(Linear) (Six-base) MAPSORT of: KIEE2751.seq  Check: 9886  from: 1  to: 816



KIEE2751.orf

 With 182 enzymes: SgfI * 

                        June 30, 2019 14:46  ..

AclI AA'CG_TT

Cuts at:      0     107     816 
   Size:        107     709
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     533     816 
   Size:        533     283
 
AflIII A'CryG_T

Cuts at:      0     493     816 
   Size:        493     323
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     249     281     816 
   Size:        249      32     535

  Fragments arranged by size: 

                 535     249      32
 
ApaLI G'TGCA_C

Cuts at:      0     436     816 
   Size:        436     380
 
ApoI r'AATT_y

Cuts at:      0     324     816 
   Size:        324     492
 
AvaI C'yCGr_G

Cuts at:      0      74     816 
   Size:         74     742
 
BanI G'GyrC_C

Cuts at:      0      83     816 
   Size:         83     733
 
BbeI G_GCGC'C

Cuts at:      0      87     816 
   Size:         87     729
 
BfrBI ATG'CAT

Cuts at:      0      99     816 
   Size:         99     717
 
BglI GCCn_nnn'nGGC

Cuts at:      0     128     816 
   Size:        128     688
 
Bme1580I G_kGCm'C

Cuts at:      0     440     816 
   Size:        440     376
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     244     816 
   Size:        244     572
 
BsaHI Gr'CG_yC

Cuts at:      0      84     816 
   Size:         84     732
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     315     816 
   Size:        315     501
 
BsiHKAI G_wGCw'C

Cuts at:      0     440     816 
   Size:        440     376
 
BsmI GAATG_Cn'

Cuts at:      0     101     816 
   Size:        101     715
 
Bsp1286I G_dGCh'C

Cuts at:      0     440     816 
   Size:        440     376
 
BsrGI T'GTAC_A

Cuts at:      0     283     595     810     816 
   Size:        283     312     215       6

  Fragments arranged by size: 

                 312     283     215       6
 
BstEII G'GTnAC_C

Cuts at:      0     559     816 
   Size:        559     257
 
EarI CTCTTCn'nnn_

Cuts at:      0     237     816 
   Size:        237     579
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     533     816 
   Size:        533     283
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     671     816 
   Size:        671     145
 
EcoRV GAT'ATC

Cuts at:      0     265     816 
   Size:        265     551
 
HaeII r_GCGC'y

Cuts at:      0      87     816 
   Size:         87     729
 
KasI G'GCGC_C

Cuts at:      0      83     816 
   Size:         83     733
 
MfeI C'AATT_G

Cuts at:      0     381     816 
   Size:        381     435
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     377     816 
   Size:        377     439
 
NarI GG'CG_CC

Cuts at:      0      84     816 
   Size:         84     732
 
NsiI A_TGCA'T

Cuts at:      0     101     816 
   Size:        101     715
 
NspI r_CATG'y

Cuts at:      0     143     497     816 
   Size:        143     354     319

  Fragments arranged by size: 

                 354     319     143
 
PciI A'CATG_T

Cuts at:      0     493     816 
   Size:        493     323
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     417     816 
   Size:        417     399
 
SfoI GGC'GCC

Cuts at:      0      85     816 
   Size:         85     731
 
SmaI CCC'GGG

Cuts at:      0      76     816 
   Size:         76     740
 
StyI C'CwwG_G

Cuts at:      0     123     363     463     816 
   Size:        123     240     100     353

  Fragments arranged by size: 

                 353     240     123     100
 
TatI w'GTAC_w

Cuts at:      0     283     595     810     816 
   Size:        283     312     215       6

  Fragments arranged by size: 

                 312     283     215       6
 
XmaI C'CCGG_G

Cuts at:      0      74     816 
   Size:         74     742
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     AloI    ApaLI     ApoI     AvaI     BanI
     BbeI    BfrBI     BglI Bme1580I     BplI    BsaHI     BsgI  BsiHKAI
     BsmI Bsp1286I    BsrGI   BstEII     EarI  Eco57MI    EcoNI    EcoRV
    HaeII     KasI     MfeI     MmeI     NarI     NsiI     NspI     PciI
    PflMI     SfoI     SmaI     StyI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AleI     AlfI    AlwNI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI
    BglII     BlpI    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI EcoO109I    EcoRI     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MluI
     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI