Restriction map for
pF1KE3526
(Linear) (Six-base) MAPSORT of: KIEE3526.seq Check: 1349 from: 1 to: 3108
KIEE3526.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1803 3108
Size: 1803 1305
AatII G_ACGT'C
Cuts at: 0 154 1574 1934 3108
Size: 154 1420 360 1174
Fragments arranged by size:
1420 1174 360 154
Acc65I G'GTAC_C
Cuts at: 0 557 3108
Size: 557 2551
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 292 1822 2590 2986 3108
Size: 31 261 1530 768 396 122
Fragments arranged by size:
1530 768 396 261 122 31
AfeI AGC'GCT
Cuts at: 0 959 1396 3108
Size: 959 437 1712
Fragments arranged by size:
1712 959 437
AflIII A'CryG_T
Cuts at: 0 1346 3108
Size: 1346 1762
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1973 2372 3108
Size: 1973 399 736
Fragments arranged by size:
1973 736 399
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1684 1716 3108
Size: 1684 32 1392
Fragments arranged by size:
1684 1392 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1308 2179 2844 3108
Size: 1308 871 665 264
Fragments arranged by size:
1308 871 665 264
ApaI G_GGCC'C
Cuts at: 0 598 1927 2515 2599 2689 3108
Size: 598 1329 588 84 90 419
Fragments arranged by size:
1329 598 588 419 90 84
ApaLI G'TGCA_C
Cuts at: 0 106 766 2584 3108
Size: 106 660 1818 524
Fragments arranged by size:
1818 660 524 106
ApoI r'AATT_y
Cuts at: 0 1324 3108
Size: 1324 1784
AscI GG'CGCG_CC
Cuts at: 0 2072 3108
Size: 2072 1036
AvaI C'yCGr_G
Cuts at: 0 715 1420 1806 1988 3108
Size: 715 705 386 182 1120
Fragments arranged by size:
1120 715 705 386 182
AvrII C'CTAG_G
Cuts at: 0 939 3108
Size: 939 2169
BamHI G'GATC_C
Cuts at: 0 990 1559 3108
Size: 990 569 1549
Fragments arranged by size:
1549 990 569
BanI G'GyrC_C
Cuts at: 0 557 2059 2097 2340 3108
Size: 557 1502 38 243 768
Fragments arranged by size:
1502 768 557 243 38
BanII G_rGCy'C
Cuts at: 0 598 722 1478 1645 1927 2203 2515 2599
Size: 598 124 756 167 282 276 312 84
Cuts at: 2599 2614 2689 2732 3108
Size: 15 75 43 376
Fragments arranged by size:
756 598 376 312 282 276 167 124
84 75 43 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 1083 2678 3108
Size: 1083 1595 430
Fragments arranged by size:
1595 1083 430
BbvCI CC'TCA_GC
Cuts at: 0 73 3108
Size: 73 3035
BciVI GTATCCnnnnn_n'
Cuts at: 0 1545 3108
Size: 1545 1563
BfrBI ATG'CAT
Cuts at: 0 3105 3108
Size: 3105 3
BglI GCCn_nnn'nGGC
Cuts at: 0 529 886 2082 3108
Size: 529 357 1196 1026
Fragments arranged by size:
1196 1026 529 357
BglII A'GATC_T
Cuts at: 0 1409 3108
Size: 1409 1699
BlpI GC'TnA_GC
Cuts at: 0 40 191 674 1964 2446 2731 3108
Size: 40 151 483 1290 482 285 377
Fragments arranged by size:
1290 483 482 377 285 151 40
Bme1580I G_kGCm'C
Cuts at: 0 110 598 770 1927 2515 2588 2599 2689
Size: 110 488 172 1157 588 73 11 90
Cuts at: 2689 3081 3108
Size: 392 27
Fragments arranged by size:
1157 588 488 392 172 110 90 73
27 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 1552 1993 2510 3108
Size: 1552 441 517 598
Fragments arranged by size:
1552 598 517 441
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 39 1446 1929 3108
Size: 39 1407 483 1179
Fragments arranged by size:
1407 1179 483 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1426 1464 1540 1864 2815 2872 2890 3108
Size: 1426 38 76 324 951 57 18 218
Fragments arranged by size:
1426 951 324 218 76 57 38 18
Bpu10I CC'TnA_GC
Cuts at: 0 73 2197 2713 3108
Size: 73 2124 516 395
Fragments arranged by size:
2124 516 395 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 889 1024 2457 3108
Size: 358 531 135 1433 651
Fragments arranged by size:
1433 651 531 358 135
BsaI GGTCTCn'nnnn_
Cuts at: 0 454 1417 2800 3108
Size: 454 963 1383 308
Fragments arranged by size:
1383 963 454 308
BsaBI GATnn'nnATC
Cuts at: 0 613 3108
Size: 613 2495
BsaHI Gr'CG_yC
Cuts at: 0 151 571 935 1571 1886 1931 2431 2488
Size: 151 420 364 636 315 45 500 57
Cuts at: 2488 3108
Size: 620
Fragments arranged by size:
636 620 500 420 364 315 151 57
45
BsaWI w'CCGG_w
Cuts at: 0 1702 3108
Size: 1702 1406
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 541 571 1615 1645 3108
Size: 541 30 1044 30 1463
Fragments arranged by size:
1463 1044 541 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 760 910 2233 2281 2385 2812 2842 2887
Size: 760 150 1323 48 104 427 30 45
Cuts at: 2887 2896 3108
Size: 9 212
Fragments arranged by size:
1323 760 427 212 150 104 48 45
30 9
BseYI C'CCAG_C
Cuts at: 0 46 994 1209 1666 2087 2241 3086 3108
Size: 46 948 215 457 421 154 845 22
Fragments arranged by size:
948 845 457 421 215 154 46 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 728 1158 1471 3108
Size: 728 430 313 1637
Fragments arranged by size:
1637 728 430 313
BsiEI CG_ry'CG
Cuts at: 0 1010 1152 2427 3108
Size: 1010 142 1275 681
Fragments arranged by size:
1275 1010 681 142
BsiHKAI G_wGCw'C
Cuts at: 0 110 266 350 404 680 770 784 854
Size: 110 156 84 54 276 90 14 70
Cuts at: 854 1006 1028 1478 1591 2203 2452 2588 2732
Size: 152 22 450 113 612 249 136 144
Cuts at: 2732 3108
Size: 376
Fragments arranged by size:
612 450 376 276 249 156 152 144
136 113 110 90 84 70 54 22
14
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1736 2824 3108
Size: 1736 1088 284
Fragments arranged by size:
1736 1088 284
Bsp1286I G_dGCh'C
Cuts at: 0 110 266 350 404 598 680 722 770
Size: 110 156 84 54 194 82 42 48
Cuts at: 770 784 854 1006 1028 1478 1591 1645 1927
Size: 14 70 152 22 450 113 54 282
Cuts at: 1927 2203 2452 2515 2588 2599 2614 2689 2732
Size: 276 249 63 73 11 15 75 43
Cuts at: 2732 3081 3108
Size: 349 27
Fragments arranged by size:
450 349 282 276 249 194 156 152
113 110 84 82 75 73 70 63
54 54 48 43 42 27 22 15
14 11
BspHI T'CATG_A
Cuts at: 0 1355 3071 3108
Size: 1355 1716 37
Fragments arranged by size:
1716 1355 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 308 525 935 1247 1803 2450 2930 3108
Size: 308 217 410 312 556 647 480 178
Fragments arranged by size:
647 556 480 410 312 308 217 178
BsrBI CCG'CTC
Cuts at: 0 195 226 1170 2103 2886 3108
Size: 195 31 944 933 783 222
Fragments arranged by size:
944 933 783 222 195 31
BsrDI GCAATG_nn'
Cuts at: 0 3038 3108
Size: 3038 70
BsrFI r'CCGG_y
Cuts at: 0 179 251 1441 1983 2081 3108
Size: 179 72 1190 542 98 1027
Fragments arranged by size:
1190 1027 542 179 98 72
BsrGI T'GTAC_A
Cuts at: 0 659 3108
Size: 659 2449
BssHII G'CGCG_C
Cuts at: 0 622 2072 3108
Size: 622 1450 1036
Fragments arranged by size:
1450 1036 622
BssSI C'ACGA_G
Cuts at: 0 28 1122 3108
Size: 28 1094 1986
Fragments arranged by size:
1986 1094 28
BstAPI GCAn_nnn'nTGC
Cuts at: 0 774 1494 3108
Size: 774 720 1614
Fragments arranged by size:
1614 774 720
BstEII G'GTnAC_C
Cuts at: 0 1296 1724 3108
Size: 1296 428 1384
Fragments arranged by size:
1384 1296 428
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1626 2622 2793 3108
Size: 1626 996 171 315
Fragments arranged by size:
1626 996 315 171
BstYI r'GATC_y
Cuts at: 0 990 1409 1559 3108
Size: 990 419 150 1549
Fragments arranged by size:
1549 990 419 150
Bsu36I CC'TnA_GG
Cuts at: 0 1564 2527 3108
Size: 1564 963 581
Fragments arranged by size:
1564 963 581
BtgI C'CryG_G
Cuts at: 0 362 650 1096 1868 2021 2157 2261 2336
Size: 362 288 446 772 153 136 104 75
Cuts at: 2336 2344 2421 2575 2951 3108
Size: 8 77 154 376 157
Fragments arranged by size:
772 446 376 362 288 157 154 153
136 104 77 75 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1272 3108
Size: 1272 1836
BtsI GCAGTG_nn'
Cuts at: 0 57 1265 1492 2255 2588 3108
Size: 57 1208 227 763 333 520
Fragments arranged by size:
1208 763 520 333 227 57
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1085 1120 3108
Size: 1085 35 1988
Fragments arranged by size:
1988 1085 35
DraIII CAC_nnn'GTG
Cuts at: 0 2158 3108
Size: 2158 950
EaeI y'GGCC_r
Cuts at: 0 123 530 878 1007 1149 1310 1844 1973
Size: 123 407 348 129 142 161 534 129
Cuts at: 1973 1981 2018 2424 3108
Size: 8 37 406 684
Fragments arranged by size:
684 534 407 406 348 161 142 129
129 123 37 8
EagI C'GGCC_G
Cuts at: 0 1007 1149 2424 3108
Size: 1007 142 1275 684
Fragments arranged by size:
1275 1007 684 142
EarI CTCTTCn'nnn_
Cuts at: 0 80 1195 1237 2547 3108
Size: 80 1115 42 1310 561
Fragments arranged by size:
1310 1115 561 80 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2378 3108
Size: 2378 730
EcoICRI GAG'CTC
Cuts at: 0 1476 2201 2730 3108
Size: 1476 725 529 378
Fragments arranged by size:
1476 725 529 378
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 292 1426 1464 1540 1822 1864 2590
Size: 31 261 1134 38 76 282 42 726
Cuts at: 2590 2815 2872 2890 2986 3108
Size: 225 57 18 96 122
Fragments arranged by size:
1134 726 282 261 225 122 96 76
57 42 38 31 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 124 1125 1243 3108
Size: 124 1001 118 1865
Fragments arranged by size:
1865 1001 124 118
EcoO109I rG'GnC_Cy
Cuts at: 0 297 594 595 1546 1772 1924 2512 2595
Size: 297 297 1 951 226 152 588 83
Cuts at: 2595 2596 2679 2748 3108
Size: 1 83 69 360
Fragments arranged by size:
951 588 360 297 297 226 152 83
83 69 1 1
FseI GG_CCGG'CC
Cuts at: 0 1987 2085 3108
Size: 1987 98 1023
Fragments arranged by size:
1987 1023 98
FspI TGC'GCA
Cuts at: 0 433 3108
Size: 433 2675
HaeII r_GCGC'y
Cuts at: 0 67 961 1184 1398 3108
Size: 67 894 223 214 1710
Fragments arranged by size:
1710 894 223 214 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 593 625 739 771 1615 1647 2161 2193
Size: 593 32 114 32 844 32 514 32
Cuts at: 2193 3108
Size: 915
Fragments arranged by size:
915 844 593 514 114 32 32 32
32
KpnI G_GTAC'C
Cuts at: 0 561 3108
Size: 561 2547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1439 1759 3029 3108
Size: 1439 320 1270 79
Fragments arranged by size:
1439 1270 320 79
MscI TGG'CCA
Cuts at: 0 125 880 1312 1846 2020 3108
Size: 125 755 432 534 174 1088
Fragments arranged by size:
1088 755 534 432 174 125
MslI CAynn'nnrTG
Cuts at: 0 655 2920 3108
Size: 655 2265 188
Fragments arranged by size:
2265 655 188
MspA1I CmG'CkG
Cuts at: 0 190 836 1012 1518 1666 1957 1968 2091
Size: 190 646 176 506 148 291 11 123
Cuts at: 2091 2148 2263 2423 3090 3108
Size: 57 115 160 667 18
Fragments arranged by size:
667 646 506 291 190 176 160 148
123 115 57 18 11
NaeI GCC'GGC
Cuts at: 0 253 1443 1985 2083 3108
Size: 253 1190 542 98 1025
Fragments arranged by size:
1190 1025 542 253 98
NcoI C'CATG_G
Cuts at: 0 650 1868 3108
Size: 650 1218 1240
Fragments arranged by size:
1240 1218 650
NgoMIV G'CCGG_C
Cuts at: 0 251 1441 1983 2081 3108
Size: 251 1190 542 98 1027
Fragments arranged by size:
1190 1027 542 251 98
NotI GC'GGCC_GC
Cuts at: 0 1149 3108
Size: 1149 1959
NsiI A_TGCA'T
Cuts at: 0 3107 3108
Size: 3107 1
NspI r_CATG'y
Cuts at: 0 1350 1392 3108
Size: 1350 42 1716
Fragments arranged by size:
1716 1350 42
PasI CC'CwG_GG
Cuts at: 0 1612 3108
Size: 1612 1496
PciI A'CATG_T
Cuts at: 0 1346 3108
Size: 1346 1762
PflMI CCAn_nnn'nTGG
Cuts at: 0 696 1308 2094 3108
Size: 696 612 786 1014
Fragments arranged by size:
1014 786 696 612
PfoI T'CCnGG_A
Cuts at: 0 863 1880 3108
Size: 863 1017 1228
Fragments arranged by size:
1228 1017 863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2797 2829 3108
Size: 2797 32 279
Fragments arranged by size:
2797 279 32
PpuMI rG'GwC_Cy
Cuts at: 0 297 1546 1772 2748 3108
Size: 297 1249 226 976 360
Fragments arranged by size:
1249 976 360 297 226
PshAI GACnn'nnGTC
Cuts at: 0 2307 3108
Size: 2307 801
PspOMI G'GGCC_C
Cuts at: 0 594 1923 2511 2595 2685 3108
Size: 594 1329 588 84 90 423
Fragments arranged by size:
1329 594 588 423 90 84
PspXI vC'TCGA_Gb
Cuts at: 0 715 1806 3108
Size: 715 1091 1302
Fragments arranged by size:
1302 1091 715
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 652 684 2107 2139 3108
Size: 652 32 1423 32 969
Fragments arranged by size:
1423 969 652 32 32
PstI C_TGCA'G
Cuts at: 0 189 779 932 1247 1676 1962 2459 2765
Size: 189 590 153 315 429 286 497 306
Cuts at: 2765 3108
Size: 343
Fragments arranged by size:
590 497 429 343 315 306 286 189
153
PvuII CAG'CTG
Cuts at: 0 190 1518 1666 1968 2091 3090 3108
Size: 190 1328 148 302 123 999 18
Fragments arranged by size:
1328 999 302 190 148 123 18
SacI G_AGCT'C
Cuts at: 0 1478 2203 2732 3108
Size: 1478 725 529 376
Fragments arranged by size:
1478 725 529 376
SacII CC_GC'GG
Cuts at: 0 2264 2424 3108
Size: 2264 160 684
Fragments arranged by size:
2264 684 160
SanDI GG'GwC_CC
Cuts at: 0 1546 3108
Size: 1546 1562
SbfI CC_TGCA'GG
Cuts at: 0 932 3108
Size: 932 2176
SfcI C'TryA_G
Cuts at: 0 185 775 928 1243 1672 1958 2455 2761
Size: 185 590 153 315 429 286 497 306
Cuts at: 2761 3108
Size: 347
Fragments arranged by size:
590 497 429 347 315 306 286 185
153
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 886 3108
Size: 886 2222
SmaI CCC'GGG
Cuts at: 0 1990 3108
Size: 1990 1118
SmlI C'TyrA_G
Cuts at: 0 373 715 904 1039 1806 2472 3108
Size: 373 342 189 135 767 666 636
Fragments arranged by size:
767 666 636 373 342 189 135
SrfI GCCC'GGGC
Cuts at: 0 1990 3108
Size: 1990 1118
StyI C'CwwG_G
Cuts at: 0 120 293 581 650 740 884 939 1292
Size: 120 173 288 69 90 144 55 353
Cuts at: 1292 1868 2163 2549 3108
Size: 576 295 386 559
Fragments arranged by size:
576 559 386 353 295 288 173 144
120 90 69 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 270 478 1315 2496 3108
Size: 270 208 837 1181 612
Fragments arranged by size:
1181 837 612 270 208
TatI w'GTAC_w
Cuts at: 0 659 3108
Size: 659 2449
Tth111I GACn'n_nGTC
Cuts at: 0 150 3108
Size: 150 2958
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 54 648 693 747 2134 2790 3108
Size: 54 594 45 54 1387 656 318
Fragments arranged by size:
1387 656 594 318 54 54 45
XhoI C'TCGA_G
Cuts at: 0 715 1806 3108
Size: 715 1091 1302
Fragments arranged by size:
1302 1091 715
XmaI C'CCGG_G
Cuts at: 0 1988 3108
Size: 1988 1120
XmnI GAAnn'nnTTC
Cuts at: 0 799 1363 3108
Size: 799 564 1745
Fragments arranged by size:
1745 799 564
ZraI GAC'GTC
Cuts at: 0 152 1572 1932 3108
Size: 152 1420 360 1176
Fragments arranged by size:
1420 1176 360 152
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AlfI AloI
AlwNI ApaI ApaLI ApoI AscI AvaI AvrII BamHI
BanI BanII BbsI BbvCI BciVI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FseI FspI HaeII
Hin4I KpnI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NotI NsiI NspI PasI PciI PflMI PfoI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuII
SacI SacII SanDI SbfI SfcI SfiI SmaI SmlI
SrfI StyI TaqII TatI Tth111I XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI AhdI AleI AseI AsiSI
BaeI BbeI BcgI BclI BmgBI BmtI BsaAI BsiWI
BsmI BspEI BstBI BstZ17I ClaI DraI DrdI EcoRI
EcoRV FalI FspAI HincII HindIII HpaI KasI MfeI
MluI NarI NdeI NheI NruI PacI PmeI PmlI
PsiI PvuI RsrII SalI SapI ScaI SexAI SfoI
SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI
TstI XbaI