Restriction map for
pF1KE9668
(Linear) (Six-base) MAPSORT of: KIEE9668.seq Check: 1487 from: 1 to: 4086
KIEE9668.orf
With 182 enzymes: SgfI *
December 21, 2016 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2495 2522 2763 3443 4086
Size: 2495 27 241 680 643
Fragments arranged by size:
2495 680 643 241 27
AatII G_ACGT'C
Cuts at: 0 1265 4086
Size: 1265 2821
AccI GT'mk_AC
Cuts at: 0 79 4086
Size: 79 4007
Acc65I G'GTAC_C
Cuts at: 0 585 4086
Size: 585 3501
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1951 1996 3357 3742 4086
Size: 1951 45 1361 385 344
Fragments arranged by size:
1951 1361 385 344 45
AfeI AGC'GCT
Cuts at: 0 337 2005 4086
Size: 337 1668 2081
Fragments arranged by size:
2081 1668 337
AgeI A'CCGG_T
Cuts at: 0 3166 4086
Size: 3166 920
AleI CACnn'nnGTG
Cuts at: 0 2410 4086
Size: 2410 1676
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3920 4086
Size: 3920 166
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3583 3615 4086
Size: 3583 32 471
Fragments arranged by size:
3583 471 32
AlwNI CAG_nnn'CTG
Cuts at: 0 738 1530 2802 2907 3234 3285 4086
Size: 738 792 1272 105 327 51 801
Fragments arranged by size:
1272 801 792 738 327 105 51
ApaI G_GGCC'C
Cuts at: 0 3522 3825 4086
Size: 3522 303 261
Fragments arranged by size:
3522 303 261
ApaLI G'TGCA_C
Cuts at: 0 1561 1900 2989 4086
Size: 1561 339 1089 1097
Fragments arranged by size:
1561 1097 1089 339
ApoI r'AATT_y
Cuts at: 0 1935 4041 4086
Size: 1935 2106 45
Fragments arranged by size:
2106 1935 45
AvaI C'yCGr_G
Cuts at: 0 2191 3692 4086
Size: 2191 1501 394
Fragments arranged by size:
2191 1501 394
BanI G'GyrC_C
Cuts at: 0 585 723 2186 2412 3140 3842 4086
Size: 585 138 1463 226 728 702 244
Fragments arranged by size:
1463 728 702 585 244 226 138
BanII G_rGCy'C
Cuts at: 0 938 1524 1541 1925 2116 2429 3371 3398
Size: 938 586 17 384 191 313 942 27
Cuts at: 3398 3522 3601 3825 3943 4086
Size: 124 79 224 118 143
Fragments arranged by size:
942 938 586 384 313 224 191 143
124 118 79 27 17
BbeI G_GCGC'C
Cuts at: 0 3846 4086
Size: 3846 240
BbsI GAAGACnn'nnnn_
Cuts at: 0 853 2150 3559 4011 4086
Size: 853 1297 1409 452 75
Fragments arranged by size:
1409 1297 853 452 75
BbvCI CC'TCA_GC
Cuts at: 0 632 3107 4086
Size: 632 2475 979
Fragments arranged by size:
2475 979 632
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1555 1589 4086
Size: 1555 34 2497
Fragments arranged by size:
2497 1555 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 385 4086
Size: 385 3701
BglI GCCn_nnn'nGGC
Cuts at: 0 171 2613 2655 3444 3724 3820 4086
Size: 171 2442 42 789 280 96 266
Fragments arranged by size:
2442 789 280 266 171 96 42
BlpI GC'TnA_GC
Cuts at: 0 1535 1852 4086
Size: 1535 317 2234
Fragments arranged by size:
2234 1535 317
Bme1580I G_kGCm'C
Cuts at: 0 761 1007 1421 1565 1904 2189 2261 2417
Size: 761 246 414 144 339 285 72 156
Cuts at: 2417 2993 3522 3825 4086
Size: 576 529 303 261
Fragments arranged by size:
761 576 529 414 339 303 285 261
246 156 144 72
BmgBI CAC'GTC
Cuts at: 0 541 1002 1143 3838 4086
Size: 541 461 141 2695 248
Fragments arranged by size:
2695 541 461 248 141
BmrI ACTGGGnnnn_n'
Cuts at: 0 1162 3945 4086
Size: 1162 2783 141
Fragments arranged by size:
2783 1162 141
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 226 936 2145 4086
Size: 226 710 1209 1941
Fragments arranged by size:
1941 1209 710 226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 770 1240 1321 1525 2020 2100 2443
Size: 446 324 470 81 204 495 80 343
Cuts at: 2443 2561 2758 3542 3826 4086
Size: 118 197 784 284 260
Fragments arranged by size:
784 495 470 446 343 324 284 260
204 197 118 81 80
Bpu10I CC'TnA_GC
Cuts at: 0 365 632 1244 2498 2528 3107 3728 4086
Size: 365 267 612 1254 30 579 621 358
Fragments arranged by size:
1254 621 612 579 365 358 267 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 584 1045 1066 2027 4086
Size: 196 388 461 21 961 2059
Fragments arranged by size:
2059 961 461 388 196 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 139 1053 1963 2574 4086
Size: 139 914 910 611 1512
Fragments arranged by size:
1512 914 910 611 139
BsaAI yAC'GTr
Cuts at: 0 1793 4086
Size: 1793 2293
BsaBI GATnn'nnATC
Cuts at: 0 1957 4086
Size: 1957 2129
BsaHI Gr'CG_yC
Cuts at: 0 745 1262 3843 4008 4086
Size: 745 517 2581 165 78
Fragments arranged by size:
2581 745 517 165 78
BsaWI w'CCGG_w
Cuts at: 0 3166 4086
Size: 3166 920
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2681 2711 3583 3613 4086
Size: 2681 30 872 30 473
Fragments arranged by size:
2681 872 473 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 602 635 1399 1467 1525 1528 1694 1852
Size: 602 33 764 68 58 3 166 158
Cuts at: 1852 2064 2121 2564 3349 3630 3718 3795 3844
Size: 212 57 443 785 281 88 77 49
Cuts at: 3844 4086
Size: 242
Fragments arranged by size:
785 764 602 443 281 242 212 166
158 88 77 68 58 57 49 33
3
BseYI C'CCAG_C
Cuts at: 0 104 731 764 794 951 1023 1523 1592
Size: 104 627 33 30 157 72 500 69
Cuts at: 1592 1887 2261 2282 2429 2471 2519 2600 2617
Size: 295 374 21 147 42 48 81 17
Cuts at: 2617 2661 2855 2939 2945 2957 3254 3311 3440
Size: 44 194 84 6 12 297 57 129
Cuts at: 3440 3944 4086
Size: 504 142
Fragments arranged by size:
627 504 500 374 297 295 194 157
147 142 129 104 84 81 72 69
57 48 44 42 33 30 21 17
12 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 2467 2833 2869 2917 3637 4086
Size: 673 1794 366 36 48 720 449
Fragments arranged by size:
1794 720 673 449 366 48 36
BsiEI CG_ry'CG
Cuts at: 0 3391 3448 4086
Size: 3391 57 638
Fragments arranged by size:
3391 638 57
BsiHKAI G_wGCw'C
Cuts at: 0 212 1204 1541 1565 1904 2116 2534 2993
Size: 212 992 337 24 339 212 418 459
Cuts at: 2993 3740 3943 4086
Size: 747 203 143
Fragments arranged by size:
992 747 459 418 339 337 212 212
203 143 24
BsmI GAATG_Cn'
Cuts at: 0 366 4086
Size: 366 3720
Bsp1286I G_dGCh'C
Cuts at: 0 212 761 938 1007 1204 1421 1524 1541
Size: 212 549 177 69 197 217 103 17
Cuts at: 1541 1565 1904 1925 2116 2189 2261 2417 2429
Size: 24 339 21 191 73 72 156 12
Cuts at: 2429 2534 2993 3371 3398 3522 3601 3740 3825
Size: 105 459 378 27 124 79 139 85
Cuts at: 3825 3943 4086
Size: 118 143
Fragments arranged by size:
549 459 378 339 217 212 197 191
177 156 143 139 124 118 105 103
85 79 73 72 69 27 24 21
17 12
BspHI T'CATG_A
Cuts at: 0 512 713 4086
Size: 512 201 3373
Fragments arranged by size:
3373 512 201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 203 2495 2522 2763 2801 3443 4086
Size: 203 2292 27 241 38 642 643
Fragments arranged by size:
2292 643 642 241 203 38 27
BsrBI CCG'CTC
Cuts at: 0 14 784 3691 3759 4086
Size: 14 770 2907 68 327
Fragments arranged by size:
2907 770 327 68 14
BsrDI GCAATG_nn'
Cuts at: 0 1749 2399 4086
Size: 1749 650 1687
Fragments arranged by size:
1749 1687 650
BsrFI r'CCGG_y
Cuts at: 0 974 2068 3166 4086
Size: 974 1094 1098 920
Fragments arranged by size:
1098 1094 974 920
BssSI C'ACGA_G
Cuts at: 0 910 1564 3319 4086
Size: 910 654 1755 767
Fragments arranged by size:
1755 910 767 654
BstAPI GCAn_nnn'nTGC
Cuts at: 0 195 2802 2811 2907 3003 4086
Size: 195 2607 9 96 96 1083
Fragments arranged by size:
2607 1083 195 96 96 9
BstBI TT'CG_AA
Cuts at: 0 1349 4086
Size: 1349 2737
BstEII G'GTnAC_C
Cuts at: 0 3169 4086
Size: 3169 917
BstXI CCAn_nnnn'nTGG
Cuts at: 0 951 1437 4086
Size: 951 486 2649
Fragments arranged by size:
2649 951 486
BstYI r'GATC_y
Cuts at: 0 3341 4086
Size: 3341 745
Bsu36I CC'TnA_GG
Cuts at: 0 1382 4086
Size: 1382 2704
BtgI C'CryG_G
Cuts at: 0 581 1190 1322 2711 2980 3299 4019 4086
Size: 581 609 132 1389 269 319 720 67
Fragments arranged by size:
1389 720 609 581 319 269 132 67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 807 2389 4086
Size: 807 1582 1697
Fragments arranged by size:
1697 1582 807
BtsI GCAGTG_nn'
Cuts at: 0 736 1421 1987 2570 4086
Size: 736 685 566 583 1516
Fragments arranged by size:
1516 736 685 583 566
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 879 914 1416 1451 4086
Size: 879 35 502 35 2635
Fragments arranged by size:
2635 879 502 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1993 2565 4086
Size: 1993 572 1521
Fragments arranged by size:
1993 1521 572
DrdI GACnn_nn'nnGTC
Cuts at: 0 697 3243 4086
Size: 697 2546 843
Fragments arranged by size:
2546 843 697
EaeI y'GGCC_r
Cuts at: 0 827 1319 1397 2460 3388 3445 4086
Size: 827 492 78 1063 928 57 641
Fragments arranged by size:
1063 928 827 641 492 78 57
EagI C'GGCC_G
Cuts at: 0 3388 3445 4086
Size: 3388 57 641
Fragments arranged by size:
3388 641 57
EarI CTCTTCn'nnn_
Cuts at: 0 472 528 859 1030 1667 1768 1802 2741
Size: 472 56 331 171 637 101 34 939
Cuts at: 2741 4086
Size: 1345
Fragments arranged by size:
1345 939 637 472 331 171 101 56
34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3053 3670 4086
Size: 3053 617 416
Fragments arranged by size:
3053 617 416
EcoICRI GAG'CTC
Cuts at: 0 1539 2114 3941 4086
Size: 1539 575 1827 145
Fragments arranged by size:
1827 1539 575 145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 770 1240 1321 1525 1951 1996 2020
Size: 446 324 470 81 204 426 45 24
Cuts at: 2020 2100 2443 2561 2758 3357 3542 3742 3826
Size: 80 343 118 197 599 185 200 84
Cuts at: 3826 4086
Size: 260
Fragments arranged by size:
599 470 446 426 343 324 260 204
200 197 185 118 84 81 80 45
24
EcoO109I rG'GnC_Cy
Cuts at: 0 2244 3423 3518 3519 3576 3717 4086
Size: 2244 1179 95 1 57 141 369
Fragments arranged by size:
2244 1179 369 141 95 57 1
EcoRI G'AATT_C
Cuts at: 0 1935 4086
Size: 1935 2151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 354 486 4086
Size: 354 132 3600
Fragments arranged by size:
3600 354 132
HaeII r_GCGC'y
Cuts at: 0 339 1768 2007 3846 3870 4086
Size: 339 1429 239 1839 24 216
Fragments arranged by size:
1839 1429 339 239 216 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1462 1494 3145 3177 4045 4077 4086
Size: 1462 32 1651 32 868 32 9
Fragments arranged by size:
1651 1462 868 32 32 32 9
KasI G'GCGC_C
Cuts at: 0 3842 4086
Size: 3842 244
KpnI G_GTAC'C
Cuts at: 0 589 4086
Size: 589 3497
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 1304 1781 1964 2087 3048 3131 3652
Size: 176 1128 477 183 123 961 83 521
Cuts at: 3652 4086
Size: 434
Fragments arranged by size:
1128 961 521 477 434 183 176 123
83
MscI TGG'CCA
Cuts at: 0 1321 4086
Size: 1321 2765
MslI CAynn'nnrTG
Cuts at: 0 718 1195 2410 2773 2779 4036 4086
Size: 718 477 1215 363 6 1257 50
Fragments arranged by size:
1257 1215 718 477 363 50 6
MspA1I CmG'CkG
Cuts at: 0 960 1073 1527 2632 2787 2802 2907 3444
Size: 960 113 454 1105 155 15 105 537
Cuts at: 3444 3904 4086
Size: 460 182
Fragments arranged by size:
1105 960 537 460 454 182 155 113
105 15
NaeI GCC'GGC
Cuts at: 0 976 2070 4086
Size: 976 1094 2016
Fragments arranged by size:
2016 1094 976
NarI GG'CG_CC
Cuts at: 0 3843 4086
Size: 3843 243
NcoI C'CATG_G
Cuts at: 0 1190 1322 2711 4019 4086
Size: 1190 132 1389 1308 67
Fragments arranged by size:
1389 1308 1190 132 67
NgoMIV G'CCGG_C
Cuts at: 0 974 2068 4086
Size: 974 1094 2018
Fragments arranged by size:
2018 1094 974
PasI CC'CwG_GG
Cuts at: 0 19 3042 4086
Size: 19 3023 1044
Fragments arranged by size:
3023 1044 19
PflMI CCAn_nnn'nTGG
Cuts at: 0 1314 1530 4086
Size: 1314 216 2556
Fragments arranged by size:
2556 1314 216
PfoI T'CCnGG_A
Cuts at: 0 377 1337 1541 2420 4086
Size: 377 960 204 879 1666
Fragments arranged by size:
1666 960 879 377 204
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3583 3615 4086
Size: 3583 32 471
Fragments arranged by size:
3583 471 32
PpuMI rG'GwC_Cy
Cuts at: 0 3576 4086
Size: 3576 510
PshAI GACnn'nnGTC
Cuts at: 0 709 4086
Size: 709 3377
PspOMI G'GGCC_C
Cuts at: 0 3518 3821 4086
Size: 3518 303 265
Fragments arranged by size:
3518 303 265
PstI C_TGCA'G
Cuts at: 0 743 1072 2798 2807 2912 4086
Size: 743 329 1726 9 105 1174
Fragments arranged by size:
1726 1174 743 329 105 9
PvuII CAG'CTG
Cuts at: 0 2787 2802 2907 3904 4086
Size: 2787 15 105 997 182
Fragments arranged by size:
2787 997 182 105 15
SacI G_AGCT'C
Cuts at: 0 1541 2116 3943 4086
Size: 1541 575 1827 143
Fragments arranged by size:
1827 1541 575 143
SapI GCTCTTCn'nnn_
Cuts at: 0 472 1030 1768 2741 4086
Size: 472 558 738 973 1345
Fragments arranged by size:
1345 973 738 558 472
SfcI C'TryA_G
Cuts at: 0 739 1068 2794 2803 2908 4086
Size: 739 329 1726 9 105 1178
Fragments arranged by size:
1726 1178 739 329 105 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3820 4086
Size: 3820 266
SfoI GGC'GCC
Cuts at: 0 3844 4086
Size: 3844 242
SmaI CCC'GGG
Cuts at: 0 2193 4086
Size: 2193 1893
SmlI C'TyrA_G
Cuts at: 0 211 599 1060 1081 2042 4086
Size: 211 388 461 21 961 2044
Fragments arranged by size:
2044 961 461 388 211 21
SspI AAT'ATT
Cuts at: 0 4067 4086
Size: 4067 19
StuI AGG'CCT
Cuts at: 0 3477 4086
Size: 3477 609
StyI C'CwwG_G
Cuts at: 0 1190 1322 1430 1694 2711 4019 4086
Size: 1190 132 108 264 1017 1308 67
Fragments arranged by size:
1308 1190 1017 264 132 108 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 827 831 1037 2422 2485 3031 3366 4086
Size: 827 4 206 1385 63 546 335 720
Fragments arranged by size:
1385 827 720 546 335 206 63 4
TatI w'GTAC_w
Cuts at: 0 1292 4086
Size: 1292 2794
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2681 2713 3341 3373 4086
Size: 2681 32 628 32 713
Fragments arranged by size:
2681 713 628 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 436 4086
Size: 436 3650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1434 4086
Size: 1434 2652
XmaI C'CCGG_G
Cuts at: 0 2191 4086
Size: 2191 1895
XmnI GAAnn'nnTTC
Cuts at: 0 2089 4086
Size: 2089 1997
ZraI GAC'GTC
Cuts at: 0 1263 4086
Size: 1263 2823
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV PasI PflMI PfoI PpiI PpuMI
PshAI PspOMI PstI PvuII SacI SapI SfcI SfiI
SfoI SmaI SmlI SspI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AflIII AhdI AscI AseI AsiSI AvrII
BaeI BamHI BclI BfrBI BglII BmtI BsiWI BsmBI
BspEI BsrGI BssHII BstZ17I ClaI DraI EcoNI EcoRV
FseI FspI FspAI HincII HindIII HpaI MfeI MluI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SgfI SgrAI SnaBI
SpeI SphI SrfI SwaI XbaI XhoI