(Linear) (Six-base) MAPSORT of: KIEE2805.seq  Check: 8221  from: 1  to: 270



KIEE2805.orf

 With 182 enzymes: SgfI * 

                        August 16, 2021 14:16  ..

AflIII A'CryG_T

Cuts at:      0       8     270 
   Size:          8     262
 
AhdI GACnn_n'nnGTC

Cuts at:      0       9     270 
   Size:          9     261
 
BanI G'GyrC_C

Cuts at:      0     205     270 
   Size:        205      65
 
BanII G_rGCy'C

Cuts at:      0      92     270 
   Size:         92     178
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      49     270 
   Size:         49     221
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     270 
   Size:        229      41
 
BseYI C'CCAG_C

Cuts at:      0      65     138     224     270 
   Size:         65      73      86      46

  Fragments arranged by size: 

                  86      73      65      46
 
BsiHKAI G_wGCw'C

Cuts at:      0      92     270 
   Size:         92     178
 
Bsp1286I G_dGCh'C

Cuts at:      0      92     270 
   Size:         92     178
 
BstEII G'GTnAC_C

Cuts at:      0     105     270 
   Size:        105     165
 
BtsI GCAGTG_nn'

Cuts at:      0     237     270 
   Size:        237      33
 
DraIII CAC_nnn'GTG

Cuts at:      0     215     270 
   Size:        215      55
 
EcoICRI GAG'CTC

Cuts at:      0      90     270 
   Size:         90     180
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      27     270 
   Size:         27     243
 
MslI CAynn'nnrTG

Cuts at:      0     164     270 
   Size:        164     106
 
NruI TCG'CGA

Cuts at:      0      94     270 
   Size:         94     176
 
NspI r_CATG'y

Cuts at:      0      12     270 
   Size:         12     258
 
PciI A'CATG_T

Cuts at:      0       8     270 
   Size:          8     262
 
SacI G_AGCT'C

Cuts at:      0      92     270 
   Size:         92     178
 
SmlI C'TyrA_G

Cuts at:      0     244     270 
   Size:        244      26
 
StuI AGG'CCT

Cuts at:      0     127     270 
   Size:        127     143
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      57     270 
   Size:         57     213
 

 Enzymes that do cut:

   AflIII     AhdI     BanI    BanII     BplI    BpuEI    BseYI  BsiHKAI
 Bsp1286I   BstEII     BtsI   DraIII  EcoICRI    Hin4I     MslI     NruI
     NspI     PciI     SacI     SmlI     StuI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI