Restriction map for
pF1KE3380
(Linear) (Six-base) MAPSORT of: KIEE3380.seq Check: 4286 from: 1 to: 3396
KIEE3380.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
Acc65I G'GTAC_C
Cuts at: 0 2154 2218 3396
Size: 2154 64 1178
Fragments arranged by size:
2154 1178 64
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 440 821 1258 1395 1518 2119 2635
Size: 100 340 381 437 137 123 601 516
Cuts at: 2635 2684 3396
Size: 49 712
Fragments arranged by size:
712 601 516 437 381 340 137 123
100 49
AflII C'TTAA_G
Cuts at: 0 754 3396
Size: 754 2642
AflIII A'CryG_T
Cuts at: 0 2459 3396
Size: 2459 937
AleI CACnn'nnGTG
Cuts at: 0 235 2325 3396
Size: 235 2090 1071
Fragments arranged by size:
2090 1071 235
ApoI r'AATT_y
Cuts at: 0 805 884 1888 2184 2190 2548 3178 3396
Size: 805 79 1004 296 6 358 630 218
Fragments arranged by size:
1004 805 630 358 296 218 79 6
AseI AT'TA_AT
Cuts at: 0 1102 2779 3396
Size: 1102 1677 617
Fragments arranged by size:
1677 1102 617
AvaI C'yCGr_G
Cuts at: 0 410 3396
Size: 410 2986
AvrII C'CTAG_G
Cuts at: 0 2487 3396
Size: 2487 909
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 223 256 742 775 3396
Size: 223 33 486 33 2621
Fragments arranged by size:
2621 486 223 33 33
BamHI G'GATC_C
Cuts at: 0 2517 3396
Size: 2517 879
BanI G'GyrC_C
Cuts at: 0 2154 2218 2482 2500 3396
Size: 2154 64 264 18 896
Fragments arranged by size:
2154 896 264 64 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 294 1524 2060 2068 2516 3396
Size: 294 1230 536 8 448 880
Fragments arranged by size:
1230 880 536 448 294 8
BciVI GTATCCnnnnn_n'
Cuts at: 0 296 3396
Size: 296 3100
BclI T'GATC_A
Cuts at: 0 3299 3396
Size: 3299 97
BfrBI ATG'CAT
Cuts at: 0 1983 2168 3396
Size: 1983 185 1228
Fragments arranged by size:
1983 1228 185
BglII A'GATC_T
Cuts at: 0 1425 2409 3290 3396
Size: 1425 984 881 106
Fragments arranged by size:
1425 984 881 106
Bme1580I G_kGCm'C
Cuts at: 0 2487 3396
Size: 2487 909
BmrI ACTGGGnnnn_n'
Cuts at: 0 2633 3396
Size: 2633 763
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1604 3396
Size: 1604 1792
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1151 2414 3050 3396
Size: 1151 1263 636 346
Fragments arranged by size:
1263 1151 636 346
BsaBI GATnn'nnATC
Cuts at: 0 1136 1907 2306 3396
Size: 1136 771 399 1090
Fragments arranged by size:
1136 1090 771 399
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1832 1862 2202 2232 3396
Size: 1832 30 340 30 1164
Fragments arranged by size:
1832 1164 340 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 416 859 2261 3396
Size: 416 443 1402 1135
Fragments arranged by size:
1402 1135 443 416
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2303 3396
Size: 2303 1093
BsiHKAI G_wGCw'C
Cuts at: 0 2268 2672 3396
Size: 2268 404 724
Fragments arranged by size:
2268 724 404
BsiWI C'GTAC_G
Cuts at: 0 1686 3396
Size: 1686 1710
BsmI GAATG_Cn'
Cuts at: 0 2170 3316 3396
Size: 2170 1146 80
Fragments arranged by size:
2170 1146 80
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1850 3396
Size: 1850 1546
Bsp1286I G_dGCh'C
Cuts at: 0 2268 2487 2672 3396
Size: 2268 219 185 724
Fragments arranged by size:
2268 724 219 185
BspHI T'CATG_A
Cuts at: 0 513 3302 3396
Size: 513 2789 94
Fragments arranged by size:
2789 513 94
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1237 3396
Size: 1237 2159
BsrDI GCAATG_nn'
Cuts at: 0 3204 3396
Size: 3204 192
BsrFI r'CCGG_y
Cuts at: 0 2597 3396
Size: 2597 799
BstXI CCAn_nnnn'nTGG
Cuts at: 0 139 3396
Size: 139 3257
BstYI r'GATC_y
Cuts at: 0 105 278 650 1425 2409 2517 2925 3290
Size: 105 173 372 775 984 108 408 365
Cuts at: 3290 3351 3396
Size: 61 45
Fragments arranged by size:
984 775 408 372 365 173 108 105
61 45
Bsu36I CC'TnA_GG
Cuts at: 0 1286 3396
Size: 1286 2110
BtgI C'CryG_G
Cuts at: 0 2149 3396
Size: 2149 1247
BtsI GCAGTG_nn'
Cuts at: 0 381 551 734 2247 3396
Size: 381 170 183 1513 1149
Fragments arranged by size:
1513 1149 381 183 170
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1374 1409 3396
Size: 1374 35 1987
Fragments arranged by size:
1987 1374 35
DraI TTT'AAA
Cuts at: 0 1680 1739 1832 2189 3396
Size: 1680 59 93 357 1207
Fragments arranged by size:
1680 1207 357 93 59
DraIII CAC_nnn'GTG
Cuts at: 0 2228 3396
Size: 2228 1168
EaeI y'GGCC_r
Cuts at: 0 593 1653 3396
Size: 593 1060 1743
Fragments arranged by size:
1743 1060 593
EarI CTCTTCn'nnn_
Cuts at: 0 54 434 1373 1686 2528 2660 3151 3365
Size: 54 380 939 313 842 132 491 214
Cuts at: 3365 3396
Size: 31
Fragments arranged by size:
939 842 491 380 313 214 132 54
31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 440 821 1151 1258 1395 1518 2119
Size: 100 340 381 330 107 137 123 601
Cuts at: 2119 2414 2635 2684 3050 3396
Size: 295 221 49 366 346
Fragments arranged by size:
601 381 366 346 340 330 295 221
137 123 107 100 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3345 3396
Size: 3345 51
EcoRI G'AATT_C
Cuts at: 0 884 3396
Size: 884 2512
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 445 550 642 851 1100 1425 3396
Size: 445 105 92 209 249 325 1971
Fragments arranged by size:
1971 445 325 249 209 105 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 270 302 635 667 1319 1351
Size: 25 32 213 32 333 32 652 32
Cuts at: 1351 1832 1864 2055 2087 3150 3182 3203 3235
Size: 481 32 191 32 1063 32 21 32
Cuts at: 3235 3396
Size: 161
Fragments arranged by size:
1063 652 481 333 213 191 161 32
32 32 32 32 32 32 32 25
21
HincII GTy'rAC
Cuts at: 0 320 1714 2990 3396
Size: 320 1394 1276 406
Fragments arranged by size:
1394 1276 406 320
HindIII A'AGCT_T
Cuts at: 0 218 1082 1807 2647 3396
Size: 218 864 725 840 749
Fragments arranged by size:
864 840 749 725 218
HpaI GTT'AAC
Cuts at: 0 2990 3396
Size: 2990 406
KpnI G_GTAC'C
Cuts at: 0 2158 2222 3396
Size: 2158 64 1174
Fragments arranged by size:
2158 1174 64
MscI TGG'CCA
Cuts at: 0 595 1655 3396
Size: 595 1060 1741
Fragments arranged by size:
1741 1060 595
MslI CAynn'nnrTG
Cuts at: 0 235 311 2015 2163 2325 3396
Size: 235 76 1704 148 162 1071
Fragments arranged by size:
1704 1071 235 162 148 76
MspA1I CmG'CkG
Cuts at: 0 3175 3396
Size: 3175 221
NcoI C'CATG_G
Cuts at: 0 2149 3396
Size: 2149 1247
NdeI CA'TA_TG
Cuts at: 0 1592 2799 3396
Size: 1592 1207 597
Fragments arranged by size:
1592 1207 597
NsiI A_TGCA'T
Cuts at: 0 1985 2170 3396
Size: 1985 185 1226
Fragments arranged by size:
1985 1226 185
NspI r_CATG'y
Cuts at: 0 2463 3396
Size: 2463 933
PciI A'CATG_T
Cuts at: 0 2459 3396
Size: 2459 937
PflMI CCAn_nnn'nTGG
Cuts at: 0 2228 3056 3396
Size: 2228 828 340
Fragments arranged by size:
2228 828 340
PshAI GACnn'nnGTC
Cuts at: 0 1709 3396
Size: 1709 1687
PsiI TTA'TAA
Cuts at: 0 766 865 2350 3396
Size: 766 99 1485 1046
Fragments arranged by size:
1485 1046 766 99
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 923 955 3396
Size: 923 32 2441
Fragments arranged by size:
2441 923 32
PstI C_TGCA'G
Cuts at: 0 785 1131 2242 2864 3396
Size: 785 346 1111 622 532
Fragments arranged by size:
1111 785 622 532 346
SapI GCTCTTCn'nnn_
Cuts at: 0 2660 3365 3396
Size: 2660 705 31
Fragments arranged by size:
2660 705 31
ScaI AGT'ACT
Cuts at: 0 2661 3396
Size: 2661 735
SexAI A'CCwGG_T
Cuts at: 0 3036 3396
Size: 3036 360
SfcI C'TryA_G
Cuts at: 0 339 781 1127 2238 2554 2611 2713 2751
Size: 339 442 346 1111 316 57 102 38
Cuts at: 2751 2860 3396
Size: 109 536
Fragments arranged by size:
1111 536 442 346 339 316 109 102
57 38
SmlI C'TyrA_G
Cuts at: 0 410 754 3396
Size: 410 344 2642
Fragments arranged by size:
2642 410 344
SspI AAT'ATT
Cuts at: 0 966 1303 1866 2034 2791 3396
Size: 966 337 563 168 757 605
Fragments arranged by size:
966 757 605 563 337 168
StuI AGG'CCT
Cuts at: 0 1584 2385 3396
Size: 1584 801 1011
Fragments arranged by size:
1584 1011 801
StyI C'CwwG_G
Cuts at: 0 138 1650 1656 2149 2487 3396
Size: 138 1512 6 493 338 909
Fragments arranged by size:
1512 909 493 338 138 6
SwaI ATTT'AAAT
Cuts at: 0 2189 3396
Size: 2189 1207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 306 3396
Size: 306 3090
TatI w'GTAC_w
Cuts at: 0 333 1269 1382 2659 2735 2864 3396
Size: 333 936 113 1277 76 129 532
Fragments arranged by size:
1277 936 532 333 129 113 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2007 2039 3396
Size: 2007 32 1357
Fragments arranged by size:
2007 1357 32
Tth111I GACn'n_nGTC
Cuts at: 0 442 3396
Size: 442 2954
XbaI T'CTAG_A
Cuts at: 0 1989 3396
Size: 1989 1407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1039 3396
Size: 1039 2357
XhoI C'TCGA_G
Cuts at: 0 410 3396
Size: 410 2986
XmnI GAAnn'nnTTC
Cuts at: 0 1408 1454 1767 3396
Size: 1408 46 313 1629
Fragments arranged by size:
1629 1408 313 46
Enzymes that do cut:
Acc65I AcuI AflII AflIII AleI ApoI AseI AvaI
AvrII BaeI BamHI BanI BbsI BciVI BclI BfrBI
BglII Bme1580I BmrI BplI BpmI BsaBI BsaXI BseRI
BsgI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrFI BstXI BstYI Bsu36I BtgI BtsI CspCI
DraI DraIII EaeI EarI Eco57MI EcoNI EcoRI FalI
Hin4I HincII HindIII HpaI KpnI MscI MslI MspA1I
NcoI NdeI NsiI NspI PciI PflMI PshAI PsiI
PsrI PstI SapI ScaI SexAI SfcI SmlI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI AscI AsiSI BanII BbeI
BbvCI BcgI BglI BlpI BmgBI BmtI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BseYI BsiEI BspEI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI
ClaI DrdI EagI EciI EcoICRI EcoO109I EcoRV FseI
FspI FspAI HaeII KasI MfeI MluI MmeI NaeI
NarI NgoMIV NheI NotI NruI PacI PasI PfoI
PmeI PmlI PpiI PpuMI PspOMI PspXI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI XmaI
ZraI