Restriction map for
pF1KE2758
(Linear) (Six-base) MAPSORT of: KIEE2758.seq Check: 9419 from: 1 to: 1962
KIEE2758.orf
With 182 enzymes: SgfI *
August 1, 2019 16:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 329 1962
Size: 28 301 1633
Fragments arranged by size:
1633 301 28
ApaI G_GGCC'C
Cuts at: 0 216 442 1751 1962
Size: 216 226 1309 211
Fragments arranged by size:
1309 226 216 211
ApoI r'AATT_y
Cuts at: 0 755 1962
Size: 755 1207
AvaI C'yCGr_G
Cuts at: 0 787 1962
Size: 787 1175
AvrII C'CTAG_G
Cuts at: 0 487 1962
Size: 487 1475
BanII G_rGCy'C
Cuts at: 0 44 216 442 788 1140 1230 1751 1962
Size: 44 172 226 346 352 90 521 211
Fragments arranged by size:
521 352 346 226 211 172 90 44
BclI T'GATC_A
Cuts at: 0 477 531 1962
Size: 477 54 1431
Fragments arranged by size:
1431 477 54
BglI GCCn_nnn'nGGC
Cuts at: 0 109 211 365 608 1592 1619 1755 1889
Size: 109 102 154 243 984 27 136 134
Cuts at: 1889 1962
Size: 73
Fragments arranged by size:
984 243 154 136 134 109 102 73
27
Bme1580I G_kGCm'C
Cuts at: 0 84 216 442 1751 1962
Size: 84 132 226 1309 211
Fragments arranged by size:
1309 226 211 132 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 356 572 590 818 863 890 1154
Size: 160 196 216 18 228 45 27 264
Cuts at: 1154 1244 1388 1962
Size: 90 144 574
Fragments arranged by size:
574 264 228 216 196 160 144 90
45 27 18
Bpu10I CC'TnA_GC
Cuts at: 0 1229 1962
Size: 1229 733
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 1028 1352 1962
Size: 238 790 324 610
Fragments arranged by size:
790 610 324 238
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 416 1962
Size: 416 1546
BseYI C'CCAG_C
Cuts at: 0 360 1962
Size: 360 1602
BsiHKAI G_wGCw'C
Cuts at: 0 750 1230 1962
Size: 750 480 732
Fragments arranged by size:
750 732 480
BsmI GAATG_Cn'
Cuts at: 0 662 1559 1962
Size: 662 897 403
Fragments arranged by size:
897 662 403
BsmBI CGTCTCn'nnnn_
Cuts at: 0 857 1962
Size: 857 1105
Bsp1286I G_dGCh'C
Cuts at: 0 44 84 216 442 750 788 1140 1230
Size: 44 40 132 226 308 38 352 90
Cuts at: 1230 1751 1962
Size: 521 211
Fragments arranged by size:
521 352 308 226 211 132 90 44
40 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1681 1962
Size: 1681 281
BsrBI CCG'CTC
Cuts at: 0 193 1962
Size: 193 1769
BstEII G'GTnAC_C
Cuts at: 0 565 907 1962
Size: 565 342 1055
Fragments arranged by size:
1055 565 342
BstYI r'GATC_y
Cuts at: 0 46 1399 1962
Size: 46 1353 563
Fragments arranged by size:
1353 563 46
Bsu36I CC'TnA_GG
Cuts at: 0 434 830 1962
Size: 434 396 1132
Fragments arranged by size:
1132 434 396
BtgI C'CryG_G
Cuts at: 0 134 651 1608 1625 1680 1804 1851 1962
Size: 134 517 957 17 55 124 47 111
Fragments arranged by size:
957 517 134 124 111 55 47 17
DraIII CAC_nnn'GTG
Cuts at: 0 1810 1962
Size: 1810 152
DrdI GACnn_nn'nnGTC
Cuts at: 0 428 1241 1962
Size: 428 813 721
Fragments arranged by size:
813 721 428
EaeI y'GGCC_r
Cuts at: 0 131 1962
Size: 131 1831
EarI CTCTTCn'nnn_
Cuts at: 0 393 1107 1962
Size: 393 714 855
Fragments arranged by size:
855 714 393
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 192 1962
Size: 192 1770
EcoICRI GAG'CTC
Cuts at: 0 1228 1962
Size: 1228 734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 160 329 356 572 590 818 863
Size: 28 132 169 27 216 18 228 45
Cuts at: 863 890 1154 1244 1388 1962
Size: 27 264 90 144 574
Fragments arranged by size:
574 264 228 216 169 144 132 90
45 28 27 27 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 559 1112 1317 1962
Size: 559 553 205 645
Fragments arranged by size:
645 559 553 205
EcoO109I rG'GnC_Cy
Cuts at: 0 54 212 213 367 438 439 484 583
Size: 54 158 1 154 71 1 45 99
Cuts at: 583 592 709 718 826 1102 1345 1464 1570
Size: 9 117 9 108 276 243 119 106
Cuts at: 1570 1603 1630 1675 1693 1747 1748 1962
Size: 33 27 45 18 54 1 214
Fragments arranged by size:
276 243 214 158 154 119 117 108
106 99 71 54 54 45 45 33
27 18 9 9 1 1 1
EcoRI G'AATT_C
Cuts at: 0 755 1962
Size: 755 1207
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 63 177 209 696 728 901 933
Size: 31 32 114 32 487 32 173 32
Cuts at: 933 1384 1416 1962
Size: 451 32 546
Fragments arranged by size:
546 487 451 173 114 32 32 32
32 32 31
MfeI C'AATT_G
Cuts at: 0 1918 1962
Size: 1918 44
NcoI C'CATG_G
Cuts at: 0 1608 1625 1680 1962
Size: 1608 17 55 282
Fragments arranged by size:
1608 282 55 17
NdeI CA'TA_TG
Cuts at: 0 311 1962
Size: 311 1651
PfoI T'CCnGG_A
Cuts at: 0 417 588 723 999 1107 1359 1404 1503
Size: 417 171 135 276 108 252 45 99
Cuts at: 1503 1644 1962
Size: 141 318
Fragments arranged by size:
417 318 276 252 171 141 135 108
99 45
PpuMI rG'GwC_Cy
Cuts at: 0 54 484 592 709 826 1345 1464 1570
Size: 54 430 108 117 117 519 119 106
Cuts at: 1570 1603 1630 1675 1693 1962
Size: 33 27 45 18 269
Fragments arranged by size:
519 430 269 119 117 117 108 106
54 45 33 27 18
PspOMI G'GGCC_C
Cuts at: 0 212 438 1747 1962
Size: 212 226 1309 215
Fragments arranged by size:
1309 226 215 212
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1043 1075 1962
Size: 1043 32 887
Fragments arranged by size:
1043 887 32
PstI C_TGCA'G
Cuts at: 0 930 1692 1962
Size: 930 762 270
Fragments arranged by size:
930 762 270
SacI G_AGCT'C
Cuts at: 0 1230 1962
Size: 1230 732
SanDI GG'GwC_CC
Cuts at: 0 1345 1464 1675 1962
Size: 1345 119 211 287
Fragments arranged by size:
1345 287 211 119
SbfI CC_TGCA'GG
Cuts at: 0 1692 1962
Size: 1692 270
SexAI A'CCwGG_T
Cuts at: 0 1026 1062 1248 1599 1962
Size: 1026 36 186 351 363
Fragments arranged by size:
1026 363 351 186 36
SfcI C'TryA_G
Cuts at: 0 926 1688 1962
Size: 926 762 274
Fragments arranged by size:
926 762 274
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 608 1592 1619 1889 1962
Size: 608 984 27 270 73
Fragments arranged by size:
984 608 270 73 27
SmaI CCC'GGG
Cuts at: 0 789 1962
Size: 789 1173
SmlI C'TyrA_G
Cuts at: 0 253 1043 1367 1962
Size: 253 790 324 595
Fragments arranged by size:
790 595 324 253
StyI C'CwwG_G
Cuts at: 0 487 570 884 1237 1608 1625 1680 1962
Size: 487 83 314 353 371 17 55 282
Fragments arranged by size:
487 371 353 314 282 83 55 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 145 1962
Size: 145 1817
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1146 1178 1962
Size: 1146 32 784
Fragments arranged by size:
1146 784 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1615 1962
Size: 1615 347
XmaI C'CCGG_G
Cuts at: 0 787 1962
Size: 787 1175
Enzymes that do cut:
AcuI ApaI ApoI AvaI AvrII BanII BclI BglI
Bme1580I BpmI Bpu10I BpuEI BseRI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BstEII BstYI Bsu36I BtgI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI Hin4I MfeI NcoI NdeI PfoI PpuMI
PspOMI PsrI PstI SacI SanDI SbfI SexAI SfcI
SfiI SmaI SmlI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI
BsiEI BsiWI BspEI BspHI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I BtgZI BtsI ClaI
CspCI DraI EagI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PvuI PvuII RsrII
SacII SalI SapI ScaI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XhoI XmnI ZraI