Restriction map for
pF1KE6704
(Linear) (Six-base) MAPSORT of: KIEE6704.seq Check: 2209 from: 1 to: 3183
KIEE6704.orf
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AatII G_ACGT'C
Cuts at: 0 1282 1472 3183
Size: 1282 190 1711
Fragments arranged by size:
1711 1282 190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 789 1824 1981 2215 2266 2608 3183
Size: 94 695 1035 157 234 51 342 575
Fragments arranged by size:
1035 695 575 342 234 157 94 51
AfeI AGC'GCT
Cuts at: 0 1966 3146 3183
Size: 1966 1180 37
Fragments arranged by size:
1966 1180 37
AflIII A'CryG_T
Cuts at: 0 1643 3183
Size: 1643 1540
AgeI A'CCGG_T
Cuts at: 0 464 3183
Size: 464 2719
AleI CACnn'nnGTG
Cuts at: 0 1297 3183
Size: 1297 1886
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 688 2380 3183
Size: 688 1692 803
Fragments arranged by size:
1692 803 688
AlwNI CAG_nnn'CTG
Cuts at: 0 528 812 1251 1887 1986 2238 2550 2655
Size: 528 284 439 636 99 252 312 105
Cuts at: 2655 2751 2922 3183
Size: 96 171 261
Fragments arranged by size:
636 528 439 312 284 261 252 171
105 99 96
ApaI G_GGCC'C
Cuts at: 0 1347 3183
Size: 1347 1836
ApaLI G'TGCA_C
Cuts at: 0 2127 3183
Size: 2127 1056
ApoI r'AATT_y
Cuts at: 0 406 1555 2310 3183
Size: 406 1149 755 873
Fragments arranged by size:
1149 873 755 406
AvaI C'yCGr_G
Cuts at: 0 58 619 856 2695 3183
Size: 58 561 237 1839 488
Fragments arranged by size:
1839 561 488 237 58
AvrII C'CTAG_G
Cuts at: 0 438 3027 3183
Size: 438 2589 156
Fragments arranged by size:
2589 438 156
BamHI G'GATC_C
Cuts at: 0 1781 3183
Size: 1781 1402
BanI G'GyrC_C
Cuts at: 0 929 1999 2252 2470 2555 3183
Size: 929 1070 253 218 85 628
Fragments arranged by size:
1070 929 628 253 218 85
BanII G_rGCy'C
Cuts at: 0 824 845 896 1347 1406 1828 2779 3183
Size: 824 21 51 451 59 422 951 404
Fragments arranged by size:
951 824 451 422 404 59 51 21
BbeI G_GCGC'C
Cuts at: 0 2003 3183
Size: 2003 1180
BbsI GAAGACnn'nnnn_
Cuts at: 0 428 1152 1472 2030 3183
Size: 428 724 320 558 1153
Fragments arranged by size:
1153 724 558 428 320
BbvCI CC'TCA_GC
Cuts at: 0 457 2885 3183
Size: 457 2428 298
Fragments arranged by size:
2428 457 298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3035 3069 3183
Size: 3035 34 114
Fragments arranged by size:
3035 114 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 282 3183
Size: 282 2901
BglI GCCn_nnn'nGGC
Cuts at: 0 2774 3074 3183
Size: 2774 300 109
Fragments arranged by size:
2774 300 109
BglII A'GATC_T
Cuts at: 0 2591 3183
Size: 2591 592
BlpI GC'TnA_GC
Cuts at: 0 2268 3064 3183
Size: 2268 796 119
Fragments arranged by size:
2268 796 119
Bme1580I G_kGCm'C
Cuts at: 0 1347 2131 3183
Size: 1347 784 1052
Fragments arranged by size:
1347 1052 784
BmgBI CAC'GTC
Cuts at: 0 1620 3183
Size: 1620 1563
BmrI ACTGGGnnnn_n'
Cuts at: 0 708 2385 2554 2874 3183
Size: 708 1677 169 320 309
Fragments arranged by size:
1677 708 320 309 169
BmtI G_CTAG'C
Cuts at: 0 575 3183
Size: 575 2608
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 233 2301 2646 3183
Size: 233 2068 345 537
Fragments arranged by size:
2068 537 345 233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 820 1081 1225 1273 1339 1744 2247
Size: 228 592 261 144 48 66 405 503
Cuts at: 2247 2452 2536 2728 2794 2899 3183
Size: 205 84 192 66 105 284
Fragments arranged by size:
592 503 405 284 261 228 205 192
144 105 84 66 66 48
Bpu10I CC'TnA_GC
Cuts at: 0 457 818 1001 1888 2671 2885 3183
Size: 457 361 183 887 783 214 298
Fragments arranged by size:
887 783 457 361 298 214 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1563 2862 3183
Size: 880 683 1299 321
Fragments arranged by size:
1299 880 683 321
BsaI GGTCTCn'nnnn_
Cuts at: 0 583 1702 1752 1950 3183
Size: 583 1119 50 198 1233
Fragments arranged by size:
1233 1119 583 198 50
BsaAI yAC'GTr
Cuts at: 0 1170 2735 3183
Size: 1170 1565 448
Fragments arranged by size:
1565 1170 448
BsaHI Gr'CG_yC
Cuts at: 0 1279 1469 2000 2096 3183
Size: 1279 190 531 96 1087
Fragments arranged by size:
1279 1087 531 190 96
BsaWI w'CCGG_w
Cuts at: 0 464 1442 3183
Size: 464 978 1741
Fragments arranged by size:
1741 978 464
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1855 1885 1909 1930 1939 1960 3183
Size: 1855 30 24 21 9 21 1223
Fragments arranged by size:
1855 1223 30 24 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 625 958 1444 1720 1879 1882 1938
Size: 214 411 333 486 276 159 3 56
Cuts at: 1938 3183
Size: 1245
Fragments arranged by size:
1245 486 411 333 276 214 159 56
3
BseYI C'CCAG_C
Cuts at: 0 111 170 823 938 1220 2685 2772 3183
Size: 111 59 653 115 282 1465 87 411
Fragments arranged by size:
1465 653 411 282 115 111 87 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1972 2086 2415 3183
Size: 1972 114 329 768
Fragments arranged by size:
1972 768 329 114
BsiHKAI G_wGCw'C
Cuts at: 0 497 845 896 1067 1387 1940 2041 2131
Size: 497 348 51 171 320 553 101 90
Cuts at: 2131 2468 2810 3183
Size: 337 342 373
Fragments arranged by size:
553 497 373 348 342 337 320 171
101 90 51
BsmI GAATG_Cn'
Cuts at: 0 787 3183
Size: 787 2396
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1476 3183
Size: 1476 1707
Bsp1286I G_dGCh'C
Cuts at: 0 497 824 845 896 1067 1347 1387 1406
Size: 497 327 21 51 171 280 40 19
Cuts at: 1406 1828 1940 2041 2131 2468 2779 2810 3183
Size: 422 112 101 90 337 311 31 373
Fragments arranged by size:
497 422 373 337 327 311 280 171
112 101 90 51 40 31 21 19
BspEI T'CCGG_A
Cuts at: 0 1442 3183
Size: 1442 1741
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 501 1243 1322 3183
Size: 501 742 79 1861
Fragments arranged by size:
1861 742 501 79
BsrBI CCG'CTC
Cuts at: 0 234 3183
Size: 234 2949
BsrDI GCAATG_nn'
Cuts at: 0 435 1919 3183
Size: 435 1484 1264
Fragments arranged by size:
1484 1264 435
BsrFI r'CCGG_y
Cuts at: 0 151 464 2768 3183
Size: 151 313 2304 415
Fragments arranged by size:
2304 415 313 151
BsrGI T'GTAC_A
Cuts at: 0 1301 3183
Size: 1301 1882
BssHII G'CGCG_C
Cuts at: 0 433 827 3183
Size: 433 394 2356
Fragments arranged by size:
2356 433 394
BstXI CCAn_nnnn'nTGG
Cuts at: 0 48 1038 1077 3183
Size: 48 990 39 2106
Fragments arranged by size:
2106 990 48 39
BstYI r'GATC_y
Cuts at: 0 125 369 1082 1781 1856 2325 2591 3183
Size: 125 244 713 699 75 469 266 592
Fragments arranged by size:
713 699 592 469 266 244 125 75
Bsu36I CC'TnA_GG
Cuts at: 0 917 2239 2530 2722 3183
Size: 917 1322 291 192 461
Fragments arranged by size:
1322 917 461 291 192
BtgI C'CryG_G
Cuts at: 0 557 635 776 956 1031 3183
Size: 557 78 141 180 75 2152
Fragments arranged by size:
2152 557 180 141 78 75
BtsI GCAGTG_nn'
Cuts at: 0 1292 1304 1783 2603 3183
Size: 1292 12 479 820 580
Fragments arranged by size:
1292 820 580 479 12
DraIII CAC_nnn'GTG
Cuts at: 0 301 687 1199 3183
Size: 301 386 512 1984
Fragments arranged by size:
1984 512 386 301
DrdI GACnn_nn'nnGTC
Cuts at: 0 3040 3183
Size: 3040 143
EarI CTCTTCn'nnn_
Cuts at: 0 721 745 1195 1723 2242 3183
Size: 721 24 450 528 519 941
Fragments arranged by size:
941 721 528 519 450 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 718 3183
Size: 718 2465
EcoICRI GAG'CTC
Cuts at: 0 843 894 3183
Size: 843 51 2289
Fragments arranged by size:
2289 843 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 228 789 820 1081 1225 1273 1339
Size: 94 134 561 31 261 144 48 66
Cuts at: 1339 1744 1824 1981 2215 2247 2266 2452 2536
Size: 405 80 157 234 32 19 186 84
Cuts at: 2536 2608 2728 2794 2899 3183
Size: 72 120 66 105 284
Fragments arranged by size:
561 405 284 261 234 186 157 144
134 120 105 94 84 80 72 66
66 48 32 31 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1426 1585 1702 1819 3183
Size: 1426 159 117 117 1364
Fragments arranged by size:
1426 1364 159 117 117
EcoO109I rG'GnC_Cy
Cuts at: 0 249 276 922 1343 1344 1437 1884 2280
Size: 249 27 646 421 1 93 447 396
Cuts at: 2280 2376 2691 2718 2868 3183
Size: 96 315 27 150 315
Fragments arranged by size:
646 447 421 396 315 315 249 150
96 93 27 27 1
EcoRI G'AATT_C
Cuts at: 0 1555 3183
Size: 1555 1628
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 367 1134 3183
Size: 367 767 2049
Fragments arranged by size:
2049 767 367
HaeII r_GCGC'y
Cuts at: 0 618 864 1968 1979 2003 3148 3183
Size: 618 246 1104 11 24 1145 35
Fragments arranged by size:
1145 1104 618 246 35 24 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2409 2441 3183
Size: 2409 32 742
Fragments arranged by size:
2409 742 32
HincII GTy'rAC
Cuts at: 0 329 453 1634 3183
Size: 329 124 1181 1549
Fragments arranged by size:
1549 1181 329 124
KasI G'GCGC_C
Cuts at: 0 1999 3183
Size: 1999 1184
MluI A'CGCG_T
Cuts at: 0 1643 3183
Size: 1643 1540
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2325 2474 3069 3183
Size: 2325 149 595 114
Fragments arranged by size:
2325 595 149 114
MslI CAynn'nnrTG
Cuts at: 0 1297 1762 1994 3183
Size: 1297 465 232 1189
Fragments arranged by size:
1297 1189 465 232
MspA1I CmG'CkG
Cuts at: 0 174 809 1251 1844 2119 2430 3183
Size: 174 635 442 593 275 311 753
Fragments arranged by size:
753 635 593 442 311 275 174
NaeI GCC'GGC
Cuts at: 0 2770 3183
Size: 2770 413
NarI GG'CG_CC
Cuts at: 0 2000 3183
Size: 2000 1183
NgoMIV G'CCGG_C
Cuts at: 0 2768 3183
Size: 2768 415
NheI G'CTAG_C
Cuts at: 0 571 3183
Size: 571 2612
NspI r_CATG'y
Cuts at: 0 335 792 1078 1308 3183
Size: 335 457 286 230 1875
Fragments arranged by size:
1875 457 335 286 230
PasI CC'CwG_GG
Cuts at: 0 131 2172 2979 3183
Size: 131 2041 807 204
Fragments arranged by size:
2041 807 204 131
PflMI CCAn_nnn'nTGG
Cuts at: 0 301 3183
Size: 301 2882
PfoI T'CCnGG_A
Cuts at: 0 836 1282 1550 1580 1859 2828 3183
Size: 836 446 268 30 279 969 355
Fragments arranged by size:
969 836 446 355 279 268 30
PmlI CAC'GTG
Cuts at: 0 2735 3183
Size: 2735 448
PpuMI rG'GwC_Cy
Cuts at: 0 249 276 922 1437 2280 2691 3183
Size: 249 27 646 515 843 411 492
Fragments arranged by size:
843 646 515 492 411 249 27
PshAI GACnn'nnGTC
Cuts at: 0 1278 3183
Size: 1278 1905
PspOMI G'GGCC_C
Cuts at: 0 1343 3183
Size: 1343 1840
PspXI vC'TCGA_Gb
Cuts at: 0 58 3183
Size: 58 3125
PstI C_TGCA'G
Cuts at: 0 757 1299 1820 1972 2292 2405 2569 2911
Size: 757 542 521 152 320 113 164 342
Cuts at: 2911 3183
Size: 272
Fragments arranged by size:
757 542 521 342 320 272 164 152
113
PvuII CAG'CTG
Cuts at: 0 174 809 1251 1844 2430 3183
Size: 174 635 442 593 586 753
Fragments arranged by size:
753 635 593 586 442 174
SacI G_AGCT'C
Cuts at: 0 845 896 3183
Size: 845 51 2287
Fragments arranged by size:
2287 845 51
SanDI GG'GwC_CC
Cuts at: 0 922 3183
Size: 922 2261
SapI GCTCTTCn'nnn_
Cuts at: 0 721 3183
Size: 721 2462
SexAI A'CCwGG_T
Cuts at: 0 278 297 3183
Size: 278 19 2886
Fragments arranged by size:
2886 278 19
SfcI C'TryA_G
Cuts at: 0 384 753 1295 1527 1816 1968 2007 2100
Size: 384 369 542 232 289 152 39 93
Cuts at: 2100 2288 2401 2565 2907 3183
Size: 188 113 164 342 276
Fragments arranged by size:
542 384 369 342 289 276 232 188
164 152 113 93 39
SfoI GGC'GCC
Cuts at: 0 2001 3183
Size: 2001 1182
SmlI C'TyrA_G
Cuts at: 0 58 895 1542 2841 3183
Size: 58 837 647 1299 342
Fragments arranged by size:
1299 837 647 342 58
SphI G_CATG'C
Cuts at: 0 792 3183
Size: 792 2391
StuI AGG'CCT
Cuts at: 0 205 3183
Size: 205 2978
StyI C'CwwG_G
Cuts at: 0 438 720 1388 1829 2951 3027 3183
Size: 438 282 668 441 1122 76 156
Fragments arranged by size:
1122 668 441 438 282 156 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 199 1212 1380 1651 2573 3016 3183
Size: 199 1013 168 271 922 443 167
Fragments arranged by size:
1013 922 443 271 199 168 167
TatI w'GTAC_w
Cuts at: 0 1301 1571 3183
Size: 1301 270 1612
Fragments arranged by size:
1612 1301 270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1930 1962 3183
Size: 1930 32 1221
Fragments arranged by size:
1930 1221 32
Tth111I GACn'n_nGTC
Cuts at: 0 394 3183
Size: 394 2789
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 203 1719 2481 3183
Size: 203 1516 762 702
Fragments arranged by size:
1516 762 702 203
XhoI C'TCGA_G
Cuts at: 0 58 3183
Size: 58 3125
XmnI GAAnn'nnTTC
Cuts at: 0 79 715 1495 3183
Size: 79 636 780 1688
Fragments arranged by size:
1688 780 636 79
ZraI GAC'GTC
Cuts at: 0 1280 1470 3183
Size: 1280 190 1713
Fragments arranged by size:
1713 1280 190
Enzymes that do cut:
AatII AcuI AfeI AflIII AgeI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BstXI BstYI Bsu36I BtgI BtsI
DraIII DrdI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII KasI MluI MmeI
MslI MspA1I NaeI NarI NgoMIV NheI NspI PasI
PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SanDI SapI SexAI SfcI SfoI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AhdI AloI AscI
AseI AsiSI BaeI BclI BfrBI BsaBI BsiEI BsiWI
BspHI BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI EaeI EagI EcoRV FseI FspI FspAI
HindIII HpaI KpnI MfeI MscI NcoI NdeI NotI
NruI NsiI PacI PciI PmeI PpiI PsiI PsrI
PvuI RsrII SacII SalI SbfI ScaI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XbaI
XmaI