(Linear) (Six-base) MAPSORT of: KIEE3123.seq  Check: 9209  from: 1  to: 930



KIEE3123.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     462     676     930 
   Size:        462     214     254

  Fragments arranged by size: 

                 462     254     214
 
AflIII A'CryG_T

Cuts at:      0     858     930 
   Size:        858      72
 
ApoI r'AATT_y

Cuts at:      0     178     654     930 
   Size:        178     476     276

  Fragments arranged by size: 

                 476     276     178
 
AseI AT'TA_AT

Cuts at:      0     574     930 
   Size:        574     356
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     905     930 
   Size:        905      25
 
BamHI G'GATC_C

Cuts at:      0     435     930 
   Size:        435     495
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     906     930 
   Size:        906      24
 
BclI T'GATC_A

Cuts at:      0     485     930 
   Size:        485     445
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      49     930 
   Size:         49     881
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     930 
   Size:        161     769
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      48      78     167     197     930 
   Size:         48      30      89      30     733

  Fragments arranged by size: 

                 733      89      48      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      38     930 
   Size:         38     892
 
BstBI TT'CG_AA

Cuts at:      0     535     930 
   Size:        535     395
 
BstYI r'GATC_y

Cuts at:      0     435     930 
   Size:        435     495
 
BtsI GCAGTG_nn'

Cuts at:      0      77     431     930 
   Size:         77     354     499

  Fragments arranged by size: 

                 499     354      77
 
DraI TTT'AAA

Cuts at:      0     927     930 
   Size:        927       3
 
EaeI y'GGCC_r

Cuts at:      0     132     930 
   Size:        132     798
 
EarI CTCTTCn'nnn_

Cuts at:      0     105     661     930 
   Size:        105     556     269

  Fragments arranged by size: 

                 556     269     105
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     462     676     930 
   Size:        161     301     214     254

  Fragments arranged by size: 

                 301     254     214     161
 
EcoO109I rG'GnC_Cy

Cuts at:      0     834     930 
   Size:        834      96
 
EcoRV GAT'ATC

Cuts at:      0     378     930 
   Size:        378     552
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     266     389     652     725     890     930 
   Size:        266     123     263      73     165      40

  Fragments arranged by size: 

                 266     263     165     123      73      40
 
MslI CAynn'nnrTG

Cuts at:      0     134     863     930 
   Size:        134     729      67

  Fragments arranged by size: 

                 729     134      67
 
MspA1I CmG'CkG

Cuts at:      0     807     930 
   Size:        807     123
 
NspI r_CATG'y

Cuts at:      0     862     930 
   Size:        862      68
 
PciI A'CATG_T

Cuts at:      0     858     930 
   Size:        858      72
 
PpuMI rG'GwC_Cy

Cuts at:      0     834     930 
   Size:        834      96
 
PstI C_TGCA'G

Cuts at:      0      72     930 
   Size:         72     858
 
PvuII CAG'CTG

Cuts at:      0     807     930 
   Size:        807     123
 
SfcI C'TryA_G

Cuts at:      0      56      68     699     773     930 
   Size:         56      12     631      74     157

  Fragments arranged by size: 

                 631     157      74      56      12
 
SspI AAT'ATT

Cuts at:      0     885     930 
   Size:        885      45
 
TatI w'GTAC_w

Cuts at:      0     867     930 
   Size:        867      63
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     846     930 
   Size:        846      84
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     AseI     BaeI    BamHI    BciVI     BclI
     BmrI     BpmI    BsaXI    BseRI    BstBI    BstYI     BtsI     DraI
     EaeI     EarI  Eco57MI EcoO109I    EcoRV     MmeI     MslI   MspA1I
     NspI     PciI    PpuMI     PstI    PvuII     SfcI     SspI     TatI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
    EcoRI     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI