Restriction map for
pF1KE3209
(Linear) (Six-base) MAPSORT of: KIEE3209.seq Check: 8388 from: 1 to: 1434
KIEE3209.orf
With 182 enzymes: SgfI *
June 13, 2018 19:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 521 1047 1434
Size: 521 526 387
Fragments arranged by size:
526 521 387
AccI GT'mk_AC
Cuts at: 0 326 1434
Size: 326 1108
Acc65I G'GTAC_C
Cuts at: 0 1136 1434
Size: 1136 298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 455 1162 1411 1434
Size: 288 167 707 249 23
Fragments arranged by size:
707 288 249 167 23
AfeI AGC'GCT
Cuts at: 0 434 1434
Size: 434 1000
AflIII A'CryG_T
Cuts at: 0 1406 1434
Size: 1406 28
AlwNI CAG_nnn'CTG
Cuts at: 0 48 513 1062 1434
Size: 48 465 549 372
Fragments arranged by size:
549 465 372 48
ApaI G_GGCC'C
Cuts at: 0 41 111 205 496 890 1434
Size: 41 70 94 291 394 544
Fragments arranged by size:
544 394 291 94 70 41
AvaI C'yCGr_G
Cuts at: 0 27 34 428 436 693 820 1434
Size: 27 7 394 8 257 127 614
Fragments arranged by size:
614 394 257 127 27 8 7
BanI G'GyrC_C
Cuts at: 0 173 579 699 1136 1434
Size: 173 406 120 437 298
Fragments arranged by size:
437 406 298 173 120
BanII G_rGCy'C
Cuts at: 0 41 111 205 496 767 827 890 1322
Size: 41 70 94 291 271 60 63 432
Cuts at: 1322 1434
Size: 112
Fragments arranged by size:
432 291 271 112 94 70 63 60
41
BbeI G_GCGC'C
Cuts at: 0 583 1434
Size: 583 851
BbsI GAAGACnn'nnnn_
Cuts at: 0 1084 1434
Size: 1084 350
BglI GCCn_nnn'nGGC
Cuts at: 0 266 423 647 877 1434
Size: 266 157 224 230 557
Fragments arranged by size:
557 266 230 224 157
Bme1580I G_kGCm'C
Cuts at: 0 41 111 205 496 704 890 1434
Size: 41 70 94 291 208 186 544
Fragments arranged by size:
544 291 208 186 94 70 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 893 1434
Size: 893 541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 877 1434
Size: 151 726 557
Fragments arranged by size:
726 557 151
BsaI GGTCTCn'nnnn_
Cuts at: 0 809 1434
Size: 809 625
BsaBI GATnn'nnATC
Cuts at: 0 1373 1434
Size: 1373 61
BsaHI Gr'CG_yC
Cuts at: 0 580 1434
Size: 580 854
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 649 697 1434
Size: 649 48 737
Fragments arranged by size:
737 649 48
BseYI C'CCAG_C
Cuts at: 0 41 205 668 709 973 1434
Size: 41 164 463 41 264 461
Fragments arranged by size:
463 461 264 164 41 41
BsiEI CG_ry'CG
Cuts at: 0 137 191 277 1434
Size: 137 54 86 1157
Fragments arranged by size:
1157 137 86 54
BsiHKAI G_wGCw'C
Cuts at: 0 1129 1434
Size: 1129 305
Bsp1286I G_dGCh'C
Cuts at: 0 41 111 205 496 704 767 827 890
Size: 41 70 94 291 208 63 60 63
Cuts at: 890 1129 1322 1434
Size: 239 193 112
Fragments arranged by size:
291 239 208 193 112 94 70 63
63 60 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 521 1047 1434
Size: 521 526 387
Fragments arranged by size:
526 521 387
BsrBI CCG'CTC
Cuts at: 0 16 1434
Size: 16 1418
BsrFI r'CCGG_y
Cuts at: 0 94 260 1434
Size: 94 166 1174
Fragments arranged by size:
1174 166 94
BssHII G'CGCG_C
Cuts at: 0 269 933 1434
Size: 269 664 501
Fragments arranged by size:
664 501 269
BstXI CCAn_nnnn'nTGG
Cuts at: 0 576 750 774 1434
Size: 576 174 24 660
Fragments arranged by size:
660 576 174 24
BstYI r'GATC_y
Cuts at: 0 162 1434
Size: 162 1272
BtgI C'CryG_G
Cuts at: 0 131 454 561 569 1412 1434
Size: 131 323 107 8 843 22
Fragments arranged by size:
843 323 131 107 22 8
BtsI GCAGTG_nn'
Cuts at: 0 124 785 1111 1434
Size: 124 661 326 323
Fragments arranged by size:
661 326 323 124
EaeI y'GGCC_r
Cuts at: 0 92 134 188 274 389 542 752 1000
Size: 92 42 54 86 115 153 210 248
Cuts at: 1000 1046 1434
Size: 46 388
Fragments arranged by size:
388 248 210 153 115 92 86 54
46 42
EagI C'GGCC_G
Cuts at: 0 134 188 274 1434
Size: 134 54 86 1160
Fragments arranged by size:
1160 134 86 54
EarI CTCTTCn'nnn_
Cuts at: 0 523 1366 1434
Size: 523 843 68
Fragments arranged by size:
843 523 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 654 1434
Size: 654 780
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 288 455 877 1162 1411 1434
Size: 151 137 167 422 285 249 23
Fragments arranged by size:
422 285 249 167 151 137 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 601 1434
Size: 601 833
EcoO109I rG'GnC_Cy
Cuts at: 0 31 38 107 108 202 690 887 1356
Size: 31 7 69 1 94 488 197 469
Cuts at: 1356 1434
Size: 78
Fragments arranged by size:
488 469 197 94 78 69 31 7
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1167 1434
Size: 1167 267
HaeII r_GCGC'y
Cuts at: 0 212 436 583 991 1434
Size: 212 224 147 408 443
Fragments arranged by size:
443 408 224 212 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1236 1268 1434
Size: 1236 32 166
Fragments arranged by size:
1236 166 32
HincII GTy'rAC
Cuts at: 0 327 1434
Size: 327 1107
KasI G'GCGC_C
Cuts at: 0 579 1434
Size: 579 855
KpnI G_GTAC'C
Cuts at: 0 1140 1434
Size: 1140 294
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1283 1434
Size: 1283 151
MscI TGG'CCA
Cuts at: 0 754 1434
Size: 754 680
MslI CAynn'nnrTG
Cuts at: 0 1411 1434
Size: 1411 23
MspA1I CmG'CkG
Cuts at: 0 45 133 171 456 483 563 672 850
Size: 45 88 38 285 27 80 109 178
Cuts at: 850 966 1434
Size: 116 468
Fragments arranged by size:
468 285 178 116 109 88 80 45
38 27
NaeI GCC'GGC
Cuts at: 0 96 262 1434
Size: 96 166 1172
Fragments arranged by size:
1172 166 96
NarI GG'CG_CC
Cuts at: 0 580 1434
Size: 580 854
NcoI C'CATG_G
Cuts at: 0 569 1434
Size: 569 865
NgoMIV G'CCGG_C
Cuts at: 0 94 260 1434
Size: 94 166 1174
Fragments arranged by size:
1174 166 94
NotI GC'GGCC_GC
Cuts at: 0 134 188 1434
Size: 134 54 1246
Fragments arranged by size:
1246 134 54
NspI r_CATG'y
Cuts at: 0 237 1226 1410 1434
Size: 237 989 184 24
Fragments arranged by size:
989 237 184 24
PasI CC'CwG_GG
Cuts at: 0 178 489 1434
Size: 178 311 945
Fragments arranged by size:
945 311 178
PciI A'CATG_T
Cuts at: 0 1406 1434
Size: 1406 28
PflMI CCAn_nnn'nTGG
Cuts at: 0 219 1434
Size: 219 1215
PfoI T'CCnGG_A
Cuts at: 0 83 893 1129 1364 1434
Size: 83 810 236 235 70
Fragments arranged by size:
810 236 235 83 70
PpuMI rG'GwC_Cy
Cuts at: 0 31 690 1356 1434
Size: 31 659 666 78
Fragments arranged by size:
666 659 78 31
PspOMI G'GGCC_C
Cuts at: 0 37 107 201 492 886 1434
Size: 37 70 94 291 394 548
Fragments arranged by size:
548 394 291 94 70 37
PspXI vC'TCGA_Gb
Cuts at: 0 428 436 1434
Size: 428 8 998
Fragments arranged by size:
998 428 8
PstI C_TGCA'G
Cuts at: 0 152 791 1434
Size: 152 639 643
Fragments arranged by size:
643 639 152
PvuII CAG'CTG
Cuts at: 0 45 672 966 1434
Size: 45 627 294 468
Fragments arranged by size:
627 468 294 45
RsrII CG'GwC_CG
Cuts at: 0 294 1434
Size: 294 1140
SacII CC_GC'GG
Cuts at: 0 134 457 564 1434
Size: 134 323 107 870
Fragments arranged by size:
870 323 134 107
SalI G'TCGA_C
Cuts at: 0 325 1434
Size: 325 1109
SanDI GG'GwC_CC
Cuts at: 0 31 690 1434
Size: 31 659 744
Fragments arranged by size:
744 659 31
SapI GCTCTTCn'nnn_
Cuts at: 0 523 1434
Size: 523 911
SbfI CC_TGCA'GG
Cuts at: 0 152 1434
Size: 152 1282
SfcI C'TryA_G
Cuts at: 0 148 787 1434
Size: 148 639 647
Fragments arranged by size:
647 639 148
SfoI GGC'GCC
Cuts at: 0 581 1434
Size: 581 853
SmaI CCC'GGG
Cuts at: 0 29 36 695 1434
Size: 29 7 659 739
Fragments arranged by size:
739 659 29 7
SmlI C'TyrA_G
Cuts at: 0 428 436 1434
Size: 428 8 998
Fragments arranged by size:
998 428 8
StyI C'CwwG_G
Cuts at: 0 569 608 1350 1434
Size: 569 39 742 84
Fragments arranged by size:
742 569 84 39
TatI w'GTAC_w
Cuts at: 0 254 1434
Size: 254 1180
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 935 967 1434
Size: 935 32 467
Fragments arranged by size:
935 467 32
Tth111I GACn'n_nGTC
Cuts at: 0 220 1434
Size: 220 1214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 175 318 1410 1434
Size: 175 143 1092 24
Fragments arranged by size:
1092 175 143 24
XhoI C'TCGA_G
Cuts at: 0 428 436 1434
Size: 428 8 998
Fragments arranged by size:
998 428 8
XmaI C'CCGG_G
Cuts at: 0 27 34 693 1434
Size: 27 7 659 741
Fragments arranged by size:
741 659 27 7
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AlwNI ApaI
AvaI BanI BanII BbeI BbsI BglI Bme1580I BmrI
BpmI BsaI BsaBI BsaHI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BssHII BstXI BstYI BtgI
BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PasI PciI PflMI PfoI PpuMI PspOMI PspXI PstI
PvuII RsrII SacII SalI SanDI SapI SbfI SfcI
SfoI SmaI SmlI StyI TatI TstI Tth111I XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AleI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaAI BsaWI BsaXI BsgI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EcoICRI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI MfeI MluI NdeI NheI NruI
NsiI PacI PmeI PmlI PpiI PshAI PsiI PsrI
PvuI SacI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII XbaI
XmnI ZraI