Restriction map for
pF1KE2757
(Linear) (Six-base) MAPSORT of: KIEE2757.seq Check: 2472 from: 1 to: 5283
KIEE2757.orf
With 182 enzymes: SgfI *
July 18, 2019 20:57 ..
AccI GT'mk_AC
Cuts at: 0 5181 5283
Size: 5181 102
Acc65I G'GTAC_C
Cuts at: 0 3400 5283
Size: 3400 1883
AclI AA'CG_TT
Cuts at: 0 432 3324 5283
Size: 432 2892 1959
Fragments arranged by size:
2892 1959 432
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2693 3850 4439 5283
Size: 2693 1157 589 844
Fragments arranged by size:
2693 1157 844 589
AflIII A'CryG_T
Cuts at: 0 1169 1547 1587 2106 3668 5283
Size: 1169 378 40 519 1562 1615
Fragments arranged by size:
1615 1562 1169 519 378 40
AleI CACnn'nnGTG
Cuts at: 0 2201 5283
Size: 2201 3082
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1739 1771 5283
Size: 1739 32 3512
Fragments arranged by size:
3512 1739 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1019 1621 1759 2669 2790 5283
Size: 1019 602 138 910 121 2493
Fragments arranged by size:
2493 1019 910 602 138 121
ApoI r'AATT_y
Cuts at: 0 1644 2157 2882 3502 3718 3799 4993 5283
Size: 1644 513 725 620 216 81 1194 290
Fragments arranged by size:
1644 1194 725 620 513 290 216 81
AseI AT'TA_AT
Cuts at: 0 3974 3995 5283
Size: 3974 21 1288
Fragments arranged by size:
3974 1288 21
AvaI C'yCGr_G
Cuts at: 0 2241 3485 5283
Size: 2241 1244 1798
Fragments arranged by size:
2241 1798 1244
AvrII C'CTAG_G
Cuts at: 0 3250 5283
Size: 3250 2033
BamHI G'GATC_C
Cuts at: 0 609 2332 5283
Size: 609 1723 2951
Fragments arranged by size:
2951 1723 609
BanI G'GyrC_C
Cuts at: 0 76 1692 2245 3400 5283
Size: 76 1616 553 1155 1883
Fragments arranged by size:
1883 1616 1155 553 76
BanII G_rGCy'C
Cuts at: 0 162 1705 2326 2800 4647 5283
Size: 162 1543 621 474 1847 636
Fragments arranged by size:
1847 1543 636 621 474 162
BbeI G_GCGC'C
Cuts at: 0 1696 5283
Size: 1696 3587
BbsI GAAGACnn'nnnn_
Cuts at: 0 1085 2812 3508 3762 3866 4496 5283
Size: 1085 1727 696 254 104 630 787
Fragments arranged by size:
1727 1085 787 696 630 254 104
BbvCI CC'TCA_GC
Cuts at: 0 1060 2217 4378 5283
Size: 1060 1157 2161 905
Fragments arranged by size:
2161 1157 1060 905
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1989 2023 4486 4497 4520 4531 5283
Size: 1989 34 2463 11 23 11 752
Fragments arranged by size:
2463 1989 752 34 23 11 11
BciVI GTATCCnnnnn_n'
Cuts at: 0 1404 1468 3193 5283
Size: 1404 64 1725 2090
Fragments arranged by size:
2090 1725 1404 64
BclI T'GATC_A
Cuts at: 0 333 2261 3537 5196 5283
Size: 333 1928 1276 1659 87
Fragments arranged by size:
1928 1659 1276 333 87
BfrBI ATG'CAT
Cuts at: 0 3374 5283
Size: 3374 1909
BglI GCCn_nnn'nGGC
Cuts at: 0 2555 5283
Size: 2555 2728
BglII A'GATC_T
Cuts at: 0 3807 5283
Size: 3807 1476
BlpI GC'TnA_GC
Cuts at: 0 151 5283
Size: 151 5132
Bme1580I G_kGCm'C
Cuts at: 0 2509 3486 4157 4199 5283
Size: 2509 977 671 42 1084
Fragments arranged by size:
2509 1084 977 671 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 608 1488 1878 4399 5283
Size: 608 880 390 2521 884
Fragments arranged by size:
2521 884 880 608 390
BmtI G_CTAG'C
Cuts at: 0 815 5283
Size: 815 4468
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3139 5283
Size: 3139 2144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 955 996 1949 4469 4873 4900 5132
Size: 208 747 41 953 2520 404 27 232
Cuts at: 5132 5283
Size: 151
Fragments arranged by size:
2520 953 747 404 232 208 151 41
27
Bpu10I CC'TnA_GC
Cuts at: 0 1060 2217 4378 5283
Size: 1060 1157 2161 905
Fragments arranged by size:
2161 1157 1060 905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 1132 1839 2216 2447 2736 4730 5005
Size: 41 1091 707 377 231 289 1994 275
Cuts at: 5005 5283
Size: 278
Fragments arranged by size:
1994 1091 707 377 289 278 275 231
41
BsaI GGTCTCn'nnnn_
Cuts at: 0 3616 5283
Size: 3616 1667
BsaAI yAC'GTr
Cuts at: 0 1040 5283
Size: 1040 4243
BsaBI GATnn'nnATC
Cuts at: 0 2186 5171 5283
Size: 2186 2985 112
Fragments arranged by size:
2985 2186 112
BsaHI Gr'CG_yC
Cuts at: 0 1693 5283
Size: 1693 3590
BsaWI w'CCGG_w
Cuts at: 0 1444 5283
Size: 1444 3839
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 527 557 578 608 1739 1769 5283
Size: 527 30 21 30 1131 30 3514
Fragments arranged by size:
3514 1131 527 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3909 5283
Size: 3909 1374
BseYI C'CCAG_C
Cuts at: 0 2572 4975 5283
Size: 2572 2403 308
Fragments arranged by size:
2572 2403 308
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1014 4164 5283
Size: 1014 3150 1119
Fragments arranged by size:
3150 1119 1014
BsiEI CG_ry'CG
Cuts at: 0 1116 5283
Size: 1116 4167
BsiHKAI G_wGCw'C
Cuts at: 0 1452 1555 1943 2240 2407 2800 2895 4176
Size: 1452 103 388 297 167 393 95 1281
Cuts at: 4176 5283
Size: 1107
Fragments arranged by size:
1452 1281 1107 393 388 297 167 103
95
BsmI GAATG_Cn'
Cuts at: 0 1175 1468 1582 2857 3374 4254 5283
Size: 1175 293 114 1275 517 880 1029
Fragments arranged by size:
1275 1175 1029 880 517 293 114
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1702 5283
Size: 1702 3581
Bsp1286I G_dGCh'C
Cuts at: 0 162 1452 1555 1705 1943 2240 2326 2407
Size: 162 1290 103 150 238 297 86 81
Cuts at: 2407 2509 2800 2895 3486 4157 4176 4199 4647
Size: 102 291 95 591 671 19 23 448
Cuts at: 4647 5283
Size: 636
Fragments arranged by size:
1290 671 636 591 448 297 291 238
162 150 103 102 95 86 81 23
19
BspHI T'CATG_A
Cuts at: 0 3741 5283
Size: 3741 1542
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3061 3388 5283
Size: 3061 327 1895
Fragments arranged by size:
3061 1895 327
BsrBI CCG'CTC
Cuts at: 0 1146 5283
Size: 1146 4137
BsrDI GCAATG_nn'
Cuts at: 0 425 807 1862 2228 3908 5283
Size: 425 382 1055 366 1680 1375
Fragments arranged by size:
1680 1375 1055 425 382 366
BsrFI r'CCGG_y
Cuts at: 0 1258 5283
Size: 1258 4025
BsrGI T'GTAC_A
Cuts at: 0 2043 5283
Size: 2043 3240
BstAPI GCAn_nnn'nTGC
Cuts at: 0 837 992 3062 3335 3910 5283
Size: 837 155 2070 273 575 1373
Fragments arranged by size:
2070 1373 837 575 273 155
BstXI CCAn_nnnn'nTGG
Cuts at: 0 706 5283
Size: 706 4577
BstYI r'GATC_y
Cuts at: 0 609 1161 2332 3807 4131 5283
Size: 609 552 1171 1475 324 1152
Fragments arranged by size:
1475 1171 1152 609 552 324
Bsu36I CC'TnA_GG
Cuts at: 0 676 1245 5283
Size: 676 569 4038
Fragments arranged by size:
4038 676 569
BtgI C'CryG_G
Cuts at: 0 1500 2514 3092 5283
Size: 1500 1014 578 2191
Fragments arranged by size:
2191 1500 1014 578
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3384 5283
Size: 3384 1899
BtsI GCAGTG_nn'
Cuts at: 0 1107 1452 1576 1941 2451 3336 3797 5283
Size: 1107 345 124 365 510 885 461 1486
Fragments arranged by size:
1486 1107 885 510 461 365 345 124
DraI TTT'AAA
Cuts at: 0 599 5283
Size: 599 4684
DrdI GACnn_nn'nnGTC
Cuts at: 0 1903 1962 2720 4933 5283
Size: 1903 59 758 2213 350
Fragments arranged by size:
2213 1903 758 350 59
EaeI y'GGCC_r
Cuts at: 0 516 804 1256 1260 1596 1716 2529 3462
Size: 516 288 452 4 336 120 813 933
Cuts at: 3462 5283
Size: 1821
Fragments arranged by size:
1821 933 813 516 452 336 288 120
4
EarI CTCTTCn'nnn_
Cuts at: 0 1135 4837 5283
Size: 1135 3702 446
Fragments arranged by size:
3702 1135 446
EcoICRI GAG'CTC
Cuts at: 0 2798 5283
Size: 2798 2485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 955 996 1949 2693 3850 4439 4469
Size: 208 747 41 953 744 1157 589 30
Cuts at: 4469 4873 4900 5132 5283
Size: 404 27 232 151
Fragments arranged by size:
1157 953 747 744 589 404 232 208
151 41 30 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1244 2081 2253 2624 2708 3221 5283
Size: 1244 837 172 371 84 513 2062
Fragments arranged by size:
2062 1244 837 513 371 172 84
EcoO109I rG'GnC_Cy
Cuts at: 0 1140 1482 2227 3254 4213 5283
Size: 1140 342 745 1027 959 1070
Fragments arranged by size:
1140 1070 1027 959 745 342
EcoRI G'AATT_C
Cuts at: 0 3502 5283
Size: 3502 1781
EcoRV GAT'ATC
Cuts at: 0 4101 5283
Size: 4101 1182
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 951 2193 5283
Size: 951 1242 3090
Fragments arranged by size:
3090 1242 951
FseI GG_CCGG'CC
Cuts at: 0 1262 5283
Size: 1262 4021
HaeII r_GCGC'y
Cuts at: 0 1337 1696 2391 5283
Size: 1337 359 695 2892
Fragments arranged by size:
2892 1337 695 359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1814 1846 2389 2421 2614 2646 2734 2766
Size: 1814 32 543 32 193 32 88 32
Cuts at: 2766 2929 2961 3026 3058 3459 3491 3871 3903
Size: 163 32 65 32 401 32 380 32
Cuts at: 3903 5283
Size: 1380
Fragments arranged by size:
1814 1380 543 401 380 193 163 88
65 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1833 5283
Size: 1833 3450
HindIII A'AGCT_T
Cuts at: 0 4651 5283
Size: 4651 632
KasI G'GCGC_C
Cuts at: 0 1692 5283
Size: 1692 3591
KpnI G_GTAC'C
Cuts at: 0 3404 5283
Size: 3404 1879
MfeI C'AATT_G
Cuts at: 0 2640 2814 5283
Size: 2640 174 2469
Fragments arranged by size:
2640 2469 174
MluI A'CGCG_T
Cuts at: 0 1169 5283
Size: 1169 4114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1452 2310 5052 5283
Size: 1452 858 2742 231
Fragments arranged by size:
2742 1452 858 231
MscI TGG'CCA
Cuts at: 0 518 806 1718 5283
Size: 518 288 912 3565
Fragments arranged by size:
3565 912 518 288
MslI CAynn'nnrTG
Cuts at: 0 1222 1499 2105 2201 2269 3433 5283
Size: 1222 277 606 96 68 1164 1850
Fragments arranged by size:
1850 1222 1164 606 277 96 68
MspA1I CmG'CkG
Cuts at: 0 961 998 1019 1064 1118 1894 1993 2339
Size: 961 37 21 45 54 776 99 346
Cuts at: 2339 2537 2576 3443 3461 4377 4675 4776 5283
Size: 198 39 867 18 916 298 101 507
Fragments arranged by size:
961 916 867 776 507 346 298 198
101 99 54 45 39 37 21 18
NaeI GCC'GGC
Cuts at: 0 1260 5283
Size: 1260 4023
NarI GG'CG_CC
Cuts at: 0 1693 5283
Size: 1693 3590
NcoI C'CATG_G
Cuts at: 0 1500 2514 3092 5283
Size: 1500 1014 578 2191
Fragments arranged by size:
2191 1500 1014 578
NdeI CA'TA_TG
Cuts at: 0 231 1437 4583 5283
Size: 231 1206 3146 700
Fragments arranged by size:
3146 1206 700 231
NgoMIV G'CCGG_C
Cuts at: 0 1258 5283
Size: 1258 4025
NheI G'CTAG_C
Cuts at: 0 811 5283
Size: 811 4472
NsiI A_TGCA'T
Cuts at: 0 3376 5283
Size: 3376 1907
NspI r_CATG'y
Cuts at: 0 1591 2110 2274 3149 3438 5283
Size: 1591 519 164 875 289 1845
Fragments arranged by size:
1845 1591 875 519 289 164
PasI CC'CwG_GG
Cuts at: 0 525 1347 1807 2319 5283
Size: 525 822 460 512 2964
Fragments arranged by size:
2964 822 525 512 460
PciI A'CATG_T
Cuts at: 0 1587 2106 5283
Size: 1587 519 3177
Fragments arranged by size:
3177 1587 519
PflMI CCAn_nnn'nTGG
Cuts at: 0 531 1621 1689 1759 1914 5283
Size: 531 1090 68 70 155 3369
Fragments arranged by size:
3369 1090 531 155 70 68
PfoI T'CCnGG_A
Cuts at: 0 861 938 971 4134 5283
Size: 861 77 33 3163 1149
Fragments arranged by size:
3163 1149 861 77 33
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1739 1771 5283
Size: 1739 32 3512
Fragments arranged by size:
3512 1739 32
PpuMI rG'GwC_Cy
Cuts at: 0 1140 2227 3254 4213 5283
Size: 1140 1087 1027 959 1070
Fragments arranged by size:
1140 1087 1070 1027 959
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2027 2059 5283
Size: 2027 32 3224
Fragments arranged by size:
3224 2027 32
PstI C_TGCA'G
Cuts at: 0 966 1123 1893 2167 2344 2953 3058 4817
Size: 966 157 770 274 177 609 105 1759
Cuts at: 4817 4904 5283
Size: 87 379
Fragments arranged by size:
1759 966 770 609 379 274 177 157
105 87
PvuII CAG'CTG
Cuts at: 0 961 998 1894 2339 2576 4377 4675 4776
Size: 961 37 896 445 237 1801 298 101
Cuts at: 4776 5283
Size: 507
Fragments arranged by size:
1801 961 896 507 445 298 237 101
37
SacI G_AGCT'C
Cuts at: 0 2800 5283
Size: 2800 2483
SanDI GG'GwC_CC
Cuts at: 0 1140 4213 5283
Size: 1140 3073 1070
Fragments arranged by size:
3073 1140 1070
SexAI A'CCwGG_T
Cuts at: 0 3021 5283
Size: 3021 2262
SfcI C'TryA_G
Cuts at: 0 962 1119 1134 1889 1960 2163 2340 2949
Size: 962 157 15 755 71 203 177 609
Cuts at: 2949 3054 4813 4900 5045 5277 5283
Size: 105 1759 87 145 232 6
Fragments arranged by size:
1759 962 755 609 232 203 177 157
145 105 87 71 15 6
SfoI GGC'GCC
Cuts at: 0 1694 5283
Size: 1694 3589
SmaI CCC'GGG
Cuts at: 0 2243 3487 5283
Size: 2243 1244 1796
Fragments arranged by size:
2243 1796 1244
SmlI C'TyrA_G
Cuts at: 0 56 1147 1818 2231 2462 2751 4745 4984
Size: 56 1091 671 413 231 289 1994 239
Cuts at: 4984 5283
Size: 299
Fragments arranged by size:
1994 1091 671 413 299 289 239 231
56
SnaBI TAC'GTA
Cuts at: 0 1040 5283
Size: 1040 4243
SpeI A'CTAG_T
Cuts at: 0 3982 5283
Size: 3982 1301
SspI AAT'ATT
Cuts at: 0 472 2050 3940 4109 4692 5210 5283
Size: 472 1578 1890 169 583 518 73
Fragments arranged by size:
1890 1578 583 518 472 169 73
StuI AGG'CCT
Cuts at: 0 3173 5283
Size: 3173 2110
StyI C'CwwG_G
Cuts at: 0 30 705 949 1377 1500 1683 2514 3092
Size: 30 675 244 428 123 183 831 578
Cuts at: 3092 3250 5283
Size: 158 2033
Fragments arranged by size:
2033 831 675 578 428 244 183 158
123 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 23 1493 1572 2037 2459 5283
Size: 23 1470 79 465 422 2824
Fragments arranged by size:
2824 1470 465 422 79 23
TatI w'GTAC_w
Cuts at: 0 2043 5283
Size: 2043 3240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 483 515 2219 2251 5283
Size: 483 32 1704 32 3032
Fragments arranged by size:
3032 1704 483 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2628 3133 5283
Size: 2628 505 2150
Fragments arranged by size:
2628 2150 505
XbaI T'CTAG_A
Cuts at: 0 220 5283
Size: 220 5063
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1344 4972 5283
Size: 1344 3628 311
Fragments arranged by size:
3628 1344 311
XmaI C'CCGG_G
Cuts at: 0 2241 3485 5283
Size: 2241 1244 1798
Fragments arranged by size:
2241 1798 1244
XmnI GAAnn'nnTTC
Cuts at: 0 463 2703 3863 5283
Size: 463 2240 1160 1420
Fragments arranged by size:
2240 1420 1160 463
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApoI AseI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NsiI NspI PasI PciI PflMI PfoI PpiI
PpuMI PsrI PstI PvuII SacI SanDI SexAI SfcI
SfoI SmaI SmlI SnaBI SpeI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI ApaI
ApaLI AscI AsiSI BaeI BmgBI BsiWI BspEI BssHII
BssSI BstBI BstEII BstZ17I ClaI CspCI DraIII EagI
EciI FspI FspAI HpaI NotI NruI PacI PmeI
PmlI PshAI PsiI PspOMI PspXI PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI SgfI SgrAI SphI
SrfI SwaI XhoI ZraI