(Linear) (Six-base) MAPSORT of: KIEE2730.seq  Check: 3965  from: 1  to: 657



KIEE2730.orf

 With 182 enzymes: SgfI * 

                        July 14, 2018 20:54  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     139     657 
   Size:        139     518
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     464     657 
   Size:        464     193
 
ApoI r'AATT_y

Cuts at:      0     173     657 
   Size:        173     484
 
BanI G'GyrC_C

Cuts at:      0      69     127     657 
   Size:         69      58     530

  Fragments arranged by size: 

                 530      69      58
 
BanII G_rGCy'C

Cuts at:      0     635     657 
   Size:        635      22
 
BclI T'GATC_A

Cuts at:      0      41     657 
   Size:         41     616
 
BfrBI ATG'CAT

Cuts at:      0     603     657 
   Size:        603      54
 
Bme1580I G_kGCm'C

Cuts at:      0     395     657 
   Size:        395     262
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     363     478     657 
   Size:        363     115     179

  Fragments arranged by size: 

                 363     179     115
 
Bpu10I CC'TnA_GC

Cuts at:      0     325     657 
   Size:        325     332
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     619     657 
   Size:        619      38
 
BsaAI yAC'GTr

Cuts at:      0      61     657 
   Size:         61     596
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     563     657 
   Size:        563      94
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     423     657 
   Size:        423     234
 
BsiHKAI G_wGCw'C

Cuts at:      0     635     657 
   Size:        635      22
 
Bsp1286I G_dGCh'C

Cuts at:      0     395     635     657 
   Size:        395     240      22

  Fragments arranged by size: 

                 395     240      22
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     139     657 
   Size:        139     518
 
BssSI C'ACGA_G

Cuts at:      0     545     657 
   Size:        545     112
 
BstBI TT'CG_AA

Cuts at:      0     144     536     657 
   Size:        144     392     121

  Fragments arranged by size: 

                 392     144     121
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     513     657 
   Size:        513     144
 
DraI TTT'AAA

Cuts at:      0     426     657 
   Size:        426     231
 
EcoICRI GAG'CTC

Cuts at:      0     633     657 
   Size:        633      24
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     363     464     478     657 
   Size:        363     101      14     179

  Fragments arranged by size: 

                 363     179     101      14
 
MslI CAynn'nnrTG

Cuts at:      0     480     657 
   Size:        480     177
 
MspA1I CmG'CkG

Cuts at:      0     118     456     657 
   Size:        118     338     201

  Fragments arranged by size: 

                 338     201     118
 
NsiI A_TGCA'T

Cuts at:      0     605     657 
   Size:        605      52
 
NspI r_CATG'y

Cuts at:      0     324     436     603     657 
   Size:        324     112     167      54

  Fragments arranged by size: 

                 324     167     112      54
 
PfoI T'CCnGG_A

Cuts at:      0     379     657 
   Size:        379     278
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     607     639     657 
   Size:        607      32      18

  Fragments arranged by size: 

                 607      32      18
 
PvuII CAG'CTG

Cuts at:      0     118     456     657 
   Size:        118     338     201

  Fragments arranged by size: 

                 338     201     118
 
SacI G_AGCT'C

Cuts at:      0     635     657 
   Size:        635      22
 
SexAI A'CCwGG_T

Cuts at:      0     464     657 
   Size:        464     193
 
SmlI C'TyrA_G

Cuts at:      0     634     657 
   Size:        634      23
 
SnaBI TAC'GTA

Cuts at:      0      61     657 
   Size:         61     596
 
SphI G_CATG'C

Cuts at:      0     436     657 
   Size:        436     221
 
XmnI GAAnn'nnTTC

Cuts at:      0     173     657 
   Size:        173     484
 

 Enzymes that do cut:

     AarI     AcuI     ApoI     BanI    BanII     BclI    BfrBI Bme1580I
     BpmI   Bpu10I    BpuEI    BsaAI    BseRI     BsgI  BsiHKAI Bsp1286I
    BspMI    BssSI    BstBI    BtgZI     DraI  EcoICRI  Eco57MI     MslI
   MspA1I     NsiI     NspI     PfoI     PpiI    PvuII     SacI    SexAI
     SmlI    SnaBI     SphI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI    PflMI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SpeI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI