Restriction map for
pF1KE2735
(Linear) (Six-base) MAPSORT of: KIEE2735.seq Check: 8516 from: 1 to: 756
KIEE2735.orf
With 182 enzymes: SgfI *
October 23, 2018 17:35 ..
AatII G_ACGT'C
Cuts at: 0 686 756
Size: 686 70
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 756
Size: 7 749
AlwNI CAG_nnn'CTG
Cuts at: 0 30 756
Size: 30 726
ApaI G_GGCC'C
Cuts at: 0 542 756
Size: 542 214
ApoI r'AATT_y
Cuts at: 0 478 756
Size: 478 278
BanII G_rGCy'C
Cuts at: 0 542 629 756
Size: 542 87 127
Fragments arranged by size:
542 127 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 628 662 756
Size: 628 34 94
Fragments arranged by size:
628 94 34
Bme1580I G_kGCm'C
Cuts at: 0 542 651 756
Size: 542 109 105
Fragments arranged by size:
542 109 105
BmgBI CAC'GTC
Cuts at: 0 461 756
Size: 461 295
BmrI ACTGGGnnnn_n'
Cuts at: 0 363 756
Size: 363 393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 229 298 454 609 756
Size: 228 1 69 156 155 147
Fragments arranged by size:
228 156 155 147 69 1
Bpu10I CC'TnA_GC
Cuts at: 0 292 756
Size: 292 464
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 756
Size: 174 582
BsaAI yAC'GTr
Cuts at: 0 261 756
Size: 261 495
BsaHI Gr'CG_yC
Cuts at: 0 413 683 756
Size: 413 270 73
Fragments arranged by size:
413 270 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 158 188 756
Size: 158 30 568
Fragments arranged by size:
568 158 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 100 484 647 756
Size: 55 45 384 163 109
Fragments arranged by size:
384 163 109 55 45
BseYI C'CCAG_C
Cuts at: 0 74 203 704 756
Size: 74 129 501 52
Fragments arranged by size:
501 129 74 52
BsiEI CG_ry'CG
Cuts at: 0 669 756
Size: 669 87
BsiHKAI G_wGCw'C
Cuts at: 0 133 160 365 756
Size: 133 27 205 391
Fragments arranged by size:
391 205 133 27
BsmBI CGTCTCn'nnnn_
Cuts at: 0 100 565 756
Size: 100 465 191
Fragments arranged by size:
465 191 100
Bsp1286I G_dGCh'C
Cuts at: 0 133 160 365 542 629 651 756
Size: 133 27 205 177 87 22 105
Fragments arranged by size:
205 177 133 105 87 27 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 290 346 756
Size: 290 56 410
Fragments arranged by size:
410 290 56
BsrDI GCAATG_nn'
Cuts at: 0 177 327 756
Size: 177 150 429
Fragments arranged by size:
429 177 150
BsrFI r'CCGG_y
Cuts at: 0 428 756
Size: 428 328
BssSI C'ACGA_G
Cuts at: 0 364 756
Size: 364 392
BstXI CCAn_nnnn'nTGG
Cuts at: 0 322 756
Size: 322 434
BtgI C'CryG_G
Cuts at: 0 621 756
Size: 621 135
DraIII CAC_nnn'GTG
Cuts at: 0 450 756
Size: 450 306
EaeI y'GGCC_r
Cuts at: 0 618 666 756
Size: 618 48 90
Fragments arranged by size:
618 90 48
EagI C'GGCC_G
Cuts at: 0 666 756
Size: 666 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 228 229 298 454 609 756
Size: 7 221 1 69 156 155 147
Fragments arranged by size:
221 156 155 147 69 7 1
EcoO109I rG'GnC_Cy
Cuts at: 0 96 225 335 499 539 695 756
Size: 96 129 110 164 40 156 61
Fragments arranged by size:
164 156 129 110 96 61 40
HaeII r_GCGC'y
Cuts at: 0 313 756
Size: 313 443
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 222 254 756
Size: 222 32 502
Fragments arranged by size:
502 222 32
HincII GTy'rAC
Cuts at: 0 351 756
Size: 351 405
MslI CAynn'nnrTG
Cuts at: 0 514 756
Size: 514 242
MspA1I CmG'CkG
Cuts at: 0 78 207 374 671 704 756
Size: 78 129 167 297 33 52
Fragments arranged by size:
297 167 129 78 52 33
NaeI GCC'GGC
Cuts at: 0 430 756
Size: 430 326
NgoMIV G'CCGG_C
Cuts at: 0 428 756
Size: 428 328
PasI CC'CwG_GG
Cuts at: 0 162 756
Size: 162 594
PfoI T'CCnGG_A
Cuts at: 0 394 756
Size: 394 362
PmlI CAC'GTG
Cuts at: 0 261 756
Size: 261 495
PpuMI rG'GwC_Cy
Cuts at: 0 225 335 756
Size: 225 110 421
Fragments arranged by size:
421 225 110
PspOMI G'GGCC_C
Cuts at: 0 538 756
Size: 538 218
PstI C_TGCA'G
Cuts at: 0 83 756
Size: 83 673
PvuII CAG'CTG
Cuts at: 0 78 207 374 756
Size: 78 129 167 382
Fragments arranged by size:
382 167 129 78
SexAI A'CCwGG_T
Cuts at: 0 446 756
Size: 446 310
SfcI C'TryA_G
Cuts at: 0 79 756
Size: 79 677
SmlI C'TyrA_G
Cuts at: 0 153 756
Size: 153 603
StuI AGG'CCT
Cuts at: 0 403 756
Size: 403 353
StyI C'CwwG_G
Cuts at: 0 5 756
Size: 5 751
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 175 345 540 756
Size: 175 170 195 216
Fragments arranged by size:
216 195 175 170
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 158 190 756
Size: 158 32 566
Fragments arranged by size:
566 158 32
Tth111I GACn'n_nGTC
Cuts at: 0 682 756
Size: 682 74
XmnI GAAnn'nnTTC
Cuts at: 0 268 756
Size: 268 488
ZraI GAC'GTC
Cuts at: 0 684 756
Size: 684 72
Enzymes that do cut:
AatII AcuI AlwNI ApaI ApoI BanII BcgI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI
BsrFI BssSI BstXI BtgI DraIII EaeI EagI Eco57MI
EcoO109I HaeII Hin4I HincII MslI MspA1I NaeI NgoMIV
PasI PfoI PmlI PpuMI PspOMI PstI PvuII SexAI
SfcI SmlI StuI StyI TaqII TstI Tth111I XmnI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI BmtI BplI
BsaI BsaBI BsaWI BsgI BsiWI BsmI BspEI BspHI
BsrBI BsrGI BssHII BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI XbaI
XcmI XhoI XmaI