Restriction map for
pF1KE2719
(Linear) (Six-base) MAPSORT of: KIEE2719.seq Check: 5197 from: 1 to: 7770
KIEE2719.orf
With 182 enzymes: SgfI *
February 25, 2018 13:08 ..
AccI GT'mk_AC
Cuts at: 0 503 1398 4581 7770
Size: 503 895 3183 3189
Fragments arranged by size:
3189 3183 895 503
Acc65I G'GTAC_C
Cuts at: 0 5837 7770
Size: 5837 1933
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 1178 1507 4464 4526 5315 5401 6625
Size: 166 1012 329 2957 62 789 86 1224
Cuts at: 6625 7770
Size: 1145
Fragments arranged by size:
2957 1224 1145 1012 789 329 166 86
62
AflII C'TTAA_G
Cuts at: 0 2199 4174 7770
Size: 2199 1975 3596
Fragments arranged by size:
3596 2199 1975
AleI CACnn'nnGTG
Cuts at: 0 3454 4808 5695 7770
Size: 3454 1354 887 2075
Fragments arranged by size:
3454 2075 1354 887
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 183 1570 1673 7770
Size: 183 1387 103 6097
Fragments arranged by size:
6097 1387 183 103
AlwNI CAG_nnn'CTG
Cuts at: 0 184 477 2266 3213 3585 4435 7117 7432
Size: 184 293 1789 947 372 850 2682 315
Cuts at: 7432 7770
Size: 338
Fragments arranged by size:
2682 1789 947 850 372 338 315 293
184
ApoI r'AATT_y
Cuts at: 0 327 897 934 1350 1706 1777 3084 3268
Size: 327 570 37 416 356 71 1307 184
Cuts at: 3268 3276 3849 5075 5645 5915 7770
Size: 8 573 1226 570 270 1855
Fragments arranged by size:
1855 1307 1226 573 570 570 416 356
327 270 184 71 37 8
AseI AT'TA_AT
Cuts at: 0 7268 7770
Size: 7268 502
AvaI C'yCGr_G
Cuts at: 0 60 1073 7770
Size: 60 1013 6697
Fragments arranged by size:
6697 1013 60
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4739 4772 7770
Size: 4739 33 2998
Fragments arranged by size:
4739 2998 33
BanI G'GyrC_C
Cuts at: 0 3075 5837 7735 7770
Size: 3075 2762 1898 35
Fragments arranged by size:
3075 2762 1898 35
BanII G_rGCy'C
Cuts at: 0 121 944 7770
Size: 121 823 6826
Fragments arranged by size:
6826 823 121
BbeI G_GCGC'C
Cuts at: 0 7739 7770
Size: 7739 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 367 3242 4417 4595 6528 7770
Size: 367 2875 1175 178 1933 1242
Fragments arranged by size:
2875 1933 1242 1175 367 178
BbvCI CC'TCA_GC
Cuts at: 0 1103 6401 7770
Size: 1103 5298 1369
Fragments arranged by size:
5298 1369 1103
BciVI GTATCCnnnnn_n'
Cuts at: 0 2406 3966 4956 7770
Size: 2406 1560 990 2814
Fragments arranged by size:
2814 2406 1560 990
BclI T'GATC_A
Cuts at: 0 590 4812 7770
Size: 590 4222 2958
Fragments arranged by size:
4222 2958 590
BfrBI ATG'CAT
Cuts at: 0 1650 7770
Size: 1650 6120
BglI GCCn_nnn'nGGC
Cuts at: 0 2530 7770
Size: 2530 5240
BglII A'GATC_T
Cuts at: 0 747 7770
Size: 747 7023
BlpI GC'TnA_GC
Cuts at: 0 115 7391 7770
Size: 115 7276 379
Fragments arranged by size:
7276 379 115
Bme1580I G_kGCm'C
Cuts at: 0 2526 2706 7770
Size: 2526 180 5064
Fragments arranged by size:
5064 2526 180
BmgBI CAC'GTC
Cuts at: 0 1363 7770
Size: 1363 6407
BmrI ACTGGGnnnn_n'
Cuts at: 0 87 228 3788 4871 7770
Size: 87 141 3560 1083 2899
Fragments arranged by size:
3560 2899 1083 141 87
BmtI G_CTAG'C
Cuts at: 0 5633 7770
Size: 5633 2137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 1627 1856 3200 3836 4217 5432 7082
Size: 454 1173 229 1344 636 381 1215 1650
Cuts at: 7082 7770
Size: 688
Fragments arranged by size:
1650 1344 1215 1173 688 636 454 381
229
Bpu10I CC'TnA_GC
Cuts at: 0 1103 6401 6511 7770
Size: 1103 5298 110 1259
Fragments arranged by size:
5298 1259 1103 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 5439 6794 7770
Size: 5439 1355 976
Fragments arranged by size:
5439 1355 976
BsaI GGTCTCn'nnnn_
Cuts at: 0 1309 5203 6064 6334 7471 7770
Size: 1309 3894 861 270 1137 299
Fragments arranged by size:
3894 1309 1137 861 299 270
BsaBI GATnn'nnATC
Cuts at: 0 1997 4490 7770
Size: 1997 2493 3280
Fragments arranged by size:
3280 2493 1997
BsaHI Gr'CG_yC
Cuts at: 0 7736 7770
Size: 7736 34
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1562 1592 3470 3500 5518 5548 7055 7085
Size: 1562 30 1878 30 2018 30 1507 30
Cuts at: 7085 7770
Size: 685
Fragments arranged by size:
2018 1878 1562 1507 685 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1282 3491 4838 6913 7604 7770
Size: 1282 2209 1347 2075 691 166
Fragments arranged by size:
2209 2075 1347 1282 691 166
BseYI C'CCAG_C
Cuts at: 0 355 660 5717 6516 7742 7770
Size: 355 305 5057 799 1226 28
Fragments arranged by size:
5057 1226 799 355 305 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 549 1343 1650 5089 7770
Size: 549 794 307 3439 2681
Fragments arranged by size:
3439 2681 794 549 307
BsiHKAI G_wGCw'C
Cuts at: 0 4802 7770
Size: 4802 2968
BsmI GAATG_Cn'
Cuts at: 0 172 840 1132 2850 5766 6117 6926 7770
Size: 172 668 292 1718 2916 351 809 844
Fragments arranged by size:
2916 1718 844 809 668 351 292 172
BsmBI CGTCTCn'nnnn_
Cuts at: 0 988 7045 7301 7770
Size: 988 6057 256 469
Fragments arranged by size:
6057 988 469 256
Bsp1286I G_dGCh'C
Cuts at: 0 121 944 2526 2706 4802 7770
Size: 121 823 1582 180 2096 2968
Fragments arranged by size:
2968 2096 1582 823 180 121
BspHI T'CATG_A
Cuts at: 0 3600 5139 6042 7277 7770
Size: 3600 1539 903 1235 493
Fragments arranged by size:
3600 1539 1235 903 493
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1243 1365 1910 3593 7770
Size: 1243 122 545 1683 4177
Fragments arranged by size:
4177 1683 1243 545 122
BsrDI GCAATG_nn'
Cuts at: 0 1067 1919 2695 7082 7770
Size: 1067 852 776 4387 688
Fragments arranged by size:
4387 1067 852 776 688
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2461 7770
Size: 2461 5309
BstBI TT'CG_AA
Cuts at: 0 1377 7770
Size: 1377 6393
BstXI CCAn_nnnn'nTGG
Cuts at: 0 259 2304 3456 5559 7770
Size: 259 2045 1152 2103 2211
Fragments arranged by size:
2211 2103 2045 1152 259
BstYI r'GATC_y
Cuts at: 0 621 747 3418 5805 5961 5988 7010 7770
Size: 621 126 2671 2387 156 27 1022 760
Fragments arranged by size:
2671 2387 1022 760 621 156 126 27
Bsu36I CC'TnA_GG
Cuts at: 0 5639 5966 7770
Size: 5639 327 1804
Fragments arranged by size:
5639 1804 327
BtgI C'CryG_G
Cuts at: 0 1228 6836 7503 7770
Size: 1228 5608 667 267
Fragments arranged by size:
5608 1228 667 267
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 6381 7770
Size: 6381 1389
BtsI GCAGTG_nn'
Cuts at: 0 87 2540 4209 5746 6117 6707 7770
Size: 87 2453 1669 1537 371 590 1063
Fragments arranged by size:
2453 1669 1537 1063 590 371 87
ClaI AT'CG_AT
Cuts at: 0 2001 7770
Size: 2001 5769
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2459 2494 7770
Size: 2459 35 5276
Fragments arranged by size:
5276 2459 35
DraI TTT'AAA
Cuts at: 0 4289 6551 7770
Size: 4289 2262 1219
Fragments arranged by size:
4289 2262 1219
DraIII CAC_nnn'GTG
Cuts at: 0 1366 7770
Size: 1366 6404
EaeI y'GGCC_r
Cuts at: 0 1847 2138 6363 7770
Size: 1847 291 4225 1407
Fragments arranged by size:
4225 1847 1407 291
EarI CTCTTCn'nnn_
Cuts at: 0 549 918 2063 2540 4060 6017 6480 7770
Size: 549 369 1145 477 1520 1957 463 1290
Fragments arranged by size:
1957 1520 1290 1145 549 477 463 369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 454 1178 1507 1627 1856 3200 3836
Size: 166 288 724 329 120 229 1344 636
Cuts at: 3836 4217 4464 4526 5315 5401 5432 6625 7082
Size: 381 247 62 789 86 31 1193 457
Cuts at: 7082 7770
Size: 688
Fragments arranged by size:
1344 1193 789 724 688 636 457 381
329 288 247 229 166 120 86 62
31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 604 6578 6962 7770
Size: 604 5974 384 808
Fragments arranged by size:
5974 808 604 384
EcoRI G'AATT_C
Cuts at: 0 1706 1777 7770
Size: 1706 71 5993
Fragments arranged by size:
5993 1706 71
EcoRV GAT'ATC
Cuts at: 0 1065 1278 4224 7770
Size: 1065 213 2946 3546
Fragments arranged by size:
3546 2946 1065 213
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4552 5834 6821 6847 7770
Size: 4552 1282 987 26 923
Fragments arranged by size:
4552 1282 987 923 26
HaeII r_GCGC'y
Cuts at: 0 7739 7770
Size: 7739 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 1419 1451 2112 2144 3964 3996
Size: 43 32 1344 32 661 32 1820 32
Cuts at: 3996 4930 4962 5134 5166 5537 5569 7002 7034
Size: 934 32 172 32 371 32 1433 32
Cuts at: 7034 7099 7131 7770
Size: 65 32 639
Fragments arranged by size:
1820 1433 1344 934 661 639 371 172
65 43 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1399 4643 5444 7458 7770
Size: 1399 3244 801 2014 312
Fragments arranged by size:
3244 2014 1399 801 312
HindIII A'AGCT_T
Cuts at: 0 416 3721 4703 5406 7770
Size: 416 3305 982 703 2364
Fragments arranged by size:
3305 2364 982 703 416
HpaI GTT'AAC
Cuts at: 0 5444 7458 7770
Size: 5444 2014 312
Fragments arranged by size:
5444 2014 312
KasI G'GCGC_C
Cuts at: 0 7735 7770
Size: 7735 35
KpnI G_GTAC'C
Cuts at: 0 5841 7770
Size: 5841 1929
MfeI C'AATT_G
Cuts at: 0 569 3705 6335 7770
Size: 569 3136 2630 1435
Fragments arranged by size:
3136 2630 1435 569
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 873 1855 2321 4101 5245 5682 7770
Size: 873 982 466 1780 1144 437 2088
Fragments arranged by size:
2088 1780 1144 982 873 466 437
MscI TGG'CCA
Cuts at: 0 2140 6365 7770
Size: 2140 4225 1405
Fragments arranged by size:
4225 2140 1405
MslI CAynn'nnrTG
Cuts at: 0 1655 3454 4565 4808 4887 5695 7048 7770
Size: 1655 1799 1111 243 79 808 1353 722
Fragments arranged by size:
1799 1655 1353 1111 808 722 243 79
MspA1I CmG'CkG
Cuts at: 0 157 1550 1876 2263 3814 6645 7432 7742
Size: 157 1393 326 387 1551 2831 787 310
Cuts at: 7742 7770
Size: 28
Fragments arranged by size:
2831 1551 1393 787 387 326 310 157
28
NarI GG'CG_CC
Cuts at: 0 7736 7770
Size: 7736 34
NcoI C'CATG_G
Cuts at: 0 1228 6836 7503 7770
Size: 1228 5608 667 267
Fragments arranged by size:
5608 1228 667 267
NdeI CA'TA_TG
Cuts at: 0 656 4888 7770
Size: 656 4232 2882
Fragments arranged by size:
4232 2882 656
NheI G'CTAG_C
Cuts at: 0 5629 7770
Size: 5629 2141
NsiI A_TGCA'T
Cuts at: 0 1652 7770
Size: 1652 6118
NspI r_CATG'y
Cuts at: 0 2726 5978 6942 6949 7232 7770
Size: 2726 3252 964 7 283 538
Fragments arranged by size:
3252 2726 964 538 283 7
PflMI CCAn_nnn'nTGG
Cuts at: 0 3814 7770
Size: 3814 3956
PfoI T'CCnGG_A
Cuts at: 0 314 1160 4917 7770
Size: 314 846 3757 2853
Fragments arranged by size:
3757 2853 846 314
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2408 2440 7770
Size: 2408 32 5330
Fragments arranged by size:
5330 2408 32
PsiI TTA'TAA
Cuts at: 0 1085 1421 2792 7770
Size: 1085 336 1371 4978
Fragments arranged by size:
4978 1371 1085 336
PstI C_TGCA'G
Cuts at: 0 162 2663 5753 6291 6912 7770
Size: 162 2501 3090 538 621 858
Fragments arranged by size:
3090 2501 858 621 538 162
PvuII CAG'CTG
Cuts at: 0 157 1550 1876 2263 3814 7432 7742 7770
Size: 157 1393 326 387 1551 3618 310 28
Fragments arranged by size:
3618 1551 1393 387 326 310 157 28
SalI G'TCGA_C
Cuts at: 0 1397 7770
Size: 1397 6373
SapI GCTCTTCn'nnn_
Cuts at: 0 4060 7770
Size: 4060 3710
ScaI AGT'ACT
Cuts at: 0 3803 4790 6244 6285 7770
Size: 3803 987 1454 41 1485
Fragments arranged by size:
3803 1485 1454 987 41
SexAI A'CCwGG_T
Cuts at: 0 2384 5301 7770
Size: 2384 2917 2469
Fragments arranged by size:
2917 2469 2384
SfcI C'TryA_G
Cuts at: 0 158 176 2659 4181 4751 5749 6287 6713
Size: 158 18 2483 1522 570 998 538 426
Cuts at: 6713 6815 6908 7770
Size: 102 93 862
Fragments arranged by size:
2483 1522 998 862 570 538 426 158
102 93 18
SfoI GGC'GCC
Cuts at: 0 7737 7770
Size: 7737 33
SmlI C'TyrA_G
Cuts at: 0 1073 2199 4174 5454 6809 7770
Size: 1073 1126 1975 1280 1355 961
Fragments arranged by size:
1975 1355 1280 1126 1073 961
SpeI A'CTAG_T
Cuts at: 0 3451 6535 6786 7770
Size: 3451 3084 251 984
Fragments arranged by size:
3451 3084 984 251
SphI G_CATG'C
Cuts at: 0 5978 6942 7232 7770
Size: 5978 964 290 538
Fragments arranged by size:
5978 964 538 290
SspI AAT'ATT
Cuts at: 0 2475 2828 3861 6744 7770
Size: 2475 353 1033 2883 1026
Fragments arranged by size:
2883 2475 1033 1026 353
StuI AGG'CCT
Cuts at: 0 1750 6349 6827 7770
Size: 1750 4599 478 943
Fragments arranged by size:
4599 1750 943 478
StyI C'CwwG_G
Cuts at: 0 132 1228 4806 5275 5693 5942 6078 6453
Size: 132 1096 3578 469 418 249 136 375
Cuts at: 6453 6836 7419 7503 7770
Size: 383 583 84 267
Fragments arranged by size:
3578 1096 583 469 418 383 375 267
249 136 132 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2577 5229 7550 7770
Size: 2577 2652 2321 220
Fragments arranged by size:
2652 2577 2321 220
TatI w'GTAC_w
Cuts at: 0 1633 1789 2719 3801 4788 6242 6283 7770
Size: 1633 156 930 1082 987 1454 41 1487
Fragments arranged by size:
1633 1487 1454 1082 987 930 156 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2685 2717 3015 3047 5518 5550 7770
Size: 2685 32 298 32 2471 32 2220
Fragments arranged by size:
2685 2471 2220 298 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3213 7770
Size: 3213 4557
XbaI T'CTAG_A
Cuts at: 0 1404 3865 4956 7770
Size: 1404 2461 1091 2814
Fragments arranged by size:
2814 2461 1404 1091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1040 7770
Size: 1040 6730
XhoI C'TCGA_G
Cuts at: 0 1073 7770
Size: 1073 6697
XmnI GAAnn'nnTTC
Cuts at: 0 3803 4424 4535 6203 6862 7770
Size: 3803 621 111 1668 659 908
Fragments arranged by size:
3803 1668 908 659 621 111
Enzymes that do cut:
AccI Acc65I AcuI AflII AleI AlfI AlwNI ApoI
AseI AvaI BaeI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII EaeI
EarI Eco57MI EcoNI EcoRI EcoRV FalI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NsiI NspI
PflMI PfoI PpiI PsiI PstI PvuII SalI SapI
ScaI SexAI SfcI SfoI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI AloI
ApaI ApaLI AscI AsiSI AvrII BamHI BcgI BplI
BsaAI BsaWI BsiEI BsiWI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstEII BstZ17I DrdI EagI EciI EcoICRI
EcoO109I FseI FspI FspAI MluI NaeI NgoMIV NotI
NruI PacI PasI PciI PmeI PmlI PpuMI PshAI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SanDI
SbfI SfiI SgfI SgrAI SmaI SnaBI SrfI SwaI
XmaI ZraI