Restriction map for
pF1KE4281
(Linear) (Six-base) MAPSORT of: KIEE4281.seq Check: 3733 from: 1 to: 4506
KIEE4281.orf
With 182 enzymes: SgfI *
March 8, 2012 13:22 ..
AccI GT'mk_AC
Cuts at: 0 4285 4506
Size: 4285 221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 2060 2726 3245 3266 4420 4506
Size: 470 1590 666 519 21 1154 86
Fragments arranged by size:
1590 1154 666 519 470 86 21
AflII C'TTAA_G
Cuts at: 0 1627 1749 4506
Size: 1627 122 2757
Fragments arranged by size:
2757 1627 122
AflIII A'CryG_T
Cuts at: 0 709 1666 3751 4506
Size: 709 957 2085 755
Fragments arranged by size:
2085 957 755 709
AleI CACnn'nnGTG
Cuts at: 0 1968 4506
Size: 1968 2538
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1730 4506
Size: 1730 2776
AlwNI CAG_nnn'CTG
Cuts at: 0 1260 2326 2653 2912 3798 4506
Size: 1260 1066 327 259 886 708
Fragments arranged by size:
1260 1066 886 708 327 259
ApoI r'AATT_y
Cuts at: 0 266 330 532 1297 1319 1550 1883 2017
Size: 266 64 202 765 22 231 333 134
Cuts at: 2017 2218 2740 2838 3359 3847 4126 4506
Size: 201 522 98 521 488 279 380
Fragments arranged by size:
765 522 521 488 380 333 279 266
231 202 201 134 98 64 22
AseI AT'TA_AT
Cuts at: 0 1184 2092 4506
Size: 1184 908 2414
Fragments arranged by size:
2414 1184 908
AvaI C'yCGr_G
Cuts at: 0 3224 4506
Size: 3224 1282
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 718 751 1752 1785 4506
Size: 718 33 1001 33 2721
Fragments arranged by size:
2721 1001 718 33 33
BamHI G'GATC_C
Cuts at: 0 3255 4488 4506
Size: 3255 1233 18
Fragments arranged by size:
3255 1233 18
BanI G'GyrC_C
Cuts at: 0 2144 3228 4428 4506
Size: 2144 1084 1200 78
Fragments arranged by size:
2144 1200 1084 78
BanII G_rGCy'C
Cuts at: 0 2296 3999 4506
Size: 2296 1703 507
Fragments arranged by size:
2296 1703 507
BbsI GAAGACnn'nnnn_
Cuts at: 0 3576 4090 4506
Size: 3576 514 416
Fragments arranged by size:
3576 514 416
BbvCI CC'TCA_GC
Cuts at: 0 4460 4506
Size: 4460 46
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2574 2608 4506
Size: 2574 34 1898
Fragments arranged by size:
2574 1898 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3065 4506
Size: 3065 1441
BclI T'GATC_A
Cuts at: 0 207 405 522 3936 4506
Size: 207 198 117 3414 570
Fragments arranged by size:
3414 570 207 198 117
BfrBI ATG'CAT
Cuts at: 0 485 4506
Size: 485 4021
BglII A'GATC_T
Cuts at: 0 1512 4017 4506
Size: 1512 2505 489
Fragments arranged by size:
2505 1512 489
BlpI GC'TnA_GC
Cuts at: 0 169 4506
Size: 169 4337
BmrI ACTGGGnnnn_n'
Cuts at: 0 231 4506
Size: 231 4275
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3775 4013 4506
Size: 3775 238 493
Fragments arranged by size:
3775 493 238
Bpu10I CC'TnA_GC
Cuts at: 0 1213 4460 4506
Size: 1213 3247 46
Fragments arranged by size:
3247 1213 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2352 2408 4506
Size: 2352 56 2098
Fragments arranged by size:
2352 2098 56
BsaI GGTCTCn'nnnn_
Cuts at: 0 4248 4506
Size: 4248 258
BsaAI yAC'GTr
Cuts at: 0 3754 4161 4506
Size: 3754 407 345
Fragments arranged by size:
3754 407 345
BsaBI GATnn'nnATC
Cuts at: 0 620 1511 3254 3941 4506
Size: 620 891 1743 687 565
Fragments arranged by size:
1743 891 687 620 565
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 179 209 4346 4376 4506
Size: 179 30 4137 30 130
Fragments arranged by size:
4137 179 130 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1290 2267 4376 4506
Size: 1290 977 2109 130
Fragments arranged by size:
2109 1290 977 130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 704 859 4506
Size: 374 330 155 3647
Fragments arranged by size:
3647 374 330 155
BsiEI CG_ry'CG
Cuts at: 0 1906 4506
Size: 1906 2600
BsiHKAI G_wGCw'C
Cuts at: 0 1219 4506
Size: 1219 3287
BsmI GAATG_Cn'
Cuts at: 0 265 613 1488 2597 4506
Size: 265 348 875 1109 1909
Fragments arranged by size:
1909 1109 875 348 265
Bsp1286I G_dGCh'C
Cuts at: 0 1219 2296 3999 4506
Size: 1219 1077 1703 507
Fragments arranged by size:
1703 1219 1077 507
BspHI T'CATG_A
Cuts at: 0 3081 4506
Size: 3081 1425
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2205 4506
Size: 2205 2301
BsrDI GCAATG_nn'
Cuts at: 0 2182 2717 4450 4506
Size: 2182 535 1733 56
Fragments arranged by size:
2182 1733 535 56
BsrFI r'CCGG_y
Cuts at: 0 1905 4506
Size: 1905 2601
BsrGI T'GTAC_A
Cuts at: 0 392 1251 1687 2634 2991 4506
Size: 392 859 436 947 357 1515
Fragments arranged by size:
1515 947 859 436 392 357
BstBI TT'CG_AA
Cuts at: 0 3363 4506
Size: 3363 1143
BstYI r'GATC_y
Cuts at: 0 1512 2361 2376 3255 3591 3795 4017 4026
Size: 1512 849 15 879 336 204 222 9
Cuts at: 4026 4488 4506
Size: 462 18
Fragments arranged by size:
1512 879 849 462 336 222 204 18
15 9
BtgI C'CryG_G
Cuts at: 0 1345 4506
Size: 1345 3161
BtsI GCAGTG_nn'
Cuts at: 0 2421 2732 4336 4506
Size: 2421 311 1604 170
Fragments arranged by size:
2421 1604 311 170
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4361 4396 4421 4456 4506
Size: 4361 35 25 35 50
Fragments arranged by size:
4361 50 35 35 25
DraI TTT'AAA
Cuts at: 0 1959 3176 4506
Size: 1959 1217 1330
Fragments arranged by size:
1959 1330 1217
DraIII CAC_nnn'GTG
Cuts at: 0 3013 3699 4506
Size: 3013 686 807
Fragments arranged by size:
3013 807 686
EaeI y'GGCC_r
Cuts at: 0 1677 1743 1903 4240 4506
Size: 1677 66 160 2337 266
Fragments arranged by size:
2337 1677 266 160 66
EagI C'GGCC_G
Cuts at: 0 1903 4506
Size: 1903 2603
EarI CTCTTCn'nnn_
Cuts at: 0 1001 1055 1267 1457 2517 4052 4506
Size: 1001 54 212 190 1060 1535 454
Fragments arranged by size:
1535 1060 1001 454 212 190 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1209 4506
Size: 1209 3297
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 2060 2726 3245 3266 3775 4013 4420
Size: 470 1590 666 519 21 509 238 407
Cuts at: 4420 4506
Size: 86
Fragments arranged by size:
1590 666 519 509 470 407 238 86
21
EcoRV GAT'ATC
Cuts at: 0 1265 4506
Size: 1265 3241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1495 1807 3613 4506
Size: 1495 312 1806 893
Fragments arranged by size:
1806 1495 893 312
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 680 712 840 872 1600 1632 1903 1935
Size: 680 32 128 32 728 32 271 32
Cuts at: 1935 3406 3438 4155 4187 4210 4242 4267 4299
Size: 1471 32 717 32 23 32 25 32
Cuts at: 4299 4506
Size: 207
Fragments arranged by size:
1471 728 717 680 271 207 128 32
32 32 32 32 32 32 32 25
23
HincII GTy'rAC
Cuts at: 0 699 1788 4506
Size: 699 1089 2718
Fragments arranged by size:
2718 1089 699
HindIII A'AGCT_T
Cuts at: 0 1088 1586 2042 2579 4004 4506
Size: 1088 498 456 537 1425 502
Fragments arranged by size:
1425 1088 537 502 498 456
HpaI GTT'AAC
Cuts at: 0 1788 4506
Size: 1788 2718
MfeI C'AATT_G
Cuts at: 0 2516 2978 3182 4506
Size: 2516 462 204 1324
Fragments arranged by size:
2516 1324 462 204
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 75 716 1715 3028 4506
Size: 33 42 641 999 1313 1478
Fragments arranged by size:
1478 1313 999 641 42 33
MscI TGG'CCA
Cuts at: 0 1679 4242 4506
Size: 1679 2563 264
Fragments arranged by size:
2563 1679 264
MslI CAynn'nnrTG
Cuts at: 0 1699 1968 2879 3086 3518 4506
Size: 1699 269 911 207 432 988
Fragments arranged by size:
1699 988 911 432 269 207
MspA1I CmG'CkG
Cuts at: 0 358 2640 3808 3896 4464 4506
Size: 358 2282 1168 88 568 42
Fragments arranged by size:
2282 1168 568 358 88 42
NcoI C'CATG_G
Cuts at: 0 1345 4506
Size: 1345 3161
NsiI A_TGCA'T
Cuts at: 0 487 4506
Size: 487 4019
NspI r_CATG'y
Cuts at: 0 713 1670 4506
Size: 713 957 2836
Fragments arranged by size:
2836 957 713
PciI A'CATG_T
Cuts at: 0 709 1666 4506
Size: 709 957 2840
Fragments arranged by size:
2840 957 709
PflMI CCAn_nnn'nTGG
Cuts at: 0 3798 4506
Size: 3798 708
PfoI T'CCnGG_A
Cuts at: 0 3791 4079 4506
Size: 3791 288 427
Fragments arranged by size:
3791 427 288
PsiI TTA'TAA
Cuts at: 0 785 4506
Size: 785 3721
PvuII CAG'CTG
Cuts at: 0 358 2640 3808 4464 4506
Size: 358 2282 1168 656 42
Fragments arranged by size:
2282 1168 656 358 42
SfcI C'TryA_G
Cuts at: 0 3501 3986 4506
Size: 3501 485 520
Fragments arranged by size:
3501 520 485
SmaI CCC'GGG
Cuts at: 0 3226 4506
Size: 3226 1280
SmlI C'TyrA_G
Cuts at: 0 1627 1749 2331 2423 4506
Size: 1627 122 582 92 2083
Fragments arranged by size:
2083 1627 582 122 92
SpeI A'CTAG_T
Cuts at: 0 2538 4506
Size: 2538 1968
SspI AAT'ATT
Cuts at: 0 145 2498 3921 4506
Size: 145 2353 1423 585
Fragments arranged by size:
2353 1423 585 145
StuI AGG'CCT
Cuts at: 0 3447 4506
Size: 3447 1059
StyI C'CwwG_G
Cuts at: 0 1153 1345 2148 3387 4506
Size: 1153 192 803 1239 1119
Fragments arranged by size:
1239 1153 1119 803 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1958 3400 4506
Size: 1958 1442 1106
Fragments arranged by size:
1958 1442 1106
TatI w'GTAC_w
Cuts at: 0 218 392 1251 1687 2634 2991 4506
Size: 218 174 859 436 947 357 1515
Fragments arranged by size:
1515 947 859 436 357 218 174
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1345 1377 4400 4432 4506
Size: 1345 32 3023 32 74
Fragments arranged by size:
3023 1345 74 32 32
XbaI T'CTAG_A
Cuts at: 0 1056 2814 4506
Size: 1056 1758 1692
Fragments arranged by size:
1758 1692 1056
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1343 2515 4506
Size: 1343 1172 1991
Fragments arranged by size:
1991 1343 1172
XmaI C'CCGG_G
Cuts at: 0 3224 4506
Size: 3224 1282
XmnI GAAnn'nnTTC
Cuts at: 0 1319 1466 1619 2540 3513 4097 4506
Size: 1319 147 153 921 973 584 409
Fragments arranged by size:
1319 973 921 584 409 153 147
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlfI AlwNI ApoI
AseI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BstBI BstYI BtgI BtsI CspCI DraI DraIII
EaeI EagI EarI EciI Eco57MI EcoRV FalI Hin4I
HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I
NcoI NsiI NspI PciI PflMI PfoI PsiI PvuII
SfcI SmaI SmlI SpeI SspI StuI StyI TaqII
TatI TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AloI
ApaI ApaLI AscI AsiSI AvrII BbeI BglI Bme1580I
BmgBI BmtI BplI BsaHI BsaWI BseYI BsiWI BsmBI
BspEI BsrBI BssHII BssSI BstAPI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI DrdI EcoICRI EcoNI EcoO109I EcoRI
FseI FspI FspAI HaeII KasI KpnI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SphI SrfI SwaI Tth111I XhoI ZraI