Restriction map for
pF1KE3547
(Linear) (Six-base) MAPSORT of: KIEE3547.seq Check: 1835 from: 1 to: 6189
KIEE3547.orf
With 182 enzymes: SgfI *
July 9, 2014 09:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1519 1651 3726 6189
Size: 1519 132 2075 2463
Fragments arranged by size:
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AatII G_ACGT'C
Cuts at: 0 3017 4763 5696 6189
Size: 3017 1746 933 493
Fragments arranged by size:
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AccI GT'mk_AC
Cuts at: 0 1368 5195 5384 5690 6189
Size: 1368 3827 189 306 499
Fragments arranged by size:
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Acc65I G'GTAC_C
Cuts at: 0 1987 6189
Size: 1987 4202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1711 2126 2261 3307 3691 3979 4474 6189
Size: 1711 415 135 1046 384 288 495 1715
Fragments arranged by size:
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AfeI AGC'GCT
Cuts at: 0 61 5777 6189
Size: 61 5716 412
Fragments arranged by size:
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AflIII A'CryG_T
Cuts at: 0 2865 5252 5552 5645 6189
Size: 2865 2387 300 93 544
Fragments arranged by size:
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AgeI A'CCGG_T
Cuts at: 0 3914 6189
Size: 3914 2275
AhdI GACnn_n'nnGTC
Cuts at: 0 1212 2131 2342 2736 4923 6146 6189
Size: 1212 919 211 394 2187 1223 43
Fragments arranged by size:
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AleI CACnn'nnGTG
Cuts at: 0 3047 6189
Size: 3047 3142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1992 3280 4504 5771 6189
Size: 1992 1288 1224 1267 418
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3028 3060 6189
Size: 3028 32 3129
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 1726 1915 1954 2149 2354 3285 3603 4209
Size: 1726 189 39 195 205 931 318 606
Cuts at: 4209 4272 6189
Size: 63 1917
Fragments arranged by size:
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63 39
ApaI G_GGCC'C
Cuts at: 0 194 352 681 772 2079 2533 2664 2850
Size: 194 158 329 91 1307 454 131 186
Cuts at: 2850 6189
Size: 3339
Fragments arranged by size:
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ApaLI G'TGCA_C
Cuts at: 0 5707 6189
Size: 5707 482
AvaI C'yCGr_G
Cuts at: 0 76 149 302 485 680 822 877 1880
Size: 76 73 153 183 195 142 55 1003
Cuts at: 1880 2742 2843 2975 3007 3352 4327 4669 4842
Size: 862 101 132 32 345 975 342 173
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Size: 99 66 158 833 191
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BamHI G'GATC_C
Cuts at: 0 1740 3635 6189
Size: 1740 1895 2554
Fragments arranged by size:
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BanI G'GyrC_C
Cuts at: 0 12 463 1321 1729 1816 1987 2110 2422
Size: 12 451 858 408 87 171 123 312
Cuts at: 2422 2629 2910 2997 4333 4665 4731 4896 5110
Size: 207 281 87 1336 332 66 165 214
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Size: 333 366 62 167 151
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BanII G_rGCy'C
Cuts at: 0 194 352 681 772 792 884 979 986
Size: 194 158 329 91 20 92 95 7
Cuts at: 986 1005 1280 1480 1712 1907 2079 2284 2293
Size: 19 275 200 232 195 172 205 9
Cuts at: 2293 2306 2533 2546 2564 2591 2664 2850 2982
Size: 13 227 13 18 27 73 186 132
Cuts at: 2982 3359 3438 4535 4742 5136 5218 5458 5530
Size: 377 79 1097 207 394 82 240 72
Cuts at: 5530 6189
Size: 659
Fragments arranged by size:
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BbsI GAAGACnn'nnnn_
Cuts at: 0 4073 4919 6189
Size: 4073 846 1270
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 82 1453 2216 2316 3113 4711 6189
Size: 82 1371 763 100 797 1598 1478
Fragments arranged by size:
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BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 378 412 2434 2468 4753 4787 6189
Size: 378 34 2022 34 2285 34 1402
Fragments arranged by size:
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BclI T'GATC_A
Cuts at: 0 3617 6189
Size: 3617 2572
BglI GCCn_nnn'nGGC
Cuts at: 0 165 1154 3538 3778 4746 6189
Size: 165 989 2384 240 968 1443
Fragments arranged by size:
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BglII A'GATC_T
Cuts at: 0 3335 6189
Size: 3335 2854
BlpI GC'TnA_GC
Cuts at: 0 3743 4591 6189
Size: 3743 848 1598
Fragments arranged by size:
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Bme1580I G_kGCm'C
Cuts at: 0 15 194 352 681 772 1821 2079 2115
Size: 15 179 158 329 91 1049 258 36
Cuts at: 2115 2533 2649 2664 2850 2888 4336 4670 4901
Size: 418 116 15 186 38 1448 334 231
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Size: 332 213 197 68 101 62 315
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BmgBI CAC'GTC
Cuts at: 0 4614 5916 6189
Size: 4614 1302 273
Fragments arranged by size:
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BmrI ACTGGGnnnn_n'
Cuts at: 0 1872 2261 3058 6189
Size: 1872 389 797 3131
Fragments arranged by size:
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BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1923 3457 4736 6189
Size: 1923 1534 1279 1453
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 84 396 732 806 1337 2555 2844
Size: 76 8 312 336 74 531 1218 289
Cuts at: 2844 3382 3403 3733 3826 4327 4706 4968 5130
Size: 538 21 330 93 501 379 262 162
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Size: 157 279 23 448 152
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Bpu10I CC'TnA_GC
Cuts at: 0 82 883 980 1453 1955 2216 2316 2915
Size: 82 801 97 473 502 261 100 599
Cuts at: 2915 3113 4711 5524 6189
Size: 198 1598 813 665
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BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 1180 1585 3541 5197 6189
Size: 132 1048 405 1956 1656 992
Fragments arranged by size:
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BsaI GGTCTCn'nnnn_
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Size: 568 615 219 684 1837 1073 30 1163
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BsaAI yAC'GTr
Cuts at: 0 3207 4572 5253 5562 5646 6189
Size: 3207 1365 681 309 84 543
Fragments arranged by size:
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BsaHI Gr'CG_yC
Cuts at: 0 184 314 508 3014 4370 4760 4858 5693
Size: 184 130 194 2506 1356 390 98 835
Cuts at: 5693 6189
Size: 496
Fragments arranged by size:
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BsaWI w'CCGG_w
Cuts at: 0 3046 3914 4734 6189
Size: 3046 868 820 1455
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BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 274 304 3028 3058 5200 5230 5248 5278
Size: 274 30 2724 30 2142 30 18 30
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Size: 85 30 146 30 216 30 198 30
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Size: 146
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BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 374 684 687 690 769 778 810
Size: 103 271 310 3 3 79 9 32
Cuts at: 810 1115 1692 1750 1915 2847 3790 4691 4728
Size: 305 577 58 165 932 943 901 37
Cuts at: 4728 4971 5014 5062 5065 5690 5806 6163 6189
Size: 243 43 48 3 625 116 357 26
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BseYI C'CCAG_C
Cuts at: 0 23 295 351 370 421 451 863 1235
Size: 23 272 56 19 51 30 412 372
Cuts at: 1235 1267 1566 1572 2491 2512 2775 3419 4933
Size: 32 299 6 919 21 263 644 1514
Cuts at: 4933 5226 6189
Size: 293 963
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BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1795 3025 3083 3649 3933 6189
Size: 1795 1230 58 566 284 2256
Fragments arranged by size:
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BsiEI CG_ry'CG
Cuts at: 0 136 329 344 598 840 3899 6189
Size: 136 193 15 254 242 3059 2290
Fragments arranged by size:
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BsiHKAI G_wGCw'C
Cuts at: 0 1480 1689 1712 1907 3311 3359 3368 5218
Size: 1480 209 23 195 1404 48 9 1850
Cuts at: 5218 5552 5711 6023 6189
Size: 334 159 312 166
Fragments arranged by size:
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159 48 23 9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 45 299 836 2801 4720 6146 6189
Size: 45 254 537 1965 1919 1426 43
Fragments arranged by size:
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Bsp1286I G_dGCh'C
Cuts at: 0 15 194 352 681 772 792 884 979
Size: 15 179 158 329 91 20 92 95
Cuts at: 979 986 1005 1280 1480 1689 1712 1821 1907
Size: 7 19 275 200 209 23 109 86
Cuts at: 1907 2079 2115 2284 2293 2306 2533 2546 2564
Size: 172 36 169 9 13 227 13 18
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Size: 27 58 15 186 38 94 329 48
Cuts at: 3359 3368 3438 4336 4535 4670 4742 4901 5136
Size: 9 70 898 199 135 72 159 235
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Size: 82 15 213 12 72 22 91 68
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Size: 101 62 149 166
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BspHI T'CATG_A
Cuts at: 0 3614 4469 6189
Size: 3614 855 1720
Fragments arranged by size:
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BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1519 1651 3047 3378 3726 5192 5332 5966
Size: 1519 132 1396 331 348 1466 140 634
Cuts at: 5966 6189
Size: 223
Fragments arranged by size:
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132
BsrBI CCG'CTC
Cuts at: 0 68 222 615 1250 5144 6189
Size: 68 154 393 635 3894 1045
Fragments arranged by size:
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BsrDI GCAATG_nn'
Cuts at: 0 2175 4832 5001 6189
Size: 2175 2657 169 1188
Fragments arranged by size:
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BsrFI r'CCGG_y
Cuts at: 0 131 593 3914 4967 5908 6189
Size: 131 462 3321 1053 941 281
Fragments arranged by size:
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BsrGI T'GTAC_A
Cuts at: 0 4001 6189
Size: 4001 2188
BssHII G'CGCG_C
Cuts at: 0 4599 6189
Size: 4599 1590
BssSI C'ACGA_G
Cuts at: 0 126 3370 6189
Size: 126 3244 2819
Fragments arranged by size:
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BstAPI GCAn_nnn'nTGC
Cuts at: 0 3030 3173 3402 3517 3576 4652 5715 6189
Size: 3030 143 229 115 59 1076 1063 474
Fragments arranged by size:
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BstEII G'GTnAC_C
Cuts at: 0 4876 6189
Size: 4876 1313
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2047 5121 6189
Size: 2047 3074 1068
Fragments arranged by size:
3074 2047 1068
BstYI r'GATC_y
Cuts at: 0 1740 1802 3335 3347 3635 3837 6189
Size: 1740 62 1533 12 288 202 2352
Fragments arranged by size:
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Bsu36I CC'TnA_GG
Cuts at: 0 2669 3128 3683 4541 5201 6189
Size: 2669 459 555 858 660 988
Fragments arranged by size:
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BtgI C'CryG_G
Cuts at: 0 140 323 329 807 1161 1488 1658 2040
Size: 140 183 6 478 354 327 170 382
Cuts at: 2040 2321 2499 5024 5151 5866 6189
Size: 281 178 2525 127 715 323
Fragments arranged by size:
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BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 690 1853 2987 4611 6189
Size: 690 1163 1134 1624 1578
Fragments arranged by size:
1624 1578 1163 1134 690
BtsI GCAGTG_nn'
Cuts at: 0 834 2373 2690 3396 4219 4488 6123 6141
Size: 834 1539 317 706 823 269 1635 18
Cuts at: 6141 6189
Size: 48
Fragments arranged by size:
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18
ClaI AT'CG_AT
Cuts at: 0 3494 6189
Size: 3494 2695
DraIII CAC_nnn'GTG
Cuts at: 0 2110 6189
Size: 2110 4079
DrdI GACnn_nn'nnGTC
Cuts at: 0 313 3943 6189
Size: 313 3630 2246
Fragments arranged by size:
3630 2246 313
EaeI y'GGCC_r
Cuts at: 0 133 197 326 341 595 813 855 1115
Size: 133 64 129 15 254 218 42 260
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Size: 27 2067 321 1833 532 15 279
Fragments arranged by size:
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133 129 64 42 27 15 15
EagI C'GGCC_G
Cuts at: 0 133 326 341 595 6189
Size: 133 193 15 254 5594
Fragments arranged by size:
5594 254 193 133 15
EarI CTCTTCn'nnn_
Cuts at: 0 400 755 2237 2366 3928 6189
Size: 400 355 1482 129 1562 2261
Fragments arranged by size:
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EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1034 2107 2743 3796 4629 6189
Size: 19 1015 1073 636 1053 833 1560
Fragments arranged by size:
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EcoICRI GAG'CTC
Cuts at: 0 1478 1710 1905 3357 5216 6189
Size: 1478 232 195 1452 1859 973
Fragments arranged by size:
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Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 84 396 732 806 1337 1711 2126
Size: 76 8 312 336 74 531 374 415
Cuts at: 2126 2261 2555 2844 3307 3382 3403 3691 3733
Size: 135 294 289 463 75 21 288 42
Cuts at: 3733 3826 3979 4327 4474 4706 4968 5130 5287
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42 23 21 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2668 3265 3385 3712 3868 4141 4870 5896
Size: 2668 597 120 327 156 273 729 1026
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Size: 293
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120
EcoO109I rG'GnC_Cy
Cuts at: 0 191 299 348 677 768 1060 1312 1723
Size: 191 108 49 329 91 292 252 411
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Size: 189 164 119 221 113 1 130 1
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Size: 583 51 143
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252 231 221 191 189 183 164 143
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24 1 1
EcoRV GAT'ATC
Cuts at: 0 1398 2386 6189
Size: 1398 988 3803
Fragments arranged by size:
3803 1398 988
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1143 6189
Size: 1143 5046
FspI TGC'GCA
Cuts at: 0 388 3256 6189
Size: 388 2868 2933
Fragments arranged by size:
2933 2868 388
HaeII r_GCGC'y
Cuts at: 0 63 264 428 2223 2775 3604 3789 4273
Size: 63 201 164 1795 552 829 185 484
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Size: 1506 410
Fragments arranged by size:
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164 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 274 306 421 453 1198 1230 1387 1419
Size: 274 32 115 32 745 32 157 32
Cuts at: 1419 1507 1539 1602 1634 2117 2149 2328 2360
Size: 88 32 63 32 483 32 179 32
Cuts at: 2360 2722 2754 3801 3833 4897 4909 4929 4941
Size: 362 32 1047 32 1064 12 20 12
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179 157 115 88 63 32 32 32
32 32 32 32 32 32 32 32
32 25 20 12 12
HincII GTy'rAC
Cuts at: 0 1369 5196 6189
Size: 1369 3827 993
Fragments arranged by size:
3827 1369 993
KpnI G_GTAC'C
Cuts at: 0 1991 6189
Size: 1991 4198
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 4238 5291 6132 6189
Size: 4238 1053 841 57
Fragments arranged by size:
4238 1053 841 57
MscI TGG'CCA
Cuts at: 0 1117 1144 3211 3532 5365 6189
Size: 1117 27 2067 321 1833 824
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MslI CAynn'nnrTG
Cuts at: 0 382 1996 2879 2896 3047 3262 3794 3886
Size: 382 1614 883 17 151 215 532 92
Cuts at: 3886 5467 6189
Size: 1581 722
Fragments arranged by size:
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92 17
MspA1I CmG'CkG
Cuts at: 0 295 325 331 631 780 863 1163 1374
Size: 295 30 6 300 149 83 300 211
Cuts at: 1374 1746 1890 2607 3285 3633 3723 4062 4237
Size: 372 144 717 678 348 90 339 175
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NaeI GCC'GGC
Cuts at: 0 133 595 6189
Size: 133 462 5594
Fragments arranged by size:
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NcoI C'CATG_G
Cuts at: 0 1658 2040 2321 6189
Size: 1658 382 281 3868
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NgoMIV G'CCGG_C
Cuts at: 0 131 593 6189
Size: 131 462 5596
Fragments arranged by size:
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NotI GC'GGCC_GC
Cuts at: 0 326 6189
Size: 326 5863
NspI r_CATG'y
Cuts at: 0 527 3033 3081 3198 3279 3405 3418 3579
Size: 527 2506 48 117 81 126 13 161
Cuts at: 3579 4491 5311 5556 6189
Size: 912 820 245 633
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117 81 48 13
PasI CC'CwG_GG
Cuts at: 0 2047 2427 4158 5448 6189
Size: 2047 380 1731 1290 741
Fragments arranged by size:
2047 1731 1290 741 380
PciI A'CATG_T
Cuts at: 0 5552 6189
Size: 5552 637
PflMI CCAn_nnn'nTGG
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Size: 634 1178 2397 871 45 1064
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PfoI T'CCnGG_A
Cuts at: 0 412 1022 1760 3359 3380 6189
Size: 412 610 738 1599 21 2809
Fragments arranged by size:
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PmlI CAC'GTG
Cuts at: 0 3207 5253 5646 6189
Size: 3207 2046 393 543
Fragments arranged by size:
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PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3028 3060 6189
Size: 3028 32 3129
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PpuMI rG'GwC_Cy
Cuts at: 0 299 1060 1312 2195 3054 3663 4146 4227
Size: 299 761 252 883 859 609 483 81
Cuts at: 4227 4251 5181 5412 6189
Size: 24 930 231 777
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252 231 81 24
PspOMI G'GGCC_C
Cuts at: 0 190 348 677 768 2075 2529 2660 2846
Size: 190 158 329 91 1307 454 131 186
Cuts at: 2846 6189
Size: 3343
Fragments arranged by size:
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91
PspXI vC'TCGA_Gb
Cuts at: 0 3007 6189
Size: 3007 3182
PstI C_TGCA'G
Cuts at: 0 919 3147 3178 3806 4679 4776 4955 5543
Size: 919 2228 31 628 873 97 179 588
Cuts at: 5543 5934 6189
Size: 391 255
Fragments arranged by size:
2228 919 873 628 588 391 255 179
97 31
PvuI CG_AT'CG
Cuts at: 0 3899 6189
Size: 3899 2290
PvuII CAG'CTG
Cuts at: 0 780 4237 4523 5544 5679 5861 5940 6189
Size: 780 3457 286 1021 135 182 79 249
Fragments arranged by size:
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SacI G_AGCT'C
Cuts at: 0 1480 1712 1907 3359 5218 6189
Size: 1480 232 195 1452 1859 971
Fragments arranged by size:
1859 1480 1452 971 232 195
SacII CC_GC'GG
Cuts at: 0 326 332 1164 6189
Size: 326 6 832 5025
Fragments arranged by size:
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SalI G'TCGA_C
Cuts at: 0 1367 5194 6189
Size: 1367 3827 995
Fragments arranged by size:
3827 1367 995
SanDI GG'GwC_CC
Cuts at: 0 299 5412 6189
Size: 299 5113 777
Fragments arranged by size:
5113 777 299
SapI GCTCTTCn'nnn_
Cuts at: 0 400 2237 6189
Size: 400 1837 3952
Fragments arranged by size:
3952 1837 400
SexAI A'CCwGG_T
Cuts at: 0 1467 2106 2625 5345 6189
Size: 1467 639 519 2720 844
Fragments arranged by size:
2720 1467 844 639 519
SfcI C'TryA_G
Cuts at: 0 915 2190 3143 3174 3802 4675 4772 4951
Size: 915 1275 953 31 628 873 97 179
Cuts at: 4951 5539 5930 6189
Size: 588 391 259
Fragments arranged by size:
1275 953 915 873 628 588 391 259
179 97 31
SmaI CCC'GGG
Cuts at: 0 78 151 682 879 4671 5167 6189
Size: 78 73 531 197 3792 496 1022
Fragments arranged by size:
3792 1022 531 496 197 78 73
SmlI C'TyrA_G
Cuts at: 0 111 1195 1600 3007 3556 5176 6189
Size: 111 1084 405 1407 549 1620 1013
Fragments arranged by size:
1620 1407 1084 1013 549 405 111
SphI G_CATG'C
Cuts at: 0 3405 3418 3579 4491 5311 6189
Size: 3405 13 161 912 820 878
Fragments arranged by size:
3405 912 878 820 161 13
SrfI GCCC'GGGC
Cuts at: 0 682 879 4671 6189
Size: 682 197 3792 1518
Fragments arranged by size:
3792 1518 682 197
StuI AGG'CCT
Cuts at: 0 888 921 2188 3546 4393 4621 5206 5891
Size: 888 33 1267 1358 847 228 585 685
Cuts at: 5891 6063 6189
Size: 172 126
Fragments arranged by size:
1358 1267 888 847 685 585 228 172
126 33
StyI C'CwwG_G
Cuts at: 0 1118 1658 2040 2321 3458 3533 4188 4377
Size: 1118 540 382 281 1137 75 655 189
Cuts at: 4377 5015 5438 6034 6189
Size: 638 423 596 155
Fragments arranged by size:
1137 1118 655 638 596 540 423 382
281 189 155 75
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 592 854 1804 5461 5470 5602 5610 5793
Size: 592 262 950 3657 9 132 8 183
Cuts at: 5793 6189
Size: 396
Fragments arranged by size:
3657 950 592 396 262 183 132 9
8
TatI w'GTAC_w
Cuts at: 0 3026 4001 4037 6189
Size: 3026 975 36 2152
Fragments arranged by size:
3026 2152 975 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5363 5395 5785 5817 6013 6045 6189
Size: 5363 32 390 32 196 32 144
Fragments arranged by size:
5363 390 196 144 32 32 32
XbaI T'CTAG_A
Cuts at: 0 4437 6189
Size: 4437 1752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1828 2044 2319 3219 4413 5466 5944 6189
Size: 1828 216 275 900 1194 1053 478 245
Fragments arranged by size:
1828 1194 1053 900 478 275 245 216
XhoI C'TCGA_G
Cuts at: 0 3007 6189
Size: 3007 3182
XmaI C'CCGG_G
Cuts at: 0 76 149 680 877 4669 5165 6189
Size: 76 73 531 197 3792 496 1024
Fragments arranged by size:
3792 1024 531 496 197 76 73
ZraI GAC'GTC
Cuts at: 0 3015 4761 5694 6189
Size: 3015 1746 933 495
Fragments arranged by size:
3015 1746 933 495
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbsI BbvCI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI FspI HaeII Hin4I HincII KpnI MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NotI NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII SacI SacII SalI SanDI
SapI SexAI SfcI SmaI SmlI SphI SrfI StuI
StyI TaqII TatI TstI XbaI XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AclI AflII ApoI AscI AseI AsiSI AvrII BaeI
BbeI BciVI BfrBI BmtI BsaBI BsiWI BsmI BspEI
BstBI BstZ17I CspCI DraI EcoRI FseI FspAI HindIII
HpaI KasI MfeI MluI NarI NdeI NheI NruI
NsiI PacI PmeI PshAI PsiI PsrI RsrII SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SspI
SwaI Tth111I XmnI