(Linear) (Six-base) MAPSORT of: KIEE6622.seq  Check: 5013  from: 1  to: 918



KIEE6622.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:50  ..

AclI AA'CG_TT

Cuts at:      0     134     918 
   Size:        134     784
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26     653     918 
   Size:         26     627     265

  Fragments arranged by size: 

                 627     265      26
 
AfeI AGC'GCT

Cuts at:      0     226     918 
   Size:        226     692
 
ApoI r'AATT_y

Cuts at:      0     269     735     918 
   Size:        269     466     183

  Fragments arranged by size: 

                 466     269     183
 
AvaI C'yCGr_G

Cuts at:      0     703     918 
   Size:        703     215
 
BglI GCCn_nnn'nGGC

Cuts at:      0     792     918 
   Size:        792     126
 
BglII A'GATC_T

Cuts at:      0     195     918 
   Size:        195     723
 
BlpI GC'TnA_GC

Cuts at:      0     176     918 
   Size:        176     742
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     605     918 
   Size:        605     313
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56      91     151     202     918 
   Size:         56      35      60      51     716

  Fragments arranged by size: 

                 716      60      56      51      35
 
Bpu10I CC'TnA_GC

Cuts at:      0     263     918 
   Size:        263     655
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     575     584     918 
   Size:        575       9     334

  Fragments arranged by size: 

                 575     334       9
 
BseYI C'CCAG_C

Cuts at:      0     212     918 
   Size:        212     706
 
BsmI GAATG_Cn'

Cuts at:      0     308     918 
   Size:        308     610
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     294     918 
   Size:        294     624
 
BspHI T'CATG_A

Cuts at:      0     809     918 
   Size:        809     109
 
BsrBI CCG'CTC

Cuts at:      0     589     666     918 
   Size:        589      77     252

  Fragments arranged by size: 

                 589     252      77
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     412     918 
   Size:        412     506
 
BstYI r'GATC_y

Cuts at:      0     195     918 
   Size:        195     723
 
EaeI y'GGCC_r

Cuts at:      0     793     918 
   Size:        793     125
 
EarI CTCTTCn'nnn_

Cuts at:      0     362     918 
   Size:        362     556
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      26      56      91     151     202     653     918 
   Size:         26      30      35      60      51     451     265

  Fragments arranged by size: 

                 451     265      60      51      35      30      26
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     405     574     918 
   Size:        405     169     344

  Fragments arranged by size: 

                 405     344     169
 
HaeII r_GCGC'y

Cuts at:      0     228     918 
   Size:        228     690
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     102     134     661     693     918 
   Size:        102      32     527      32     225

  Fragments arranged by size: 

                 527     225     102      32      32
 
HindIII A'AGCT_T

Cuts at:      0     644     918 
   Size:        644     274
 
MspA1I CmG'CkG

Cuts at:      0     141     918 
   Size:        141     777
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      84     918 
   Size:         84     834
 
PfoI T'CCnGG_A

Cuts at:      0      68     918 
   Size:         68     850
 
PstI C_TGCA'G

Cuts at:      0     101     918 
   Size:        101     817
 
SbfI CC_TGCA'GG

Cuts at:      0     101     918 
   Size:        101     817
 
SfcI C'TryA_G

Cuts at:      0      97     918 
   Size:         97     821
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     792     918 
   Size:        792     126
 
SmlI C'TyrA_G

Cuts at:      0     590     599     918 
   Size:        590       9     319

  Fragments arranged by size: 

                 590     319       9
 
StyI C'CwwG_G

Cuts at:      0     659     918 
   Size:        659     259
 

 Enzymes that do cut:

     AclI     AcuI     AfeI     ApoI     AvaI     BglI    BglII     BlpI
     BplI     BpmI   Bpu10I    BpuEI    BseYI     BsmI    BsmBI    BspHI
    BsrBI   BstAPI    BstYI     EaeI     EarI  Eco57MI     FalI    HaeII
    Hin4I  HindIII   MspA1I    PflMI     PfoI     PstI     SbfI     SfcI
     SfiI     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI Bme1580I    BmgBI     BmrI
     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspEI    BspMI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI