Restriction map for
pF1KE4513
(Linear) (Six-base) MAPSORT of: KIEE4513.seq Check: 824 from: 1 to: 1626
KIEE4513.orf
With 182 enzymes: SgfI *
December 8, 2011 13:20 ..
AccI GT'mk_AC
Cuts at: 0 299 396 631 1626
Size: 299 97 235 995
Fragments arranged by size:
995 299 235 97
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 489 838 1260 1626
Size: 382 107 349 422 366
Fragments arranged by size:
422 382 366 349 107
AflII C'TTAA_G
Cuts at: 0 1128 1626
Size: 1128 498
AgeI A'CCGG_T
Cuts at: 0 1009 1626
Size: 1009 617
AhdI GACnn_n'nnGTC
Cuts at: 0 983 1626
Size: 983 643
AleI CACnn'nnGTG
Cuts at: 0 1204 1626
Size: 1204 422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 487 1626
Size: 487 1139
AlwNI CAG_nnn'CTG
Cuts at: 0 54 1626
Size: 54 1572
ApaI G_GGCC'C
Cuts at: 0 196 1626
Size: 196 1430
ApoI r'AATT_y
Cuts at: 0 1048 1626
Size: 1048 578
AvaI C'yCGr_G
Cuts at: 0 640 1450 1626
Size: 640 810 176
Fragments arranged by size:
810 640 176
BamHI G'GATC_C
Cuts at: 0 1586 1626
Size: 1586 40
BanI G'GyrC_C
Cuts at: 0 215 603 1626
Size: 215 388 1023
Fragments arranged by size:
1023 388 215
BanII G_rGCy'C
Cuts at: 0 196 413 890 1621 1626
Size: 196 217 477 731 5
Fragments arranged by size:
731 477 217 196 5
BbsI GAAGACnn'nnnn_
Cuts at: 0 525 735 834 1520 1626
Size: 525 210 99 686 106
Fragments arranged by size:
686 525 210 106 99
BciVI GTATCCnnnnn_n'
Cuts at: 0 950 1626
Size: 950 676
BglI GCCn_nnn'nGGC
Cuts at: 0 290 1192 1626
Size: 290 902 434
Fragments arranged by size:
902 434 290
BglII A'GATC_T
Cuts at: 0 113 911 1626
Size: 113 798 715
Fragments arranged by size:
798 715 113
BlpI GC'TnA_GC
Cuts at: 0 874 1626
Size: 874 752
Bme1580I G_kGCm'C
Cuts at: 0 196 1211 1626
Size: 196 1015 415
Fragments arranged by size:
1015 415 196
BmrI ACTGGGnnnn_n'
Cuts at: 0 79 369 693 1626
Size: 79 290 324 933
Fragments arranged by size:
933 324 290 79
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 889 1078 1607 1626
Size: 889 189 529 19
Fragments arranged by size:
889 529 189 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1605 1626
Size: 1057 548 21
Fragments arranged by size:
1057 548 21
Bpu10I CC'TnA_GC
Cuts at: 0 574 1626
Size: 574 1052
BsaI GGTCTCn'nnnn_
Cuts at: 0 416 1474 1626
Size: 416 1058 152
Fragments arranged by size:
1058 416 152
BsaBI GATnn'nnATC
Cuts at: 0 1263 1626
Size: 1263 363
BsaWI w'CCGG_w
Cuts at: 0 493 953 1009 1626
Size: 493 460 56 617
Fragments arranged by size:
617 493 460 56
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 59 89 148 178 398 428 1626
Size: 59 30 59 30 220 30 1198
Fragments arranged by size:
1198 220 59 59 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 898 1438 1626
Size: 898 540 188
Fragments arranged by size:
898 540 188
BseYI C'CCAG_C
Cuts at: 0 293 1101 1626
Size: 293 808 525
Fragments arranged by size:
808 525 293
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 1626
Size: 518 1108
BsiHKAI G_wGCw'C
Cuts at: 0 185 580 890 1626
Size: 185 395 310 736
Fragments arranged by size:
736 395 310 185
Bsp1286I G_dGCh'C
Cuts at: 0 185 196 413 580 890 1211 1621 1626
Size: 185 11 217 167 310 321 410 5
Fragments arranged by size:
410 321 310 217 185 167 11 5
BspEI T'CCGG_A
Cuts at: 0 953 1626
Size: 953 673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 113 382 469 479 908 1626
Size: 113 269 87 10 429 718
Fragments arranged by size:
718 429 269 113 87 10
BsrFI r'CCGG_y
Cuts at: 0 1009 1142 1626
Size: 1009 133 484
Fragments arranged by size:
1009 484 133
BsrGI T'GTAC_A
Cuts at: 0 228 1626
Size: 228 1398
BssSI C'ACGA_G
Cuts at: 0 412 1626
Size: 412 1214
BstXI CCAn_nnnn'nTGG
Cuts at: 0 528 1626
Size: 528 1098
BstYI r'GATC_y
Cuts at: 0 14 113 911 1586 1626
Size: 14 99 798 675 40
Fragments arranged by size:
798 675 99 40 14
BstZ17I GTA'TAC
Cuts at: 0 397 1626
Size: 397 1229
BtgI C'CryG_G
Cuts at: 0 188 272 1626
Size: 188 84 1354
Fragments arranged by size:
1354 188 84
BtsI GCAGTG_nn'
Cuts at: 0 1464 1626
Size: 1464 162
DrdI GACnn_nn'nnGTC
Cuts at: 0 771 1626
Size: 771 855
EaeI y'GGCC_r
Cuts at: 0 92 441 660 920 1626
Size: 92 349 219 260 706
Fragments arranged by size:
706 349 260 219 92
EarI CTCTTCn'nnn_
Cuts at: 0 854 1291 1474 1626
Size: 854 437 183 152
Fragments arranged by size:
854 437 183 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 224 1626
Size: 224 1402
EcoICRI GAG'CTC
Cuts at: 0 888 1626
Size: 888 738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 489 838 1057 1260 1605 1626
Size: 382 107 349 219 203 345 21
Fragments arranged by size:
382 349 345 219 203 107 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 904 1581 1626
Size: 904 677 45
Fragments arranged by size:
904 677 45
EcoO109I rG'GnC_Cy
Cuts at: 0 172 813 1626
Size: 172 641 813
Fragments arranged by size:
813 641 172
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 969 1001 1172 1204 1473 1505 1626
Size: 969 32 171 32 269 32 121
Fragments arranged by size:
969 269 171 121 32 32 32
HincII GTy'rAC
Cuts at: 0 632 719 1059 1626
Size: 632 87 340 567
Fragments arranged by size:
632 567 340 87
HpaI GTT'AAC
Cuts at: 0 719 1626
Size: 719 907
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 1626
Size: 377 1249
MscI TGG'CCA
Cuts at: 0 94 662 922 1626
Size: 94 568 260 704
Fragments arranged by size:
704 568 260 94
MslI CAynn'nnrTG
Cuts at: 0 526 759 1204 1626
Size: 526 233 445 422
Fragments arranged by size:
526 445 422 233
MspA1I CmG'CkG
Cuts at: 0 488 1162 1280 1383 1626
Size: 488 674 118 103 243
Fragments arranged by size:
674 488 243 118 103
NaeI GCC'GGC
Cuts at: 0 1144 1626
Size: 1144 482
NcoI C'CATG_G
Cuts at: 0 188 272 1626
Size: 188 84 1354
Fragments arranged by size:
1354 188 84
NgoMIV G'CCGG_C
Cuts at: 0 1142 1626
Size: 1142 484
NspI r_CATG'y
Cuts at: 0 61 1626
Size: 61 1565
PflMI CCAn_nnn'nTGG
Cuts at: 0 782 1626
Size: 782 844
PfoI T'CCnGG_A
Cuts at: 0 17 1626
Size: 17 1609
PspOMI G'GGCC_C
Cuts at: 0 192 1626
Size: 192 1434
PstI C_TGCA'G
Cuts at: 0 113 466 716 905 1626
Size: 113 353 250 189 721
Fragments arranged by size:
721 353 250 189 113
PvuII CAG'CTG
Cuts at: 0 1280 1626
Size: 1280 346
SacI G_AGCT'C
Cuts at: 0 890 1626
Size: 890 736
SalI G'TCGA_C
Cuts at: 0 630 1626
Size: 630 996
SfcI C'TryA_G
Cuts at: 0 109 462 712 901 1023 1594 1626
Size: 109 353 250 189 122 571 32
Fragments arranged by size:
571 353 250 189 122 109 32
SmlI C'TyrA_G
Cuts at: 0 1128 1626
Size: 1128 498
StuI AGG'CCT
Cuts at: 0 1576 1613 1626
Size: 1576 37 13
Fragments arranged by size:
1576 37 13
StyI C'CwwG_G
Cuts at: 0 175 188 272 311 917 923 1211 1626
Size: 175 13 84 39 606 6 288 415
Fragments arranged by size:
606 415 288 175 84 39 13 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 168 277 591 1027 1626
Size: 168 109 314 436 599
Fragments arranged by size:
599 436 314 168 109
TatI w'GTAC_w
Cuts at: 0 228 932 1626
Size: 228 704 694
Fragments arranged by size:
704 694 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 148 180 328 360 398 430 1358 1390
Size: 148 32 148 32 38 32 928 32
Cuts at: 1390 1626
Size: 236
Fragments arranged by size:
928 236 148 148 38 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 783 1081 1626
Size: 783 298 545
Fragments arranged by size:
783 545 298
Enzymes that do cut:
AccI AcuI AflII AgeI AhdI AleI AlfI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbsI BciVI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspMI BsrFI BsrGI BssSI BstXI BstYI
BstZ17I BtgI BtsI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I Hin4I HincII HpaI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PflMI PfoI
PspOMI PstI PvuII SacI SalI SfcI SmlI StuI
StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AloI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI
BclI BfrBI BmgBI BmtI BpuEI BsaAI BsaHI BsiEI
BsiWI BsmI BsmBI BspHI BsrBI BsrDI BssHII BstAPI
BstBI BstEII Bsu36I BtgZI ClaI CspCI DraI DraIII
EagI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII KasI KpnI MfeI MluI NarI NdeI NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI XmaI XmnI ZraI