GENSCAN 1.0	Date run:  8-Nov-116	Time: 13:10:26

Sequence gi568815591f:151867247_152119798 : 252552 bp : 43.71% C+G : Isochore 2 (43 - 51 C+G%)

Parameter matrix: HumanIso.smat

Predicted genes/exons:

Gn.Ex Type S .Begin ...End .Len Fr Ph I/Ac Do/T CodRg P.... Tscr..
----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------

 1.01 Intr +    652    831  180  1  0  111   71    53 0.687   4.88
 1.02 Term +   2437   2617  181  1  1   75   49   147 0.433   6.58
 1.03 PlyA +   7483   7488    6                               1.05

 2.03 PlyA -   7690   7685    6                               1.05
 2.02 Term -   8153   8130   24  2  0   60   52    -2 0.160  -8.38
 2.01 Init -   9359   9261   99  2  0   68  117   152 0.661  16.36
 2.00 Prom -  14501  14462   40                              -5.46

 3.00 Prom +  14885  14924   40                              -3.16
 3.01 Init +  15884  15942   59  1  2   65   98   114 0.734  10.88
 3.02 Intr +  18076  18174   99  1  0   49   72    65 0.053   0.23
 3.03 Intr +  26449  26598  150  1  0   77   17   104 0.065   1.48
 3.04 Term +  34854  34938   85  0  1   95   43    92 0.187   2.53
 3.05 PlyA +  35818  35823    6                               1.05

 4.00 Prom +  46428  46467   40                              -2.36
 4.01 Init +  52397  52638  242  1  2   68   94    48 0.005   0.75
 4.02 Intr +  63648  63753  106  0  1   77   37    73 0.003   1.32
 4.03 Intr +  68932  69046  115  0  1   94   60    76 0.039   5.42
 4.04 Intr +  77048  77084   37  0  1  107  103    10 0.071   1.72
 4.05 Intr +  83174  83284  111  2  0   73   52   111 0.531   5.49
 4.06 Intr + 115740 115906  167  0  2  109   91    50 0.280   7.00
 4.07 Intr + 135468 135622  155  1  2  111   61   198 0.536  19.19
 4.08 Intr + 138160 138317  158  0  2   90   50    51 0.303   0.31
 4.09 Intr + 140581 140698  118  1  1  121   53    33 0.390   3.57
 4.10 Intr + 147398 147547  150  1  0   71   81    25 0.126   0.46
 4.11 Term + 152400 152555  156  2  0   73   48   121 0.943   4.53
 4.12 PlyA + 152579 152584    6                               1.05

 5.00 Prom + 156707 156746   40                              -2.66
 5.01 Init + 158587 158638   52  0  1   81  101    58 0.704   7.93
 5.02 Term + 161450 161484   35  0  2  110   42     9 0.171  -3.75
 5.03 PlyA + 162866 162871    6                               1.05

 6.05 PlyA - 163845 163840    6                               1.05
 6.04 Term - 165192 164621  572  1  2   34   42   172 0.362   1.80
 6.03 Intr - 166174 165904  271  1  1   63   32   159 0.591   4.91
 6.02 Intr - 167541 167427  115  2  1   63  109    40 0.870   4.05
 6.01 Init - 168779 168574  206  2  2   47   73   120 0.747   4.72
 6.00 Prom - 171039 171000   40                              -5.16

 7.04 PlyA - 171695 171690    6                               1.05
 7.03 Term - 172512 172214  299  1  2   52   47   226 0.757  10.33
 7.02 Intr - 173936 173577  360  0  0   48   98   236 0.867  15.79
 7.01 Init - 176652 176544  109  0  1   72   98    43 0.546   4.08
 7.00 Prom - 181707 181668   40                              -0.36

 8.05 PlyA - 183072 183067    6                               1.05
 8.04 Term - 183642 183514  129  0  0   39   37   114 0.409  -0.42
 8.03 Intr - 185454 185293  162  0  0   38  102    60 0.328   2.57
 8.02 Intr - 191790 191711   80  1  2   43   45    66 0.239  -2.93
 8.01 Init - 193588 193456  133  1  1   78   47    72 0.222   2.40
 8.00 Prom - 193712 193673   40                              -1.56

 9.02 PlyA - 194476 194471    6                               1.05
 9.01 Sngl - 195977 195531  447  2  0   70   48   173 0.879   7.73
 9.00 Prom - 196921 196882   40                              -4.96

10.03 PlyA - 197086 197081    6                               1.05
10.02 Term - 198251 198078  174  2  0   65   44   144 0.880   5.46
10.01 Init - 201058 200993   66  1  0   38   93    45 0.582   1.07
10.00 Prom - 214612 214573   40                              -3.26

11.00 Prom + 214776 214815   40                              -5.66
11.01 Init + 222661 222731   71  0  2   61   64    32 0.284  -1.38
11.02 Intr + 227074 227276  203  1  2   64   97   149 0.628  12.33
11.03 Intr + 233552 233675  124  0  1  111   41    60 0.993   3.24
11.04 Intr + 235866 236032  167  0  2   91   87   126 0.993  12.40
11.05 Intr + 237999 238124  126  1  0   64   69    37 0.462   0.05
11.06 Intr + 240792 240957  166  1  1  134  -12   367 0.550  30.32
11.07 Intr + 243282 243399  118  0  1   78   58    69 0.971   3.37
11.08 Intr + 246000 246152  153  2  0   82  100   117 0.977  12.57
11.09 Intr + 249911 250129  219  1  0   58   90    96 0.972   5.30
11.10 Term + 251432 251650  219  1  0   81   40   185 0.691  10.04
11.11 PlyA + 251919 251924    6                               1.05


Suboptimal exons with probability > 0.800

Exnum Type S .Begin ...End .Len Fr Ph B/Ac Do/T CodRg P.... Tscr..
----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------



Predicted peptide sequence(s):

Predicted coding sequence(s):


>gi568815591f:151867247_152119798|GENSCAN_predicted_peptide_1|120_aa
XTVEVTAGPPPAMNSPGRRPAELQGTPLQDQAFGSWKRRWEPGVTEQTGLCRAFISSFTA
RTSTGPCSSGIGPMCRAPLRTTLADQPSHTQRAAERQPSFIKNPGATGQSEDPITKKLDY

>gi568815591f:151867247_152119798|GENSCAN_predicted_CDS_1|363_bp
nncacagtggaggtcactgcagggccacccccagcaatgaactcccctggaagaaggcca
gcagagctgcagggcacccccctccaggaccaggcctttggctcatggaaaagacgctgg
gagcctggggttacggagcaaactggtctttgtcgtgcattcatcagcagcttcactgcc
aggaccagcactgggccgtgctcaagcggcattgggccaatgtgccgtgccccactgagg
acgaccctcgctgatcagccctctcacacccagcgtgctgcagagcggcaaccaagcttc
atcaaaaaccctggagccacaggccagtctgaggaccccatcaccaagaagcttgactac
tga

>gi568815591f:151867247_152119798|GENSCAN_predicted_peptide_2|40_aa
MDTKKKKDVSSPGGSGGKKNASQKRRSLRVHIPGALPRVC

>gi568815591f:151867247_152119798|GENSCAN_predicted_CDS_2|123_bp
atggacaccaagaagaaaaaagatgtttccagccccggcgggagcggcggcaagaaaaat
gccagccagaagaggcgttcgctgcgcgtgcacattccgggcgcactgccccgtgtttgc
tga

>gi568815591f:151867247_152119798|GENSCAN_predicted_peptide_3|130_aa
MPVNHKEVLIVKSKKYFGLSLTLPQWAQRLPGSLDCGPRVTEYSSRPLTDMVGWGECEGP
KRGSGLSVKDARPPSACIVILPRDGCDPPLRHPPPPHHQGCGSRTKKKGLNTKEMSCIEV
EELTDEQRKN

>gi568815591f:151867247_152119798|GENSCAN_predicted_CDS_3|393_bp
atgccagtcaaccataaggaagtcctcatcgtgaaatccaagaagtactttggattaagc
ctgaccctccctcagtgggcccagcgtctgccgggcagccttgactgtggccctagagtc
acggaatactcgagcagacctctgacggacatggtggggtggggagagtgtgaaggaccc
aagagaggttctgggctgtcagtcaaggatgccagacccccaagtgcctgcattgtgatt
ctcccccgggatggctgtgaccccccgctgcgtcaccccccacctccacaccaccaaggc
tgtggcagccgaacaaagaagaagggcttgaacactaaagaaatgtcatgcattgaggta
gaagaactgactgatgaacagaggaaaaactga

>gi568815591f:151867247_152119798|GENSCAN_predicted_peptide_4|504_aa
MGPQNQLNPGKSQREGQAGSGESRKQQYRAAVLWQVHAFHPWGQERPTPISSAEQPPQQL
HVGCPVCEESKQKLGHRKLSRHYQSITALFTHSQGLQTSLEKDSWKQEWSAASDAAELSN
AKLNGPSMPSGSERLVELKMKSARVFVLFRLRHRAPQPQPRAHALRWAPIKINWTVANDS
NCLKLNQVVAPVVDVLSSVGFGTWCLQKHYPARLPTASIVICFYNEECNALFQTMSSVTN
LTPHYFLEEIILVDDMSKVGDVLVFLDSHCEVNRVWLEPLLHAIAKDPKMVVCPLIDVID
DRTLEYKPSPLTTADSNVFGLHTKWTELQCPEMRNTMSGAGFWQEDSLWGIFEMSIWHSS
GDVKSPAMSGGIFAIRRHYFNEIGQYDKDMDFWGRENLELSLRIWMCGGQLFIIPCSRVG
HISKKQTGKPSTIISAMTHNYLRLVHVWLDEYKEQFFLRKPGLKYVTYGNIRERVELRKR
LGCKSFQWYLDNVFPELEASVNSL

>gi568815591f:151867247_152119798|GENSCAN_predicted_CDS_4|1515_bp
atgggccctcagaaccagcttaacccaggcaagagtcagcgggaaggccaggctggtagt
ggggagagcaggaagcagcagtacagggccgctgtcctttggcaagtacatgcctttcac
ccctggggtcaggagaggcccactccgatttcctccgcagagcagccaccgcagcagctg
catgtgggatgccctgtatgtgaggagtccaagcagaagctgggacacaggaagttgtcc
aggcattatcaatcaatcacagcactcttcacgcacagccaagggctgcagacatcactg
gaaaaggattcctggaagcaggaatggtctgcagcgtcagatgccgcggaactaagtaat
gcaaaactcaacgggcctagtatgccatctggtagtgaacgtctagttgaattaaagatg
aaaagtgctagggtgtttgtattgtttcgactaagacacagagccccacagccccagccc
cgggcccacgctttgagatgggcccctatcaaaatcaactggaccgtagcaaatgacagt
aactgtcttaaacttaatcaagtggtagccccagtagtagatgtgctgtcttcagtgggc
tttggcacttggtgtcttcaaaaacattacccagcccgcctcccgactgccagcattgtc
atttgcttctataatgaagaatgtaatgccttgtttcagaccatgtccagtgtcacgaac
ctcacgccacactattttcttgaagaaattattttggtagatgacatgagcaaagttggg
gatgttctggtgttcctggacagccactgtgaggtgaacagagtatggctggagcccctg
ctgcatgccattgccaaggaccccaaaatggtggtgtgccccctgatagatgtcattgat
gatagaactctggagtataagccctctcctcttaccacagctgactccaacgttttcggc
ctgcacaccaaatggacggaactgcagtgccctgagatgagaaataccatgagtggagca
ggtttttggcaggaagattctctttggggcatatttgagatgtctatttggcattcaagt
ggggatgtgaagtcacctgcaatgtctggaggaatttttgctatacgtcggcattatttt
aatgaaattggacagtatgacaaggatatggatttttggggaagagaaaatttggaactt
tcactaaggatctggatgtgtggaggccaactctttataatcccctgctctcgagtagga
catatcagtaagaaacaaactggaaaaccttctacaatcatcagtgctatgacacataac
tacctaagactggtgcacgtttggctggatgaatataaggagcagttttttcttcgaaag
cctggtctgaaatatgtcacctacggaaatattcgcgagcgtgttgagttaaggaaacga
ctgggttgcaagtcatttcagtggtatttggataatgtcttcccagagttggaggcatct
gtgaacagcctgtga

>gi568815591f:151867247_152119798|GENSCAN_predicted_peptide_5|28_aa
MPQPESQKAAILGCGQGGHPNCCWEFGQ

>gi568815591f:151867247_152119798|GENSCAN_predicted_CDS_5|87_bp
atgccgcagcccgagtcccagaaggcggcgatcctgggctgcgggcaaggcggacacccc
aactgctgttgggaatttggccaatga

>gi568815591f:151867247_152119798|GENSCAN_predicted_peptide_6|387_aa
MRKDSCASSMHQQVSRSKKRAGQKTPPEDQEGGQRALRSSHIRLGQFLLIEDCKTPSPSS
LGADAIAKQRKTSVSAAASVSATIPIRRVQGPTVVGSWARGVSAAASGPRGTGPKGKARS
EKGCSLSHGPQTNKPLVVQKGQKMEQANHPVGLVISVVYKDILKKIVQRETSHPLIHVRY
AEAITGRRTAPEDKGSLVVPNPYTLLSQIPEEAEWFPVLDLKDAFFCIPLHYDSHDSQFL
FAFEDPTDHTSQLIWTVLPQGFRDSPHLFGQALAQDLGHFSSPGTLVLQYVDDLLLATSS
EASCQQATLDLLNFLANQGYKASRSKAQLCLQQVKYLGLILARGTRTLGKERIQPILAYP
HPKTLKQLWGFLQITAFANYGSPDRAR

>gi568815591f:151867247_152119798|GENSCAN_predicted_CDS_6|1164_bp
atgcgcaaggacagttgcgccagcagcatgcatcagcaagttagcagaagcaagaagaga
gctgggcagaagacaccccctgaagatcaagagggaggccagcgggcactacgcagcagc
cacatcagactgggacaattcctgcttatagaggactgtaaaaccccttccccatcctca
cttggggctgacgctattgccaagcagaggaaaactagtgtttctgctgctgcgtcagtg
agtgcaactattcccatccgcagggtccagggaccgaccgttgtgggttcttgggcaaga
ggtgtttctgctgctgcatcgggaccaagaggaacaggcccaaaaggaaaagcgagatca
gagaaaggctgcagccttagtcatggccctcagacaaacaaacctttggtggttcagaaa
ggacagaaaatggagcaggccaatcacccggtagggcttgttatcagtgtggtttacaaa
gacattttaaaaaagattgtccaacgagaaacaagccaccccctcatccatgtccgctat
gccgaggcaatcactggaaggcgcactgccccggaggacaaaggttctctggttgtaccc
aacccctataccctgctctctcaaataccagaggaagcagaatggttccctgttctggac
ctcaaggatgccttcttctgtattcccctgcactatgactcccatgactcccagtttctc
tttgcctttgaggatcccacagaccacacatcccaacttatatggaccgtcttgccccaa
gggtttagggatagccctcatctgtttggtcaggcactggcccaagatctaggccacttc
tcaagtccaggcactctggtccttcagtatgtggatgatttacttttggctaccagttcg
gaagcctcatgccagcaggctactctagatctcttgaattttctagctaatcaagggtac
aaggcatctaggtcaaaggcccagctttgcctacagcaggtcaaatatctaggcctaatc
ttagccagagggaccaggacccttggcaaggaacgaatacagcctatactggcttatcct
caccctaagactttaaaacagttgtgggggttccttcaaatcaccgcttttgccaactat
ggatccccagatagagcaagatag

>gi568815591f:151867247_152119798|GENSCAN_predicted_peptide_7|255_aa
MGQVWALVHSTLETFHTDEEEGEYNEVTEQVCLPAKGPNQQPIWIPSRYLKPYHKPDAKE
EIPEGSQGFPVAAMSRLTLRRTPTVTSNTHRTQPPTWGQIEKLPQMAEENLRKAGQPVTI
SNWILPRITKFKPIEGAENVFTDGSSNGKASYSGSKGPNQQPIWIPSKHLKPYHKPDAGE
KIPGESRGPPVAAMSRLTLRRMPTVMSNTHRTQPPTWGQIKKLSQMAEENLRKAGQPVTM
NNLMIAVITTAFNKG

>gi568815591f:151867247_152119798|GENSCAN_predicted_CDS_7|768_bp
atgggacaagtgtgggctctggttcattccaccttggaaacttttcacactgatgaggag
gaaggagagtataatgaagtaacagagcaggtttgtttgccagctaaaggaccaaatcaa
cagccaatttggataccatcaagatacctgaaaccttatcataagccagatgccaaggaa
gagattccggaaggatcccaaggattcccagttgcagccatgtcaagactgacgctgagg
aggaccccaactgtcacgagcaacacccatcgaacacagccacccacctggggacagatc
gagaagctgccacagatggcggaagaaaacctgaggaaagctggacaaccagtcacaata
agtaattggattctccctagaataactaaatttaaaccaattgaaggtgctgagaatgtt
tttacagatgggtctagcaacggtaaagcttcttattctggctcaaaaggaccaaatcaa
cagccgatttggataccatcaaaacacctgaaaccttatcataagccagatgctggggaa
aagattccaggagaatcccgaggacccccggttgcagccatgtcaagactgacgctgagg
aggatgccaactgtcatgagcaacacccatcgaacacagccacccacctggggacagatc
aagaagctgtcacagatggcggaagaaaacctgaggaaagcgggacaaccagtcacaatg
aataatttaatgatagcggtgatcaccactgccttcaacaagggctga

>gi568815591f:151867247_152119798|GENSCAN_predicted_peptide_8|167_aa
MEYYTAIEKGEFMSFVGTWMKLETIILSKLLQGQKTKHRMFSLIELQNVNINGSLEEVGS
NLDRQVQDFSGDCVEEEEPRKGLRRITKQKNKRVYRQQCEVPQRFIEKLRQVRREVEPSD
LATSKWKAKGGRIQLAPTEEAFRAVARGELPILVVETQVPARLTTMG

>gi568815591f:151867247_152119798|GENSCAN_predicted_CDS_8|504_bp
atggaatactatacagccatagaaaagggtgagttcatgtcctttgtagggacatggatg
aagctggaaaccatcattctcagcaaactactgcaaggacaaaaaaccaaacaccgcatg
ttctcactcatagaactacaaaatgtcaatattaacgggagtttagaagaagttggttcc
aaccttgaccgacaggttcaagacttcagtggagactgtgtggaggaggaagaacccagg
aagggcctgagaaggattacaaagcagaagaataagagggtctatcgacagcaatgtgag
gtgccacaaagatttatagagaagctaagacaggtgagaagagaggtagagccatcagat
ttagcaactagtaagtggaaagcaaaagggggcagaattcagctggcgcccacagaggaa
gcatttagagctgtagccagaggggaattgcccatcctggtggtggaaactcaagttcca
gcaagacttaccaccatgggctaa

>gi568815591f:151867247_152119798|GENSCAN_predicted_peptide_9|148_aa
MDKFLDTYTLPRLNQEEVESLNRKITGSEIEAILNSLPTKKSPGPDGFTVEFYQKYKEEL
VPFLLKLFLSIEKEGILPNSFYEASIILIPKLGRDTTKKENFRPISLMNTDAKILNKILA
YQIQQHIKKLIHHDQVGFIPGMQGWFNI

>gi568815591f:151867247_152119798|GENSCAN_predicted_CDS_9|447_bp
atggataaattcctggacacatacaccctcccaagactaaaccaggaagaagttgaatcc
ctgaataggaaaataacaggctctgaaattgaggcaatacttaatagcctaccaacaaaa
aaaagtccaggaccagatggattcacagttgaattctaccagaagtacaaggaggagctg
gtaccattccttctgaaactattcctatcaatagaaaaagagggaatcctccctaactca
ttttatgaagccagcatcatcctgataccaaagcttggcagagacacaacaaaaaaagag
aattttagaccaatatccctgatgaacaccgatgcaaaaatcctcaataaaatacttgca
taccaaatccagcagcacatcaaaaagcttatccaccatgatcaagtgggcttcatccct
gggatgcaaggctggttcaacatatga

>gi568815591f:151867247_152119798|GENSCAN_predicted_peptide_10|79_aa
MKDANLKRLHIECFPLYDILEKECSSSPAIEQSWMENDFDELREGFRRSNYSELKEEVRM
HRKEAKNLEKRLDKWLTRM

>gi568815591f:151867247_152119798|GENSCAN_predicted_CDS_10|240_bp
atgaaggacgccaacttgaaaaggctacatattgaatgctttccattgtatgacattctg
gaaaaggaatgcagttcctcaccagcaatagaacaaagctggatggagaatgactttgac
gagttgagagaagggttcagacgatcaaactactccgagctaaaggaggaagttcgaatg
catcgcaaagaagctaaaaaccttgaaaaaagattagacaaatggctaactagaatgtag

>gi568815591f:151867247_152119798|GENSCAN_predicted_peptide_11|521_aa
MMLSIGTIEEVMNFATSGHMTPEHEVTQPLKNVPVKGSGPHGPSPKKFYPRFTRGPSRVL
EPQFKANKIDDVIDSRVEDPEEGHLKFSSELGMIFNERDQELRDLGYQKHAFNMLISDRL
GYHRDVPDTRNAACKEKFYPPDLPAASVVICFYNEAFSALLRTVHSVIDRTPAHLLHEII
LVDDDSDFDDLKGELDEYVQKYLPGKIKVIRNTKREGLIRGRMIGAAHATGEVLVFLDSH
CEVNVMWLQPLLAAIREDRHTVVCPVIDIISADTLAYSSSPVVRGGSPTMAGGLFAMNRQ
YFHELGQYDSGMDIWGGENLEISFRIWMCGGKLFIIPCSRVGHIFRKRRPYGSPEGQDTM
THNSLRLAHVWLDEYKEQYFSLRPDLKTKSYGNISERVELRKKLGCKSFKWYLDNVYPEM
QISGSHAKPQQPIFVNRGPKRPKVLQRGRLYHLQTNKCLVAQGRPSQKGGLVVLKACDYS
DPNQVSDTSGLTASVRKEASSVGKLLPVTPEDISVYYSGFS

>gi568815591f:151867247_152119798|GENSCAN_predicted_CDS_11|1566_bp
atgatgttatctataggaacaatcgaggaagtcatgaattttgcgacgtctggccacatg
actcccgagcatgaagtgactcagccacttaagaatgtgcccgtcaaggggtctgggccc
cacggaccatctccaaaaaaattctatccccgtttcactcgaggcccaagtcgagtgctc
gagccacagttcaaagcaaacaaaattgacgatgtgatagacagtcgtgttgaagatcca
gaagaaggccacttgaaattctcttctgaattaggtatgatttttaatgaacgcgatcaa
gagttgagagacttgggctatcagaaacatgcttttaatatgcttatcagtgaccgcttg
ggctaccacagagatgtgccagacacaaggaatgcagcatgtaaagaaaagttctaccca
cctgacctgccagctgctagtgttgttatctgtttctataatgaagcgttttctgccttg
cttcggacagtgcacagtgtcatagaccgcacgccagcacacctgcttcatgagatcatc
cttgtggatgatgatagtgactttgatgatttgaaaggagaactagatgaatatgtccaa
aaatacctccctggaaaaattaaagtcataagaaatacaaagcgtgaggggttgattcga
gggagaatgattggcgcggcccacgcgacaggagaagtccttgtgttcctggacagccac
tgtgaagtgaatgtgatgtggctgcagcccttgctggccgccatccgtgaggaccggcac
accgtggtgtgcccagtgattgacatcatcagcgccgacacgctggcctacagctcgtcc
cctgtcgtccgcggagggtcaccaacaatggctggaggtttgtttgccatgaacagacag
tatttccatgaacttggacagtatgatagtggcatggatatctggggaggagaaaatttg
gaaatatcatttcggatctggatgtgtggcggtaagctcttcatcatcccttgctctaga
gtaggacacattttccgaaaaaggcgaccatatggatctcccgaaggccaggacaccatg
acacacaactctttgcggctggcacatgtctggttggatgaatacaaggagcagtatttt
tccttaagacctgacctgaagacgaaaagctatggcaatatcagtgagcgtgtggaactg
agaaagaagttgggctgtaaatcatttaaatggtatttggataatgtatacccagagatg
cagatatctgggtcccacgccaaaccccaacaacccatttttgtcaatagagggccaaaa
cgacccaaagtccttcaacgtggaaggctctatcacctccagaccaacaaatgcctggtg
gcccagggccgcccaagtcagaagggaggtctcgtggtgcttaaggcctgtgactacagt
gacccaaatcaggtgagtgacacctcggggctcacagccagtgtccgcaaggaggcttcc
agtgtagggaagctgctgccagtcactcctgaggacatcagtgtctactattctggcttt
tcttaa