Restriction map for
pF1KE3218
(Linear) (Six-base) MAPSORT of: KIEE3218.seq Check: 7836 from: 1 to: 1485
KIEE3218.orf
With 182 enzymes: SgfI *
April 23, 2019 13:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 640 882 1145 1485
Size: 104 536 242 263 340
Fragments arranged by size:
536 340 263 242 104
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 331 909 1485
Size: 331 578 576
Fragments arranged by size:
578 576 331
ApaLI G'TGCA_C
Cuts at: 0 1264 1485
Size: 1264 221
ApoI r'AATT_y
Cuts at: 0 681 1485
Size: 681 804
AscI GG'CGCG_CC
Cuts at: 0 1430 1485
Size: 1430 55
AvaI C'yCGr_G
Cuts at: 0 101 1424 1485
Size: 101 1323 61
Fragments arranged by size:
1323 101 61
BamHI G'GATC_C
Cuts at: 0 492 842 1130 1485
Size: 492 350 288 355
Fragments arranged by size:
492 355 350 288
BanI G'GyrC_C
Cuts at: 0 850 1485
Size: 850 635
BanII G_rGCy'C
Cuts at: 0 1423 1485
Size: 1423 62
BbsI GAAGACnn'nnnn_
Cuts at: 0 33 92 341 628 1485
Size: 33 59 249 287 857
Fragments arranged by size:
857 287 249 59 33
BfrBI ATG'CAT
Cuts at: 0 373 1485
Size: 373 1112
BglI GCCn_nnn'nGGC
Cuts at: 0 1378 1485
Size: 1378 107
Bme1580I G_kGCm'C
Cuts at: 0 1268 1485
Size: 1268 217
BmrI ACTGGGnnnn_n'
Cuts at: 0 1385 1485
Size: 1385 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 740 1341 1485
Size: 310 430 601 144
Fragments arranged by size:
601 430 310 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 1485
Size: 619 866
BsaAI yAC'GTr
Cuts at: 0 1233 1485
Size: 1233 252
BsaWI w'CCGG_w
Cuts at: 0 671 839 1485
Size: 671 168 646
Fragments arranged by size:
671 646 168
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1357 1387 1459 1485
Size: 1357 30 72 26
Fragments arranged by size:
1357 72 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 171 998 1485
Size: 171 827 487
Fragments arranged by size:
827 487 171
BseYI C'CCAG_C
Cuts at: 0 172 399 692 777 986 1413 1485
Size: 172 227 293 85 209 427 72
Fragments arranged by size:
427 293 227 209 172 85 72
BsiEI CG_ry'CG
Cuts at: 0 516 1485
Size: 516 969
BsiHKAI G_wGCw'C
Cuts at: 0 1268 1485
Size: 1268 217
BsmBI CGTCTCn'nnnn_
Cuts at: 0 454 1485
Size: 454 1031
Bsp1286I G_dGCh'C
Cuts at: 0 1268 1423 1485
Size: 1268 155 62
Fragments arranged by size:
1268 155 62
BspEI T'CCGG_A
Cuts at: 0 839 1485
Size: 839 646
BspHI T'CATG_A
Cuts at: 0 1104 1485
Size: 1104 381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 617 1259 1485
Size: 617 642 226
Fragments arranged by size:
642 617 226
BsrBI CCG'CTC
Cuts at: 0 118 1031 1485
Size: 118 913 454
Fragments arranged by size:
913 454 118
BsrFI r'CCGG_y
Cuts at: 0 551 815 950 1485
Size: 551 264 135 535
Fragments arranged by size:
551 535 264 135
BssHII G'CGCG_C
Cuts at: 0 1430 1485
Size: 1430 55
BssSI C'ACGA_G
Cuts at: 0 439 1267 1485
Size: 439 828 218
Fragments arranged by size:
828 439 218
BstAPI GCAn_nnn'nTGC
Cuts at: 0 830 1485
Size: 830 655
BstBI TT'CG_AA
Cuts at: 0 483 1485
Size: 483 1002
BstYI r'GATC_y
Cuts at: 0 492 842 1130 1485
Size: 492 350 288 355
Fragments arranged by size:
492 355 350 288
Bsu36I CC'TnA_GG
Cuts at: 0 1195 1485
Size: 1195 290
BtgI C'CryG_G
Cuts at: 0 132 1485
Size: 132 1353
BtsI GCAGTG_nn'
Cuts at: 0 1121 1298 1485
Size: 1121 177 187
Fragments arranged by size:
1121 187 177
DraIII CAC_nnn'GTG
Cuts at: 0 1208 1485
Size: 1208 277
EaeI y'GGCC_r
Cuts at: 0 129 1485
Size: 129 1356
EarI CTCTTCn'nnn_
Cuts at: 0 562 1485
Size: 562 923
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 489 1485
Size: 489 996
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 310 640 740 882 1145 1341 1485
Size: 104 206 330 100 142 263 196 144
Fragments arranged by size:
330 263 206 196 144 142 104 100
EcoO109I rG'GnC_Cy
Cuts at: 0 176 921 1191 1461 1485
Size: 176 745 270 270 24
Fragments arranged by size:
745 270 270 176 24
EcoRI G'AATT_C
Cuts at: 0 681 1485
Size: 681 804
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 136 162 168 509 541 1485
Size: 130 6 26 6 341 32 944
Fragments arranged by size:
944 341 130 32 26 6 6
HincII GTy'rAC
Cuts at: 0 155 929 1485
Size: 155 774 556
Fragments arranged by size:
774 556 155
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 1485
Size: 561 924
MslI CAynn'nnrTG
Cuts at: 0 137 1235 1485
Size: 137 1098 250
Fragments arranged by size:
1098 250 137
MspA1I CmG'CkG
Cuts at: 0 172 399 455 1485
Size: 172 227 56 1030
Fragments arranged by size:
1030 227 172 56
NsiI A_TGCA'T
Cuts at: 0 375 1485
Size: 375 1110
NspI r_CATG'y
Cuts at: 0 828 1264 1485
Size: 828 436 221
Fragments arranged by size:
828 436 221
PfoI T'CCnGG_A
Cuts at: 0 326 1485
Size: 326 1159
PmlI CAC'GTG
Cuts at: 0 1233 1485
Size: 1233 252
PpuMI rG'GwC_Cy
Cuts at: 0 176 921 1191 1461 1485
Size: 176 745 270 270 24
Fragments arranged by size:
745 270 270 176 24
PstI C_TGCA'G
Cuts at: 0 835 1485
Size: 835 650
PvuII CAG'CTG
Cuts at: 0 172 399 455 1485
Size: 172 227 56 1030
Fragments arranged by size:
1030 227 172 56
SapI GCTCTTCn'nnn_
Cuts at: 0 562 1485
Size: 562 923
SfcI C'TryA_G
Cuts at: 0 831 1479 1485
Size: 831 648 6
Fragments arranged by size:
831 648 6
SmaI CCC'GGG
Cuts at: 0 103 1426 1485
Size: 103 1323 59
Fragments arranged by size:
1323 103 59
SmlI C'TyrA_G
Cuts at: 0 634 1485
Size: 634 851
SphI G_CATG'C
Cuts at: 0 828 1485
Size: 828 657
StyI C'CwwG_G
Cuts at: 0 61 503 1376 1485
Size: 61 442 873 109
Fragments arranged by size:
873 442 109 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 530 818 1485
Size: 530 288 667
Fragments arranged by size:
667 530 288
TatI w'GTAC_w
Cuts at: 0 901 1485
Size: 901 584
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 700 732 834 866 940 957 972 989
Size: 700 32 102 32 74 17 15 17
Cuts at: 989 1271 1303 1485
Size: 282 32 182
Fragments arranged by size:
700 282 182 102 74 32 32 32
17 17 15
XmaI C'CCGG_G
Cuts at: 0 101 1424 1485
Size: 101 1323 61
Fragments arranged by size:
1323 101 61
Enzymes that do cut:
AcuI AlfI ApaLI ApoI AscI AvaI BamHI BanI
BanII BbsI BfrBI BglI Bme1580I BmrI BpmI BpuEI
BsaAI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtsI DraIII EaeI
EarI EciI Eco57MI EcoO109I EcoRI Hin4I HincII MmeI
MslI MspA1I NsiI NspI PfoI PmlI PpuMI PstI
PvuII SapI SfcI SmaI SmlI SphI StyI TaqII
TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BciVI BclI BglII
BlpI BmgBI BmtI BplI Bpu10I BsaI BsaBI BsaHI
BsgI BsiWI BsmI BsrDI BsrGI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI StuI SwaI Tth111I XbaI XcmI XhoI XmnI
ZraI