Restriction map for
pF1KE2729
(Linear) (Six-base) MAPSORT of: KIEE2729.seq Check: 1534 from: 1 to: 1395
KIEE2729.orf
With 182 enzymes: SgfI *
October 2, 2018 18:08 ..
AfeI AGC'GCT
Cuts at: 0 841 1395
Size: 841 554
ApaI G_GGCC'C
Cuts at: 0 1347 1395
Size: 1347 48
AvrII C'CTAG_G
Cuts at: 0 513 1395
Size: 513 882
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 717 750 1395
Size: 717 33 645
Fragments arranged by size:
717 645 33
BanI G'GyrC_C
Cuts at: 0 193 531 1395
Size: 193 338 864
Fragments arranged by size:
864 338 193
BanII G_rGCy'C
Cuts at: 0 545 571 1347 1381 1395
Size: 545 26 776 34 14
Fragments arranged by size:
776 545 34 26 14
BbeI G_GCGC'C
Cuts at: 0 535 1395
Size: 535 860
BbsI GAAGACnn'nnnn_
Cuts at: 0 801 1376 1395
Size: 801 575 19
Fragments arranged by size:
801 575 19
BbvCI CC'TCA_GC
Cuts at: 0 588 1208 1395
Size: 588 620 187
Fragments arranged by size:
620 588 187
BglI GCCn_nnn'nGGC
Cuts at: 0 540 1395
Size: 540 855
Bme1580I G_kGCm'C
Cuts at: 0 257 1023 1347 1395
Size: 257 766 324 48
Fragments arranged by size:
766 324 257 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 1026 1395
Size: 1026 369
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 555 647 662 1014 1118 1150 1395
Size: 540 15 92 15 352 104 32 245
Fragments arranged by size:
540 352 245 104 92 32 15 15
Bpu10I CC'TnA_GC
Cuts at: 0 588 1208 1395
Size: 588 620 187
Fragments arranged by size:
620 588 187
BsaHI Gr'CG_yC
Cuts at: 0 532 1395
Size: 532 863
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 277 307 1036 1066 1395
Size: 277 30 729 30 329
Fragments arranged by size:
729 329 277 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 93 1279 1395
Size: 93 1186 116
Fragments arranged by size:
1186 116 93
BseYI C'CCAG_C
Cuts at: 0 604 929 1395
Size: 604 325 466
Fragments arranged by size:
604 466 325
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1141 1395
Size: 1141 254
BsmBI CGTCTCn'nnnn_
Cuts at: 0 280 1332 1395
Size: 280 1052 63
Fragments arranged by size:
1052 280 63
Bsp1286I G_dGCh'C
Cuts at: 0 257 545 571 1023 1347 1381 1395
Size: 257 288 26 452 324 34 14
Fragments arranged by size:
452 324 288 257 34 26 14
BsrDI GCAATG_nn'
Cuts at: 0 617 1395
Size: 617 778
BsrGI T'GTAC_A
Cuts at: 0 140 1395
Size: 140 1255
BstAPI GCAn_nnn'nTGC
Cuts at: 0 956 1395
Size: 956 439
BstXI CCAn_nnnn'nTGG
Cuts at: 0 447 604 1062 1395
Size: 447 157 458 333
Fragments arranged by size:
458 447 333 157
Bsu36I CC'TnA_GG
Cuts at: 0 794 1395
Size: 794 601
BtgI C'CryG_G
Cuts at: 0 1114 1362 1395
Size: 1114 248 33
Fragments arranged by size:
1114 248 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 45 1200 1395
Size: 45 1155 195
Fragments arranged by size:
1155 195 45
BtsI GCAGTG_nn'
Cuts at: 0 545 714 909 1395
Size: 545 169 195 486
Fragments arranged by size:
545 486 195 169
EarI CTCTTCn'nnn_
Cuts at: 0 1204 1241 1395
Size: 1204 37 154
Fragments arranged by size:
1204 154 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 555 647 662 1014 1118 1150 1395
Size: 540 15 92 15 352 104 32 245
Fragments arranged by size:
540 352 245 104 92 32 15 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1351 1395
Size: 1351 44
EcoO109I rG'GnC_Cy
Cuts at: 0 1344 1395
Size: 1344 51
HaeII r_GCGC'y
Cuts at: 0 535 843 1094 1395
Size: 535 308 251 301
Fragments arranged by size:
535 308 301 251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 279 311 377 409 484 516 1036 1068
Size: 279 32 66 32 75 32 520 32
Cuts at: 1068 1395
Size: 327
Fragments arranged by size:
520 327 279 75 66 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1303 1395
Size: 1303 92
KasI G'GCGC_C
Cuts at: 0 531 1395
Size: 531 864
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 1395
Size: 241 1154
MslI CAynn'nnrTG
Cuts at: 0 425 1395
Size: 425 970
MspA1I CmG'CkG
Cuts at: 0 1207 1395
Size: 1207 188
NarI GG'CG_CC
Cuts at: 0 532 1395
Size: 532 863
NcoI C'CATG_G
Cuts at: 0 1114 1395
Size: 1114 281
PasI CC'CwG_GG
Cuts at: 0 564 1395
Size: 564 831
PfoI T'CCnGG_A
Cuts at: 0 983 1395
Size: 983 412
PspOMI G'GGCC_C
Cuts at: 0 1343 1395
Size: 1343 52
PstI C_TGCA'G
Cuts at: 0 552 780 952 1109 1395
Size: 552 228 172 157 286
Fragments arranged by size:
552 286 228 172 157
PvuII CAG'CTG
Cuts at: 0 1207 1395
Size: 1207 188
SapI GCTCTTCn'nnn_
Cuts at: 0 1204 1395
Size: 1204 191
SfcI C'TryA_G
Cuts at: 0 29 205 548 611 776 948 1105 1395
Size: 29 176 343 63 165 172 157 290
Fragments arranged by size:
343 290 176 172 165 157 63 29
SfoI GGC'GCC
Cuts at: 0 533 1395
Size: 533 862
StyI C'CwwG_G
Cuts at: 0 513 1114 1395
Size: 513 601 281
Fragments arranged by size:
601 513 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1107 1127 1395
Size: 1107 20 268
Fragments arranged by size:
1107 268 20
TatI w'GTAC_w
Cuts at: 0 140 1395
Size: 140 1255
Tth111I GACn'n_nGTC
Cuts at: 0 496 1395
Size: 496 899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1360 1395
Size: 1360 35
XmnI GAAnn'nnTTC
Cuts at: 0 53 1395
Size: 53 1342
Enzymes that do cut:
AfeI ApaI AvrII BaeI BanI BanII BbeI BbsI
BbvCI BglI Bme1580I BmrI BpmI Bpu10I BsaHI BsaXI
BseRI BseYI BsgI BsmBI Bsp1286I BsrDI BsrGI BstAPI
BstXI Bsu36I BtgI BtgZI BtsI EarI Eco57MI EcoNI
EcoO109I HaeII Hin4I HindIII KasI MmeI MslI MspA1I
NarI NcoI PasI PfoI PspOMI PstI PvuII SapI
SfcI SfoI StyI TaqII TatI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaWI BsiEI BsiHKAI BsiWI BsmI BspEI
BspHI BspMI BsrBI BsrFI BssHII BssSI BstBI BstEII
BstYI BstZ17I ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HpaI KpnI MfeI MluI MscI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
XbaI XhoI XmaI ZraI