Restriction map for
pF1KE2742
(Linear) (Six-base) MAPSORT of: KIEE2742.seq Check: 1476 from: 1 to: 4083
KIEE2742.orf
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AatII G_ACGT'C
Cuts at: 0 2882 4083
Size: 2882 1201
AccI GT'mk_AC
Cuts at: 0 1127 1694 2882 3572 4083
Size: 1127 567 1188 690 511
Fragments arranged by size:
1188 1127 690 567 511
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 824 1651 2338 3322 3439 3774 4083
Size: 439 385 827 687 984 117 335 309
Fragments arranged by size:
984 827 687 439 385 335 309 117
AgeI A'CCGG_T
Cuts at: 0 614 3132 4083
Size: 614 2518 951
Fragments arranged by size:
2518 951 614
AleI CACnn'nnGTG
Cuts at: 0 509 1252 4083
Size: 509 743 2831
Fragments arranged by size:
2831 743 509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 205 4083
Size: 205 3878
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 554 586 641 673 4083
Size: 554 32 55 32 3410
Fragments arranged by size:
3410 554 55 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 97 1178 1382 1650 2068 3527 4083
Size: 97 1081 204 268 418 1459 556
Fragments arranged by size:
1459 1081 556 418 268 204 97
ApaI G_GGCC'C
Cuts at: 0 2116 2569 2828 4083
Size: 2116 453 259 1255
Fragments arranged by size:
2116 1255 453 259
ApoI r'AATT_y
Cuts at: 0 1025 2167 2613 2630 4083
Size: 1025 1142 446 17 1453
Fragments arranged by size:
1453 1142 1025 446 17
AvaI C'yCGr_G
Cuts at: 0 890 2324 4083
Size: 890 1434 1759
Fragments arranged by size:
1759 1434 890
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1301 1334 1405 1438 4083
Size: 1301 33 71 33 2645
Fragments arranged by size:
2645 1301 71 33 33
BamHI G'GATC_C
Cuts at: 0 402 689 3230 4083
Size: 402 287 2541 853
Fragments arranged by size:
2541 853 402 287
BanI G'GyrC_C
Cuts at: 0 104 675 2945 3259 4083
Size: 104 571 2270 314 824
Fragments arranged by size:
2270 824 571 314 104
BanII G_rGCy'C
Cuts at: 0 121 2116 2569 2828 3245 4083
Size: 121 1995 453 259 417 838
Fragments arranged by size:
1995 838 453 417 259 121
BbsI GAAGACnn'nnnn_
Cuts at: 0 854 3318 4083
Size: 854 2464 765
Fragments arranged by size:
2464 854 765
BbvCI CC'TCA_GC
Cuts at: 0 1111 4083
Size: 1111 2972
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 261 295 2227 2261 4083
Size: 261 34 1932 34 1822
Fragments arranged by size:
1932 1822 261 34 34
BclI T'GATC_A
Cuts at: 0 1796 1949 2210 4083
Size: 1796 153 261 1873
Fragments arranged by size:
1873 1796 261 153
BglI GCCn_nnn'nGGC
Cuts at: 0 748 1221 2573 4083
Size: 748 473 1352 1510
Fragments arranged by size:
1510 1352 748 473
Bme1580I G_kGCm'C
Cuts at: 0 109 336 680 1624 2116 2569 2828 4055
Size: 109 227 344 944 492 453 259 1227
Cuts at: 4055 4083
Size: 28
Fragments arranged by size:
1227 944 492 453 344 259 227 109
28
BmgBI CAC'GTC
Cuts at: 0 966 3576 4083
Size: 966 2610 507
Fragments arranged by size:
2610 966 507
BmrI ACTGGGnnnn_n'
Cuts at: 0 2170 3177 3399 3713 4083
Size: 2170 1007 222 314 370
Fragments arranged by size:
2170 1007 370 314 222
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1126 3259 4083
Size: 1126 2133 824
Fragments arranged by size:
2133 1126 824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1325 2713 2994 3109 3879 4083
Size: 1325 1388 281 115 770 204
Fragments arranged by size:
1388 1325 770 281 204 115
Bpu10I CC'TnA_GC
Cuts at: 0 1111 4083
Size: 1111 2972
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 3931 4083
Size: 472 3459 152
Fragments arranged by size:
3459 472 152
BsaI GGTCTCn'nnnn_
Cuts at: 0 3949 4083
Size: 3949 134
BsaAI yAC'GTr
Cuts at: 0 2103 4083
Size: 2103 1980
BsaHI Gr'CG_yC
Cuts at: 0 2306 2879 3323 3518 4083
Size: 2306 573 444 195 565
Fragments arranged by size:
2306 573 565 444 195
BsaWI w'CCGG_w
Cuts at: 0 614 3132 4083
Size: 614 2518 951
Fragments arranged by size:
2518 951 614
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 554 584 1176 1206 2467 2497 2893 2923
Size: 554 30 592 30 1261 30 396 30
Cuts at: 2923 4083
Size: 1160
Fragments arranged by size:
1261 1160 592 554 396 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 326 1453 1903 4083
Size: 326 1127 450 2180
Fragments arranged by size:
2180 1127 450 326
BseYI C'CCAG_C
Cuts at: 0 10 100 325 548 3070 4083
Size: 10 90 225 223 2522 1013
Fragments arranged by size:
2522 1013 225 223 90 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1411 2652 3513 4083
Size: 766 645 1241 861 570
Fragments arranged by size:
1241 861 766 645 570
BsiEI CG_ry'CG
Cuts at: 0 1167 2263 4083
Size: 1167 1096 1820
Fragments arranged by size:
1820 1167 1096
BsiHKAI G_wGCw'C
Cuts at: 0 2153 2187 3112 4083
Size: 2153 34 925 971
Fragments arranged by size:
2153 971 925 34
BsmI GAATG_Cn'
Cuts at: 0 3967 4083
Size: 3967 116
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3897 4083
Size: 3897 186
Bsp1286I G_dGCh'C
Cuts at: 0 109 121 336 680 1624 2116 2153 2187
Size: 109 12 215 344 944 492 37 34
Cuts at: 2187 2569 2828 3112 3245 4055 4083
Size: 382 259 284 133 810 28
Fragments arranged by size:
944 810 492 382 344 284 259 215
133 109 37 34 28 12
BspHI T'CATG_A
Cuts at: 0 990 4083
Size: 990 3093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 76 1433 3047 4083
Size: 76 1357 1614 1036
Fragments arranged by size:
1614 1357 1036 76
BsrDI GCAATG_nn'
Cuts at: 0 1319 2007 2351 2771 4083
Size: 1319 688 344 420 1312
Fragments arranged by size:
1319 1312 688 420 344
BsrFI r'CCGG_y
Cuts at: 0 614 1684 2331 2417 2699 2767 3132 4083
Size: 614 1070 647 86 282 68 365 951
Fragments arranged by size:
1070 951 647 614 365 282 86 68
BsrGI T'GTAC_A
Cuts at: 0 1287 3011 4083
Size: 1287 1724 1072
Fragments arranged by size:
1724 1287 1072
BssSI C'ACGA_G
Cuts at: 0 3783 4083
Size: 3783 300
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1178 1382 1940 2996 4083
Size: 1178 204 558 1056 1087
Fragments arranged by size:
1178 1087 1056 558 204
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1095 1885 2715 3918 4062 4083
Size: 1095 790 830 1203 144 21
Fragments arranged by size:
1203 1095 830 790 144 21
BstYI r'GATC_y
Cuts at: 0 402 631 689 1378 2065 2558 3230 4083
Size: 402 229 58 689 687 493 672 853
Fragments arranged by size:
853 689 687 672 493 402 229 58
BtgI C'CryG_G
Cuts at: 0 209 558 1776 2120 2373 3817 3904 4083
Size: 209 349 1218 344 253 1444 87 179
Fragments arranged by size:
1444 1218 349 344 253 209 179 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 766 4083
Size: 766 3317
BtsI GCAGTG_nn'
Cuts at: 0 187 710 1830 2997 4083
Size: 187 523 1120 1167 1086
Fragments arranged by size:
1167 1120 1086 523 187
DraIII CAC_nnn'GTG
Cuts at: 0 165 4083
Size: 165 3918
DrdI GACnn_nn'nnGTC
Cuts at: 0 2224 4083
Size: 2224 1859
EaeI y'GGCC_r
Cuts at: 0 144 174 740 1682 2024 2260 2333 2450
Size: 144 30 566 942 342 236 73 117
Cuts at: 2450 4083
Size: 1633
Fragments arranged by size:
1633 942 566 342 236 144 117 73
30
EagI C'GGCC_G
Cuts at: 0 2260 4083
Size: 2260 1823
EarI CTCTTCn'nnn_
Cuts at: 0 34 207 1349 4083
Size: 34 173 1142 2734
Fragments arranged by size:
2734 1142 173 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1480 2713 4083
Size: 1480 1233 1370
Fragments arranged by size:
1480 1370 1233
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 824 1325 1651 2338 2713 2994 3109
Size: 439 385 501 326 687 375 281 115
Cuts at: 3109 3322 3439 3774 3879 4083
Size: 213 117 335 105 204
Fragments arranged by size:
687 501 439 385 375 335 326 281
213 204 117 115 105
EcoNI CCTnn'n_nnAGG
Cuts at: 0 31 3148 4083
Size: 31 3117 935
Fragments arranged by size:
3117 935 31
EcoO109I rG'GnC_Cy
Cuts at: 0 1734 2113 2378 2565 2566 2594 2825 3335
Size: 1734 379 265 187 1 28 231 510
Cuts at: 3335 4083
Size: 748
Fragments arranged by size:
1734 748 510 379 265 231 187 28
1
EcoRI G'AATT_C
Cuts at: 0 2630 4083
Size: 2630 1453
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2523 3081 3612 3678 4083
Size: 2523 558 531 66 405
Fragments arranged by size:
2523 558 531 405 66
HaeII r_GCGC'y
Cuts at: 0 449 2714 3100 4083
Size: 449 2265 386 983
Fragments arranged by size:
2265 983 449 386
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 574 606 636 668 985 1017 2013 2045
Size: 574 32 30 32 317 32 996 32
Cuts at: 2045 2467 2499 2848 2880 3385 3417 3480 3512
Size: 422 32 349 32 505 32 63 32
Cuts at: 3512 4083
Size: 571
Fragments arranged by size:
996 574 571 505 422 349 317 63
32 32 32 32 32 32 32 32
30
HincII GTy'rAC
Cuts at: 0 1107 2883 4083
Size: 1107 1776 1200
Fragments arranged by size:
1776 1200 1107
HindIII A'AGCT_T
Cuts at: 0 3925 4083
Size: 3925 158
HpaI GTT'AAC
Cuts at: 0 1107 4083
Size: 1107 2976
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2216 4083
Size: 2216 1867
MscI TGG'CCA
Cuts at: 0 146 742 2026 2452 4083
Size: 146 596 1284 426 1631
Fragments arranged by size:
1631 1284 596 426 146
MslI CAynn'nnrTG
Cuts at: 0 509 1093 1252 1495 1498 3697 3739 4003
Size: 509 584 159 243 3 2199 42 264
Cuts at: 4003 4060 4083
Size: 57 23
Fragments arranged by size:
2199 584 509 264 243 159 57 42
23 3
MspA1I CmG'CkG
Cuts at: 0 53 1650 2059 2316 2414 2841 3609 4083
Size: 53 1597 409 257 98 427 768 474
Fragments arranged by size:
1597 768 474 427 409 257 98 53
NaeI GCC'GGC
Cuts at: 0 2333 2701 4083
Size: 2333 368 1382
Fragments arranged by size:
2333 1382 368
NcoI C'CATG_G
Cuts at: 0 1776 3817 3904 4083
Size: 1776 2041 87 179
Fragments arranged by size:
2041 1776 179 87
NdeI CA'TA_TG
Cuts at: 0 1637 4083
Size: 1637 2446
NgoMIV G'CCGG_C
Cuts at: 0 2331 2699 4083
Size: 2331 368 1384
Fragments arranged by size:
2331 1384 368
NspI r_CATG'y
Cuts at: 0 325 1461 4083
Size: 325 1136 2622
Fragments arranged by size:
2622 1136 325
PasI CC'CwG_GG
Cuts at: 0 76 1969 4083
Size: 76 1893 2114
Fragments arranged by size:
2114 1893 76
PflMI CCAn_nnn'nTGG
Cuts at: 0 53 953 2018 3167 3630 3744 4083
Size: 53 900 1065 1149 463 114 339
Fragments arranged by size:
1149 1065 900 463 339 114 53
PfoI T'CCnGG_A
Cuts at: 0 478 4083
Size: 478 3605
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 554 586 4083
Size: 554 32 3497
Fragments arranged by size:
3497 554 32
PpuMI rG'GwC_Cy
Cuts at: 0 1734 2594 3335 4083
Size: 1734 860 741 748
Fragments arranged by size:
1734 860 748 741
PshAI GACnn'nnGTC
Cuts at: 0 1880 3322 3941 4083
Size: 1880 1442 619 142
Fragments arranged by size:
1880 1442 619 142
PspOMI G'GGCC_C
Cuts at: 0 2112 2565 2824 4083
Size: 2112 453 259 1259
Fragments arranged by size:
2112 1259 453 259
PstI C_TGCA'G
Cuts at: 0 1378 2064 3149 4083
Size: 1378 686 1085 934
Fragments arranged by size:
1378 1085 934 686
PvuII CAG'CTG
Cuts at: 0 53 1650 2414 2841 3609 4083
Size: 53 1597 764 427 768 474
Fragments arranged by size:
1597 768 764 474 427 53
RsrII CG'GwC_CG
Cuts at: 0 1923 4083
Size: 1923 2160
SalI G'TCGA_C
Cuts at: 0 2881 4083
Size: 2881 1202
ScaI AGT'ACT
Cuts at: 0 1329 4083
Size: 1329 2754
SexAI A'CCwGG_T
Cuts at: 0 1394 4083
Size: 1394 2689
SfcI C'TryA_G
Cuts at: 0 627 1374 1530 2060 2603 3145 4083
Size: 627 747 156 530 543 542 938
Fragments arranged by size:
938 747 627 543 542 530 156
SmaI CCC'GGG
Cuts at: 0 892 2326 4083
Size: 892 1434 1757
Fragments arranged by size:
1757 1434 892
SmlI C'TyrA_G
Cuts at: 0 451 3946 4083
Size: 451 3495 137
Fragments arranged by size:
3495 451 137
SphI G_CATG'C
Cuts at: 0 325 4083
Size: 325 3758
SspI AAT'ATT
Cuts at: 0 928 4083
Size: 928 3155
StuI AGG'CCT
Cuts at: 0 2242 4083
Size: 2242 1841
StyI C'CwwG_G
Cuts at: 0 786 802 1776 2249 3762 3817 3904 4083
Size: 786 16 974 473 1513 55 87 179
Fragments arranged by size:
1513 974 786 473 179 87 55 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 92 521 1064 1734 2963 2984 3127 3993
Size: 92 429 543 670 1229 21 143 866
Cuts at: 3993 4083
Size: 90
Fragments arranged by size:
1229 866 670 543 429 143 92 90
21
TatI w'GTAC_w
Cuts at: 0 422 1287 1327 2129 3011 4083
Size: 422 865 40 802 882 1072
Fragments arranged by size:
1072 882 865 802 422 40
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1176 1208 4083
Size: 1176 32 2875
Fragments arranged by size:
2875 1176 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 90 916 1272 1783 2019 3867 4083
Size: 90 826 356 511 236 1848 216
Fragments arranged by size:
1848 826 511 356 236 216 90
XmaI C'CCGG_G
Cuts at: 0 890 2324 4083
Size: 890 1434 1759
Fragments arranged by size:
1759 1434 890
XmnI GAAnn'nnTTC
Cuts at: 0 392 3064 3682 4083
Size: 392 2672 618 401
Fragments arranged by size:
2672 618 401 392
ZraI GAC'GTC
Cuts at: 0 2880 4083
Size: 2880 1203
Enzymes that do cut:
AatII AccI AcuI AgeI AleI AlfI AloI AlwNI
ApaI ApoI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BclI BglI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstXI
BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NspI PasI PflMI PfoI
PpiI PpuMI PshAI PspOMI PstI PvuII RsrII SalI
ScaI SexAI SfcI SmaI SmlI SphI SspI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AhdI ApaLI
AscI AseI AsiSI AvrII BbeI BciVI BfrBI BglII
BlpI BmtI BsaBI BsiWI BspEI BsrBI BssHII BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI EcoICRI EcoRV
FseI FspI FspAI KasI KpnI MfeI MluI NarI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PsiI PspXI PsrI PvuI SacI SacII SanDI SapI
SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SwaI Tth111I XbaI XhoI