Restriction map for
pF1KE3552
(Linear) (Six-base) MAPSORT of: KIEE3552.seq Check: 2773 from: 1 to: 5874
KIEE3552.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AatII G_ACGT'C
Cuts at: 0 1317 1447 5874
Size: 1317 130 4427
Fragments arranged by size:
4427 1317 130
AccI GT'mk_AC
Cuts at: 0 4070 4806 5874
Size: 4070 736 1068
Fragments arranged by size:
4070 1068 736
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 914 2090 2525 2728 2769 3086 3663
Size: 134 780 1176 435 203 41 317 577
Cuts at: 3663 3862 4052 4520 4931 5027 5058 5874
Size: 199 190 468 411 96 31 816
Fragments arranged by size:
1176 816 780 577 468 435 411 317
203 199 190 134 96 41 31
AflII C'TTAA_G
Cuts at: 0 1036 3820 5874
Size: 1036 2784 2054
Fragments arranged by size:
2784 2054 1036
AflIII A'CryG_T
Cuts at: 0 10 5336 5874
Size: 10 5326 538
Fragments arranged by size:
5326 538 10
AgeI A'CCGG_T
Cuts at: 0 5507 5874
Size: 5507 367
AhdI GACnn_n'nnGTC
Cuts at: 0 2754 2827 5874
Size: 2754 73 3047
Fragments arranged by size:
3047 2754 73
AleI CACnn'nnGTG
Cuts at: 0 787 5874
Size: 787 5087
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1318 4237 5441 5874
Size: 1318 2919 1204 433
Fragments arranged by size:
2919 1318 1204 433
AlwNI CAG_nnn'CTG
Cuts at: 0 220 4630 5874
Size: 220 4410 1244
Fragments arranged by size:
4410 1244 220
ApoI r'AATT_y
Cuts at: 0 1028 1134 1643 1754 1791 3858 5001 5448
Size: 1028 106 509 111 37 2067 1143 447
Cuts at: 5448 5874
Size: 426
Fragments arranged by size:
2067 1143 1028 509 447 426 111 106
37
AseI AT'TA_AT
Cuts at: 0 2551 3487 5874
Size: 2551 936 2387
Fragments arranged by size:
2551 2387 936
AvaI C'yCGr_G
Cuts at: 0 1823 2373 5874
Size: 1823 550 3501
Fragments arranged by size:
3501 1823 550
AvrII C'CTAG_G
Cuts at: 0 1630 5874
Size: 1630 4244
BanII G_rGCy'C
Cuts at: 0 4582 4816 5325 5874
Size: 4582 234 509 549
Fragments arranged by size:
4582 549 509 234
BbsI GAAGACnn'nnnn_
Cuts at: 0 485 2753 2775 3074 3850 3938 4649 5874
Size: 485 2268 22 299 776 88 711 1225
Fragments arranged by size:
2268 1225 776 711 485 299 88 22
BciVI GTATCCnnnnn_n'
Cuts at: 0 291 5874
Size: 291 5583
BclI T'GATC_A
Cuts at: 0 2445 5874
Size: 2445 3429
BglI GCCn_nnn'nGGC
Cuts at: 0 2253 4672 5874
Size: 2253 2419 1202
Fragments arranged by size:
2419 2253 1202
BglII A'GATC_T
Cuts at: 0 1084 1764 5874
Size: 1084 680 4110
Fragments arranged by size:
4110 1084 680
BlpI GC'TnA_GC
Cuts at: 0 2609 4648 5526 5874
Size: 2609 2039 878 348
Fragments arranged by size:
2609 2039 878 348
Bme1580I G_kGCm'C
Cuts at: 0 1343 2016 5420 5874
Size: 1343 673 3404 454
Fragments arranged by size:
3404 1343 673 454
BmgBI CAC'GTC
Cuts at: 0 2332 3038 4655 5874
Size: 2332 706 1617 1219
Fragments arranged by size:
2332 1617 1219 706
BmrI ACTGGGnnnn_n'
Cuts at: 0 1338 2045 4402 5237 5874
Size: 1338 707 2357 835 637
Fragments arranged by size:
2357 1338 835 707 637
BmtI G_CTAG'C
Cuts at: 0 3088 5874
Size: 3088 2786
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 942 1122 4644 5874
Size: 942 180 3522 1230
Fragments arranged by size:
3522 1230 942 180
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 990 2078 3158 4785 5874
Size: 990 1088 1080 1627 1089
Fragments arranged by size:
1627 1089 1088 1080 990
Bpu10I CC'TnA_GC
Cuts at: 0 3692 5593 5874
Size: 3692 1901 281
Fragments arranged by size:
3692 1901 281
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 155 518 1059 1073 1415 3561 5874
Size: 34 121 363 541 14 342 2146 2313
Fragments arranged by size:
2313 2146 541 363 342 121 34 14
BsaI GGTCTCn'nnnn_
Cuts at: 0 69 2284 2998 5307 5874
Size: 69 2215 714 2309 567
Fragments arranged by size:
2309 2215 714 567 69
BsaAI yAC'GTr
Cuts at: 0 2697 5874
Size: 2697 3177
BsaBI GATnn'nnATC
Cuts at: 0 2637 2989 5874
Size: 2637 352 2885
Fragments arranged by size:
2885 2637 352
BsaHI Gr'CG_yC
Cuts at: 0 1314 1444 2232 3437 5874
Size: 1314 130 788 1205 2437
Fragments arranged by size:
2437 1314 1205 788 130
BsaWI w'CCGG_w
Cuts at: 0 5507 5874
Size: 5507 367
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 377 407 1665 1695 2281 2311 2508 2529
Size: 377 30 1258 30 586 30 197 21
Cuts at: 2529 2538 2559 5874
Size: 9 21 3315
Fragments arranged by size:
3315 1258 586 377 197 30 30 30
21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 278 653 1739 4381 5615 5874
Size: 278 375 1086 2642 1234 259
Fragments arranged by size:
2642 1234 1086 375 278 259
BseYI C'CCAG_C
Cuts at: 0 4076 4447 5874
Size: 4076 371 1427
Fragments arranged by size:
4076 1427 371
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4072 5874
Size: 4072 1802
BsiEI CG_ry'CG
Cuts at: 0 5657 5874
Size: 5657 217
BsiHKAI G_wGCw'C
Cuts at: 0 1440 4492 5874
Size: 1440 3052 1382
Fragments arranged by size:
3052 1440 1382
BsmI GAATG_Cn'
Cuts at: 0 3055 3377 5370 5817 5874
Size: 3055 322 1993 447 57
Fragments arranged by size:
3055 1993 447 322 57
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1451 5874
Size: 1451 4423
Bsp1286I G_dGCh'C
Cuts at: 0 1343 1440 2016 4492 4582 4816 5325 5420
Size: 1343 97 576 2476 90 234 509 95
Cuts at: 5420 5874
Size: 454
Fragments arranged by size:
2476 1343 576 509 454 234 97 95
90
BspHI T'CATG_A
Cuts at: 0 1329 2448 3389 5874
Size: 1329 1119 941 2485
Fragments arranged by size:
2485 1329 1119 941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 342 2317 5874
Size: 342 1975 3557
Fragments arranged by size:
3557 1975 342
BsrBI CCG'CTC
Cuts at: 0 5474 5874
Size: 5474 400
BsrDI GCAATG_nn'
Cuts at: 0 579 807 1774 4415 5676 5874
Size: 579 228 967 2641 1261 198
Fragments arranged by size:
2641 1261 967 579 228 198
BsrFI r'CCGG_y
Cuts at: 0 5507 5874
Size: 5507 367
BsrGI T'GTAC_A
Cuts at: 0 355 2915 5874
Size: 355 2560 2959
Fragments arranged by size:
2959 2560 355
BssSI C'ACGA_G
Cuts at: 0 5639 5874
Size: 5639 235
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3252 5812 5874
Size: 3252 2560 62
Fragments arranged by size:
3252 2560 62
BstBI TT'CG_AA
Cuts at: 0 3921 5874
Size: 3921 1953
BstEII G'GTnAC_C
Cuts at: 0 361 1844 3951 5874
Size: 361 1483 2107 1923
Fragments arranged by size:
2107 1923 1483 361
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1014 5706 5874
Size: 1014 4692 168
Fragments arranged by size:
4692 1014 168
BstYI r'GATC_y
Cuts at: 0 1084 1732 1764 3153 3750 4192 5613 5874
Size: 1084 648 32 1389 597 442 1421 261
Fragments arranged by size:
1421 1389 1084 648 597 442 261 32
Bsu36I CC'TnA_GG
Cuts at: 0 2209 4817 5874
Size: 2209 2608 1057
Fragments arranged by size:
2608 2209 1057
BtgI C'CryG_G
Cuts at: 0 638 2869 4829 5874
Size: 638 2231 1960 1045
Fragments arranged by size:
2231 1960 1045 638
BtsI GCAGTG_nn'
Cuts at: 0 794 1374 1816 5874
Size: 794 580 442 4058
Fragments arranged by size:
4058 794 580 442
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1566 1601 5874
Size: 1566 35 4273
Fragments arranged by size:
4273 1566 35
DraI TTT'AAA
Cuts at: 0 932 1749 5874
Size: 932 817 4125
Fragments arranged by size:
4125 932 817
DraIII CAC_nnn'GTG
Cuts at: 0 5361 5874
Size: 5361 513
DrdI GACnn_nn'nnGTC
Cuts at: 0 24 2943 3139 5874
Size: 24 2919 196 2735
Fragments arranged by size:
2919 2735 196 24
EaeI y'GGCC_r
Cuts at: 0 5 133 2866 4730 5558 5874
Size: 5 128 2733 1864 828 316
Fragments arranged by size:
2733 1864 828 316 128 5
EarI CTCTTCn'nnn_
Cuts at: 0 2948 3122 3299 3706 4028 4613 5077 5678
Size: 2948 174 177 407 322 585 464 601
Cuts at: 5678 5874
Size: 196
Fragments arranged by size:
2948 601 585 464 407 322 196 177
174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2375 5874
Size: 2375 3499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 914 990 2078 2090 2525 2728 2769
Size: 134 780 76 1088 12 435 203 41
Cuts at: 2769 3086 3158 3663 3862 4052 4520 4785 4931
Size: 317 72 505 199 190 468 265 146
Cuts at: 4931 5027 5058 5874
Size: 96 31 816
Fragments arranged by size:
1088 816 780 505 468 435 317 265
203 199 190 146 134 96 76 72
41 31 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3058 5874
Size: 3058 2816
EcoO109I rG'GnC_Cy
Cuts at: 0 139 331 725 877 2502 5535 5723 5874
Size: 139 192 394 152 1625 3033 188 151
Fragments arranged by size:
3033 1625 394 192 188 152 151 139
EcoRV GAT'ATC
Cuts at: 0 4472 5874
Size: 4472 1402
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2827 4057 5874
Size: 2827 1230 1817
Fragments arranged by size:
2827 1817 1230
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 838 870 1057 1089 2281 2313 2367 2399
Size: 838 32 187 32 1192 32 54 32
Cuts at: 2399 2566 2598 2740 2758 2772 2790 2813 2845
Size: 167 32 142 18 14 18 23 32
Cuts at: 2845 4810 4842 5470 5502 5591 5623 5874
Size: 1965 32 628 32 89 32 251
Fragments arranged by size:
1965 1192 838 628 251 187 167 142
89 54 32 32 32 32 32 32
32 32 32 23 18 18 14
HincII GTy'rAC
Cuts at: 0 3495 4807 5189 5225 5874
Size: 3495 1312 382 36 649
Fragments arranged by size:
3495 1312 649 382 36
HindIII A'AGCT_T
Cuts at: 0 560 5772 5874
Size: 560 5212 102
Fragments arranged by size:
5212 560 102
HpaI GTT'AAC
Cuts at: 0 5189 5874
Size: 5189 685
MluI A'CGCG_T
Cuts at: 0 10 5874
Size: 10 5864
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1195 2844 3065 3290 3465 5874
Size: 1195 1649 221 225 175 2409
Fragments arranged by size:
2409 1649 1195 225 221 175
MscI TGG'CCA
Cuts at: 0 7 135 4732 5560 5874
Size: 7 128 4597 828 314
Fragments arranged by size:
4597 828 314 128 7
MslI CAynn'nnrTG
Cuts at: 0 787 1064 3809 3958 5423 5874
Size: 787 277 2745 149 1465 451
Fragments arranged by size:
2745 1465 787 451 277 149
MspA1I CmG'CkG
Cuts at: 0 4246 4553 5500 5874
Size: 4246 307 947 374
Fragments arranged by size:
4246 947 374 307
NcoI C'CATG_G
Cuts at: 0 638 2869 5874
Size: 638 2231 3005
Fragments arranged by size:
3005 2231 638
NdeI CA'TA_TG
Cuts at: 0 2643 3499 5874
Size: 2643 856 2375
Fragments arranged by size:
2643 2375 856
NheI G'CTAG_C
Cuts at: 0 3084 5874
Size: 3084 2790
NspI r_CATG'y
Cuts at: 0 1897 3963 5340 5874
Size: 1897 2066 1377 534
Fragments arranged by size:
2066 1897 1377 534
PciI A'CATG_T
Cuts at: 0 5336 5874
Size: 5336 538
PfoI T'CCnGG_A
Cuts at: 0 3104 3555 5110 5874
Size: 3104 451 1555 764
Fragments arranged by size:
3104 1555 764 451
PpuMI rG'GwC_Cy
Cuts at: 0 139 331 877 2502 5535 5874
Size: 139 192 546 1625 3033 339
Fragments arranged by size:
3033 1625 546 339 192 139
PshAI GACnn'nnGTC
Cuts at: 0 4126 4802 5874
Size: 4126 676 1072
Fragments arranged by size:
4126 1072 676
PsiI TTA'TAA
Cuts at: 0 373 1274 5874
Size: 373 901 4600
Fragments arranged by size:
4600 901 373
PspXI vC'TCGA_Gb
Cuts at: 0 2373 5874
Size: 2373 3501
PstI C_TGCA'G
Cuts at: 0 1002 5808 5874
Size: 1002 4806 66
Fragments arranged by size:
4806 1002 66
PvuII CAG'CTG
Cuts at: 0 4246 4553 5500 5874
Size: 4246 307 947 374
Fragments arranged by size:
4246 947 374 307
SalI G'TCGA_C
Cuts at: 0 4805 5874
Size: 4805 1069
SapI GCTCTTCn'nnn_
Cuts at: 0 2948 4028 5077 5874
Size: 2948 1080 1049 797
Fragments arranged by size:
2948 1080 1049 797
SbfI CC_TGCA'GG
Cuts at: 0 1002 5874
Size: 1002 4872
ScaI AGT'ACT
Cuts at: 0 707 5874
Size: 707 5167
SexAI A'CCwGG_T
Cuts at: 0 717 1277 5874
Size: 717 560 4597
Fragments arranged by size:
4597 717 560
SfcI C'TryA_G
Cuts at: 0 496 729 998 3422 5804 5874
Size: 496 233 269 2424 2382 70
Fragments arranged by size:
2424 2382 496 269 233 70
SmlI C'TyrA_G
Cuts at: 0 49 170 533 1036 1074 1088 1430 2373
Size: 49 121 363 503 38 14 342 943
Cuts at: 2373 3576 3820 5874
Size: 1203 244 2054
Fragments arranged by size:
2054 1203 943 503 363 342 244 121
49 38 14
SpeI A'CTAG_T
Cuts at: 0 4159 5874
Size: 4159 1715
StuI AGG'CCT
Cuts at: 0 55 4666 5874
Size: 55 4611 1208
Fragments arranged by size:
4611 1208 55
StyI C'CwwG_G
Cuts at: 0 638 1630 2798 2869 5705 5874
Size: 638 992 1168 71 2836 169
Fragments arranged by size:
2836 1168 992 638 169 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 380 3970 5259 5671 5874
Size: 380 3590 1289 412 203
Fragments arranged by size:
3590 1289 412 380 203
TatI w'GTAC_w
Cuts at: 0 355 697 705 2915 3971 5874
Size: 355 342 8 2210 1056 1903
Fragments arranged by size:
2210 1903 1056 355 342 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1995 2027 3809 3841 5874
Size: 1995 32 1782 32 2033
Fragments arranged by size:
2033 1995 1782 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1313 2295 2943 5874
Size: 1313 982 648 2931
Fragments arranged by size:
2931 1313 982 648
XbaI T'CTAG_A
Cuts at: 0 3756 5874
Size: 3756 2118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1674 2584 4590 5874
Size: 1674 910 2006 1284
Fragments arranged by size:
2006 1674 1284 910
XhoI C'TCGA_G
Cuts at: 0 2373 5874
Size: 2373 3501
XmnI GAAnn'nnTTC
Cuts at: 0 482 1479 1869 2766 5874
Size: 482 997 390 897 3108
Fragments arranged by size:
3108 997 897 482 390
ZraI GAC'GTC
Cuts at: 0 1315 1445 5874
Size: 1315 130 4429
Fragments arranged by size:
4429 1315 130
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AgeI AhdI AleI
AlfI AlwNI ApoI AseI AvaI AvrII BanII BbsI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtsI CspCI DraI DraIII DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I HincII
HindIII HpaI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NspI PciI PfoI PpuMI PshAI PsiI
PspXI PstI PvuII SalI SapI SbfI ScaI SexAI
SfcI SmlI SpeI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AloI ApaI ApaLI AscI
AsiSI BaeI BamHI BanI BbeI BbvCI BcgI BfrBI
BsiWI BspEI BssHII BstZ17I BtgZI ClaI EagI EcoICRI
EcoRI FseI FspI FspAI HaeII KasI KpnI MfeI
NaeI NarI NgoMIV NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PspOMI PsrI PvuI RsrII
SacI SacII SanDI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI SspI SwaI XmaI