(Linear) (Six-base) MAPSORT of: KIEE9279.seq  Check: 1079  from: 1  to: 870



KIEE9279.orf

 With 182 enzymes: SgfI * 

                        March 29, 2013 15:36  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      24     870 
   Size:         24     846
 
AlwNI CAG_nnn'CTG

Cuts at:      0     726     870 
   Size:        726     144
 
AvaI C'yCGr_G

Cuts at:      0      69     870 
   Size:         69     801
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     525     870 
   Size:        525     345
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     527     870 
   Size:        527     343
 
BsaAI yAC'GTr

Cuts at:      0     708     870 
   Size:        708     162
 
BsaWI w'CCGG_w

Cuts at:      0     458     870 
   Size:        458     412
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      46      76     870 
   Size:         46      30     794

  Fragments arranged by size: 

                 794      46      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      58     112     870 
   Size:         58      54     758

  Fragments arranged by size: 

                 758      58      54
 
BseYI C'CCAG_C

Cuts at:      0     107     305     464     468     601     870 
   Size:        107     198     159       4     133     269

  Fragments arranged by size: 

                 269     198     159     133     107       4
 
BsiEI CG_ry'CG

Cuts at:      0     837     870 
   Size:        837      33
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      24     870 
   Size:         24     846
 
BsrFI r'CCGG_y

Cuts at:      0     832     870 
   Size:        832      38
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     786     870 
   Size:        786      84
 
DraIII CAC_nnn'GTG

Cuts at:      0     176     870 
   Size:        176     694
 
EaeI y'GGCC_r

Cuts at:      0     230     834     870 
   Size:        230     604      36

  Fragments arranged by size: 

                 604     230      36
 
EagI C'GGCC_G

Cuts at:      0     834     870 
   Size:        834      36
 
EarI CTCTTCn'nnn_

Cuts at:      0      16     130     870 
   Size:         16     114     740

  Fragments arranged by size: 

                 740     114      16
 
HaeII r_GCGC'y

Cuts at:      0     399     870 
   Size:        399     471
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     224     870 
   Size:        224     646
 
MscI TGG'CCA

Cuts at:      0     232     870 
   Size:        232     638
 
MslI CAynn'nnrTG

Cuts at:      0     157     178     541     870 
   Size:        157      21     363     329

  Fragments arranged by size: 

                 363     329     157      21
 
MspA1I CmG'CkG

Cuts at:      0     107     468     870 
   Size:        107     361     402

  Fragments arranged by size: 

                 402     361     107
 
NaeI GCC'GGC

Cuts at:      0     834     870 
   Size:        834      36
 
NgoMIV G'CCGG_C

Cuts at:      0     832     870 
   Size:        832      38
 
NspI r_CATG'y

Cuts at:      0     319     870 
   Size:        319     551
 
PasI CC'CwG_GG

Cuts at:      0     391     870 
   Size:        391     479
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      87     418     870 
   Size:         87     331     452

  Fragments arranged by size: 

                 452     331      87
 
PmlI CAC'GTG

Cuts at:      0     708     870 
   Size:        708     162
 
PstI C_TGCA'G

Cuts at:      0     191     870 
   Size:        191     679
 
PvuII CAG'CTG

Cuts at:      0     107     468     870 
   Size:        107     361     402

  Fragments arranged by size: 

                 402     361     107
 
SapI GCTCTTCn'nnn_

Cuts at:      0      16     870 
   Size:         16     854
 
SfcI C'TryA_G

Cuts at:      0     187     870 
   Size:        187     683
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     168     239     514     870 
   Size:        168      71     275     356

  Fragments arranged by size: 

                 356     275     168      71
 
TatI w'GTAC_w

Cuts at:      0     677     870 
   Size:        677     193
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      46      78     870 
   Size:         46      32     792

  Fragments arranged by size: 

                 792      46      32
 
XmnI GAAnn'nnTTC

Cuts at:      0      55     870 
   Size:         55     815
 

 Enzymes that do cut:

     AarI    AlwNI     AvaI     BbsI     BmrI    BsaAI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI    BspMI    BsrFI    BtgZI   DraIII     EaeI
     EagI     EarI    HaeII     MmeI     MscI     MslI   MspA1I     NaeI
   NgoMIV     NspI     PasI    PflMI     PmlI     PstI    PvuII     SapI
     SfcI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
     BtsI     ClaI    CspCI     DraI     DrdI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PciI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI