758 577 496 956
Fragments arranged by size:
956 758 663 577 496
BsiEI CG_ry'CG
Cuts at: 104 992 1038 2246 104
Size: 888 46 1208 1308
Fragments arranged by size:
1308 1208 888 46
BsiHKAI G_wGCw'C
Cuts at: 464 1242 1432 2224 464
Size: 778 190 792 1690
Fragments arranged by size:
1690 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 464 1075 1168 1242 1432 1494 2224 464
Size: 611 93 74 190 62 730 1690
Fragments arranged by size:
1690 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2670 2670
Size: 3450
BspHI T'CATG_A
Cuts at: 2626 2626
Size: 3450
BspMI ACCTGCnnnn'nnnn_
Cuts at: 500 714 1016 1397 500
Size: 214 302 381 2553
Fragments arranged by size:
2553 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1742 1796 3280 3445 50
Size: 1692 54 1484 165 55
Fragments arranged by size:
1692 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1362 1362
Size: 3450
BsrFI r'CCGG_y
Cuts at: 1448 1629 1838 1448
Size: 181 209 3060
Fragments arranged by size:
3060 209 181
BssSI C'ACGA_G
Cuts at: 1721 2079 1721
Size: 358 3092
BstAPI GCAn_nnn'nTGC
Cuts at: 827 827
Size: 3450
BstBI TT'CG_AA
Cuts at: 1811 1811
Size: 3450
BstYI r'GATC_y
Cuts at: 1 475 942 965 1300 1546 2558 2644 2656
Size: 474 467 23 335 246 1012 86 12
Cuts at: 2656 3002 1
Size: 346 449
Fragments arranged by size:
1012 474 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3450
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1400 1581 1744 1400
Size: 181 163 3106
Fragments arranged by size:
3106 181 163
BtsI GCAGTG_nn'
Cuts at: 693 693
Size: 3450
DraI TTT'AAA
Cuts at: 449 449
Size: 3450
DrdI GACnn_nn'nnGTC
Cuts at: 1156 2014 1156
Size: 858 2592
EaeI y'GGCC_r
Cuts at: 1035 1209 1600 1627 2972 1035
Size: 174 391 27 1345 1513
Fragments arranged by size:
1513 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1035 1035
Size: 3450
EarI CTCTTCn'nnn_
Cuts at: 1473 1683 1473
Size: 210 3240
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 281 1658 1968 2114 3260 281
Size: 1377 310 146 1146 471
Fragments arranged by size:
1377 1146 471 310 146
EcoICRI GAG'CTC
Cuts at: 462 462
Size: 3450
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 283 339 1275 1707 2454 2722 136
Size: 147 56 936 432 747 268 864
Fragments arranged by size:
936 864 747 432 268 147 56
EcoO109I rG'GnC_Cy
Cuts at: 589 589
Size: 3450
EcoRI G'AATT_C
Cuts at: 454 454
Size: 3450
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 250 1469 250
Size: 1219 2231
FspI TGC'GCA
Cuts at: 1231 1231
Size: 3450
HaeII r_GCGC'y
Cuts at: 884 1132 2154 3133 884
Size: 248 1022 979 1201
Fragments arranged by size:
1201 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 262 294 1292 1322 1324 1354 262
Size: 32 998 30 2 30 2358
Fragments arranged by size:
2358 998 32 30 30 2
HincII GTy'rAC
Cuts at: 489 489
Size: 3450
KasI G'GCGC_C
Cuts at: 880 1128 880
Size: 248 3202
KpnI G_GTAC'C
Cuts at: 470 470
Size: 3450
MluI A'CGCG_T
Cuts at: 2883 2883
Size: 3450
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2121 2305 2121
Size: 184 3266
MscI TGG'CCA
Cuts at: 1211 1211
Size: 3450
MslI CAynn'nnrTG
Cuts at: 1439 1566 1439
Size: 127 3323
MspA1I CmG'CkG
Cuts at: 561 875 1235 2248 2493 561
Size: 314 360 1013 245 1518
Fragments arranged by size:
1518 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1631 1631
Size: 3450
NarI GG'CG_CC
Cuts at: 881 1129 881
Size: 248 3202
NcoI C'CATG_G
Cuts at: 107 107
Size: 3450
NgoMIV G'CCGG_C
Cuts at: 1629 1629
Size: 3450
NspI r_CATG'y
Cuts at: 503 1003 1910 503
Size: 500 907 2043
Fragments arranged by size:
2043 907 500
PciI A'CATG_T
Cuts at: 1906 1906
Size: 3450
PmeI GTTT'AAAC
Cuts at: 449 449
Size: 3450
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2589 2621 2589
Size: 32 3418
PstI C_TGCA'G
Cuts at: 497 1182 497
Size: 685 2765
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3450
PvuII CAG'CTG
Cuts at: 875 1235 875
Size: 360 3090
RsrII CG'GwC_CG
Cuts at: 1645 1645
Size: 3450
SacI G_AGCT'C
Cuts at: 464 464
Size: 3450
SalI G'TCGA_C
Cuts at: 487 487
Size: 3450
SapI GCTCTTCn'nnn_
Cuts at: 1473 1683 1473
Size: 210 3240
SbfI CC_TGCA'GG
Cuts at: 497 497
Size: 3450
ScaI AGT'ACT
Cuts at: 2967 2967
Size: 3450
SfcI C'TryA_G
Cuts at: 33 493 1178 2171 2362 33
Size: 460 685 993 191 1121
Fragments arranged by size:
1121 993 685 460 191
SfoI GGC'GCC
Cuts at: 882 1130 882
Size: 248 3202
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3450
SmaI CCC'GGG
Cuts at: 472 472
Size: 3450
SmlI C'TyrA_G
Cuts at: 381 605 1520 2012 2274 381
Size: 224 915 492 262 1557
Fragments arranged by size:
1557 915 492 262 224
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3450
SphI G_CATG'C
Cuts at: 503 1003 503
Size: 500 2950
StyI C'CwwG_G
Cuts at: 107 584 938 107
Size: 477 354 2619
Fragments arranged by size:
2619 477 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 204 1033 1166 1625 1834 204
Size: 829 133 459 209 1820
Fragments arranged by size:
1820 829 459 209 133
TatI w'GTAC_w
Cuts at: 2965 2965
Size: 3450
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2967 2999 2967
Size: 32 3418
Tth111I GACn'n_nGTC
Cuts at: 1247 1247
Size: 3450
XbaI T'CTAG_A
Cuts at: 481 481
Size: 3450
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 945 945
Size: 3450
XmaI C'CCGG_G
Cuts at: 470 470
Size: 3450
ZraI GAC'GTC
Cuts at: 243 243
Size: 3450
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI