(Linear) (Six-base) MAPSORT of: KIEE4446.seq  Check: 8826  from: 1  to: 1383



KIEE4446.orf

 With 182 enzymes: SgfI * 

                        October 12, 2018 16:45  ..

AfeI AGC'GCT

Cuts at:      0    1105    1383 
   Size:       1105     278
 
AflIII A'CryG_T

Cuts at:      0    1002    1075    1383 
   Size:       1002      73     308

  Fragments arranged by size: 

                1002     308      73
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     706    1383 
   Size:        706     677
 
AlwNI CAG_nnn'CTG

Cuts at:      0     300     799    1234    1383 
   Size:        300     499     435     149

  Fragments arranged by size: 

                 499     435     300     149
 
ApoI r'AATT_y

Cuts at:      0     505     739    1383 
   Size:        505     234     644

  Fragments arranged by size: 

                 644     505     234
 
AseI AT'TA_AT

Cuts at:      0      83    1376    1383 
   Size:         83    1293       7

  Fragments arranged by size: 

                1293      83       7
 
BanI G'GyrC_C

Cuts at:      0     306     511    1383 
   Size:        306     205     872

  Fragments arranged by size: 

                 872     306     205
 
BanII G_rGCy'C

Cuts at:      0     824     907    1383 
   Size:        824      83     476

  Fragments arranged by size: 

                 824     476      83
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     238    1383 
   Size:        238    1145
 
BbvCI CC'TCA_GC

Cuts at:      0     668    1383 
   Size:        668     715
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     207     241    1383 
   Size:        207      34    1142

  Fragments arranged by size: 

                1142     207      34
 
BfrBI ATG'CAT

Cuts at:      0    1125    1383 
   Size:       1125     258
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1239    1383 
   Size:       1239     144
 
BlpI GC'TnA_GC

Cuts at:      0     818    1383 
   Size:        818     565
 
BmtI G_CTAG'C

Cuts at:      0     272    1383 
   Size:        272    1111
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     821    1383 
   Size:        296     525     562

  Fragments arranged by size: 

                 562     525     296
 
Bpu10I CC'TnA_GC

Cuts at:      0     668    1383 
   Size:        668     715
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     610    1020    1383 
   Size:        610     410     363

  Fragments arranged by size: 

                 610     410     363
 
BsaHI Gr'CG_yC

Cuts at:      0     962    1383 
   Size:        962     421
 
BseYI C'CCAG_C

Cuts at:      0     768    1383 
   Size:        768     615
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     646    1383 
   Size:        313     333     737

  Fragments arranged by size: 

                 737     333     313
 
BsiEI CG_ry'CG

Cuts at:      0    1092    1383 
   Size:       1092     291
 
BsiHKAI G_wGCw'C

Cuts at:      0     435    1383 
   Size:        435     948
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     499    1383 
   Size:        499     884
 
Bsp1286I G_dGCh'C

Cuts at:      0     435     824     907    1383 
   Size:        435     389      83     476

  Fragments arranged by size: 

                 476     435     389      83
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1121    1383 
   Size:       1121     262
 
BsrDI GCAATG_nn'

Cuts at:      0     869    1383 
   Size:        869     514
 
BsrFI r'CCGG_y

Cuts at:      0     754    1383 
   Size:        754     629
 
BssSI C'ACGA_G

Cuts at:      0     179    1383 
   Size:        179    1204
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1248    1360    1383 
   Size:       1248     112      23

  Fragments arranged by size: 

                1248     112      23
 
BstYI r'GATC_y

Cuts at:      0     718    1383 
   Size:        718     665
 
ClaI AT'CG_AT

Cuts at:      0     566    1383 
   Size:        566     817
 
EarI CTCTTCn'nnn_

Cuts at:      0      14     740    1383 
   Size:         14     726     643

  Fragments arranged by size: 

                 726     643      14
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     821    1383 
   Size:        296     525     562

  Fragments arranged by size: 

                 562     525     296
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1231    1383 
   Size:       1231     152
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     132    1383 
   Size:        132    1251
 
HaeII r_GCGC'y

Cuts at:      0    1107    1163    1383 
   Size:       1107      56     220

  Fragments arranged by size: 

                1107     220      56
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     794     826    1383 
   Size:        794      32     557

  Fragments arranged by size: 

                 794     557      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     755    1383 
   Size:        755     628
 
MslI CAynn'nnrTG

Cuts at:      0    1001    1130    1383 
   Size:       1001     129     253

  Fragments arranged by size: 

                1001     253     129
 
MspA1I CmG'CkG

Cuts at:      0     647    1383 
   Size:        647     736
 
NdeI CA'TA_TG

Cuts at:      0    1063    1383 
   Size:       1063     320
 
NheI G'CTAG_C

Cuts at:      0     268    1383 
   Size:        268    1115
 
NsiI A_TGCA'T

Cuts at:      0    1127    1383 
   Size:       1127     256
 
NspI r_CATG'y

Cuts at:      0     687    1006    1079    1129    1383 
   Size:        687     319      73      50     254

  Fragments arranged by size: 

                 687     319     254      73      50
 
PciI A'CATG_T

Cuts at:      0    1002    1075    1383 
   Size:       1002      73     308

  Fragments arranged by size: 

                1002     308      73
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1064    1383 
   Size:       1064     319
 
PfoI T'CCnGG_A

Cuts at:      0     714    1383 
   Size:        714     669
 
PvuI CG_AT'CG

Cuts at:      0    1092    1383 
   Size:       1092     291
 
SgrAI Cr'CCGG_yG

Cuts at:      0     754    1383 
   Size:        754     629
 
StyI C'CwwG_G

Cuts at:      0      59    1383 
   Size:         59    1324
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1120    1383 
   Size:       1120     263
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      66     447    1383 
   Size:         66     381     936

  Fragments arranged by size: 

                 936     381      66
 

 Enzymes that do cut:

     AfeI   AflIII     AlfI    AlwNI     ApoI     AseI     BanI    BanII
     BbsI    BbvCI     BcgI    BfrBI     BglI     BlpI     BmtI     BpmI
   Bpu10I     BsaI    BsaHI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspMI    BsrDI    BsrFI    BssSI   BstAPI    BstYI     ClaI
     EarI  Eco57MI EcoO109I     FalI    HaeII    Hin4I     MmeI     MslI
   MspA1I     NdeI     NheI     NsiI     NspI     PciI    PflMI     PfoI
     PvuI    SgrAI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BciVI     BclI    BglII Bme1580I
    BmgBI     BmrI     BplI    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI
    BseRI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrGI   BssHII
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI
     NcoI   NgoMIV     NotI     NruI     PacI     PasI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI