Restriction map for
pF1KE2770
(Linear) (Six-base) MAPSORT of: KIEE2770.seq Check: 8906 from: 1 to: 3654
KIEE2770.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AccI GT'mk_AC
Cuts at: 0 3074 3654
Size: 3074 580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1054 1120 1285 3654
Size: 1054 66 165 2369
Fragments arranged by size:
2369 1054 165 66
AflII C'TTAA_G
Cuts at: 0 2665 3654
Size: 2665 989
AflIII A'CryG_T
Cuts at: 0 197 3654
Size: 197 3457
AleI CACnn'nnGTG
Cuts at: 0 347 3559 3654
Size: 347 3212 95
Fragments arranged by size:
3212 347 95
AlwNI CAG_nnn'CTG
Cuts at: 0 495 3040 3085 3654
Size: 495 2545 45 569
Fragments arranged by size:
2545 569 495 45
ApaI G_GGCC'C
Cuts at: 0 1615 1740 2155 3654
Size: 1615 125 415 1499
Fragments arranged by size:
1615 1499 415 125
ApaLI G'TGCA_C
Cuts at: 0 3564 3601 3654
Size: 3564 37 53
Fragments arranged by size:
3564 53 37
ApoI r'AATT_y
Cuts at: 0 1260 3100 3535 3654
Size: 1260 1840 435 119
Fragments arranged by size:
1840 1260 435 119
AvaI C'yCGr_G
Cuts at: 0 1733 1859 1922 1973 2350 3654
Size: 1733 126 63 51 377 1304
Fragments arranged by size:
1733 1304 377 126 63 51
BanI G'GyrC_C
Cuts at: 0 1640 3288 3654
Size: 1640 1648 366
Fragments arranged by size:
1648 1640 366
BanII G_rGCy'C
Cuts at: 0 152 1511 1615 1631 1740 1802 2044 2155
Size: 152 1359 104 16 109 62 242 111
Cuts at: 2155 2431 2788 3654
Size: 276 357 866
Fragments arranged by size:
1359 866 357 276 242 152 111 109
104 62 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 1298 1963 3654
Size: 1298 665 1691
Fragments arranged by size:
1691 1298 665
BciVI GTATCCnnnnn_n'
Cuts at: 0 2330 3654
Size: 2330 1324
BfrBI ATG'CAT
Cuts at: 0 3173 3302 3654
Size: 3173 129 352
Fragments arranged by size:
3173 352 129
BglI GCCn_nnn'nGGC
Cuts at: 0 1635 1735 2580 3654
Size: 1635 100 845 1074
Fragments arranged by size:
1635 1074 845 100
BglII A'GATC_T
Cuts at: 0 3090 3654
Size: 3090 564
BlpI GC'TnA_GC
Cuts at: 0 1160 3550 3654
Size: 1160 2390 104
Fragments arranged by size:
2390 1160 104
Bme1580I G_kGCm'C
Cuts at: 0 1615 1740 2012 2155 3568 3605 3654
Size: 1615 125 272 143 1413 37 49
Fragments arranged by size:
1615 1413 272 143 125 49 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 634 3366 3421 3654
Size: 634 2732 55 233
Fragments arranged by size:
2732 634 233 55
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 1704 2001 2527 2562 2772 3654
Size: 584 1120 297 526 35 210 882
Fragments arranged by size:
1120 882 584 526 297 210 35
Bpu10I CC'TnA_GC
Cuts at: 0 1535 2114 2275 3654
Size: 1535 579 161 1379
Fragments arranged by size:
1535 1379 579 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 2424 2870 3654
Size: 782 1642 446 784
Fragments arranged by size:
1642 784 782 446
BsaI GGTCTCn'nnnn_
Cuts at: 0 563 784 1919 3654
Size: 563 221 1135 1735
Fragments arranged by size:
1735 1135 563 221
BsaWI w'CCGG_w
Cuts at: 0 480 1527 2818 3654
Size: 480 1047 1291 836
Fragments arranged by size:
1291 1047 836 480
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1509 1539 2026 2056 3654
Size: 1509 30 487 30 1598
Fragments arranged by size:
1598 1509 487 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1347 1782 2276 2439 2577 2787 3654
Size: 1347 435 494 163 138 210 867
Fragments arranged by size:
1347 867 494 435 210 163 138
BseYI C'CCAG_C
Cuts at: 0 744 821 1521 2342 3654
Size: 744 77 700 821 1312
Fragments arranged by size:
1312 821 744 700 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1349 1445 2645 3654
Size: 1349 96 1200 1009
Fragments arranged by size:
1349 1200 1009 96
BsiHKAI G_wGCw'C
Cuts at: 0 1380 1624 2044 2120 2431 2807 3568 3605
Size: 1380 244 420 76 311 376 761 37
Cuts at: 3605 3654
Size: 49
Fragments arranged by size:
1380 761 420 376 311 244 76 49
37
BsmI GAATG_Cn'
Cuts at: 0 323 1686 3654
Size: 323 1363 1968
Fragments arranged by size:
1968 1363 323
Bsp1286I G_dGCh'C
Cuts at: 0 152 1380 1511 1615 1624 1631 1740 1802
Size: 152 1228 131 104 9 7 109 62
Cuts at: 1802 2012 2044 2120 2155 2431 2788 2807 3568
Size: 210 32 76 35 276 357 19 761
Cuts at: 3568 3605 3654
Size: 37 49
Fragments arranged by size:
1228 761 357 276 210 152 131 109
104 76 62 49 37 35 32 19
9 7
BspEI T'CCGG_A
Cuts at: 0 1527 2818 3654
Size: 1527 1291 836
Fragments arranged by size:
1527 1291 836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 68 1855 2333 3654
Size: 68 1787 478 1321
Fragments arranged by size:
1787 1321 478 68
BsrBI CCG'CTC
Cuts at: 0 2274 3654
Size: 2274 1380
BsrDI GCAATG_nn'
Cuts at: 0 881 2239 3302 3654
Size: 881 1358 1063 352
Fragments arranged by size:
1358 1063 881 352
BsrGI T'GTAC_A
Cuts at: 0 41 3654
Size: 41 3613
BssSI C'ACGA_G
Cuts at: 0 1674 3519 3654
Size: 1674 1845 135
Fragments arranged by size:
1845 1674 135
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3179 3654
Size: 3179 475
BstBI TT'CG_AA
Cuts at: 0 66 3654
Size: 66 3588
BstEII G'GTnAC_C
Cuts at: 0 550 3654
Size: 550 3104
BstYI r'GATC_y
Cuts at: 0 1701 3090 3654
Size: 1701 1389 564
Fragments arranged by size:
1701 1389 564
BtgI C'CryG_G
Cuts at: 0 890 908 1633 2201 3654
Size: 890 18 725 568 1453
Fragments arranged by size:
1453 890 725 568 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 238 3217 3654
Size: 238 2979 437
Fragments arranged by size:
2979 437 238
BtsI GCAGTG_nn'
Cuts at: 0 33 776 955 1428 2012 3393 3405 3654
Size: 33 743 179 473 584 1381 12 249
Fragments arranged by size:
1381 743 584 473 249 179 33 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 9 44 2282 2317 3654
Size: 9 35 2238 35 1337
Fragments arranged by size:
2238 1337 35 35 9
EaeI y'GGCC_r
Cuts at: 0 1120 2450 2697 3654
Size: 1120 1330 247 957
Fragments arranged by size:
1330 1120 957 247
EarI CTCTTCn'nnn_
Cuts at: 0 552 686 1085 2416 2754 2763 3654
Size: 552 134 399 1331 338 9 891
Fragments arranged by size:
1331 891 552 399 338 134 9
EcoICRI GAG'CTC
Cuts at: 0 2042 2429 3654
Size: 2042 387 1225
Fragments arranged by size:
2042 1225 387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 1054 1120 1285 1704 2001 2527 2562
Size: 584 470 66 165 419 297 526 35
Cuts at: 2562 2772 3654
Size: 210 882
Fragments arranged by size:
882 584 526 470 419 297 210 165
66 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 311 499 514 3654
Size: 311 188 15 3140
Fragments arranged by size:
3140 311 188 15
EcoO109I rG'GnC_Cy
Cuts at: 0 762 927 1611 1612 1737 2108 2151 2582
Size: 762 165 684 1 125 371 43 431
Cuts at: 2582 2703 3368 3513 3654
Size: 121 665 145 141
Fragments arranged by size:
762 684 665 431 371 165 145 141
125 121 43 1
EcoRI G'AATT_C
Cuts at: 0 3535 3654
Size: 3535 119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1315 2932 3654
Size: 1315 1617 722
Fragments arranged by size:
1617 1315 722
HaeII r_GCGC'y
Cuts at: 0 3142 3654
Size: 3142 512
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 163 195 489 521 1029 1061 1444 1476
Size: 163 32 294 32 508 32 383 32
Cuts at: 1476 1758 1790 2314 2346 3654
Size: 282 32 524 32 1308
Fragments arranged by size:
1308 524 508 383 294 282 163 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 21 3654
Size: 21 3633
HindIII A'AGCT_T
Cuts at: 0 2298 3654
Size: 2298 1356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 1567 1800 2955 3654
Size: 692 875 233 1155 699
Fragments arranged by size:
1155 875 699 692 233
MscI TGG'CCA
Cuts at: 0 2452 2699 3654
Size: 2452 247 955
Fragments arranged by size:
2452 955 247
MslI CAynn'nnrTG
Cuts at: 0 347 3202 3559 3654
Size: 347 2855 357 95
Fragments arranged by size:
2855 357 347 95
MspA1I CmG'CkG
Cuts at: 0 1159 1563 1597 2171 3654
Size: 1159 404 34 574 1483
Fragments arranged by size:
1483 1159 574 404 34
NcoI C'CATG_G
Cuts at: 0 908 3654
Size: 908 2746
NsiI A_TGCA'T
Cuts at: 0 3175 3304 3654
Size: 3175 129 350
Fragments arranged by size:
3175 350 129
NspI r_CATG'y
Cuts at: 0 201 1204 3177 3654
Size: 201 1003 1973 477
Fragments arranged by size:
1973 1003 477 201
PciI A'CATG_T
Cuts at: 0 197 3654
Size: 197 3457
PflMI CCAn_nnn'nTGG
Cuts at: 0 2202 3654
Size: 2202 1452
PfoI T'CCnGG_A
Cuts at: 0 2210 3654
Size: 2210 1444
PpuMI rG'GwC_Cy
Cuts at: 0 762 2108 2703 3368 3654
Size: 762 1346 595 665 286
Fragments arranged by size:
1346 762 665 595 286
PshAI GACnn'nnGTC
Cuts at: 0 1582 3654
Size: 1582 2072
PspOMI G'GGCC_C
Cuts at: 0 1611 1736 2151 3654
Size: 1611 125 415 1503
Fragments arranged by size:
1611 1503 415 125
PstI C_TGCA'G
Cuts at: 0 1886 3654
Size: 1886 1768
PvuII CAG'CTG
Cuts at: 0 1159 1563 1597 3654
Size: 1159 404 34 2057
Fragments arranged by size:
2057 1159 404 34
SacI G_AGCT'C
Cuts at: 0 2044 2431 3654
Size: 2044 387 1223
Fragments arranged by size:
2044 1223 387
SanDI GG'GwC_CC
Cuts at: 0 3368 3654
Size: 3368 286
SapI GCTCTTCn'nnn_
Cuts at: 0 552 3654
Size: 552 3102
SbfI CC_TGCA'GG
Cuts at: 0 1886 3654
Size: 1886 1768
ScaI AGT'ACT
Cuts at: 0 237 3654
Size: 237 3417
SexAI A'CCwGG_T
Cuts at: 0 3596 3654
Size: 3596 58
SfcI C'TryA_G
Cuts at: 0 1882 3086 3654
Size: 1882 1204 568
Fragments arranged by size:
1882 1204 568
SmaI CCC'GGG
Cuts at: 0 1735 1861 1975 2352 3654
Size: 1735 126 114 377 1302
Fragments arranged by size:
1735 1302 377 126 114
SmlI C'TyrA_G
Cuts at: 0 797 2439 2665 2885 3654
Size: 797 1642 226 220 769
Fragments arranged by size:
1642 797 769 226 220
SpeI A'CTAG_T
Cuts at: 0 346 2182 3654
Size: 346 1836 1472
Fragments arranged by size:
1836 1472 346
SphI G_CATG'C
Cuts at: 0 3177 3654
Size: 3177 477
SrfI GCCC'GGGC
Cuts at: 0 1861 3654
Size: 1861 1793
SspI AAT'ATT
Cuts at: 0 3158 3654
Size: 3158 496
StuI AGG'CCT
Cuts at: 0 1852 3654
Size: 1852 1802
StyI C'CwwG_G
Cuts at: 0 510 758 908 1949 3569 3654
Size: 510 248 150 1041 1620 85
Fragments arranged by size:
1620 1041 510 248 150 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 569 1658 3654
Size: 569 1089 1996
Fragments arranged by size:
1996 1089 569
TatI w'GTAC_w
Cuts at: 0 41 235 1153 3654
Size: 41 194 918 2501
Fragments arranged by size:
2501 918 194 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 346 378 2026 2058 3654
Size: 346 32 1648 32 1596
Fragments arranged by size:
1648 1596 346 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 508 3195 3379 3654
Size: 508 2687 184 275
Fragments arranged by size:
2687 508 275 184
XmaI C'CCGG_G
Cuts at: 0 1733 1859 1973 2350 3654
Size: 1733 126 114 377 1304
Fragments arranged by size:
1733 1304 377 126 114
XmnI GAAnn'nnTTC
Cuts at: 0 131 226 1094 3654
Size: 131 95 868 2560
Fragments arranged by size:
2560 868 131 95
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbsI BciVI BfrBI BglI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI CspCI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII MmeI MscI MslI MspA1I NcoI NsiI
NspI PciI PflMI PfoI PpuMI PshAI PspOMI PstI
PvuII SacI SanDI SapI SbfI ScaI SexAI SfcI
SmaI SmlI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BclI BmgBI BmtI BplI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BspHI BsrFI BssHII BstXI
BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI EciI
EcoRV FseI FspI FspAI HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI RsrII SacII SalI SfiI SfoI SgfI SgrAI
SnaBI SwaI Tth111I XbaI XhoI ZraI