Restriction map for
pF1KE3268
(Linear) (Six-base) MAPSORT of: KIEE3268.seq Check: 9317 from: 1 to: 1998
KIEE3268.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 0 1434 1998
Size: 1434 564
AclI AA'CG_TT
Cuts at: 0 1623 1998
Size: 1623 375
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 950 1455 1998
Size: 950 505 543
Fragments arranged by size:
950 543 505
AhdI GACnn_n'nnGTC
Cuts at: 0 1447 1998
Size: 1447 551
AleI CACnn'nnGTG
Cuts at: 0 907 1998
Size: 907 1091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1363 1998
Size: 1363 635
AlwNI CAG_nnn'CTG
Cuts at: 0 85 817 1998
Size: 85 732 1181
Fragments arranged by size:
1181 732 85
ApoI r'AATT_y
Cuts at: 0 480 531 681 732 1189 1998
Size: 480 51 150 51 457 809
Fragments arranged by size:
809 480 457 150 51 51
BanI G'GyrC_C
Cuts at: 0 909 1998
Size: 909 1089
BanII G_rGCy'C
Cuts at: 0 1157 1998
Size: 1157 841
BbsI GAAGACnn'nnnn_
Cuts at: 0 356 989 1998
Size: 356 633 1009
Fragments arranged by size:
1009 633 356
BbvCI CC'TCA_GC
Cuts at: 0 581 1998
Size: 581 1417
BciVI GTATCCnnnnn_n'
Cuts at: 0 1262 1998
Size: 1262 736
BclI T'GATC_A
Cuts at: 0 87 135 1967 1998
Size: 87 48 1832 31
Fragments arranged by size:
1832 87 48 31
BlpI GC'TnA_GC
Cuts at: 0 47 1693 1998
Size: 47 1646 305
Fragments arranged by size:
1646 305 47
Bme1580I G_kGCm'C
Cuts at: 0 504 1998
Size: 504 1494
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 983 1998
Size: 983 1015
Bpu10I CC'TnA_GC
Cuts at: 0 581 1998
Size: 581 1417
BsaI GGTCTCn'nnnn_
Cuts at: 0 1057 1998
Size: 1057 941
BsaAI yAC'GTr
Cuts at: 0 1642 1998
Size: 1642 356
BsaBI GATnn'nnATC
Cuts at: 0 86 311 1555 1998
Size: 86 225 1244 443
Fragments arranged by size:
1244 443 225 86
BsaHI Gr'CG_yC
Cuts at: 0 32 1998
Size: 32 1966
BsaWI w'CCGG_w
Cuts at: 0 1909 1998
Size: 1909 89
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 508 586 631 1063 1744 1747 1759 1998
Size: 508 78 45 432 681 3 12 239
Fragments arranged by size:
681 508 432 239 78 45 12 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 971 1998
Size: 971 1027
BsiEI CG_ry'CG
Cuts at: 0 649 1998
Size: 649 1349
BsmI GAATG_Cn'
Cuts at: 0 102 777 873 1591 1790 1998
Size: 102 675 96 718 199 208
Fragments arranged by size:
718 675 208 199 102 96
Bsp1286I G_dGCh'C
Cuts at: 0 504 1157 1998
Size: 504 653 841
Fragments arranged by size:
841 653 504
BspEI T'CCGG_A
Cuts at: 0 1909 1998
Size: 1909 89
BsrDI GCAATG_nn'
Cuts at: 0 1314 1365 1998
Size: 1314 51 633
Fragments arranged by size:
1314 633 51
BsrFI r'CCGG_y
Cuts at: 0 648 1998
Size: 648 1350
BsrGI T'GTAC_A
Cuts at: 0 1376 1998
Size: 1376 622
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1707 1758 1998
Size: 1707 51 240
Fragments arranged by size:
1707 240 51
BstYI r'GATC_y
Cuts at: 0 15 70 508 1998
Size: 15 55 438 1490
Fragments arranged by size:
1490 438 55 15
Bsu36I CC'TnA_GG
Cuts at: 0 1031 1998
Size: 1031 967
BtgI C'CryG_G
Cuts at: 0 932 1998
Size: 932 1066
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1988 1998
Size: 1988 10
BtsI GCAGTG_nn'
Cuts at: 0 834 1387 1998
Size: 834 553 611
Fragments arranged by size:
834 611 553
ClaI AT'CG_AT
Cuts at: 0 1719 1998
Size: 1719 279
DraI TTT'AAA
Cuts at: 0 1455 1998
Size: 1455 543
EaeI y'GGCC_r
Cuts at: 0 646 1998
Size: 646 1352
EagI C'GGCC_G
Cuts at: 0 646 1998
Size: 646 1352
EarI CTCTTCn'nnn_
Cuts at: 0 25 713 785 969 1040 1973 1998
Size: 25 688 72 184 71 933 25
Fragments arranged by size:
933 688 184 72 71 25 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1998
Size: 19 1979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 950 983 1455 1998
Size: 950 33 472 543
Fragments arranged by size:
950 543 472 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 998 1998
Size: 998 1000
EcoRI G'AATT_C
Cuts at: 0 531 681 1998
Size: 531 150 1317
Fragments arranged by size:
1317 531 150
EcoRV GAT'ATC
Cuts at: 0 960 1998
Size: 960 1038
HaeII r_GCGC'y
Cuts at: 0 1662 1700 1998
Size: 1662 38 298
Fragments arranged by size:
1662 298 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 223 244 255 276 793 825
Size: 55 32 136 21 11 21 517 32
Cuts at: 825 946 978 1433 1465 1796 1822 1828 1854
Size: 121 32 455 32 331 26 6 26
Cuts at: 1854 1998
Size: 144
Fragments arranged by size:
517 455 331 144 136 121 55 32
32 32 32 26 26 21 21 11
6
HindIII A'AGCT_T
Cuts at: 0 266 1998
Size: 266 1732
MslI CAynn'nnrTG
Cuts at: 0 221 419 782 907 1600 1998
Size: 221 198 363 125 693 398
Fragments arranged by size:
693 398 363 221 198 125
MspA1I CmG'CkG
Cuts at: 0 708 1686 1998
Size: 708 978 312
Fragments arranged by size:
978 708 312
NcoI C'CATG_G
Cuts at: 0 932 1998
Size: 932 1066
NspI r_CATG'y
Cuts at: 0 1344 1998
Size: 1344 654
PflMI CCAn_nnn'nTGG
Cuts at: 0 1570 1998
Size: 1570 428
PfoI T'CCnGG_A
Cuts at: 0 999 1998
Size: 999 999
PshAI GACnn'nnGTC
Cuts at: 0 356 1998
Size: 356 1642
PsiI TTA'TAA
Cuts at: 0 1417 1998
Size: 1417 581
PstI C_TGCA'G
Cuts at: 0 530 1394 1998
Size: 530 864 604
Fragments arranged by size:
864 604 530
PvuII CAG'CTG
Cuts at: 0 708 1998
Size: 708 1290
SexAI A'CCwGG_T
Cuts at: 0 1228 1998
Size: 1228 770
SfcI C'TryA_G
Cuts at: 0 526 1390 1435 1998
Size: 526 864 45 563
Fragments arranged by size:
864 563 526 45
SnaBI TAC'GTA
Cuts at: 0 1642 1998
Size: 1642 356
StuI AGG'CCT
Cuts at: 0 579 757 1998
Size: 579 178 1241
Fragments arranged by size:
1241 579 178
StyI C'CwwG_G
Cuts at: 0 932 1998
Size: 932 1066
TatI w'GTAC_w
Cuts at: 0 1376 1998
Size: 1376 622
Tth111I GACn'n_nGTC
Cuts at: 0 1515 1998
Size: 1515 483
XmnI GAAnn'nnTTC
Cuts at: 0 1815 1998
Size: 1815 183
Enzymes that do cut:
AccI AclI AcuI AhdI AleI AlfI AlwNI ApoI
BanI BanII BbsI BbvCI BciVI BclI BlpI Bme1580I
BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BsgI BsiEI BsmI Bsp1286I BspEI BsrDI BsrFI BsrGI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV
HaeII Hin4I HindIII MslI MspA1I NcoI NspI PflMI
PfoI PshAI PsiI PstI PvuII SexAI SfcI SnaBI
StuI StyI TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BcgI BfrBI BglI BglII BmgBI BmrI
BmtI BplI BpuEI BsaXI BseYI BsiHKAI BsiWI BsmBI
BspHI BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII
BstZ17I CspCI DraIII DrdI EcoICRI EcoO109I FalI FseI
FspI FspAI HincII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PpuMI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SrfI SspI SwaI
TaqII TstI XbaI XcmI XhoI XmaI ZraI