Restriction map for
pF1KE3350
(Linear) (Six-base) MAPSORT of: KIEE3350.seq Check: 9465 from: 1 to: 3108
KIEE3350.orf
With 182 enzymes: SgfI *
August 14, 2013 11:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 399 1111 2769 3108
Size: 399 712 1658 339
Fragments arranged by size:
1658 712 399 339
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 1532 1664 2264 3108
Size: 397 1135 132 600 844
Fragments arranged by size:
1135 844 600 397 132
AfeI AGC'GCT
Cuts at: 0 620 632 3108
Size: 620 12 2476
Fragments arranged by size:
2476 620 12
AflIII A'CryG_T
Cuts at: 0 351 2617 3108
Size: 351 2266 491
Fragments arranged by size:
2266 491 351
AgeI A'CCGG_T
Cuts at: 0 2347 3108
Size: 2347 761
AhdI GACnn_n'nnGTC
Cuts at: 0 792 3108
Size: 792 2316
AleI CACnn'nnGTG
Cuts at: 0 37 3108
Size: 37 3071
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 823 3108
Size: 823 2285
AlwNI CAG_nnn'CTG
Cuts at: 0 1398 2056 2080 2728 3108
Size: 1398 658 24 648 380
Fragments arranged by size:
1398 658 648 380 24
ApaLI G'TGCA_C
Cuts at: 0 2175 2471 3108
Size: 2175 296 637
Fragments arranged by size:
2175 637 296
ApoI r'AATT_y
Cuts at: 0 1271 1732 1752 1785 3097 3108
Size: 1271 461 20 33 1312 11
Fragments arranged by size:
1312 1271 461 33 20 11
AscI GG'CGCG_CC
Cuts at: 0 457 3108
Size: 457 2651
AvaI C'yCGr_G
Cuts at: 0 93 1322 1340 1632 3108
Size: 93 1229 18 292 1476
Fragments arranged by size:
1476 1229 292 93 18
BanI G'GyrC_C
Cuts at: 0 13 260 536 916 3108
Size: 13 247 276 380 2192
Fragments arranged by size:
2192 380 276 247 13
BanII G_rGCy'C
Cuts at: 0 60 386 3108
Size: 60 326 2722
Fragments arranged by size:
2722 326 60
BbeI G_GCGC'C
Cuts at: 0 17 264 3108
Size: 17 247 2844
Fragments arranged by size:
2844 247 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 944 2591 3108
Size: 944 1647 517
Fragments arranged by size:
1647 944 517
BbvCI CC'TCA_GC
Cuts at: 0 2828 3108
Size: 2828 280
BclI T'GATC_A
Cuts at: 0 944 3108
Size: 944 2164
BglI GCCn_nnn'nGGC
Cuts at: 0 333 451 931 3108
Size: 333 118 480 2177
Fragments arranged by size:
2177 480 333 118
BglII A'GATC_T
Cuts at: 0 2220 2862 3108
Size: 2220 642 246
Fragments arranged by size:
2220 642 246
BlpI GC'TnA_GC
Cuts at: 0 69 169 3108
Size: 69 100 2939
Fragments arranged by size:
2939 100 69
Bme1580I G_kGCm'C
Cuts at: 0 773 2179 2394 2475 3108
Size: 773 1406 215 81 633
Fragments arranged by size:
1406 773 633 215 81
BmgBI CAC'GTC
Cuts at: 0 354 1419 3009 3108
Size: 354 1065 1590 99
Fragments arranged by size:
1590 1065 354 99
BmrI ACTGGGnnnn_n'
Cuts at: 0 726 3108
Size: 726 2382
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2094 3108
Size: 2094 1014
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 619 1002 1444 2750 3108
Size: 391 228 383 442 1306 358
Fragments arranged by size:
1306 442 391 383 358 228
Bpu10I CC'TnA_GC
Cuts at: 0 1109 2828 3108
Size: 1109 1719 280
Fragments arranged by size:
1719 1109 280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 835 1214 3108
Size: 775 60 379 1894
Fragments arranged by size:
1894 775 379 60
BsaI GGTCTCn'nnnn_
Cuts at: 0 2796 3108
Size: 2796 312
BsaAI yAC'GTr
Cuts at: 0 288 705 3108
Size: 288 417 2403
Fragments arranged by size:
2403 417 288
BsaBI GATnn'nnATC
Cuts at: 0 1529 2690 3108
Size: 1529 1161 418
Fragments arranged by size:
1529 1161 418
BsaHI Gr'CG_yC
Cuts at: 0 14 212 261 3108
Size: 14 198 49 2847
Fragments arranged by size:
2847 198 49 14
BsaWI w'CCGG_w
Cuts at: 0 1015 2347 2505 3108
Size: 1015 1332 158 603
Fragments arranged by size:
1332 1015 603 158
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 187 217 3108
Size: 187 30 2891
Fragments arranged by size:
2891 187 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 81 772 1345 1384 2083 2756 3069 3108
Size: 81 691 573 39 699 673 313 39
Fragments arranged by size:
699 691 673 573 313 81 39 39
BseYI C'CCAG_C
Cuts at: 0 337 394 2063 3108
Size: 337 57 1669 1045
Fragments arranged by size:
1669 1045 337 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 745 763 1594 2723 3066 3108
Size: 274 471 18 831 1129 343 42
Fragments arranged by size:
1129 831 471 343 274 42 18
BsiEI CG_ry'CG
Cuts at: 0 29 50 308 3108
Size: 29 21 258 2800
Fragments arranged by size:
2800 258 29 21
BsiHKAI G_wGCw'C
Cuts at: 0 386 910 2179 2475 3108
Size: 386 524 1269 296 633
Fragments arranged by size:
1269 633 524 386 296
BsmI GAATG_Cn'
Cuts at: 0 1141 2091 3108
Size: 1141 950 1017
Fragments arranged by size:
1141 1017 950
Bsp1286I G_dGCh'C
Cuts at: 0 60 386 773 910 2179 2394 2475 3108
Size: 60 326 387 137 1269 215 81 633
Fragments arranged by size:
1269 633 387 326 215 137 81 60
BspEI T'CCGG_A
Cuts at: 0 2505 3108
Size: 2505 603
BspHI T'CATG_A
Cuts at: 0 1127 3108
Size: 1127 1981
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 399 758 1111 2769 3108
Size: 399 359 353 1658 339
Fragments arranged by size:
1658 399 359 353 339
BsrBI CCG'CTC
Cuts at: 0 366 1428 2858 3108
Size: 366 1062 1430 250
Fragments arranged by size:
1430 1062 366 250
BsrFI r'CCGG_y
Cuts at: 0 2347 3068 3108
Size: 2347 721 40
Fragments arranged by size:
2347 721 40
BssHII G'CGCG_C
Cuts at: 0 457 663 665 683 685 3108
Size: 457 206 2 18 2 2423
Fragments arranged by size:
2423 457 206 18 2 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2080 2239 2911 3108
Size: 2080 159 672 197
Fragments arranged by size:
2080 672 197 159
BstXI CCAn_nnnn'nTGG
Cuts at: 0 288 1896 2187 3108
Size: 288 1608 291 921
Fragments arranged by size:
1608 921 291 288
BstYI r'GATC_y
Cuts at: 0 2134 2220 2454 2862 3108
Size: 2134 86 234 408 246
Fragments arranged by size:
2134 408 246 234 86
Bsu36I CC'TnA_GG
Cuts at: 0 2870 3108
Size: 2870 238
BtgI C'CryG_G
Cuts at: 0 17 609 1052 3108
Size: 17 592 443 2056
Fragments arranged by size:
2056 592 443 17
BtsI GCAGTG_nn'
Cuts at: 0 1217 2519 2740 2897 3108
Size: 1217 1302 221 157 211
Fragments arranged by size:
1302 1217 221 211 157
DraI TTT'AAA
Cuts at: 0 1514 3108
Size: 1514 1594
DraIII CAC_nnn'GTG
Cuts at: 0 2429 2823 3108
Size: 2429 394 285
Fragments arranged by size:
2429 394 285
DrdI GACnn_nn'nnGTC
Cuts at: 0 2892 3108
Size: 2892 216
EaeI y'GGCC_r
Cuts at: 0 47 183 305 443 635 3070 3108
Size: 47 136 122 138 192 2435 38
Fragments arranged by size:
2435 192 138 136 122 47 38
EagI C'GGCC_G
Cuts at: 0 47 305 3108
Size: 47 258 2803
Fragments arranged by size:
2803 258 47
EarI CTCTTCn'nnn_
Cuts at: 0 526 1322 1477 1928 2258 3108
Size: 526 796 155 451 330 850
Fragments arranged by size:
850 796 526 451 330 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 629 1617 3108
Size: 629 988 1491
Fragments arranged by size:
1491 988 629
EcoICRI GAG'CTC
Cuts at: 0 384 3108
Size: 384 2724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 397 619 1002 1444 1532 1664 2264
Size: 391 6 222 383 442 88 132 600
Cuts at: 2264 2750 3108
Size: 486 358
Fragments arranged by size:
600 486 442 391 383 358 222 132
88 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 754 3108
Size: 754 2354
EcoO109I rG'GnC_Cy
Cuts at: 0 465 786 1925 3108
Size: 465 321 1139 1183
Fragments arranged by size:
1183 1139 465 321
EcoRI G'AATT_C
Cuts at: 0 3097 3108
Size: 3097 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 972 3108
Size: 972 2136
HaeII r_GCGC'y
Cuts at: 0 17 264 395 622 634 3108
Size: 17 247 131 227 12 2474
Fragments arranged by size:
2474 247 227 131 17 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 778 810 1303 1335 1520 1552
Size: 187 32 559 32 493 32 185 32
Cuts at: 1552 1818 1850 2028 2060 2198 2230 2270 2302
Size: 266 32 178 32 138 32 40 32
Cuts at: 2302 2487 2519 3108
Size: 185 32 589
Fragments arranged by size:
589 559 493 266 187 185 185 178
138 40 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 714 1193 1676 1862 3108
Size: 714 479 483 186 1246
Fragments arranged by size:
1246 714 483 479 186
KasI G'GCGC_C
Cuts at: 0 13 260 3108
Size: 13 247 2848
Fragments arranged by size:
2848 247 13
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2719 3108
Size: 2719 389
MslI CAynn'nnrTG
Cuts at: 0 37 901 1648 1795 2107 3108
Size: 37 864 747 147 312 1001
Fragments arranged by size:
1001 864 747 312 147 37
MspA1I CmG'CkG
Cuts at: 0 19 189 258 611 640 1398 2295 2901
Size: 19 170 69 353 29 758 897 606
Cuts at: 2901 3108
Size: 207
Fragments arranged by size:
897 758 606 353 207 170 69 29
19
NaeI GCC'GGC
Cuts at: 0 3070 3108
Size: 3070 38
NarI GG'CG_CC
Cuts at: 0 14 261 3108
Size: 14 247 2847
Fragments arranged by size:
2847 247 14
NgoMIV G'CCGG_C
Cuts at: 0 3068 3108
Size: 3068 40
NspI r_CATG'y
Cuts at: 0 744 2621 3108
Size: 744 1877 487
Fragments arranged by size:
1877 744 487
PasI CC'CwG_GG
Cuts at: 0 2394 3108
Size: 2394 714
PciI A'CATG_T
Cuts at: 0 2617 3108
Size: 2617 491
PflMI CCAn_nnn'nTGG
Cuts at: 0 192 441 2533 3108
Size: 192 249 2092 575
Fragments arranged by size:
2092 575 249 192
PmlI CAC'GTG
Cuts at: 0 705 3108
Size: 705 2403
PpuMI rG'GwC_Cy
Cuts at: 0 465 786 1925 3108
Size: 465 321 1139 1183
Fragments arranged by size:
1183 1139 465 321
PstI C_TGCA'G
Cuts at: 0 575 1355 2052 2441 3108
Size: 575 780 697 389 667
Fragments arranged by size:
780 697 667 575 389
PvuII CAG'CTG
Cuts at: 0 189 1398 2901 3108
Size: 189 1209 1503 207
Fragments arranged by size:
1503 1209 207 189
SacI G_AGCT'C
Cuts at: 0 386 3108
Size: 386 2722
SacII CC_GC'GG
Cuts at: 0 20 612 3108
Size: 20 592 2496
Fragments arranged by size:
2496 592 20
SapI GCTCTTCn'nnn_
Cuts at: 0 526 1928 3108
Size: 526 1402 1180
Fragments arranged by size:
1402 1180 526
SfcI C'TryA_G
Cuts at: 0 571 1351 2048 2437 2608 3108
Size: 571 780 697 389 171 500
Fragments arranged by size:
780 697 571 500 389 171
SfoI GGC'GCC
Cuts at: 0 15 262 3108
Size: 15 247 2846
Fragments arranged by size:
2846 247 15
SgrAI Cr'CCGG_yG
Cuts at: 0 2347 3108
Size: 2347 761
SmaI CCC'GGG
Cuts at: 0 1324 3108
Size: 1324 1784
SmlI C'TyrA_G
Cuts at: 0 790 814 1229 3108
Size: 790 24 415 1879
Fragments arranged by size:
1879 790 415 24
SphI G_CATG'C
Cuts at: 0 744 3108
Size: 744 2364
StuI AGG'CCT
Cuts at: 0 763 1579 2002 3108
Size: 763 816 423 1106
Fragments arranged by size:
1106 816 763 423
StyI C'CwwG_G
Cuts at: 0 1472 1855 1895 2258 2782 2963 3108
Size: 1472 383 40 363 524 181 145
Fragments arranged by size:
1472 524 383 363 181 145 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 43 2162 2811 2962 3108
Size: 43 2119 649 151 146
Fragments arranged by size:
2119 649 151 146 43
Tth111I GACn'n_nGTC
Cuts at: 0 2679 3108
Size: 2679 429
XmaI C'CCGG_G
Cuts at: 0 1322 3108
Size: 1322 1786
XmnI GAAnn'nnTTC
Cuts at: 0 1238 3097 3108
Size: 1238 1859 11
Fragments arranged by size:
1859 1238 11
Enzymes that do cut:
AarI AcuI AfeI AflIII AgeI AhdI AleI AlfI
AlwNI ApaLI ApoI AscI AvaI BanI BanII BbeI
BbsI BbvCI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII
BstAPI BstXI BstYI Bsu36I BtgI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MmeI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PmlI PpuMI PstI PvuII SacI SacII SapI
SfcI SfoI SgrAI SmaI SmlI SphI StuI StyI
TaqII Tth111I XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AloI ApaI AseI
AsiSI AvrII BaeI BamHI BcgI BciVI BfrBI BmtI
BsiWI BsmBI BsrDI BsrGI BssSI BstBI BstEII BstZ17I
BtgZI ClaI CspCI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MscI NcoI NdeI NheI
NotI NruI NsiI PacI PfoI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SrfI
SspI SwaI TatI TstI XbaI XcmI XhoI ZraI