Restriction map for
pF1KE2764
(Linear) (Six-base) MAPSORT of: KIEE2764.seq Check: 9847 from: 1 to: 1572
KIEE2764.orf
With 182 enzymes: SgfI *
July 3, 2020 20:21 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 1572
Size: 250 1322
AflIII A'CryG_T
Cuts at: 0 302 672 1085 1572
Size: 302 370 413 487
Fragments arranged by size:
487 413 370 302
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 111 1168 1192 1342 1548 1572
Size: 111 1057 24 150 206 24
Fragments arranged by size:
1057 206 150 111 24 24
AlwNI CAG_nnn'CTG
Cuts at: 0 843 1572
Size: 843 729
ApaI G_GGCC'C
Cuts at: 0 703 1495 1572
Size: 703 792 77
Fragments arranged by size:
792 703 77
ApoI r'AATT_y
Cuts at: 0 955 1572
Size: 955 617
BamHI G'GATC_C
Cuts at: 0 1410 1572
Size: 1410 162
BanI G'GyrC_C
Cuts at: 0 95 1505 1572
Size: 95 1410 67
Fragments arranged by size:
1410 95 67
BanII G_rGCy'C
Cuts at: 0 85 703 902 1379 1495 1572
Size: 85 618 199 477 116 77
Fragments arranged by size:
618 477 199 116 85 77
BbsI GAAGACnn'nnnn_
Cuts at: 0 115 280 1572
Size: 115 165 1292
Fragments arranged by size:
1292 165 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1524 1558 1572
Size: 1524 34 14
Fragments arranged by size:
1524 34 14
BclI T'GATC_A
Cuts at: 0 1353 1572
Size: 1353 219
BglI GCCn_nnn'nGGC
Cuts at: 0 32 859 1572
Size: 32 827 713
Fragments arranged by size:
827 713 32
BglII A'GATC_T
Cuts at: 0 788 1572
Size: 788 784
Bme1580I G_kGCm'C
Cuts at: 0 703 1495 1572
Size: 703 792 77
Fragments arranged by size:
792 703 77
BmrI ACTGGGnnnn_n'
Cuts at: 0 1524 1572
Size: 1524 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 411 1572
Size: 411 1161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 481 1138 1288 1572
Size: 406 75 657 150 284
Fragments arranged by size:
657 406 284 150 75
Bpu10I CC'TnA_GC
Cuts at: 0 1133 1572
Size: 1133 439
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1327 1572
Size: 1327 245
BsaAI yAC'GTr
Cuts at: 0 1088 1197 1572
Size: 1088 109 375
Fragments arranged by size:
1088 375 109
BsaBI GATnn'nnATC
Cuts at: 0 1390 1572
Size: 1390 182
BsaHI Gr'CG_yC
Cuts at: 0 115 1572
Size: 115 1457
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1081 1111 1572
Size: 1081 30 461
Fragments arranged by size:
1081 461 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 262 265 520 523 697 1135 1572
Size: 262 3 255 3 174 438 437
Fragments arranged by size:
438 437 262 255 174 3 3
BseYI C'CCAG_C
Cuts at: 0 566 921 1572
Size: 566 355 651
Fragments arranged by size:
651 566 355
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1138 1164 1166 1572
Size: 1138 26 2 406
Fragments arranged by size:
1138 406 26 2
BsiHKAI G_wGCw'C
Cuts at: 0 397 1039 1572
Size: 397 642 533
Fragments arranged by size:
642 533 397
BsiWI C'GTAC_G
Cuts at: 0 1088 1572
Size: 1088 484
BsmI GAATG_Cn'
Cuts at: 0 76 602 1572
Size: 76 526 970
Fragments arranged by size:
970 526 76
Bsp1286I G_dGCh'C
Cuts at: 0 85 397 703 902 1039 1379 1495 1572
Size: 85 312 306 199 137 340 116 77
Fragments arranged by size:
340 312 306 199 137 116 85 77
BsrDI GCAATG_nn'
Cuts at: 0 1542 1548 1572
Size: 1542 6 24
Fragments arranged by size:
1542 24 6
BsrFI r'CCGG_y
Cuts at: 0 46 1572
Size: 46 1526
BssSI C'ACGA_G
Cuts at: 0 913 1572
Size: 913 659
BstAPI GCAn_nnn'nTGC
Cuts at: 0 144 1572
Size: 144 1428
BstEII G'GTnAC_C
Cuts at: 0 972 1572
Size: 972 600
BstXI CCAn_nnnn'nTGG
Cuts at: 0 30 1116 1502 1572
Size: 30 1086 386 70
Fragments arranged by size:
1086 386 70 30
BstYI r'GATC_y
Cuts at: 0 254 473 788 1292 1391 1410 1572
Size: 254 219 315 504 99 19 162
Fragments arranged by size:
504 315 254 219 162 99 19
Bsu36I CC'TnA_GG
Cuts at: 0 542 1572
Size: 542 1030
BtsI GCAGTG_nn'
Cuts at: 0 95 137 138 1354 1572
Size: 95 42 1 1216 218
Fragments arranged by size:
1216 218 95 42 1
EaeI y'GGCC_r
Cuts at: 0 20 515 710 1572
Size: 20 495 195 862
Fragments arranged by size:
862 495 195 20
EarI CTCTTCn'nnn_
Cuts at: 0 16 1058 1572
Size: 16 1042 514
Fragments arranged by size:
1042 514 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 406 481 1138 1288 1572
Size: 250 156 75 657 150 284
Fragments arranged by size:
657 284 250 156 150 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 964 1117 1399 1572
Size: 964 153 282 173
Fragments arranged by size:
964 282 173 153
EcoO109I rG'GnC_Cy
Cuts at: 0 700 918 1491 1572
Size: 700 218 573 81
Fragments arranged by size:
700 573 218 81
EcoRI G'AATT_C
Cuts at: 0 955 1572
Size: 955 617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 1040 1572
Size: 143 897 532
Fragments arranged by size:
897 532 143
MscI TGG'CCA
Cuts at: 0 22 517 1572
Size: 22 495 1055
Fragments arranged by size:
1055 495 22
MslI CAynn'nnrTG
Cuts at: 0 607 1572
Size: 607 965
MspA1I CmG'CkG
Cuts at: 0 217 459 1572
Size: 217 242 1113
Fragments arranged by size:
1113 242 217
NaeI GCC'GGC
Cuts at: 0 48 1572
Size: 48 1524
NdeI CA'TA_TG
Cuts at: 0 193 718 1572
Size: 193 525 854
Fragments arranged by size:
854 525 193
NgoMIV G'CCGG_C
Cuts at: 0 46 1572
Size: 46 1526
NspI r_CATG'y
Cuts at: 0 306 676 777 1152 1572
Size: 306 370 101 375 420
Fragments arranged by size:
420 375 370 306 101
PciI A'CATG_T
Cuts at: 0 302 672 1572
Size: 302 370 900
Fragments arranged by size:
900 370 302
PflMI CCAn_nnn'nTGG
Cuts at: 0 663 1077 1572
Size: 663 414 495
Fragments arranged by size:
663 495 414
PfoI T'CCnGG_A
Cuts at: 0 350 1572
Size: 350 1222
PmlI CAC'GTG
Cuts at: 0 1197 1572
Size: 1197 375
PpuMI rG'GwC_Cy
Cuts at: 0 918 1572
Size: 918 654
PshAI GACnn'nnGTC
Cuts at: 0 844 1572
Size: 844 728
PspOMI G'GGCC_C
Cuts at: 0 699 1491 1572
Size: 699 792 81
Fragments arranged by size:
792 699 81
PstI C_TGCA'G
Cuts at: 0 149 1321 1572
Size: 149 1172 251
Fragments arranged by size:
1172 251 149
PvuII CAG'CTG
Cuts at: 0 217 459 1572
Size: 217 242 1113
Fragments arranged by size:
1113 242 217
SfcI C'TryA_G
Cuts at: 0 145 1317 1572
Size: 145 1172 255
Fragments arranged by size:
1172 255 145
SmlI C'TyrA_G
Cuts at: 0 1306 1572
Size: 1306 266
SphI G_CATG'C
Cuts at: 0 1152 1572
Size: 1152 420
StuI AGG'CCT
Cuts at: 0 661 1572
Size: 661 911
StyI C'CwwG_G
Cuts at: 0 57 662 782 1572
Size: 57 605 120 790
Fragments arranged by size:
790 605 120 57
Tth111I GACn'n_nGTC
Cuts at: 0 114 205 1572
Size: 114 91 1367
Fragments arranged by size:
1367 114 91
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 840 1572
Size: 840 732
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaI ApoI BamHI BanI
BanII BbsI BcgI BclI BglI BglII Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsiWI BsmI Bsp1286I BsrDI
BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtsI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI MmeI MscI
MslI MspA1I NaeI NdeI NgoMIV NspI PciI PflMI
PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SfcI
SmlI SphI StuI StyI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbvCI BciVI BfrBI BlpI BmgBI
BmtI BsaI BsaWI BsiEI BsmBI BspEI BspHI BspMI
BsrBI BsrGI BssHII BstBI BstZ17I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI NarI NcoI NheI
NotI NruI NsiI PacI PasI PmeI PpiI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TaqII TatI
TstI XbaI XhoI XmaI XmnI ZraI