(Linear) (Six-base) MAPSORT of: KIEE2754.seq  Check: 1608  from: 1  to: 843



KIEE2754.orf

 With 182 enzymes: SgfI * 

                        June 30, 2019 14:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     487     843 
   Size:        487     356
 
Acc65I G'GTAC_C

Cuts at:      0     770     843 
   Size:        770      73
 
AflIII A'CryG_T

Cuts at:      0     540     843 
   Size:        540     303
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     363     843 
   Size:        363     480
 
ApoI r'AATT_y

Cuts at:      0     370     487     843 
   Size:        370     117     356

  Fragments arranged by size: 

                 370     356     117
 
AvrII C'CTAG_G

Cuts at:      0     120     843 
   Size:        120     723
 
BanI G'GyrC_C

Cuts at:      0     770     843 
   Size:        770      73
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      12      46     843 
   Size:         12      34     797

  Fragments arranged by size: 

                 797      34      12
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     843 
   Size:        175     668
 
BsaAI yAC'GTr

Cuts at:      0     343     843 
   Size:        343     500
 
BsaBI GATnn'nnATC

Cuts at:      0      62     843 
   Size:         62     781
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      76     843 
   Size:         76     767
 
BseYI C'CCAG_C

Cuts at:      0     269     843 
   Size:        269     574
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     355     487     843 
   Size:        355     132     356

  Fragments arranged by size: 

                 356     355     132
 
BsrDI GCAATG_nn'

Cuts at:      0     206     843 
   Size:        206     637
 
BsrGI T'GTAC_A

Cuts at:      0     537     843 
   Size:        537     306
 
BstEII G'GTnAC_C

Cuts at:      0     317     843 
   Size:        317     526
 
BtgI C'CryG_G

Cuts at:      0     463     843 
   Size:        463     380
 
FspI TGC'GCA

Cuts at:      0     409     843 
   Size:        409     434
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       4      18      36      50     116     146     148     178 
   Size:          4      14      18      14      66      30       2      30

Cuts at:    178     843 
   Size:        665

  Fragments arranged by size: 

                 665      66      30      30      18      14      14       4
                   2
 
HincII GTy'rAC

Cuts at:      0     306     843 
   Size:        306     537
 
KpnI G_GTAC'C

Cuts at:      0     774     843 
   Size:        774      69
 
MslI CAynn'nnrTG

Cuts at:      0      38     291     724     843 
   Size:         38     253     433     119

  Fragments arranged by size: 

                 433     253     119      38
 
MspA1I CmG'CkG

Cuts at:      0     535     843 
   Size:        535     308
 
NspI r_CATG'y

Cuts at:      0     544     843 
   Size:        544     299
 
PciI A'CATG_T

Cuts at:      0     540     843 
   Size:        540     303
 
PstI C_TGCA'G

Cuts at:      0     498     843 
   Size:        498     345
 
PvuII CAG'CTG

Cuts at:      0     535     843 
   Size:        535     308
 
ScaI AGT'ACT

Cuts at:      0     227     843 
   Size:        227     616
 
SexAI A'CCwGG_T

Cuts at:      0     469     843 
   Size:        469     374
 
SfcI C'TryA_G

Cuts at:      0     494     843 
   Size:        494     349
 
SmlI C'TyrA_G

Cuts at:      0     190     843 
   Size:        190     653
 
SspI AAT'ATT

Cuts at:      0     616     843 
   Size:        616     227
 
StyI C'CwwG_G

Cuts at:      0     120     149     843 
   Size:        120      29     694

  Fragments arranged by size: 

                 694     120      29
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     336     843 
   Size:        336     507
 
TatI w'GTAC_w

Cuts at:      0     225     537     843 
   Size:        225     312     306

  Fragments arranged by size: 

                 312     306     225
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      98     130     843 
   Size:         98      32     713

  Fragments arranged by size: 

                 713      98      32
 

 Enzymes that do cut:

     AarI   Acc65I   AflIII     AlfI     ApoI    AvrII     BanI     BcgI
    BpuEI    BsaAI    BsaBI    BseRI    BseYI    BspMI    BsrDI    BsrGI
   BstEII     BtgI     FspI    Hin4I   HincII     KpnI     MslI   MspA1I
     NspI     PciI     PstI    PvuII     ScaI    SexAI     SfcI     SmlI
     SspI     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI    FspAI    HaeII
  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI