Restriction map for
pF1KE3935
(Linear) (Six-base) MAPSORT of: KIEE3935.seq Check: 6197 from: 1 to: 1398
KIEE3935.orf
With 182 enzymes: SgfI *
November 18, 2016 17:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 711 1398
Size: 711 687
AccI GT'mk_AC
Cuts at: 0 164 1398
Size: 164 1234
Acc65I G'GTAC_C
Cuts at: 0 495 1398
Size: 495 903
AclI AA'CG_TT
Cuts at: 0 331 1398
Size: 331 1067
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 467 488 1398
Size: 375 92 21 910
Fragments arranged by size:
910 375 92 21
AlwNI CAG_nnn'CTG
Cuts at: 0 586 622 703 802 1398
Size: 586 36 81 99 596
Fragments arranged by size:
596 586 99 81 36
ApoI r'AATT_y
Cuts at: 0 40 367 1398
Size: 40 327 1031
Fragments arranged by size:
1031 327 40
AvaI C'yCGr_G
Cuts at: 0 1244 1301 1398
Size: 1244 57 97
Fragments arranged by size:
1244 97 57
BanI G'GyrC_C
Cuts at: 0 495 1398
Size: 495 903
BbsI GAAGACnn'nnnn_
Cuts at: 0 737 1246 1398
Size: 737 509 152
Fragments arranged by size:
737 509 152
BbvCI CC'TCA_GC
Cuts at: 0 890 1398
Size: 890 508
BciVI GTATCCnnnnn_n'
Cuts at: 0 800 1398
Size: 800 598
BclI T'GATC_A
Cuts at: 0 522 1398
Size: 522 876
BmrI ACTGGGnnnn_n'
Cuts at: 0 776 812 1398
Size: 776 36 586
Fragments arranged by size:
776 586 36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 710 1349 1398
Size: 710 639 49
Fragments arranged by size:
710 639 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 1313 1398
Size: 680 633 85
Fragments arranged by size:
680 633 85
Bpu10I CC'TnA_GC
Cuts at: 0 644 890 1183 1398
Size: 644 246 293 215
Fragments arranged by size:
644 293 246 215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 756 1398
Size: 756 642
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1334 1364 1398
Size: 1334 30 34
Fragments arranged by size:
1334 34 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 330 454 577 1398
Size: 330 124 123 821
Fragments arranged by size:
821 330 124 123
BseYI C'CCAG_C
Cuts at: 0 1311 1398
Size: 1311 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1372 1398
Size: 1372 26
BsiEI CG_ry'CG
Cuts at: 0 1266 1398
Size: 1266 132
BspHI T'CATG_A
Cuts at: 0 1044 1398
Size: 1044 354
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 711 1046 1398
Size: 711 335 352
Fragments arranged by size:
711 352 335
Bsu36I CC'TnA_GG
Cuts at: 0 347 434 488 671 1398
Size: 347 87 54 183 727
Fragments arranged by size:
727 347 183 87 54
BtgI C'CryG_G
Cuts at: 0 123 1338 1390 1398
Size: 123 1215 52 8
Fragments arranged by size:
1215 123 52 8
BtsI GCAGTG_nn'
Cuts at: 0 61 1398
Size: 61 1337
DraIII CAC_nnn'GTG
Cuts at: 0 784 1398
Size: 784 614
EarI CTCTTCn'nnn_
Cuts at: 0 117 679 1030 1226 1398
Size: 117 562 351 196 172
Fragments arranged by size:
562 351 196 172 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 467 488 680 1313 1398
Size: 375 92 21 192 633 85
Fragments arranged by size:
633 375 192 92 85 21
EcoO109I rG'GnC_Cy
Cuts at: 0 75 943 1288 1297 1330 1398
Size: 75 868 345 9 33 68
Fragments arranged by size:
868 345 75 68 33 9
EcoRV GAT'ATC
Cuts at: 0 387 1398
Size: 387 1011
FspI TGC'GCA
Cuts at: 0 1108 1398
Size: 1108 290
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 798 830 1199 1231 1398
Size: 798 32 369 32 167
Fragments arranged by size:
798 369 167 32 32
KpnI G_GTAC'C
Cuts at: 0 499 1398
Size: 499 899
MslI CAynn'nnrTG
Cuts at: 0 239 1398
Size: 239 1159
MspA1I CmG'CkG
Cuts at: 0 894 1398
Size: 894 504
NcoI C'CATG_G
Cuts at: 0 123 1398
Size: 123 1275
NspI r_CATG'y
Cuts at: 0 87 1398
Size: 87 1311
PflMI CCAn_nnn'nTGG
Cuts at: 0 282 784 802 1366 1398
Size: 282 502 18 564 32
Fragments arranged by size:
564 502 282 32 18
PpuMI rG'GwC_Cy
Cuts at: 0 943 1288 1398
Size: 943 345 110
Fragments arranged by size:
943 345 110
PspXI vC'TCGA_Gb
Cuts at: 0 1244 1301 1398
Size: 1244 57 97
Fragments arranged by size:
1244 97 57
PstI C_TGCA'G
Cuts at: 0 708 1398
Size: 708 690
SanDI GG'GwC_CC
Cuts at: 0 943 1398
Size: 943 455
SfcI C'TryA_G
Cuts at: 0 704 1398
Size: 704 694
SmlI C'TyrA_G
Cuts at: 0 735 1244 1301 1398
Size: 735 509 57 97
Fragments arranged by size:
735 509 97 57
SspI AAT'ATT
Cuts at: 0 927 1398
Size: 927 471
StyI C'CwwG_G
Cuts at: 0 123 1398
Size: 123 1275
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 795 1280 1303 1398
Size: 795 485 23 95
Fragments arranged by size:
795 485 95 23
TatI w'GTAC_w
Cuts at: 0 723 1398
Size: 723 675
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1082 1114 1334 1355 1366 1387 1398
Size: 1082 32 220 21 11 21 11
Fragments arranged by size:
1082 220 32 21 21 11 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1027 1398
Size: 1027 371
XhoI C'TCGA_G
Cuts at: 0 1244 1301 1398
Size: 1244 57 97
Fragments arranged by size:
1244 97 57
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AlwNI ApoI AvaI
BanI BbsI BbvCI BciVI BclI BmrI BplI BpmI
Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiEI BspHI
BspMI Bsu36I BtgI BtsI DraIII EarI Eco57MI EcoO109I
EcoRV FspI Hin4I KpnI MslI MspA1I NcoI NspI
PflMI PpuMI PspXI PstI SanDI SfcI SmlI SspI
StyI TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AatII AfeI AflII AflIII AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbeI BcgI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmtI BsaI BsaAI BsaBI BsaHI BsaWI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgZI ClaI CspCI DraI DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRI FalI FseI FspAI
HaeII HincII HindIII HpaI KasI MfeI MluI MmeI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI Tth111I XbaI XmaI XmnI ZraI