Restriction map for
pF1KE9260
(Linear) (Six-base) MAPSORT of: KIEE9260.seq Check: 3245 from: 1 to: 4746
KIEE9260.orf
With 182 enzymes: SgfI *
February 10, 2017 14:54 ..
AatII G_ACGT'C
Cuts at: 0 606 4592 4746
Size: 606 3986 154
Fragments arranged by size:
3986 606 154
AccI GT'mk_AC
Cuts at: 0 4087 4746
Size: 4087 659
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 1330 1487 2587 3383 3493 3855 4018
Size: 542 788 157 1100 796 110 362 163
Cuts at: 4018 4746
Size: 728
Fragments arranged by size:
1100 796 788 728 542 362 163 157
110
AflIII A'CryG_T
Cuts at: 0 3464 3679 4746
Size: 3464 215 1067
Fragments arranged by size:
3464 1067 215
AhdI GACnn_n'nnGTC
Cuts at: 0 4429 4588 4746
Size: 4429 159 158
Fragments arranged by size:
4429 159 158
AlwNI CAG_nnn'CTG
Cuts at: 0 520 630 4569 4746
Size: 520 110 3939 177
Fragments arranged by size:
3939 520 177 110
ApaI G_GGCC'C
Cuts at: 0 1879 4653 4746
Size: 1879 2774 93
Fragments arranged by size:
2774 1879 93
ApoI r'AATT_y
Cuts at: 0 819 1356 2123 2417 2621 3031 3089 3808
Size: 819 537 767 294 204 410 58 719
Cuts at: 3808 3860 3943 4746
Size: 52 83 803
Fragments arranged by size:
819 803 767 719 537 410 294 204
83 58 52
AvaI C'yCGr_G
Cuts at: 0 1598 3260 4746
Size: 1598 1662 1486
Fragments arranged by size:
1662 1598 1486
BamHI G'GATC_C
Cuts at: 0 2430 4746
Size: 2430 2316
BanI G'GyrC_C
Cuts at: 0 65 222 4389 4679 4746
Size: 65 157 4167 290 67
Fragments arranged by size:
4167 290 157 67 65
BanII G_rGCy'C
Cuts at: 0 212 1813 1879 3617 4226 4653 4746
Size: 212 1601 66 1738 609 427 93
Fragments arranged by size:
1738 1601 609 427 212 93 66
BbeI G_GCGC'C
Cuts at: 0 4683 4746
Size: 4683 63
BbsI GAAGACnn'nnnn_
Cuts at: 0 880 1626 1769 3371 3483 4587 4746
Size: 880 746 143 1602 112 1104 159
Fragments arranged by size:
1602 1104 880 746 159 143 112
BbvCI CC'TCA_GC
Cuts at: 0 4028 4746
Size: 4028 718
BciVI GTATCCnnnnn_n'
Cuts at: 0 701 4746
Size: 701 4045
BglI GCCn_nnn'nGGC
Cuts at: 0 2822 4380 4746
Size: 2822 1558 366
Fragments arranged by size:
2822 1558 366
BglII A'GATC_T
Cuts at: 0 684 756 2068 4746
Size: 684 72 1312 2678
Fragments arranged by size:
2678 1312 684 72
BlpI GC'TnA_GC
Cuts at: 0 4225 4746
Size: 4225 521
Bme1580I G_kGCm'C
Cuts at: 0 68 225 514 1394 1879 4653 4746
Size: 68 157 289 880 485 2774 93
Fragments arranged by size:
2774 880 485 289 157 93 68
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 813 1827 4746
Size: 813 1014 2919
Fragments arranged by size:
2919 1014 813
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 577 1861 2308 4310 4361 4746
Size: 518 59 1284 447 2002 51 385
Fragments arranged by size:
2002 1284 518 447 385 59 51
Bpu10I CC'TnA_GC
Cuts at: 0 4028 4746
Size: 4028 718
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 785 1837 1900 4279 4746
Size: 673 112 1052 63 2379 467
Fragments arranged by size:
2379 1052 673 467 112 63
BsaI GGTCTCn'nnnn_
Cuts at: 0 2276 4746
Size: 2276 2470
BsaBI GATnn'nnATC
Cuts at: 0 286 755 4746
Size: 286 469 3991
Fragments arranged by size:
3991 469 286
BsaHI Gr'CG_yC
Cuts at: 0 603 4589 4680 4746
Size: 603 3986 91 66
Fragments arranged by size:
3986 603 91 66
BsaWI w'CCGG_w
Cuts at: 0 1781 4746
Size: 1781 2965
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2873 2903 3709 3739 4746
Size: 2873 30 806 30 1007
Fragments arranged by size:
2873 1007 806 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 120 751 1819 4746
Size: 120 631 1068 2927
Fragments arranged by size:
2927 1068 631 120
BseYI C'CCAG_C
Cuts at: 0 1872 4481 4746
Size: 1872 2609 265
Fragments arranged by size:
2609 1872 265
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1073 4746
Size: 1073 3673
BsiHKAI G_wGCw'C
Cuts at: 0 212 4226 4746
Size: 212 4014 520
Fragments arranged by size:
4014 520 212
BsmI GAATG_Cn'
Cuts at: 0 1791 4746
Size: 1791 2955
BsmBI CGTCTCn'nnnn_
Cuts at: 0 610 1463 4746
Size: 610 853 3283
Fragments arranged by size:
3283 853 610
Bsp1286I G_dGCh'C
Cuts at: 0 68 212 225 514 1394 1813 1879 3617
Size: 68 144 13 289 880 419 66 1738
Cuts at: 3617 4226 4653 4746
Size: 609 427 93
Fragments arranged by size:
1738 880 609 427 419 289 144 93
68 66 13
BspEI T'CCGG_A
Cuts at: 0 1781 4746
Size: 1781 2965
BspHI T'CATG_A
Cuts at: 0 867 2186 2366 4746
Size: 867 1319 180 2380
Fragments arranged by size:
2380 1319 867 180
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2910 3676 4746
Size: 2910 766 1070
Fragments arranged by size:
2910 1070 766
BsrDI GCAATG_nn'
Cuts at: 0 695 746 1734 4746
Size: 695 51 988 3012
Fragments arranged by size:
3012 988 695 51
BsrFI r'CCGG_y
Cuts at: 0 2664 4746
Size: 2664 2082
BsrGI T'GTAC_A
Cuts at: 0 2206 4746
Size: 2206 2540
BssSI C'ACGA_G
Cuts at: 0 3521 4746
Size: 3521 1225
BstAPI GCAn_nnn'nTGC
Cuts at: 0 772 1437 4746
Size: 772 665 3309
Fragments arranged by size:
3309 772 665
BstBI TT'CG_AA
Cuts at: 0 435 891 2127 4746
Size: 435 456 1236 2619
Fragments arranged by size:
2619 1236 456 435
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2115 4056 4746
Size: 2115 1941 690
Fragments arranged by size:
2115 1941 690
BstYI r'GATC_y
Cuts at: 0 132 526 551 684 756 1295 2068 2430
Size: 132 394 25 133 72 539 773 362
Cuts at: 2430 3923 4746
Size: 1493 823
Fragments arranged by size:
1493 823 773 539 394 362 133 132
72 25
BtgI C'CryG_G
Cuts at: 0 2108 3504 4661 4746
Size: 2108 1396 1157 85
Fragments arranged by size:
2108 1396 1157 85
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2815 4746
Size: 2815 1931
BtsI GCAGTG_nn'
Cuts at: 0 45 1879 4116 4176 4746
Size: 45 1834 2237 60 570
Fragments arranged by size:
2237 1834 570 60 45
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4131 4166 4746
Size: 4131 35 580
Fragments arranged by size:
4131 580 35
DraI TTT'AAA
Cuts at: 0 2859 4746
Size: 2859 1887
DraIII CAC_nnn'GTG
Cuts at: 0 4336 4746
Size: 4336 410
DrdI GACnn_nn'nnGTC
Cuts at: 0 932 2308 4746
Size: 932 1376 2438
Fragments arranged by size:
2438 1376 932
EaeI y'GGCC_r
Cuts at: 0 743 2141 4046 4746
Size: 743 1398 1905 700
Fragments arranged by size:
1905 1398 743 700
EarI CTCTTCn'nnn_
Cuts at: 0 12 217 305 959 1094 1506 1742 1924
Size: 12 205 88 654 135 412 236 182
Cuts at: 1924 3817 3974 4746
Size: 1893 157 772
Fragments arranged by size:
1893 772 654 412 236 205 182 157
135 88 12
EcoICRI GAG'CTC
Cuts at: 0 210 4224 4746
Size: 210 4014 522
Fragments arranged by size:
4014 522 210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 542 577 1330 1487 1861 2308 2587
Size: 518 24 35 753 157 374 447 279
Cuts at: 2587 3383 3493 3855 4018 4310 4361 4746
Size: 796 110 362 163 292 51 385
Fragments arranged by size:
796 753 518 447 385 374 362 292
279 163 157 110 51 35 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4316 4746
Size: 4316 430
EcoO109I rG'GnC_Cy
Cuts at: 0 934 4372 4649 4746
Size: 934 3438 277 97
Fragments arranged by size:
3438 934 277 97
EcoRV GAT'ATC
Cuts at: 0 1476 4746
Size: 1476 3270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 973 1145 2263 2284 3144 4746
Size: 973 172 1118 21 860 1602
Fragments arranged by size:
1602 1118 973 860 172 21
HaeII r_GCGC'y
Cuts at: 0 4683 4746
Size: 4683 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1093 1125 1460 1492 2149 2181 2209 2241
Size: 1093 32 335 32 657 32 28 32
Cuts at: 2241 2287 2319 2873 2905 3709 3741 4415 4447
Size: 46 32 554 32 804 32 674 32
Cuts at: 4447 4574 4606 4746
Size: 127 32 140
Fragments arranged by size:
1093 804 674 657 554 335 140 127
46 32 32 32 32 32 32 32
32 32 28
HincII GTy'rAC
Cuts at: 0 80 4746
Size: 80 4666
KasI G'GCGC_C
Cuts at: 0 4679 4746
Size: 4679 67
MfeI C'AATT_G
Cuts at: 0 3881 4746
Size: 3881 865
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 315 932 1168 2859 4565 4746
Size: 148 167 617 236 1691 1706 181
Fragments arranged by size:
1706 1691 617 236 181 167 148
MscI TGG'CCA
Cuts at: 0 745 2143 4048 4746
Size: 745 1398 1905 698
Fragments arranged by size:
1905 1398 745 698
MslI CAynn'nnrTG
Cuts at: 0 1799 2113 2378 2699 3458 3622 4660 4746
Size: 1799 314 265 321 759 164 1038 86
Fragments arranged by size:
1799 1038 759 321 314 265 164 86
MspA1I CmG'CkG
Cuts at: 0 47 2962 4420 4485 4746
Size: 47 2915 1458 65 261
Fragments arranged by size:
2915 1458 261 65 47
NarI GG'CG_CC
Cuts at: 0 4680 4746
Size: 4680 66
NcoI C'CATG_G
Cuts at: 0 2108 3504 4661 4746
Size: 2108 1396 1157 85
Fragments arranged by size:
2108 1396 1157 85
NdeI CA'TA_TG
Cuts at: 0 2528 4347 4746
Size: 2528 1819 399
Fragments arranged by size:
2528 1819 399
NspI r_CATG'y
Cuts at: 0 2871 3468 3683 4746
Size: 2871 597 215 1063
Fragments arranged by size:
2871 1063 597 215
PciI A'CATG_T
Cuts at: 0 3464 3679 4746
Size: 3464 215 1067
Fragments arranged by size:
3464 1067 215
PflMI CCAn_nnn'nTGG
Cuts at: 0 3510 4305 4746
Size: 3510 795 441
Fragments arranged by size:
3510 795 441
PfoI T'CCnGG_A
Cuts at: 0 554 1838 4700 4746
Size: 554 1284 2862 46
Fragments arranged by size:
2862 1284 554 46
PpuMI rG'GwC_Cy
Cuts at: 0 934 4746
Size: 934 3812
PsiI TTA'TAA
Cuts at: 0 380 785 1676 1823 4746
Size: 380 405 891 147 2923
Fragments arranged by size:
2923 891 405 380 147
PspOMI G'GGCC_C
Cuts at: 0 1875 4649 4746
Size: 1875 2774 97
Fragments arranged by size:
2774 1875 97
PstI C_TGCA'G
Cuts at: 0 921 2814 2907 3733 4746
Size: 921 1893 93 826 1013
Fragments arranged by size:
1893 1013 921 826 93
PvuII CAG'CTG
Cuts at: 0 2962 4420 4746
Size: 2962 1458 326
Fragments arranged by size:
2962 1458 326
SacI G_AGCT'C
Cuts at: 0 212 4226 4746
Size: 212 4014 520
Fragments arranged by size:
4014 520 212
SapI GCTCTTCn'nnn_
Cuts at: 0 12 217 4746
Size: 12 205 4529
Fragments arranged by size:
4529 205 12
SbfI CC_TGCA'GG
Cuts at: 0 3733 4746
Size: 3733 1013
SexAI A'CCwGG_T
Cuts at: 0 3426 4332 4746
Size: 3426 906 414
Fragments arranged by size:
3426 906 414
SfcI C'TryA_G
Cuts at: 0 128 680 917 2397 2810 2850 2903 3729
Size: 128 552 237 1480 413 40 53 826
Cuts at: 3729 4746
Size: 1017
Fragments arranged by size:
1480 1017 826 552 413 237 128 53
40
SfoI GGC'GCC
Cuts at: 0 4681 4746
Size: 4681 65
SmlI C'TyrA_G
Cuts at: 0 688 800 1598 1852 1915 4258 4746
Size: 688 112 798 254 63 2343 488
Fragments arranged by size:
2343 798 688 488 254 112 63
SspI AAT'ATT
Cuts at: 0 836 4746
Size: 836 3910
StuI AGG'CCT
Cuts at: 0 2816 4504 4746
Size: 2816 1688 242
Fragments arranged by size:
2816 1688 242
StyI C'CwwG_G
Cuts at: 0 957 1197 2108 3504 4299 4661 4746
Size: 957 240 911 1396 795 362 85
Fragments arranged by size:
1396 957 911 795 362 240 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3608 4217 4467 4746
Size: 3608 609 250 279
Fragments arranged by size:
3608 609 279 250
TatI w'GTAC_w
Cuts at: 0 1008 2206 4746
Size: 1008 1198 2540
Fragments arranged by size:
2540 1198 1008
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4343 4375 4746
Size: 4343 32 371
Fragments arranged by size:
4343 371 32
Tth111I GACn'n_nGTC
Cuts at: 0 2182 3487 4746
Size: 2182 1305 1259
Fragments arranged by size:
2182 1305 1259
XbaI T'CTAG_A
Cuts at: 0 2065 3036 4746
Size: 2065 971 1710
Fragments arranged by size:
2065 1710 971
XhoI C'TCGA_G
Cuts at: 0 1598 4746
Size: 1598 3148
XmnI GAAnn'nnTTC
Cuts at: 0 620 2123 3644 3808 4746
Size: 620 1503 1521 164 938
Fragments arranged by size:
1521 1503 938 620 164
ZraI GAC'GTC
Cuts at: 0 604 4590 4746
Size: 604 3986 156
Fragments arranged by size:
3986 604 156
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BglI BglII BlpI Bme1580I BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I
HincII KasI MfeI MmeI MscI MslI MspA1I NarI
NcoI NdeI NspI PciI PflMI PfoI PpuMI PsiI
PspOMI PstI PvuII SacI SapI SbfI SexAI SfcI
SfoI SmlI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BcgI
BclI BfrBI BmgBI BmrI BmtI BsaAI BsiEI BsiWI
BsrBI BssHII BstEII BstZ17I Bsu36I ClaI EagI EciI
EcoRI FseI FspI FspAI HindIII HpaI KpnI MluI
NaeI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII
SacII SalI SanDI ScaI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SwaI XcmI XmaI