GENSCAN 1.0	Date run:  7-Nov-116	Time: 21:21:55

Sequence gi568815597r:57956136_58156480 : 200345 bp : 41.65% C+G : Isochore 1 ( 0 - 43 C+G%)

Parameter matrix: HumanIso.smat

Predicted genes/exons:

Gn.Ex Type S .Begin ...End .Len Fr Ph I/Ac Do/T CodRg P.... Tscr..
----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------

 1.01 Init +   1002   1048   47  2  2   45  110    70 0.829   5.11
 1.02 Term +   3818   4040  223  2  1  126   49    88 0.775   4.11
 1.03 PlyA +   4359   4364    6                              -0.45

 2.00 Prom +   4415   4454   40                              -1.85
 2.01 Init +  10999  11055   57  0  0   87   48    44 0.204   1.66
 2.02 Term +  21914  22729  816  1  0   86   50   269 0.597  15.05
 2.03 PlyA +  23451  23456    6                               1.05

 3.00 Prom +  27818  27857   40                              -4.15
 3.01 Init +  28226  28228    3  1  0   48  115     0 0.280  -1.25
 3.02 Intr +  31726  31816   91  0  1  107   56    69 0.072   4.25
 3.03 Intr +  62633  62691   59  0  2  115   86    36 0.027   3.78
 3.04 Term +  82338  82481  144  2  0   83   43   117 0.261   3.63
 3.05 PlyA +  83237  83242    6                               1.05

 4.08 PlyA -  83694  83689    6                               1.05
 4.07 Term -  92674  92174  501  1  0    7   49   529 0.329  34.39
 4.06 Intr -  93045  92923  123  0  0  -28   98   157 0.563   4.96
 4.05 Intr -  96645  96495  151  2  1  -49   86    95 0.082  -5.16
 4.04 Intr - 100345 100002  344  1  2   50   75   382 0.011  26.40
 4.03 Intr - 104167 104090   78  1  0   78   72    49 0.007   1.13
 4.02 Intr - 104633 104452  182  0  2   89   98    81 0.028   7.87
 4.01 Init - 106460 106337  124  2  1   61   37   126 0.025   5.18
 4.00 Prom - 110613 110574   40                              -5.95

 5.00 Prom + 112206 112245   40                              -0.25
 5.01 Init + 115087 115228  142  0  1   69   66    79 0.731   4.15
 5.02 Intr + 121854 121928   75  1  0  120  121    15 0.923   6.57
 5.03 Intr + 123876 123971   96  1  0  118   68    59 0.478   5.96
 5.04 Intr + 137024 137162  139  0  1   19   86   146 0.196   6.10
 5.05 Term + 138304 138421  118  1  1  104   38    60 0.332  -0.37
 5.06 PlyA + 139064 139069    6                               1.05

 6.00 Prom + 140224 140263   40                              -9.75
 6.01 Init + 141042 141256  215  2  2   93  121   103 0.958  12.36
 6.02 Intr + 142055 142137   83  2  2   94   44    26 0.938  -2.84
 6.03 Term + 142781 142944  164  0  2   43   47   196 0.900   8.12
 6.04 PlyA + 144680 144685    6                               1.05

 7.00 Prom + 147109 147148   40                              -2.55
 7.01 Init + 151813 152019  207  0  0   64   93   138 0.878  10.77
 7.02 Term + 153205 153369  165  0  0   20   39   142 0.419  -0.57
 7.03 PlyA + 155372 155377    6                               1.05

 8.03 PlyA - 156126 156121    6                               1.05
 8.02 Term - 157637 157029  609  2  0   95   38   195 0.251   8.71
 8.01 Init - 173569 171908 1662  1  0   66   86   555 0.195  44.98
 8.00 Prom - 174030 173991   40                              -6.15

 9.02 PlyA - 174199 174194    6                               1.05
 9.01 Sngl - 175443 174667  777  0  0   88   46   631 0.979  54.66
 9.00 Prom - 176792 176753   40                              -5.45

10.05 PlyA - 177465 177460    6                               1.05
10.04 Term - 177708 177598  111  0  0   -1   43    91 0.135  -6.72
10.03 Intr - 178131 178032  100  2  1   51   37   157 0.030   6.09
10.02 Intr - 186900 186767  134  0  2   26   81   102 0.021   1.82
10.01 Intr - 197191 197139   53  2  2  118   89    51 0.844   5.91

Suboptimal exons with probability > 0.800

Exnum Type S .Begin ...End .Len Fr Ph B/Ac Do/T CodRg P.... Tscr..
----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------



Predicted peptide sequence(s):

Predicted coding sequence(s):


>gi568815597r:57956136_58156480|GENSCAN_predicted_peptide_1|89_aa
MCEEIVQGHDWMSRTRQPLHKNIHSPTVHQLFFLLLAYLMQHPQMPERVSAAASTMPGLI
AIRSCYGEECGRGGRDGVVWHGKPRPCGN

>gi568815597r:57956136_58156480|GENSCAN_predicted_CDS_1|270_bp
atgtgtgaagagattgtacagggacatgactggatgtcgaggaccaggcaacccttgcat
aaaaatatccactcaccaactgttcaccaactgttcttcctcttgctggcataccttatg
cagcatcctcagatgccagagcgcgtttctgctgcagcctccacgatgcctgggctgata
gcaatcaggtcctgctatggtgaggagtgtgggagaggagggagagatggtgttgtctgg
cacgggaagcccaggccgtgtggcaattag

>gi568815597r:57956136_58156480|GENSCAN_predicted_peptide_2|290_aa
MQIVGHLSMGATATVGLSKNCKKITLKFIWNRKRACIAKKILGRKNKAGGIMLPDFKLHY
KATVTKTAWYWYQNRHIDQWNRTEASEITPHIYNNLIFDKPETNKQWGKDALFNKWCWEN
WLVMCRKLKQDPFLTPYIKINSRWIKDSNVRPRTIKILEENLGNTIEDTGTGKDFMSRTP
KAMATKAKIDKWDLIKLNGFCKAKETIIRVNRQPTKWEKNFAIYPSDKGLISRIHKELKQ
IYKKKTNNPIKKWAKDMNRHFSKEDIYAASRHMKNAHHHWSLWKCKSKPQ

>gi568815597r:57956136_58156480|GENSCAN_predicted_CDS_2|873_bp
atgcagattgtaggtcacctttccatgggagccacagctacagtggggctgtccaagaat
tgcaaaaaaattactttaaagttcatatggaaccgaaaaagagcctgcatagccaagaaa
atcctgggcaggaagaacaaagctggaggcattatgctacctgacttcaaactacactac
aaggctacagtaaccaaaacagcatggtactggtaccaaaacagacatatagaccagtgg
aacagaacagaggcctcagaaataacaccacacatctacaacaatctgatctttgacaaa
cctgaaacaaataagcaatggggaaaagatgccctatttaataaatggtgctgggaaaac
tggctagtcatgtgcagaaaactgaaacaggaccccttccttacaccttatataaaaatc
aactcaagatggatcaaagactcaaacgtaagacccagaaccataaaaatcctagaagaa
aacctgggcaataccattgaggacacaggcaccggaaaagacttcatgtctagaacacca
aaagcaatggcaacaaaagccaaaattgacaaatgggatctaattaaactaaacggcttc
tgcaaagcaaaagaaactatcatcagagtcaacaggcaacctacaaaatgggagaaaaat
tttgcaatctatccatctgacaaagggctaatatccagaatccacaaagaacttaaacaa
atttacaagaaaaaaacaaacaaccccatcaaaaaatgggcaaaggatatgaacagacac
ttctcaaaagaagacatttatgcagccagcagacatatgaaaaatgctcatcatcactgg
tcattatggaaatgcaaatcaaaaccacaatga

>gi568815597r:57956136_58156480|GENSCAN_predicted_peptide_3|98_aa
MENTGRLLMEKKKKQQRQRVYLGCDRMPGASGPSKSSLKNSKKYIVIKLLWEREKETFVN
QRLPTEHSAMTVVLHQGFLSPGFNKLLFLPNPKHATGT

>gi568815597r:57956136_58156480|GENSCAN_predicted_CDS_3|297_bp
atggaaaacactggtcggttactaatggagaagaagaaaaagcagcaaagacaacgagta
tacttgggatgcgatcgcatgcctggagcatctggcccttccaagtcttccctgaaaaac
tcaaagaagtacattgttataaaactcctatgggaaagagaaaaggaaacatttgtgaat
cagcggttaccaactgagcactcagccatgaccgtggttcttcaccagggcttcctctca
cctgggtttaataagctcctttttttaccaaatccaaagcatgcaactggcacttaa

>gi568815597r:57956136_58156480|GENSCAN_predicted_peptide_4|500_aa
MPCSVTVDSVEQQSSKMKNGCFKEEARIVVWKVLEAGIAEAAHGCWPRKPRIGSLPWDLC
TLTYNTRGMLLCSQARTLWALGQETPKTPQRVQKGQQQRNCQRASREACWKELAFGFLLK
LQIQPLHSMTKKRRNNGRAKKGRGHVQPIRCTNCARCVPKDKAIKTFVIRNIVKAAAVRD
ISEASVFDAYVLPKLYVKLHYCVSCAIHSKVVRNRSREARKDRTPPSRFRPAGAAPRPPP
KPMNPTYKGCEDLFKENYKPLLNEIKEDRNKWKNIPCLWIGRINIMKMAILPKGTLTDCV
VLRDPNTKCSGGFGFVTYATVEEVDAATNARPHKTVIQKYYTVNGHNCEVRKALSKQEMA
SVSSSQRGEVVLETLVVVVEVVSVGMTTLVVEETLVVMVALVAAVMSVDMVAVGMVIMGL
AIMVVMEEVALVTLEEAEAMEVVDRVMETRAVAMAGVAAMTAITIEVEVALAVAVEAVLE
VVEATVIWAIKTISFQILDP

>gi568815597r:57956136_58156480|GENSCAN_predicted_CDS_4|1503_bp
atgccttgctctgtgactgtggactctgtggagcagcagagcagcaagatgaaaaatgga
tgtttcaaagaggaagcaaggatagttgtatggaaggtcttggaagctggaatagcagag
gcagcccatggctgctggcccagaaagccgagaattggcagtttaccctgggacttgtgc
acactcacttacaacactcgaggcatgcttctctgttctcaggccaggactttgtgggcc
cttgggcaggagaccccgaaaaccccacagagggtgcagaaagggcagcaacagaggaac
tgtcagagagccagcagagaagcctgttggaaagagttggcttttggttttcttcttaaa
ctgcagatccaaccacttcattcgatgacaaagaaaagaaggaacaatggtcgtgccaaa
aagggccgcggccacgtgcagcctattcgctgcactaactgtgcccgatgcgtgcccaag
gacaaggccattaagacattcgtcattcgaaacatagtgaaggccgcagcagtcagggac
atttctgaagcgagcgtcttcgatgcctatgtgcttcccaagctgtatgtgaagctacat
tactgtgtgagttgtgcaattcacagcaaagtagtcaggaatcgatctcgtgaagcccgc
aaggaccgaacacccccatcccgatttagacctgcgggtgctgccccacgtcccccacca
aagcccatgaatccaacttacaagggatgtgaggacctcttcaaggagaactacaaacca
ctgctcaatgaaataaaagaggacaggaacaaatggaagaacattccatgcttatggata
ggaagaatcaatatcatgaaaatggccatactgcccaagggaacgctcacggactgtgtg
gtcctgagagatccaaacaccaagtgctccgggggctttgggtttgtcacatatgccact
gtggaggaggtggatgcagccacaaatgcaaggccacacaagactgtcattcagaaatac
tatactgtgaatggccacaactgtgaagttaggaaagccctgtcaaagcaagagatggct
agtgtttcatccagccaaagaggtgaagtggttctggaaactttggtggtggtcgtggag
gtggtttcagtgggaatgacaactttggttgtggaggaaactttagtggtcatggtggct
ttggtggcagctgtgatgtcagtggatatggtggcagtggggatggttataatgggtttg
gcaattatggtggttatggaggaagtggccctggttactctggaggaagcagaggctatg
gaagtggtggacagggttatggaaaccagggcagtggctatggcgggagtggcagctatg
acagctataacaatagaggtggaggtagctttggcagtggcagtggaagcagttttggag
gtggtggaagctacagtgatttgggcaattaaaacaatcagttttcaaattttggaccca
tga

>gi568815597r:57956136_58156480|GENSCAN_predicted_peptide_5|189_aa
MKGVTNPAEEGFSDVICTILGEHTVWMGKIQNPCEEEIGTLPPSKENAEISNLQLPLGMP
AGPREGERRIQEGPGKLPERMSFEAVACKCGLANGKGRDMCPGTAISVQSPVQVEAQSTE
SDSWPWGRSEFDQEAAEYAIGKADRQCRGRRKQNNMMMKRKWLAVANQDSNSSSAIHLLT
ALVKITQPL

>gi568815597r:57956136_58156480|GENSCAN_predicted_CDS_5|570_bp
atgaagggtgttactaatccagctgaggaaggattctctgatgtgatctgtactatcctg
ggagaacacacagtatggatggggaagatccagaatccatgtgaagaagaaataggaact
ctacctccatcaaaggagaatgctgagattagcaatttacaactacctttgggcatgcct
gctggccccagggaaggagagaggagaatccaagaaggtcctggaaagttaccagaaagg
atgagtttcgaggcagttgcatgtaaatgtggcttagccaatgggaaaggaagggatatg
tgtcctggtacagcaatatctgttcaatctcctgtacaagtggaggcccagagcactgaa
tctgacagctggccatgggggcgtagtgaatttgaccaagaggcagctgagtatgcaatc
gggaaagctgatagacagtgtaggggcagaaggaagcagaacaacatgatgatgaagaga
aagtggcttgcagttgctaaccaggattcaaactccagctctgccattcatttactgaca
gccttggtcaaaatcacccagcctctttaa

>gi568815597r:57956136_58156480|GENSCAN_predicted_peptide_6|153_aa
MALGNRSYFYPHFTNVETEAQRLSHMPKVTQLASDGAEFTPRPSALTHCTDCYKKDTTSA
LMELPSNGKYKSIQDLSRKRMAHSNWVTRREFNRRPIYKAKQNNAEVQEASSGTEPGGEE
ETEAMEGQMEEIQNNSTQAPQKWVFQDVLEQYQ

>gi568815597r:57956136_58156480|GENSCAN_predicted_CDS_6|462_bp
atggccctaggaaataggtcctatttttacccccacttcacaaatgtggaaactgaggca
cagagactgagtcatatgcccaaggtcacacagctggcaagtgatggagcagaattcaca
ccccggccatctgctcttacacactgtactgactgttacaagaaagacacaacctctgcc
ctcatggagcttccatctaatgggaaatacaaaagcattcaggatctcagcaggaaacgg
atggcacactcaaattgggtaacacgaagggagtttaacagaaggcctatttacaaagcc
aagcaaaataatgcagaagtccaggaagcctccagtggcacagagccaggtggagaagaa
gagacagaggccatggaggggcaaatggaagagatccagaacaactctacccaggcccct
cagaaatgggtgtttcaagatgtcctagagcagtatcagtaa

>gi568815597r:57956136_58156480|GENSCAN_predicted_peptide_7|123_aa
MGKTWEENEWGNLKDPQFVQGDWTMDSKRSREKVAVREELMSLAAHSTGEKLRLHSESKG
EPWRDFQQKNLWVQATETSTNRLKQVAGFARSLRVALEWIRELNNRTSGKKEPGGSRDLG
EEN

>gi568815597r:57956136_58156480|GENSCAN_predicted_CDS_7|372_bp
atgggcaaaacctgggaggaaaatgaatggggaaatctgaaggacccacagtttgttcag
ggggactggacaatggacagcaagagaagtagagagaaagttgctgtgagagaggaactc
atgagtctggctgcacacagcactggtgagaaattgagacttcattctgagagcaagggg
gagccctggcgggattttcagcagaagaatctttgggtgcaagcaacagaaaccagcacc
aacagacttaaacaagtagcaggatttgctagatcactcagggtagctctagaatggatc
agagaattgaataatcggacttcaggcaagaaagagccaggaggctctagggatcttggt
gaggagaactaa

>gi568815597r:57956136_58156480|GENSCAN_predicted_peptide_8|756_aa
MKAEIKMFFETNENKDTTYQNLWDSFKAVCRGKFIALNAHKRKQERSKIDTVTSQLKELE
KQEQTHSKASRRQEITKIRAELKEIETQKTLQKINESRSWFFERINKIDRPLARLIKKKR
EKNQIDTIKNDKGDITTDPTEIQTAIREYYKHLYANKLENLEEMDKFLDTYSLPRLNQEE
VESLNRPITGSEIVAIINSLPTKKSPGPDGFTAEFYQRYKEELVPFLLKLFQSIEKEGIL
PNSFYEASIILIPKPGRDTTKKENFRPISLMNIDAKILNKILAKQIQQHIKKLIHHDQVG
FIPGMQGWFNIRKSINVIQHINRAKDKNHMIISIDAEKAFDKIQQPFMLKTLNKLGIDGT
YLKIIRAIYDKPTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLDVLARAIRQKEIK
GIQLGKEEVKLSLFADDMIVYLENPIVSAQNLLKLISNFSKVSGYKINVQKSQAFLYTNN
RQTESQIMSEFPFTIASKRIKYLGIQLTRDMKDLFKENCKPLLQEIKEDTNKWKNIPCSW
VGRINIVKMAILPKMLPVLQGPSILSKHCTNFLASNLPLFELPLSFLQQHSLLRECVNYH
NRVIEVSSSFTKTLQGFQYTNTCPHSYTHSHYTEVFFAICASAFDNRYFYDGRVEDAAEK
LELGGIRSLLMANDTGLCQSVEEFGKDTIHLGITSHQVAQTRILGTIFNMSLSHAPSNQS
TARSCLARILPIISSPCKILLILHSVWASCLPGSIS

>gi568815597r:57956136_58156480|GENSCAN_predicted_CDS_8|2271_bp
atgaaggcagaaataaagatgttctttgaaaccaacgagaacaaagacacaacataccag
aatctctgggactcattcaaagcagtgtgtagagggaaatttatagcactaaatgcccac
aagagaaagcaggaaagatccaaaattgacaccgtaacatcacaattaaaagaactagaa
aagcaagagcaaacacattcaaaagctagcagaaggcaagaaataactaaaatcagagca
gaactgaaggaaatagagacacaaaaaacccttcaaaaaattaatgaatccaggagctgg
ttttttgaaaggatcaacaaaattgatagaccgctagcaagactaataaagaaaaaaaga
gagaagaatcaaatagacacaataaaaaatgataaaggggatatcaccaccgatcccaca
gaaatacaaactgccatcagggaatactacaaacacctctacgcaaataaactagaaaat
ctagaagaaatggataaattcctggacacatacagtctcccaagactaaaccaggaagaa
gttgaatccctgaatagaccaataacaggatctgaaattgtggcaataatcaatagctta
ccaaccaaaaagagtccaggaccagatggattcacagccgaattctaccagaggtacaag
gaggaactggtaccattccttctgaaactattccaatcaatagaaaaagagggaatcctc
cctaactcattttatgaggccagcatcattctgataccaaagccgggcagagacacaacc
aaaaaagagaattttagaccaatatccttgatgaacattgatgcaaaaatcctcaataaa
atactagcaaaacaaatccagcagcacatcaaaaagcttatccaccatgatcaagtgggc
ttcatccctgggatgcaaggctggttcaatatacgcaaatcaataaatgtaatccagcat
ataaacagagccaaagacaaaaaccacatgattatctcaatagatgcagaaaaagccttt
gacaaaattcaacaacccttcatgctaaaaactctcaataaattaggtattgatgggacg
tatttaaaaataataagagctatctatgacaaacccacagccaatatcatactgaatggg
caaaaactggaagcattccctttgaaaactggcacaagacagggatgccctctctcacca
ctcctattcaacatagtgttggacgttctggccagggcaattaggcagaaggaaataaag
ggtattcaattaggaaaagaggaagtcaaattgtccctgtttgcagacgacatgattgta
tatctagaaaaccccattgtctcagcccaaaatctccttaagctgataagcaacttcagc
aaagtctcaggatacaaaatcaatgtacaaaaatcacaggcattcttatacaccaacaac
agacaaacagagagccaaatcatgagtgaattcccattcacaattgcgtcaaagagaata
aaatacctaggaatccaacttacaagggatatgaaggacctcttcaaggagaactgcaaa
ccactgctccaggaaataaaagaggatacaaacaaatggaagaacattccatgctcatgg
gtaggaagaatcaatatcgtgaaaatggccatactgcccaagatgctccccgtacttcaa
ggaccttctatactttccaaacactgcacaaatttcctggcatcaaatctgcccctattt
gagctccccttatctttcctgcagcagcacagtctgcttagggagtgtgttaattaccac
aatagggtcattgaagttagctcttcgtttacaaaaaccctccagggattccagtacaca
aacacatgcccacactcatacacacactctcattacacagaagttttttttgccatttgt
gcttcagcttttgacaacagatacttttatgatggcagagtggaagatgcagctgaaaag
ctggaacttggaggaatccgaagtttgctaatggcaaatgatacaggcctgtgtcagtca
gtggaggaattcgggaaggacaccattcacctgggcatcacgagccaccaggttgcccaa
accagaatccttggtaccatctttaacatgtccctctcccacgcaccctccaaccaatca
acagctcggtcctgtctggccagaatcctgcccatcatctcatctccttgcaaaatcctc
ctcattctccactcagtctgggcatcatgtcttccaggaagcatttcctga

>gi568815597r:57956136_58156480|GENSCAN_predicted_peptide_9|258_aa
MGKKQNRKTGNSKKQSASPPPKERSSSPAMEQSWMENDFDELREEGFRRSNYSELWEDIQ
TKGNEVENFEKNLEECITRITNREKCLKELMELKTKARELREECRSLRSRCDQLEERVSA
MEDEMNEMKREGKSREKRIKRNEQSLQEIWDYVKRPNLRLIGVPESDGENGTKLENTLQD
IIQENFPNLARQANVQIQEIQRTPQRYSSRRATPRHIIVRFTKVEMKEKMLRAAREKGRV
TLKGKPIRLTADLSAETL

>gi568815597r:57956136_58156480|GENSCAN_predicted_CDS_9|777_bp
atggggaaaaaacagaacagaaaaactggaaactctaaaaagcagagcgcctctcctcct
ccaaaggaacgcagttcctcaccagcaatggaacaaagctggatggagaatgactttgac
gagctgagagaagaaggcttcagacgatcaaattactctgagctatgggaggacattcaa
accaaaggcaatgaagttgaaaactttgaaaaaaatttagaagaatgtataactagaata
accaatagagagaagtgcttaaaggagctgatggagctgaaaaccaaggctcgagaacta
cgtgaagaatgcagaagcctcaggagccgatgcgatcaactggaagaaagggtatcagca
atggaagatgaaatgaatgaaatgaagcgagaagggaagtctagagaaaaaagaataaaa
agaaatgagcaaagcctccaagaaatatgggactatgtgaaaagaccaaatctacgtctg
attggtgtacctgaaagtgatggggagaatggaaccaagttggaaaacactctgcaggat
attatccaggagaacttccccaatctagcaaggcaggccaacgttcagattcaggaaata
cagagaacgccacaaagatactcctcgagaagagcaactccaagacacataattgtcaga
ttcaccaaagtggaaatgaaggaaaaaatgttaagggcagccagagagaaaggtcgggtt
accctcaaagggaagcccatcagactaacggcggatctctcggcagaaaccctataa

>gi568815597r:57956136_58156480|GENSCAN_predicted_peptide_10|132_aa
XLFGKSNKMKDVEVLCKSVWLKSGTPEGTPRISISNKCPGEADATGLGTTLRAIALENLW
HKWMQQEGTILEADSKPSPDAKSADALILDFPTFRTLCEEKLHSLMPPSWLSFCNTWLPS
VNDQRTLGNFTE

>gi568815597r:57956136_58156480|GENSCAN_predicted_CDS_10|399_bp
nggctttttggaaaatcaaataagatgaaagatgtggaagtactttgtaaatcagtctgg
ttgaagagtggcactccagaaggaacaccgagaatttccatttccaataagtgcccaggt
gaagctgatgctacaggtctagggacaactttgagagccattgccttagagaacttatgg
cacaaatggatgcagcaagaaggcaccatcttggaagcagacagcaagccttcaccagat
gccaaatctgctgatgctttgatcttagacttcccaacgttcagaactctctgtgaagaa
aaattgcattcactgatgccaccaagctggctgtccttctgcaatacctggctgccaagt
gtaaatgatcaaagaacacttggaaacttcacagagtga