(Linear) (Six-base) MAPSORT of: KIEE6561.seq  Check: 5650  from: 1  to: 480



KIEE6561.orf

 With 182 enzymes: SgfI * 

                        November 28, 2016 14:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     207     480 
   Size:        207     273
 
ApoI r'AATT_y

Cuts at:      0     308     480 
   Size:        308     172
 
BanI G'GyrC_C

Cuts at:      0     359     480 
   Size:        359     121
 
BglI GCCn_nnn'nGGC

Cuts at:      0     421     480 
   Size:        421      59
 
BmgBI CAC'GTC

Cuts at:      0      36     480 
   Size:         36     444
 
BsiEI CG_ry'CG

Cuts at:      0      71     480 
   Size:         71     409
 
BsmI GAATG_Cn'

Cuts at:      0     227     480 
   Size:        227     253
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      66     480 
   Size:         66     414
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     272     480 
   Size:        272     208
 
BsrDI GCAATG_nn'

Cuts at:      0      99     480 
   Size:         99     381
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     179     480 
   Size:        179     301
 
Bsu36I CC'TnA_GG

Cuts at:      0     138     480 
   Size:        138     342
 
EaeI y'GGCC_r

Cuts at:      0     341     480 
   Size:        341     139
 
EarI CTCTTCn'nnn_

Cuts at:      0     138     265     480 
   Size:        138     127     215

  Fragments arranged by size: 

                 215     138     127
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     207     480 
   Size:        207     273
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     271     480 
   Size:        271     209
 
MslI CAynn'nnrTG

Cuts at:      0     457     480 
   Size:        457      23
 
MspA1I CmG'CkG

Cuts at:      0     446     480 
   Size:        446      34
 
PstI C_TGCA'G

Cuts at:      0     188     480 
   Size:        188     292
 
PvuI CG_AT'CG

Cuts at:      0      71     480 
   Size:         71     409
 
SfcI C'TryA_G

Cuts at:      0     123     184     480 
   Size:        123      61     296

  Fragments arranged by size: 

                 296     123      61
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     421     480 
   Size:        421      59
 
StyI C'CwwG_G

Cuts at:      0     272     480 
   Size:        272     208
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     408     480 
   Size:        408      72
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     313     480 
   Size:        313     167
 

 Enzymes that do cut:

     AcuI     ApoI     BanI     BglI    BmgBI    BsiEI     BsmI    BsmBI
    BspMI    BsrDI    BstXI   Bsu36I     EaeI     EarI  Eco57MI    EcoNI
     MslI   MspA1I     PstI     PvuI     SfcI     SfiI     StyI    TaqII
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsiWI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI