Restriction map for
pF1KE3637
(Linear) (Six-base) MAPSORT of: KIEE3637.seq Check: 8912 from: 1 to: 1554
KIEE3637.orf
With 182 enzymes: SgfI *
August 9, 2016 17:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1500 1554
Size: 1500 54
AfeI AGC'GCT
Cuts at: 0 178 886 1554
Size: 178 708 668
Fragments arranged by size:
708 668 178
AhdI GACnn_n'nnGTC
Cuts at: 0 111 1554
Size: 111 1443
AlwNI CAG_nnn'CTG
Cuts at: 0 544 1554
Size: 544 1010
ApaI G_GGCC'C
Cuts at: 0 12 423 551 742 1554
Size: 12 411 128 191 812
Fragments arranged by size:
812 411 191 128 12
AvaI C'yCGr_G
Cuts at: 0 290 902 1554
Size: 290 612 652
Fragments arranged by size:
652 612 290
BamHI G'GATC_C
Cuts at: 0 119 1554
Size: 119 1435
BanI G'GyrC_C
Cuts at: 0 783 1554
Size: 783 771
BanII G_rGCy'C
Cuts at: 0 12 423 458 484 551 584 742 1031
Size: 12 411 35 26 67 33 158 289
Cuts at: 1031 1187 1298 1514 1554
Size: 156 111 216 40
Fragments arranged by size:
411 289 216 158 156 111 67 40
35 33 26 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 64 1554
Size: 64 1490
BbvCI CC'TCA_GC
Cuts at: 0 632 1554
Size: 632 922
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 227 261 1554
Size: 227 34 1293
Fragments arranged by size:
1293 227 34
BglII A'GATC_T
Cuts at: 0 1451 1554
Size: 1451 103
Bme1580I G_kGCm'C
Cuts at: 0 12 423 551 742 963 1554
Size: 12 411 128 191 221 591
Fragments arranged by size:
591 411 221 191 128 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 739 755 1554
Size: 739 16 799
Fragments arranged by size:
799 739 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 886 1357 1554
Size: 395 491 471 197
Fragments arranged by size:
491 471 395 197
Bpu10I CC'TnA_GC
Cuts at: 0 617 632 1554
Size: 617 15 922
Fragments arranged by size:
922 617 15
BsaAI yAC'GTr
Cuts at: 0 990 1554
Size: 990 564
BsaWI w'CCGG_w
Cuts at: 0 777 1554
Size: 777 777
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 883 913 1410 1440 1554
Size: 883 30 497 30 114
Fragments arranged by size:
883 497 114 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 474 1554
Size: 474 1080
BseYI C'CCAG_C
Cuts at: 0 279 1554
Size: 279 1275
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 373 841 1554
Size: 183 190 468 713
Fragments arranged by size:
713 468 190 183
BsiEI CG_ry'CG
Cuts at: 0 228 312 1554
Size: 228 84 1242
Fragments arranged by size:
1242 228 84
BsiHKAI G_wGCw'C
Cuts at: 0 815 1031 1187 1514 1554
Size: 815 216 156 327 40
Fragments arranged by size:
815 327 216 156 40
Bsp1286I G_dGCh'C
Cuts at: 0 12 423 458 484 551 584 742 815
Size: 12 411 35 26 67 33 158 73
Cuts at: 815 963 1031 1187 1298 1514 1554
Size: 148 68 156 111 216 40
Fragments arranged by size:
411 216 158 156 148 111 73 68
67 40 35 33 26 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 503 881 1554
Size: 503 378 673
Fragments arranged by size:
673 503 378
BsrBI CCG'CTC
Cuts at: 0 621 1554
Size: 621 933
BsrDI GCAATG_nn'
Cuts at: 0 1076 1554
Size: 1076 478
BstAPI GCAn_nnn'nTGC
Cuts at: 0 790 819 1554
Size: 790 29 735
Fragments arranged by size:
790 735 29
BstXI CCAn_nnnn'nTGG
Cuts at: 0 567 1554
Size: 567 987
BstYI r'GATC_y
Cuts at: 0 119 1451 1554
Size: 119 1332 103
Fragments arranged by size:
1332 119 103
Bsu36I CC'TnA_GG
Cuts at: 0 500 537 1554
Size: 500 37 1017
Fragments arranged by size:
1017 500 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1357 1554
Size: 1357 197
BtsI GCAGTG_nn'
Cuts at: 0 252 1554
Size: 252 1302
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 81 116 1058 1093 1128 1163 1554
Size: 81 35 942 35 35 35 391
Fragments arranged by size:
942 391 81 35 35 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 819 1554
Size: 819 735
EaeI y'GGCC_r
Cuts at: 0 309 569 1288 1554
Size: 309 260 719 266
Fragments arranged by size:
719 309 266 260
EagI C'GGCC_G
Cuts at: 0 309 1554
Size: 309 1245
EarI CTCTTCn'nnn_
Cuts at: 0 1519 1554
Size: 1519 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 154 1554
Size: 154 1400
EcoICRI GAG'CTC
Cuts at: 0 1029 1185 1512 1554
Size: 1029 156 327 42
Fragments arranged by size:
1029 327 156 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 886 1357 1500 1554
Size: 395 491 471 143 54
Fragments arranged by size:
491 471 395 143 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 413 499 973 1554
Size: 413 86 474 581
Fragments arranged by size:
581 474 413 86
EcoO109I rG'GnC_Cy
Cuts at: 0 8 9 419 548 590 738 870 1554
Size: 8 1 410 129 42 148 132 684
Fragments arranged by size:
684 410 148 132 129 42 8 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 177 1530 1554
Size: 177 1353 24
Fragments arranged by size:
1353 177 24
HaeII r_GCGC'y
Cuts at: 0 180 280 888 1554
Size: 180 100 608 666
Fragments arranged by size:
666 608 180 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 97 129 284 316 1554
Size: 97 32 155 32 1238
Fragments arranged by size:
1238 155 97 32 32
HindIII A'AGCT_T
Cuts at: 0 1411 1554
Size: 1411 143
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 914 1554
Size: 584 330 640
Fragments arranged by size:
640 584 330
MscI TGG'CCA
Cuts at: 0 571 1554
Size: 571 983
MspA1I CmG'CkG
Cuts at: 0 363 372 471 669 858 1323 1554
Size: 363 9 99 198 189 465 231
Fragments arranged by size:
465 363 231 198 189 99 9
NspI r_CATG'y
Cuts at: 0 288 318 346 854 1438 1554
Size: 288 30 28 508 584 116
Fragments arranged by size:
584 508 288 116 30 28
PasI CC'CwG_GG
Cuts at: 0 477 1554
Size: 477 1077
PflMI CCAn_nnn'nTGG
Cuts at: 0 1437 1554
Size: 1437 117
PpuMI rG'GwC_Cy
Cuts at: 0 870 1554
Size: 870 684
PspOMI G'GGCC_C
Cuts at: 0 8 419 547 738 1554
Size: 8 411 128 191 816
Fragments arranged by size:
816 411 191 128 8
PstI C_TGCA'G
Cuts at: 0 266 368 1180 1328 1451 1554
Size: 266 102 812 148 123 103
Fragments arranged by size:
812 266 148 123 103 102
PvuII CAG'CTG
Cuts at: 0 363 471 669 858 1323 1554
Size: 363 108 198 189 465 231
Fragments arranged by size:
465 363 231 198 189 108
SacI G_AGCT'C
Cuts at: 0 1031 1187 1514 1554
Size: 1031 156 327 40
Fragments arranged by size:
1031 327 156 40
SapI GCTCTTCn'nnn_
Cuts at: 0 1519 1554
Size: 1519 35
SexAI A'CCwGG_T
Cuts at: 0 815 1554
Size: 815 739
SfcI C'TryA_G
Cuts at: 0 262 364 651 840 1176 1324 1447 1554
Size: 262 102 287 189 336 148 123 107
Fragments arranged by size:
336 287 262 189 148 123 107 102
SmaI CCC'GGG
Cuts at: 0 904 1554
Size: 904 650
SphI G_CATG'C
Cuts at: 0 346 1554
Size: 346 1208
StyI C'CwwG_G
Cuts at: 0 423 449 572 1169 1403 1554
Size: 423 26 123 597 234 151
Fragments arranged by size:
597 423 234 151 123 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 695 1416 1554
Size: 695 721 138
Fragments arranged by size:
721 695 138
TatI w'GTAC_w
Cuts at: 0 1328 1554
Size: 1328 226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 987 1554
Size: 987 567
XmaI C'CCGG_G
Cuts at: 0 902 1554
Size: 902 652
Enzymes that do cut:
AcuI AfeI AhdI AlwNI ApaI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BglII Bme1580I BmrI BpmI
Bpu10I BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BstAPI BstXI BstYI
Bsu36I BtgZI BtsI CspCI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HindIII MmeI MscI MspA1I NspI PasI PflMI PpuMI
PspOMI PstI PvuII SacI SapI SexAI SfcI SmaI
SphI StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BciVI BclI BfrBI BglI BlpI
BmgBI BmtI BplI BpuEI BsaI BsaBI BsaHI BsiWI
BsmI BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I BtgI ClaI DraI DrdI EcoRI
EcoRV FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmnI ZraI