(Linear) (Six-base) MAPSORT of: KIEE2763.seq  Check: 6984  from: 1  to: 354



KIEE2763.orf

 With 182 enzymes: SgfI * 

                        October 3, 2019 17:18  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     354 
   Size:        253     101
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     275     354 
   Size:        275      79
 
BanI G'GyrC_C

Cuts at:      0      24     354 
   Size:         24     330
 
BanII G_rGCy'C

Cuts at:      0     217     354 
   Size:        217     137
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      42      78     352     354 
   Size:         42      36     274       2

  Fragments arranged by size: 

                 274      42      36       2
 
BglI GCCn_nnn'nGGC

Cuts at:      0     155     354 
   Size:        155     199
 
Bme1580I G_kGCm'C

Cuts at:      0     133     354 
   Size:        133     221
 
BmgBI CAC'GTC

Cuts at:      0     246     354 
   Size:        246     108
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     311     354 
   Size:        311      43
 
BsaHI Gr'CG_yC

Cuts at:      0      83     354 
   Size:         83     271
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     199     229     309     339     354 
   Size:        199      30      80      30      15

  Fragments arranged by size: 

                 199      80      30      30      15
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     354 
   Size:        175     179
 
Bsp1286I G_dGCh'C

Cuts at:      0     133     217     354 
   Size:        133      84     137

  Fragments arranged by size: 

                 137     133      84
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     197     354 
   Size:        197     157
 
BsrGI T'GTAC_A

Cuts at:      0     224     354 
   Size:        224     130
 
Bsu36I CC'TnA_GG

Cuts at:      0     116     354 
   Size:        116     238
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     311     354 
   Size:        253      58      43

  Fragments arranged by size: 

                 253      58      43
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     115     354 
   Size:        115     239
 
EcoO109I rG'GnC_Cy

Cuts at:      0     147     354 
   Size:        147     207
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     273     354 
   Size:        273      81
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     269     301     354 
   Size:        269      32      53

  Fragments arranged by size: 

                 269      53      32
 
NspI r_CATG'y

Cuts at:      0     102     354 
   Size:        102     252
 
PstI C_TGCA'G

Cuts at:      0      23     354 
   Size:         23     331
 
SfcI C'TryA_G

Cuts at:      0      19     354 
   Size:         19     335
 
SphI G_CATG'C

Cuts at:      0     102     354 
   Size:        102     252
 
TatI w'GTAC_w

Cuts at:      0     224     354 
   Size:        224     130
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     199     231     309     341     354 
   Size:        199      32      78      32      13

  Fragments arranged by size: 

                 199      78      32      32      13
 

 Enzymes that do cut:

     AcuI     AlfI     BanI    BanII     BbsI     BglI Bme1580I    BmgBI
     BpmI    BsaHI    BsaXI     BsgI Bsp1286I    BspMI    BsrGI   Bsu36I
  Eco57MI    EcoNI EcoO109I     FalI    Hin4I     NspI     PstI     SfcI
     SphI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     StyI
     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI