Restriction map for
pF1KE4271
(Linear) (Six-base) MAPSORT of: KIEE4271.seq Check: 2232 from: 1 to: 3411
KIEE4271.orf
With 182 enzymes: SgfI *
March 15, 2013 17:10 ..
AccI GT'mk_AC
Cuts at: 0 88 852 2289 3411
Size: 88 764 1437 1122
Fragments arranged by size:
1437 1122 764 88
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 3411
Size: 569 2842
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 749 3411
Size: 749 2662
AlwNI CAG_nnn'CTG
Cuts at: 0 52 160 3411
Size: 52 108 3251
Fragments arranged by size:
3251 108 52
ApoI r'AATT_y
Cuts at: 0 415 633 1355 1534 1569 1597 1951 2021
Size: 415 218 722 179 35 28 354 70
Cuts at: 2021 2153 2437 3242 3411
Size: 132 284 805 169
Fragments arranged by size:
805 722 415 354 284 218 179 169
132 70 35 28
AseI AT'TA_AT
Cuts at: 0 569 1610 3286 3411
Size: 569 1041 1676 125
Fragments arranged by size:
1676 1041 569 125
AvaI C'yCGr_G
Cuts at: 0 800 3411
Size: 800 2611
BamHI G'GATC_C
Cuts at: 0 3123 3411
Size: 3123 288
BanI G'GyrC_C
Cuts at: 0 2588 3411
Size: 2588 823
BanII G_rGCy'C
Cuts at: 0 809 2710 3411
Size: 809 1901 701
Fragments arranged by size:
1901 809 701
BbsI GAAGACnn'nnnn_
Cuts at: 0 511 3411
Size: 511 2900
BfrBI ATG'CAT
Cuts at: 0 2184 3339 3411
Size: 2184 1155 72
Fragments arranged by size:
2184 1155 72
BglI GCCn_nnn'nGGC
Cuts at: 0 175 3411
Size: 175 3236
BlpI GC'TnA_GC
Cuts at: 0 45 2522 3411
Size: 45 2477 889
Fragments arranged by size:
2477 889 45
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 3257 3411
Size: 24 3233 154
Fragments arranged by size:
3233 154 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3001 3295 3411
Size: 3001 294 116
Fragments arranged by size:
3001 294 116
BsaI GGTCTCn'nnnn_
Cuts at: 0 258 3411
Size: 258 3153
BsaAI yAC'GTr
Cuts at: 0 234 1846 3411
Size: 234 1612 1565
Fragments arranged by size:
1612 1565 234
BsaBI GATnn'nnATC
Cuts at: 0 1031 2737 3411
Size: 1031 1706 674
Fragments arranged by size:
1706 1031 674
BsaWI w'CCGG_w
Cuts at: 0 2003 2683 3411
Size: 2003 680 728
Fragments arranged by size:
2003 728 680
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1013 1043 3411
Size: 1013 30 2368
Fragments arranged by size:
2368 1013 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 102 105 2023 3385 3411
Size: 102 3 1918 1362 26
Fragments arranged by size:
1918 1362 102 26 3
BseYI C'CCAG_C
Cuts at: 0 189 198 218 1742 2610 3411
Size: 189 9 20 1524 868 801
Fragments arranged by size:
1524 868 801 189 20 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 1198 1363 2207 3411
Size: 146 1052 165 844 1204
Fragments arranged by size:
1204 1052 844 165 146
BsiEI CG_ry'CG
Cuts at: 0 170 179 3411
Size: 170 9 3232
Fragments arranged by size:
3232 170 9
BsiHKAI G_wGCw'C
Cuts at: 0 809 2710 2947 3411
Size: 809 1901 237 464
Fragments arranged by size:
1901 809 464 237
BsmI GAATG_Cn'
Cuts at: 0 540 2186 3411
Size: 540 1646 1225
Fragments arranged by size:
1646 1225 540
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1710 3411
Size: 1710 1701
Bsp1286I G_dGCh'C
Cuts at: 0 809 2710 2947 3411
Size: 809 1901 237 464
Fragments arranged by size:
1901 809 464 237
BspEI T'CCGG_A
Cuts at: 0 2003 3411
Size: 2003 1408
BspHI T'CATG_A
Cuts at: 0 627 2949 3411
Size: 627 2322 462
Fragments arranged by size:
2322 627 462
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1977 3362 3411
Size: 1977 1385 49
Fragments arranged by size:
1977 1385 49
BsrDI GCAATG_nn'
Cuts at: 0 2963 3411
Size: 2963 448
BsrGI T'GTAC_A
Cuts at: 0 2550 3411
Size: 2550 861
BstAPI GCAn_nnn'nTGC
Cuts at: 0 160 535 793 3411
Size: 160 375 258 2618
Fragments arranged by size:
2618 375 258 160
BstBI TT'CG_AA
Cuts at: 0 556 3411
Size: 556 2855
BstEII G'GTnAC_C
Cuts at: 0 1737 3411
Size: 1737 1674
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2300 3411
Size: 2300 1111
BstYI r'GATC_y
Cuts at: 0 2174 3123 3411
Size: 2174 949 288
Fragments arranged by size:
2174 949 288
BstZ17I GTA'TAC
Cuts at: 0 853 2290 3411
Size: 853 1437 1121
Fragments arranged by size:
1437 1121 853
BtsI GCAGTG_nn'
Cuts at: 0 2415 2477 3411
Size: 2415 62 934
Fragments arranged by size:
2415 934 62
DraI TTT'AAA
Cuts at: 0 1575 2109 3411
Size: 1575 534 1302
Fragments arranged by size:
1575 1302 534
DraIII CAC_nnn'GTG
Cuts at: 0 2595 3072 3411
Size: 2595 477 339
Fragments arranged by size:
2595 477 339
EaeI y'GGCC_r
Cuts at: 0 167 176 2510 3411
Size: 167 9 2334 901
Fragments arranged by size:
2334 901 167 9
EagI C'GGCC_G
Cuts at: 0 167 176 3411
Size: 167 9 3235
Fragments arranged by size:
3235 167 9
EarI CTCTTCn'nnn_
Cuts at: 0 256 2928 3407 3411
Size: 256 2672 479 4
Fragments arranged by size:
2672 479 256 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3023 3411
Size: 3023 388
EcoICRI GAG'CTC
Cuts at: 0 807 2708 3411
Size: 807 1901 703
Fragments arranged by size:
1901 807 703
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 569 3257 3411
Size: 24 545 2688 154
Fragments arranged by size:
2688 545 154 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1187 2512 3411
Size: 1187 1325 899
Fragments arranged by size:
1325 1187 899
EcoRI G'AATT_C
Cuts at: 0 415 1355 1534 3411
Size: 415 940 179 1877
Fragments arranged by size:
1877 940 415 179
EcoRV GAT'ATC
Cuts at: 0 513 3411
Size: 513 2898
HaeII r_GCGC'y
Cuts at: 0 152 187 196 1315 3411
Size: 152 35 9 1119 2096
Fragments arranged by size:
2096 1119 152 35 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 447 479 1774 1806 1821 1853 2931 2963
Size: 447 32 1295 32 15 32 1078 32
Cuts at: 2963 2989 3021 3083 3115 3411
Size: 26 32 62 32 296
Fragments arranged by size:
1295 1078 447 296 62 32 32 32
32 32 32 26 15
HincII GTy'rAC
Cuts at: 0 1443 1479 2493 3411
Size: 1443 36 1014 918
Fragments arranged by size:
1443 1014 918 36
HpaI GTT'AAC
Cuts at: 0 1479 3411
Size: 1479 1932
MfeI C'AATT_G
Cuts at: 0 2783 3411
Size: 2783 628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 2292 3411
Size: 336 1956 1119
Fragments arranged by size:
1956 1119 336
MscI TGG'CCA
Cuts at: 0 2512 3411
Size: 2512 899
MslI CAynn'nnrTG
Cuts at: 0 712 731 2812 3411
Size: 712 19 2081 599
Fragments arranged by size:
2081 712 599 19
MspA1I CmG'CkG
Cuts at: 0 157 166 175 222 3411
Size: 157 9 9 47 3189
Fragments arranged by size:
3189 157 47 9 9
NotI GC'GGCC_GC
Cuts at: 0 167 176 3411
Size: 167 9 3235
Fragments arranged by size:
3235 167 9
NsiI A_TGCA'T
Cuts at: 0 2186 3341 3411
Size: 2186 1155 70
Fragments arranged by size:
2186 1155 70
PfoI T'CCnGG_A
Cuts at: 0 979 1214 2384 3234 3411
Size: 979 235 1170 850 177
Fragments arranged by size:
1170 979 850 235 177
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1781 1813 3411
Size: 1781 32 1598
Fragments arranged by size:
1781 1598 32
PstI C_TGCA'G
Cuts at: 0 156 165 470 939 1265 2410 2542 2772
Size: 156 9 305 469 326 1145 132 230
Cuts at: 2772 2826 3359 3411
Size: 54 533 52
Fragments arranged by size:
1145 533 469 326 305 230 156 132
54 52 9
PvuII CAG'CTG
Cuts at: 0 222 3411
Size: 222 3189
RsrII CG'GwC_CG
Cuts at: 0 2006 3411
Size: 2006 1405
SacI G_AGCT'C
Cuts at: 0 809 2710 3411
Size: 809 1901 701
Fragments arranged by size:
1901 809 701
SexAI A'CCwGG_T
Cuts at: 0 134 3068 3411
Size: 134 2934 343
Fragments arranged by size:
2934 343 134
SfcI C'TryA_G
Cuts at: 0 152 161 239 466 935 1261 1642 1875
Size: 152 9 78 227 469 326 381 233
Cuts at: 1875 2406 2538 2768 2822 3355 3411
Size: 531 132 230 54 533 56
Fragments arranged by size:
533 531 469 381 326 233 230 227
152 132 78 56 54 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 175 3411
Size: 175 3236
SmlI C'TyrA_G
Cuts at: 0 2980 3310 3411
Size: 2980 330 101
Fragments arranged by size:
2980 330 101
SnaBI TAC'GTA
Cuts at: 0 1846 3411
Size: 1846 1565
SspI AAT'ATT
Cuts at: 0 813 3411
Size: 813 2598
StyI C'CwwG_G
Cuts at: 0 2513 2909 3411
Size: 2513 396 502
Fragments arranged by size:
2513 502 396
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1725 2686 2806 3040 3411
Size: 1725 961 120 234 371
Fragments arranged by size:
1725 961 371 234 120
TatI w'GTAC_w
Cuts at: 0 496 2550 2848 3411
Size: 496 2054 298 563
Fragments arranged by size:
2054 563 496 298
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 893 925 1013 1045 3015 3047 3197 3229
Size: 893 32 88 32 1970 32 150 32
Cuts at: 3229 3411
Size: 182
Fragments arranged by size:
1970 893 182 150 88 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 2430 3411
Size: 2430 981
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 142 3411
Size: 142 3269
XmnI GAAnn'nnTTC
Cuts at: 0 415 3411
Size: 415 2996
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApoI AseI AvaI BamHI
BanI BanII BbsI BfrBI BglI BlpI BpmI BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtsI DraI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI EcoRV HaeII Hin4I HincII HpaI
MfeI MmeI MscI MslI MspA1I NotI NsiI PfoI
PsrI PstI PvuII RsrII SacI SexAI SfcI SfiI
SmlI SnaBI SspI StyI TaqII TatI TstI XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BclI BglII Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BsaHI BsiWI BsrBI
BsrFI BssHII BssSI Bsu36I BtgI BtgZI ClaI CspCI
DrdI EcoO109I FalI FseI FspI FspAI HindIII KasI
KpnI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NruI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI SacII
SalI SanDI SapI SbfI ScaI SfoI SgfI SgrAI
SmaI SpeI SphI SrfI StuI SwaI Tth111I XhoI
XmaI ZraI