Restriction map for
pF1KE3145
(Linear) (Six-base) MAPSORT of: KIEE3145.seq Check: 6051 from: 1 to: 1062
KIEE3145.orf
With 182 enzymes: SgfI *
December 4, 2012 11:59 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 570 1062
Size: 52 518 492
Fragments arranged by size:
518 492 52
AlwNI CAG_nnn'CTG
Cuts at: 0 493 758 1062
Size: 493 265 304
Fragments arranged by size:
493 304 265
ApoI r'AATT_y
Cuts at: 0 404 930 953 1062
Size: 404 526 23 109
Fragments arranged by size:
526 404 109 23
AvaI C'yCGr_G
Cuts at: 0 530 1062
Size: 530 532
BbsI GAAGACnn'nnnn_
Cuts at: 0 713 1062
Size: 713 349
BbvCI CC'TCA_GC
Cuts at: 0 371 1062
Size: 371 691
BfrBI ATG'CAT
Cuts at: 0 197 659 729 1062
Size: 197 462 70 333
Fragments arranged by size:
462 333 197 70
BglII A'GATC_T
Cuts at: 0 613 1062
Size: 613 449
BlpI GC'TnA_GC
Cuts at: 0 80 1062
Size: 80 982
BmrI ACTGGGnnnn_n'
Cuts at: 0 881 1062
Size: 881 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1062
Size: 76 986
Bpu10I CC'TnA_GC
Cuts at: 0 371 1062
Size: 371 691
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 659 1062
Size: 320 339 403
Fragments arranged by size:
403 339 320
BsmI GAATG_Cn'
Cuts at: 0 199 731 1062
Size: 199 532 331
Fragments arranged by size:
532 331 199
BsrDI GCAATG_nn'
Cuts at: 0 229 332 1062
Size: 229 103 730
Fragments arranged by size:
730 229 103
BssSI C'ACGA_G
Cuts at: 0 700 1062
Size: 700 362
BstAPI GCAn_nnn'nTGC
Cuts at: 0 682 1062
Size: 682 380
BstEII G'GTnAC_C
Cuts at: 0 831 1062
Size: 831 231
BstXI CCAn_nnnn'nTGG
Cuts at: 0 175 1062
Size: 175 887
BstYI r'GATC_y
Cuts at: 0 396 613 1062
Size: 396 217 449
Fragments arranged by size:
449 396 217
BtsI GCAGTG_nn'
Cuts at: 0 636 1062
Size: 636 426
DraI TTT'AAA
Cuts at: 0 573 935 1062
Size: 573 362 127
Fragments arranged by size:
573 362 127
DraIII CAC_nnn'GTG
Cuts at: 0 682 1062
Size: 682 380
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 76 570 1062
Size: 52 24 494 492
Fragments arranged by size:
494 492 52 24
EcoRI G'AATT_C
Cuts at: 0 404 1062
Size: 404 658
EcoRV GAT'ATC
Cuts at: 0 816 1062
Size: 816 246
HindIII A'AGCT_T
Cuts at: 0 64 1062
Size: 64 998
MslI CAynn'nnrTG
Cuts at: 0 173 1062
Size: 173 889
NsiI A_TGCA'T
Cuts at: 0 199 661 731 1062
Size: 199 462 70 331
Fragments arranged by size:
462 331 199 70
NspI r_CATG'y
Cuts at: 0 659 1062
Size: 659 403
PfoI T'CCnGG_A
Cuts at: 0 399 1062
Size: 399 663
ScaI AGT'ACT
Cuts at: 0 141 1062
Size: 141 921
SmlI C'TyrA_G
Cuts at: 0 530 1062
Size: 530 532
StuI AGG'CCT
Cuts at: 0 412 1062
Size: 412 650
StyI C'CwwG_G
Cuts at: 0 270 836 1062
Size: 270 566 226
Fragments arranged by size:
566 270 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1000 1062
Size: 1000 62
TatI w'GTAC_w
Cuts at: 0 139 1062
Size: 139 923
Tth111I GACn'n_nGTC
Cuts at: 0 694 1062
Size: 694 368
XbaI T'CTAG_A
Cuts at: 0 921 1062
Size: 921 141
XhoI C'TCGA_G
Cuts at: 0 530 1062
Size: 530 532
XmnI GAAnn'nnTTC
Cuts at: 0 15 737 1062
Size: 15 722 325
Fragments arranged by size:
722 325 15
Enzymes that do cut:
AcuI AlwNI ApoI AvaI BbsI BbvCI BfrBI BglII
BlpI BmrI BpmI Bpu10I BsgI BsmI BsrDI BssSI
BstAPI BstEII BstXI BstYI BtsI DraI DraIII Eco57MI
EcoRI EcoRV HindIII MslI NsiI NspI PfoI ScaI
SmlI StuI StyI TaqII TatI Tth111I XbaI XhoI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI
BcgI BciVI BclI BglI Bme1580I BmgBI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BstBI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoO109I FalI FseI FspI FspAI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI
XcmI XmaI ZraI