Restriction map for
pF1KE6728
(Linear) (Six-base) MAPSORT of: KIEE6728.seq Check: 1490 from: 1 to: 2925
KIEE6728.orf
With 182 enzymes: SgfI *
October 20, 2017 17:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 1151 1247 2791 2925
Size: 16 1135 96 1544 134
Fragments arranged by size:
1544 1135 134 96 16
AflII C'TTAA_G
Cuts at: 0 1533 2925
Size: 1533 1392
AflIII A'CryG_T
Cuts at: 0 225 1977 2925
Size: 225 1752 948
Fragments arranged by size:
1752 948 225
AleI CACnn'nnGTG
Cuts at: 0 1318 2925
Size: 1318 1607
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1663 1695 2840 2872 2925
Size: 1663 32 1145 32 53
Fragments arranged by size:
1663 1145 53 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 747 1701 2925
Size: 747 954 1224
Fragments arranged by size:
1224 954 747
ApoI r'AATT_y
Cuts at: 0 527 889 1011 1519 1639 2158 2446 2596
Size: 527 362 122 508 120 519 288 150
Cuts at: 2596 2925
Size: 329
Fragments arranged by size:
527 519 508 362 329 288 150 122
120
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 53 86 2925
Size: 53 33 2839
Fragments arranged by size:
2839 53 33
BanII G_rGCy'C
Cuts at: 0 281 1391 2925
Size: 281 1110 1534
Fragments arranged by size:
1534 1110 281
BbsI GAAGACnn'nnnn_
Cuts at: 0 1163 2925
Size: 1163 1762
BbvCI CC'TCA_GC
Cuts at: 0 83 2925
Size: 83 2842
BciVI GTATCCnnnnn_n'
Cuts at: 0 54 2360 2874 2925
Size: 54 2306 514 51
Fragments arranged by size:
2306 514 54 51
BclI T'GATC_A
Cuts at: 0 1410 2925
Size: 1410 1515
BfrBI ATG'CAT
Cuts at: 0 1734 2925
Size: 1734 1191
BmgBI CAC'GTC
Cuts at: 0 226 2925
Size: 226 2699
BmrI ACTGGGnnnn_n'
Cuts at: 0 1933 2925
Size: 1933 992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 2925
Size: 240 2685
Bpu10I CC'TnA_GC
Cuts at: 0 83 2925
Size: 83 2842
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2230 2719 2806 2925
Size: 2230 489 87 119
Fragments arranged by size:
2230 489 119 87
BsaAI yAC'GTr
Cuts at: 0 2369 2925
Size: 2369 556
BsaBI GATnn'nnATC
Cuts at: 0 848 1409 2925
Size: 848 561 1516
Fragments arranged by size:
1516 848 561
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1257 1287 2925
Size: 1257 30 1638
Fragments arranged by size:
1638 1257 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 265 268 857 1205 2925
Size: 100 165 3 589 348 1720
Fragments arranged by size:
1720 589 348 165 100 3
BseYI C'CCAG_C
Cuts at: 0 2519 2897 2925
Size: 2519 378 28
Fragments arranged by size:
2519 378 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 2925
Size: 293 2632
BsiHKAI G_wGCw'C
Cuts at: 0 335 1391 2246 2925
Size: 335 1056 855 679
Fragments arranged by size:
1056 855 679 335
BsmI GAATG_Cn'
Cuts at: 0 757 1063 1338 2925
Size: 757 306 275 1587
Fragments arranged by size:
1587 757 306 275
BsmBI CGTCTCn'nnnn_
Cuts at: 0 216 2925
Size: 216 2709
Bsp1286I G_dGCh'C
Cuts at: 0 281 335 1391 2246 2925
Size: 281 54 1056 855 679
Fragments arranged by size:
1056 855 679 281 54
BspHI T'CATG_A
Cuts at: 0 2642 2925
Size: 2642 283
BsrDI GCAATG_nn'
Cuts at: 0 335 603 1471 2925
Size: 335 268 868 1454
Fragments arranged by size:
1454 868 335 268
BstAPI GCAn_nnn'nTGC
Cuts at: 0 238 346 1740 2838 2925
Size: 238 108 1394 1098 87
Fragments arranged by size:
1394 1098 238 108 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 471 1320 2925
Size: 471 849 1605
Fragments arranged by size:
1605 849 471
BstYI r'GATC_y
Cuts at: 0 3 2652 2925
Size: 3 2649 273
Fragments arranged by size:
2649 273 3
BtsI GCAGTG_nn'
Cuts at: 0 106 335 631 2794 2925
Size: 106 229 296 2163 131
Fragments arranged by size:
2163 296 229 131 106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2355 2390 2925
Size: 2355 35 535
Fragments arranged by size:
2355 535 35
DraI TTT'AAA
Cuts at: 0 198 1422 2210 2925
Size: 198 1224 788 715
Fragments arranged by size:
1224 788 715 198
EarI CTCTTCn'nnn_
Cuts at: 0 2567 2925
Size: 2567 358
EcoICRI GAG'CTC
Cuts at: 0 1389 2925
Size: 1389 1536
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 240 1151 1247 2791 2925
Size: 16 224 911 96 1544 134
Fragments arranged by size:
1544 911 224 134 96 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2660 2925
Size: 2660 265
EcoO109I rG'GnC_Cy
Cuts at: 0 1309 2775 2925
Size: 1309 1466 150
Fragments arranged by size:
1466 1309 150
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1548 2925
Size: 1548 1377
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 303 335 935 967 1257 1289 2637 2669
Size: 303 32 600 32 290 32 1348 32
Cuts at: 2669 2679 2711 2925
Size: 10 32 214
Fragments arranged by size:
1348 600 303 290 214 32 32 32
32 32 10
HincII GTy'rAC
Cuts at: 0 41 662 1622 1773 2925
Size: 41 621 960 151 1152
Fragments arranged by size:
1152 960 621 151 41
HpaI GTT'AAC
Cuts at: 0 41 662 1622 2925
Size: 41 621 960 1303
Fragments arranged by size:
1303 960 621 41
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1350 2925
Size: 1350 1575
MslI CAynn'nnrTG
Cuts at: 0 1318 1729 2925
Size: 1318 411 1196
Fragments arranged by size:
1318 1196 411
MspA1I CmG'CkG
Cuts at: 0 82 2925
Size: 82 2843
NdeI CA'TA_TG
Cuts at: 0 1791 2925
Size: 1791 1134
NsiI A_TGCA'T
Cuts at: 0 1736 2925
Size: 1736 1189
NspI r_CATG'y
Cuts at: 0 1738 1747 1779 1981 2925
Size: 1738 9 32 202 944
Fragments arranged by size:
1738 944 202 32 9
PciI A'CATG_T
Cuts at: 0 1977 2925
Size: 1977 948
PflMI CCAn_nnn'nTGG
Cuts at: 0 159 371 2472 2925
Size: 159 212 2101 453
Fragments arranged by size:
2101 453 212 159
PfoI T'CCnGG_A
Cuts at: 0 413 2925
Size: 413 2512
PpuMI rG'GwC_Cy
Cuts at: 0 1309 2775 2925
Size: 1309 1466 150
Fragments arranged by size:
1466 1309 150
PsiI TTA'TAA
Cuts at: 0 533 998 2611 2672 2925
Size: 533 465 1613 61 253
Fragments arranged by size:
1613 533 465 253 61
PstI C_TGCA'G
Cuts at: 0 1706 2801 2925
Size: 1706 1095 124
Fragments arranged by size:
1706 1095 124
PvuII CAG'CTG
Cuts at: 0 82 2925
Size: 82 2843
SacI G_AGCT'C
Cuts at: 0 1391 2925
Size: 1391 1534
SfcI C'TryA_G
Cuts at: 0 1702 2222 2354 2797 2902 2925
Size: 1702 520 132 443 105 23
Fragments arranged by size:
1702 520 443 132 105 23
SmlI C'TyrA_G
Cuts at: 0 1533 2245 2698 2821 2925
Size: 1533 712 453 123 104
Fragments arranged by size:
1533 712 453 123 104
SpeI A'CTAG_T
Cuts at: 0 2407 2925
Size: 2407 518
SspI AAT'ATT
Cuts at: 0 141 725 1813 2925
Size: 141 584 1088 1112
Fragments arranged by size:
1112 1088 584 141
StuI AGG'CCT
Cuts at: 0 2630 2925
Size: 2630 295
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1489 2748 2911 2925
Size: 1489 1259 163 14
Fragments arranged by size:
1489 1259 163 14
TatI w'GTAC_w
Cuts at: 0 186 1278 1954 2925
Size: 186 1092 676 971
Fragments arranged by size:
1092 971 676 186
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1821 1853 2925
Size: 1821 32 1072
Fragments arranged by size:
1821 1072 32
XbaI T'CTAG_A
Cuts at: 0 1284 2925
Size: 1284 1641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 156 2925
Size: 156 2769
XmnI GAAnn'nnTTC
Cuts at: 0 142 383 2538 2925
Size: 142 241 2155 387
Fragments arranged by size:
2155 387 241 142
Enzymes that do cut:
AcuI AflII AflIII AleI AloI AlwNI ApoI BaeI
BanII BbsI BbvCI BciVI BclI BfrBI BmgBI BmrI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrDI BstAPI
BstXI BstYI BtsI CspCI DraI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI Hin4I HincII HpaI MmeI MslI
MspA1I NdeI NsiI NspI PciI PflMI PfoI PpuMI
PsiI PstI PvuII SacI SfcI SmlI SpeI SspI
StuI TaqII TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BamHI BanI BbeI BcgI BglI BglII BlpI Bme1580I
BmtI BplI BsaI BsaHI BsaWI BsiEI BsiWI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI DraIII DrdI EaeI
EagI EciI EcoRI EcoRV FseI FspI FspAI HaeII
HindIII KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NgoMIV NheI NotI NruI PacI PasI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SphI SrfI StyI
SwaI Tth111I XhoI XmaI ZraI