Restriction map for
pF1KE2700
(Linear) (Six-base) MAPSORT of: KIEE2700.seq Check: 5169 from: 1 to: 1560
KIEE2700.orf
With 182 enzymes: SgfI *
July 6, 2017 13:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1205 1560
Size: 1205 355
Acc65I G'GTAC_C
Cuts at: 0 1290 1560
Size: 1290 270
AfeI AGC'GCT
Cuts at: 0 726 1560
Size: 726 834
AlwNI CAG_nnn'CTG
Cuts at: 0 253 388 1342 1560
Size: 253 135 954 218
Fragments arranged by size:
954 253 218 135
ApaI G_GGCC'C
Cuts at: 0 720 1560
Size: 720 840
AvaI C'yCGr_G
Cuts at: 0 1242 1560
Size: 1242 318
BanI G'GyrC_C
Cuts at: 0 343 361 1078 1290 1560
Size: 343 18 717 212 270
Fragments arranged by size:
717 343 270 212 18
BanII G_rGCy'C
Cuts at: 0 720 826 1069 1560
Size: 720 106 243 491
Fragments arranged by size:
720 491 243 106
BbsI GAAGACnn'nnnn_
Cuts at: 0 1095 1230 1560
Size: 1095 135 330
Fragments arranged by size:
1095 330 135
Bme1580I G_kGCm'C
Cuts at: 0 364 466 720 1560
Size: 364 102 254 840
Fragments arranged by size:
840 364 254 102
BmrI ACTGGGnnnn_n'
Cuts at: 0 1385 1560
Size: 1385 175
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 87 1560
Size: 87 1473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 254 301 619 668 854 898 1560
Size: 197 57 47 318 49 186 44 662
Fragments arranged by size:
662 318 197 186 57 49 47 44
Bpu10I CC'TnA_GC
Cuts at: 0 820 1560
Size: 820 740
BsaI GGTCTCn'nnnn_
Cuts at: 0 414 1560
Size: 414 1146
BsaWI w'CCGG_w
Cuts at: 0 1418 1560
Size: 1418 142
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 703 1560
Size: 328 375 857
Fragments arranged by size:
857 375 328
BseYI C'CCAG_C
Cuts at: 0 1039 1560
Size: 1039 521
Bsp1286I G_dGCh'C
Cuts at: 0 364 466 720 826 1069 1560
Size: 364 102 254 106 243 491
Fragments arranged by size:
491 364 254 243 106 102
BspEI T'CCGG_A
Cuts at: 0 1418 1560
Size: 1418 142
BspHI T'CATG_A
Cuts at: 0 299 1560
Size: 299 1261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1205 1560
Size: 1205 355
BsrBI CCG'CTC
Cuts at: 0 45 222 1560
Size: 45 177 1338
Fragments arranged by size:
1338 177 45
BsrDI GCAATG_nn'
Cuts at: 0 122 1239 1560
Size: 122 1117 321
Fragments arranged by size:
1117 321 122
BssSI C'ACGA_G
Cuts at: 0 1045 1560
Size: 1045 515
BstAPI GCAn_nnn'nTGC
Cuts at: 0 388 1560
Size: 388 1172
BstXI CCAn_nnnn'nTGG
Cuts at: 0 760 1560
Size: 760 800
BstYI r'GATC_y
Cuts at: 0 443 1502 1560
Size: 443 1059 58
Fragments arranged by size:
1059 443 58
Bsu36I CC'TnA_GG
Cuts at: 0 356 1560
Size: 356 1204
BtgI C'CryG_G
Cuts at: 0 130 307 482 653 1436 1560
Size: 130 177 175 171 783 124
Fragments arranged by size:
783 177 175 171 130 124
BtsI GCAGTG_nn'
Cuts at: 0 464 1560
Size: 464 1096
DrdI GACnn_nn'nnGTC
Cuts at: 0 648 1273 1560
Size: 648 625 287
Fragments arranged by size:
648 625 287
EaeI y'GGCC_r
Cuts at: 0 332 1383 1444 1560
Size: 332 1051 61 116
Fragments arranged by size:
1051 332 116 61
EarI CTCTTCn'nnn_
Cuts at: 0 155 1363 1560
Size: 155 1208 197
Fragments arranged by size:
1208 197 155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 254 301 619 668 854 898 1560
Size: 197 57 47 318 49 186 44 662
Fragments arranged by size:
662 318 197 186 57 49 47 44
EcoO109I rG'GnC_Cy
Cuts at: 0 325 1339 1560
Size: 325 1014 221
Fragments arranged by size:
1014 325 221
HaeII r_GCGC'y
Cuts at: 0 728 1560
Size: 728 832
HincII GTy'rAC
Cuts at: 0 1278 1560
Size: 1278 282
KpnI G_GTAC'C
Cuts at: 0 1294 1560
Size: 1294 266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 575 1560
Size: 268 307 985
Fragments arranged by size:
985 307 268
MscI TGG'CCA
Cuts at: 0 1385 1560
Size: 1385 175
MslI CAynn'nnrTG
Cuts at: 0 658 1560
Size: 658 902
MspA1I CmG'CkG
Cuts at: 0 1452 1560
Size: 1452 108
NcoI C'CATG_G
Cuts at: 0 130 482 653 1436 1560
Size: 130 352 171 783 124
Fragments arranged by size:
783 352 171 130 124
PflMI CCAn_nnn'nTGG
Cuts at: 0 1182 1404 1560
Size: 1182 222 156
Fragments arranged by size:
1182 222 156
PfoI T'CCnGG_A
Cuts at: 0 800 962 1262 1560
Size: 800 162 300 298
Fragments arranged by size:
800 300 298 162
PpuMI rG'GwC_Cy
Cuts at: 0 1339 1560
Size: 1339 221
PshAI GACnn'nnGTC
Cuts at: 0 983 1136 1560
Size: 983 153 424
Fragments arranged by size:
983 424 153
PspOMI G'GGCC_C
Cuts at: 0 716 1560
Size: 716 844
PstI C_TGCA'G
Cuts at: 0 384 1004 1560
Size: 384 620 556
Fragments arranged by size:
620 556 384
PvuII CAG'CTG
Cuts at: 0 1452 1560
Size: 1452 108
ScaI AGT'ACT
Cuts at: 0 1087 1560
Size: 1087 473
SexAI A'CCwGG_T
Cuts at: 0 1341 1560
Size: 1341 219
SfcI C'TryA_G
Cuts at: 0 380 990 1000 1560
Size: 380 610 10 560
Fragments arranged by size:
610 560 380 10
SmaI CCC'GGG
Cuts at: 0 1244 1560
Size: 1244 316
StyI C'CwwG_G
Cuts at: 0 130 398 482 653 1403 1436 1560
Size: 130 268 84 171 750 33 124
Fragments arranged by size:
750 268 171 130 124 84 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 411 1560
Size: 411 1149
TatI w'GTAC_w
Cuts at: 0 875 1085 1541 1560
Size: 875 210 456 19
Fragments arranged by size:
875 456 210 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 800 832 1244 1276 1448 1480 1560
Size: 800 32 412 32 172 32 80
Fragments arranged by size:
800 412 172 80 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 17 490 1560
Size: 17 473 1070
Fragments arranged by size:
1070 473 17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 91 1560
Size: 91 1469
XmaI C'CCGG_G
Cuts at: 0 1242 1560
Size: 1242 318
XmnI GAAnn'nnTTC
Cuts at: 0 1483 1560
Size: 1483 77
Enzymes that do cut:
AarI Acc65I AfeI AlwNI ApaI AvaI BanI BanII
BbsI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaWI
BseRI BseYI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DrdI
EaeI EarI Eco57MI EcoO109I HaeII HincII KpnI MmeI
MscI MslI MspA1I NcoI PflMI PfoI PpuMI PshAI
PspOMI PstI PvuII ScaI SexAI SfcI SmaI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BpuEI BsaAI
BsaBI BsaHI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BsrFI BsrGI BssHII BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII
HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI XhoI ZraI