Restriction map for
pF1KE8000
(Linear) (Six-base) MAPSORT of: KIEE8000.seq Check: 8721 from: 1 to: 1302
KIEE8000.orf
With 182 enzymes: SgfI *
February 29, 2012 11:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 925 1302
Size: 925 377
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 774 847 1009 1138 1192 1302
Size: 774 73 162 129 54 110
Fragments arranged by size:
774 162 129 110 73 54
AflIII A'CryG_T
Cuts at: 0 169 734 746 1302
Size: 169 565 12 556
Fragments arranged by size:
565 556 169 12
AhdI GACnn_n'nnGTC
Cuts at: 0 60 1302
Size: 60 1242
AlwNI CAG_nnn'CTG
Cuts at: 0 64 411 426 542 665 1230 1302
Size: 64 347 15 116 123 565 72
Fragments arranged by size:
565 347 123 116 72 64 15
ApoI r'AATT_y
Cuts at: 0 510 973 1302
Size: 510 463 329
Fragments arranged by size:
510 463 329
BanI G'GyrC_C
Cuts at: 0 227 244 1302
Size: 227 17 1058
Fragments arranged by size:
1058 227 17
BanII G_rGCy'C
Cuts at: 0 965 1199 1302
Size: 965 234 103
Fragments arranged by size:
965 234 103
BbeI G_GCGC'C
Cuts at: 0 248 1302
Size: 248 1054
BbsI GAAGACnn'nnnn_
Cuts at: 0 271 932 1302
Size: 271 661 370
Fragments arranged by size:
661 370 271
BbvCI CC'TCA_GC
Cuts at: 0 223 1302
Size: 223 1079
BclI T'GATC_A
Cuts at: 0 332 371 449 1302
Size: 332 39 78 853
Fragments arranged by size:
853 332 78 39
BglII A'GATC_T
Cuts at: 0 612 704 815 1302
Size: 612 92 111 487
Fragments arranged by size:
612 487 111 92
BlpI GC'TnA_GC
Cuts at: 0 532 1214 1302
Size: 532 682 88
Fragments arranged by size:
682 532 88
Bme1580I G_kGCm'C
Cuts at: 0 232 1302
Size: 232 1070
BmrI ACTGGGnnnn_n'
Cuts at: 0 74 421 1302
Size: 74 347 881
Fragments arranged by size:
881 347 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 726 1302
Size: 726 576
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 979 1302
Size: 328 651 323
Fragments arranged by size:
651 328 323
Bpu10I CC'TnA_GC
Cuts at: 0 223 1302
Size: 223 1079
BsaI GGTCTCn'nnnn_
Cuts at: 0 3 636 1302
Size: 3 633 666
Fragments arranged by size:
666 633 3
BsaBI GATnn'nnATC
Cuts at: 0 448 1302
Size: 448 854
BsaHI Gr'CG_yC
Cuts at: 0 245 1302
Size: 245 1057
BsaWI w'CCGG_w
Cuts at: 0 236 605 1302
Size: 236 369 697
Fragments arranged by size:
697 369 236
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 802 832 1302
Size: 802 30 470
Fragments arranged by size:
802 470 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 448 1024 1027 1302
Size: 370 78 576 3 275
Fragments arranged by size:
576 370 275 78 3
BseYI C'CCAG_C
Cuts at: 0 681 1145 1223 1302
Size: 681 464 78 79
Fragments arranged by size:
681 464 79 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 1302
Size: 439 863
BsiHKAI G_wGCw'C
Cuts at: 0 622 1199 1302
Size: 622 577 103
Fragments arranged by size:
622 577 103
BsmI GAATG_Cn'
Cuts at: 0 1138 1302
Size: 1138 164
Bsp1286I G_dGCh'C
Cuts at: 0 232 622 965 1199 1302
Size: 232 390 343 234 103
Fragments arranged by size:
390 343 234 232 103
BspEI T'CCGG_A
Cuts at: 0 236 605 1302
Size: 236 369 697
Fragments arranged by size:
697 369 236
BspHI T'CATG_A
Cuts at: 0 164 1302
Size: 164 1138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 925 1302
Size: 925 377
BsrBI CCG'CTC
Cuts at: 0 314 1302
Size: 314 988
BsrFI r'CCGG_y
Cuts at: 0 905 1302
Size: 905 397
BsrGI T'GTAC_A
Cuts at: 0 42 1302
Size: 42 1260
BstAPI GCAn_nnn'nTGC
Cuts at: 0 542 1302
Size: 542 760
BstYI r'GATC_y
Cuts at: 0 612 704 815 1302
Size: 612 92 111 487
Fragments arranged by size:
612 487 111 92
BtgI C'CryG_G
Cuts at: 0 350 1302
Size: 350 952
BtsI GCAGTG_nn'
Cuts at: 0 543 666 929 1302
Size: 543 123 263 373
Fragments arranged by size:
543 373 263 123
DraIII CAC_nnn'GTG
Cuts at: 0 1080 1302
Size: 1080 222
EaeI y'GGCC_r
Cuts at: 0 903 1302
Size: 903 399
EarI CTCTTCn'nnn_
Cuts at: 0 1048 1302
Size: 1048 254
EcoICRI GAG'CTC
Cuts at: 0 1197 1302
Size: 1197 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 774 847 979 1009 1138 1192 1302
Size: 328 446 73 132 30 129 54 110
Fragments arranged by size:
446 328 132 129 110 73 54 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 789 826 1273 1302
Size: 789 37 447 29
Fragments arranged by size:
789 447 37 29
EcoO109I rG'GnC_Cy
Cuts at: 0 109 567 1302
Size: 109 458 735
Fragments arranged by size:
735 458 109
FspI TGC'GCA
Cuts at: 0 298 1237 1302
Size: 298 939 65
Fragments arranged by size:
939 298 65
HaeII r_GCGC'y
Cuts at: 0 248 1302
Size: 248 1054
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 46 78 387 419 453 485 604 636
Size: 46 32 309 32 34 32 119 32
Cuts at: 636 802 834 1302
Size: 166 32 468
Fragments arranged by size:
468 309 166 119 46 34 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 87 740 853 1302
Size: 87 653 113 449
Fragments arranged by size:
653 449 113 87
KasI G'GCGC_C
Cuts at: 0 244 1302
Size: 244 1058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 365 1302
Size: 84 281 937
Fragments arranged by size:
937 281 84
MslI CAynn'nnrTG
Cuts at: 0 158 1183 1302
Size: 158 1025 119
Fragments arranged by size:
1025 158 119
MspA1I CmG'CkG
Cuts at: 0 441 642 1002 1092 1227 1302
Size: 441 201 360 90 135 75
Fragments arranged by size:
441 360 201 135 90 75
NaeI GCC'GGC
Cuts at: 0 907 1302
Size: 907 395
NarI GG'CG_CC
Cuts at: 0 245 1302
Size: 245 1057
NgoMIV G'CCGG_C
Cuts at: 0 905 1302
Size: 905 397
NspI r_CATG'y
Cuts at: 0 173 738 750 987 1302
Size: 173 565 12 237 315
Fragments arranged by size:
565 315 237 173 12
PciI A'CATG_T
Cuts at: 0 169 734 746 1302
Size: 169 565 12 556
Fragments arranged by size:
565 556 169 12
PfoI T'CCnGG_A
Cuts at: 0 69 1241 1302
Size: 69 1172 61
Fragments arranged by size:
1172 69 61
PpuMI rG'GwC_Cy
Cuts at: 0 109 1302
Size: 109 1193
PstI C_TGCA'G
Cuts at: 0 566 641 936 1001 1073 1097 1302
Size: 566 75 295 65 72 24 205
Fragments arranged by size:
566 295 205 75 72 65 24
PvuII CAG'CTG
Cuts at: 0 642 1002 1092 1302
Size: 642 360 90 210
Fragments arranged by size:
642 360 210 90
SacI G_AGCT'C
Cuts at: 0 1199 1302
Size: 1199 103
SfcI C'TryA_G
Cuts at: 0 562 637 932 997 1069 1093 1302
Size: 562 75 295 65 72 24 209
Fragments arranged by size:
562 295 209 75 72 65 24
SfoI GGC'GCC
Cuts at: 0 246 1302
Size: 246 1056
StuI AGG'CCT
Cuts at: 0 286 1302
Size: 286 1016
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1237 1302
Size: 1237 65
TatI w'GTAC_w
Cuts at: 0 42 1302
Size: 42 1260
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 372 404 1302
Size: 372 32 898
Fragments arranged by size:
898 372 32
Enzymes that do cut:
AarI AcuI AflIII AhdI AlwNI ApoI BanI BanII
BbeI BbsI BbvCI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BsrGI BstAPI BstYI BtgI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PpuMI PstI PvuII
SacI SfcI SfoI StuI TaqII TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BcgI BciVI BfrBI BglI BmgBI
BmtI BpuEI BsaAI BsiEI BsiWI BsmBI BsrDI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI KpnI MfeI MluI MscI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StyI SwaI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI